Delayed leaf senescence induces extreme drought tolerance in crop plants (2009)
Rivero, Rosa, Peleg, Zvi, Szczerba, Mark, Tumimbang, Ellen, Jauregui, Rosa N, Liu, Li, ...
Drought, the most prominent threat to agricultural production worldwide, accelerates leaf senescence, leading to a decrease in canopy size, loss in photosynthesis and reduced yields. On the basis of...
A Grape Berry (Vitis vinifera L.) Cation/Proton Antiporter is Associated with Berry Ripening (2007)
Hanana, Mohsen, Cagnac, Olivier, Yamaguchi, Toshio, Hamdi, Saïd, Ghorbel, Abdelwahed, Blumwald, Eduardo
We have cloned and characterized VvNHX1, a gene encoding a vacuolar cation/H+ antiporter from Vitis vinifera cv. Cabernet Sauvignon. VvNHX1 belongs to the vacuolar NHX protein family and showed high...
Eigenheer, Richard A, Jin Lee, Young, Blumwald, Eduardo, Phinney, Brett S, Gelli, Angie
Extracellular proteins of Cryptococcus neoformans are involved in the pathogenesis of cryptococcosis, and some are immunoreactive antigens that may potentially serve as candidates for vaccine...
Sottosanto, Jordan B, Saranga, Yehoshua, Blumwald, Eduardo
Abstract Background AtNHX1, the most abundant vacuolar Na + /H + antiporter in Arabidopsis thaliana , mediates the transport of Na + and K + into the vacuole, influencing plant development and...
Novak, Jaroslav P, Kim, Seon-Young, Xu, Jun, Modlich, Olga, Volsky, David J, Honys, David, ...
Abstract Background DNA microarrays are a powerful technology that can provide a wealth of gene expression data for disease studies, drug development, and a wide scope of other investigations....
Novak, Jaroslav P., Kim, Seon-Young, Xu, Jun, Modlich, Olga, Volsky, David J., Honys, David, ...
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and...
He, Cixin, Yan, Juqiang, Shen, Guoxin, Fu, Lianhai, Holaday, A. Scott, Auld, Dick, ...
Drought and salinity are two major limiting factors in crop productivity. One way to reduce crop loss caused by drought and salinity is to increase the solute concentration in the vacuoles of plant...
He, Cixin, Yan, Juqiang, Shen, Guoxin, Fu, Lianhai, Holaday, A. Scott, Auld, Dick, ...
Drought and salinity are two major limiting factors in crop productivity. One way to reduce crop loss caused by drought and salinity is to increase the solute concentration in the vacuoles of plant...
He, Cixin, Yan, Juqiang, Shen, Guoxin, Fu, Lianhai, Holaday, A. Scott, Auld, Dick, ...
Drought and salinity are two major limiting factors in crop productivity. One way to reduce crop loss caused by drought and salinity is to increase the solute concentration in the vacuoles of plant...
Sottosanto, J B, Gelli, A, Blumwald, Eduardo
AtNHX1, a vacuolar cation/proton antiporter of Arabidopsis, plays an important role in salt tolerance, ion homeostasis and development. We used the T-DNA insertional mutant of AtNHX1 (nhx1 plants)...
Xing, Ti, Higgins, Verna J., Blumwald, Eduardo
Tomato cells (with the Cf-5 resistance gene) were treated with elicitor preparations containing the avr5 gene product from two Cf-5 incompatible races of the fungal pathogen Cladosporium fulvum (race...
Zhang, Hong-Xia, Hodson, Joanna N., Williams, John P., Blumwald, Eduardo
Transgenic Brassica napus plants overexpressing AtNHX1, a vacuolar Na+/H+ antiport from Arabidopsis thaliana, were able to grow, flower, and produce seeds in the presence of 200 mM sodium chloride....
Role of SH3 Domain–Containing Proteins in Clathrin-Mediated Vesicle Trafficking in Arabidopsis
Lam, Bernard C.-H., Sage, Tammy L., Bianchi, Fabrizio, Blumwald, Eduardo
A group of plant AtSH3Ps (Arabidopsis thaliana SH3-containing proteins) involved in trafficking of clathrin-coated vesicles was identified from the GenBank database. These proteins contained...
Yamaguchi, Toshio, Apse, Maris P., Shi, Huazhong, Blumwald, Eduardo
We conducted an analysis of the topology of AtNHX1, an Arabidopsis thaliana vacuolar Na+/H+ antiporter. Several hydrophilic regions of the antiporter were tagged with a hemagglutinin epitope, and...
Studies of osmoregulation in salt adaptation of cyanobacteria with ESR spin-probe techniques
Blumwald, Eduardo, Mehlhorn, Rolf J., Packer, Lester
Sucrose is accumulated in response to NaCl-induced stress in the cyanobacterium Synechococcus 6311. Internal cell volume was measured by ESR spectra with 2,2,6,6-tetramethyl-4-oxopiperidinoxy free...
Blumwald, Eduardo, Poole, Ronald J.
The fluorescent probes acridine orange and oxonol-V were used as indicators of pH gradients (ΔpH) and membrane potential differences (ΔΨ), respectively, in membrane vesicles believed to be derived...
Inhibition of Na+/H+ Antiport Activity in Sugar Beet Tonoplast by Analogs of Amiloride 1
Blumwald, Eduardo, Cragoe, Edward J., Poole, Ronald J.
The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension...
Blumwald, Eduardo, Poole, Ronald J.
Cell suspension cultures of sugar beet were grown at various salinities (0-200 millimolar NaCl). Their tolerance to Na+ was comparable to that of the intact plant. Tonoplast vesicles were prepared by...
Barkla, Bronwyn J., Charuk, Jeffrey H. M., Cragoe, Edward J., Blumwald, Eduardo
The effects of 5-(N-methyl-N-isobutyl)-amiloride (MIA), an amiloride analog, was tested on the Na+/H+ antiport activity of intact vacuoles and tonoplast vesicles isolated from sugar beet (Beta...
Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris1
Blumwald, Eduardo, Poole, Ronald J.
The pH-dependent fluorescence quenching of acridine orange was used to study the Na+- and K+-dependent H+ fluxes in tonoplast vesicles isolated from storage tissue of red beet and sugar beet (Beta...
Blumwald, Eduardo, Fortin, Marc G., Rea, Philip A., Verma, Desh Pal S., Poole, Ronald J.
An improved method is described for the isolation of membrane envelope enclosing the bacteroids (peribacteroid membrane) from soybean (Glycine max L.) root nodules. The ATPase activity of the...
Ionic Osmoregulation during Salt Adaptation of the Cyanobacterium Synechococcus 6311 1
Blumwald, Eduardo, Mehlhorn, Rolf J., Packer, Lester
The mechanisms of salt adaptation were studied in the cyanobacterium Synechococcus 6311. Intracellular volumes and ion concentrations were measured before and after abrupt increases of external NaCl...
Blumwald, Eduardo, Tel-Or, Elisha
Physiological aspects of salt-adaptation in the cyanobacterium Synechococcus 6311 growing in a continuous culture (turbidostat) were studied. The process of salt-adaptation was completed within 3...
Blumwald, Eduardo, Poole, Ronald J.
Artificial pH gradients across tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were used to study the kinetics of a Ca2+/H+ antiport across this membrane....
Griffith, Christopher J., Rea, Philip A., Blumwald, Eduardo, Poole, Ronald J.
The H+-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl− and NO3−. N-Ethylmaleimide (NEM) was employed...
Characterization of Vacuolar Malate and K+ Channels under Physiological Conditions 1
Pantoja, Omar, Gelli, Angie, Blumwald, Eduardo
Patch-clamp techniques were employed to study the electrical properties of vacuoles from sugar beet (Beta vulgaris) cell suspensions at physiological concentrations of cytoplasmic Ca2+. Vacuoles...
Tonoplast Ion Channels from Sugar Beet Cell Suspensions 1: Inhibition by Amiloride and Its Analogs
Pantoja, Omar, Dainty, Jack, Blumwald, Eduardo
The properties of the vacuolar membrane (tonoplast) ion channels of sugar beet (Beta vulgaries) cell cultures were studied using the patch-clamp technique. Tonoplast currents displayed inward...
Vera-Estrella, Rosario, Blumwald, Eduardo, Higgins, Verna J.
Intercellular fluid (IF) obtained from tomato (Lycopersicon esculentum L.) leaflets colonized by Cladosporium fulvum Cooke contains specific elicitors that induce necrosis in tomato cultivars...
Yamaguchi, Toshio, Aharon, Gilad S., Sottosanto, Jordan B., Blumwald, Eduardo
The selective movement of ions between intracellular compartments is fundamental for eukaryotes. Arabidopsis thaliana Na+/H+ exchanger 1 (AtNHX1), the most abundant vacuolar Na+/H+ antiporter in A....
Generalization of DNA microarray dispersion properties: microarray equivalent of t-distribution
Novak, Jaroslav P, Kim, Seon-Young, Xu, Jun, Modlich, Olga, Volsky, David J, Honys, David, ...
Zhang, Hong-Xia, Hodson, Joanna N., Williams, John P., Blumwald, Eduardo
Transgenic Brassica napus plants overexpressing AtNHX1, a vacuolar Na+/H+ antiport from Arabidopsis thaliana, were able to grow, flower, and produce seeds in the presence of 200 mM sodium chloride....
Role of SH3 Domain–Containing Proteins in Clathrin-Mediated Vesicle Trafficking in Arabidopsis
Lam, Bernard C.-H., Sage, Tammy L., Bianchi, Fabrizio, Blumwald, Eduardo
A group of plant AtSH3Ps (Arabidopsis thaliana SH3-containing proteins) involved in trafficking of clathrin-coated vesicles was identified from the GenBank database. These proteins contained...
Yamaguchi, Toshio, Apse, Maris P., Shi, Huazhong, Blumwald, Eduardo
We conducted an analysis of the topology of AtNHX1, an Arabidopsis thaliana vacuolar Na+/H+ antiporter. Several hydrophilic regions of the antiporter were tagged with a hemagglutinin epitope, and...
Studies of osmoregulation in salt adaptation of cyanobacteria with ESR spin-probe techniques
Blumwald, Eduardo, Mehlhorn, Rolf J., Packer, Lester
Sucrose is accumulated in response to NaCl-induced stress in the cyanobacterium Synechococcus 6311. Internal cell volume was measured by ESR spectra with 2,2,6,6-tetramethyl-4-oxopiperidinoxy free...
Blumwald, Eduardo, Poole, Ronald J.
The fluorescent probes acridine orange and oxonol-V were used as indicators of pH gradients (ΔpH) and membrane potential differences (ΔΨ), respectively, in membrane vesicles believed to be derived...
Inhibition of Na+/H+ Antiport Activity in Sugar Beet Tonoplast by Analogs of Amiloride 1
Blumwald, Eduardo, Cragoe, Edward J., Poole, Ronald J.
The effects of amiloride and a series of amiloride analogs have been tested on the Na+/H+ antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension...
Blumwald, Eduardo, Poole, Ronald J.
Cell suspension cultures of sugar beet were grown at various salinities (0-200 millimolar NaCl). Their tolerance to Na+ was comparable to that of the intact plant. Tonoplast vesicles were prepared by...
Barkla, Bronwyn J., Charuk, Jeffrey H. M., Cragoe, Edward J., Blumwald, Eduardo
The effects of 5-(N-methyl-N-isobutyl)-amiloride (MIA), an amiloride analog, was tested on the Na+/H+ antiport activity of intact vacuoles and tonoplast vesicles isolated from sugar beet (Beta...
Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris1
Blumwald, Eduardo, Poole, Ronald J.
The pH-dependent fluorescence quenching of acridine orange was used to study the Na+- and K+-dependent H+ fluxes in tonoplast vesicles isolated from storage tissue of red beet and sugar beet (Beta...
Blumwald, Eduardo, Fortin, Marc G., Rea, Philip A., Verma, Desh Pal S., Poole, Ronald J.
An improved method is described for the isolation of membrane envelope enclosing the bacteroids (peribacteroid membrane) from soybean (Glycine max L.) root nodules. The ATPase activity of the...
Ionic Osmoregulation during Salt Adaptation of the Cyanobacterium Synechococcus 6311 1
Blumwald, Eduardo, Mehlhorn, Rolf J., Packer, Lester
The mechanisms of salt adaptation were studied in the cyanobacterium Synechococcus 6311. Intracellular volumes and ion concentrations were measured before and after abrupt increases of external NaCl...
Blumwald, Eduardo, Tel-Or, Elisha
Physiological aspects of salt-adaptation in the cyanobacterium Synechococcus 6311 growing in a continuous culture (turbidostat) were studied. The process of salt-adaptation was completed within 3...
Blumwald, Eduardo, Poole, Ronald J.
Artificial pH gradients across tonoplast vesicles isolated from storage tissue of red beet (Beta vulgaris L.) were used to study the kinetics of a Ca2+/H+ antiport across this membrane....
Griffith, Christopher J., Rea, Philip A., Blumwald, Eduardo, Poole, Ronald J.
The H+-ATPase of tonoplast vesicles isolated from red beet (Beta vulgaris L.) storage tissue was studied with respect to the kinetic effects of Cl− and NO3−. N-Ethylmaleimide (NEM) was employed...
Characterization of Vacuolar Malate and K+ Channels under Physiological Conditions 1
Pantoja, Omar, Gelli, Angie, Blumwald, Eduardo
Patch-clamp techniques were employed to study the electrical properties of vacuoles from sugar beet (Beta vulgaris) cell suspensions at physiological concentrations of cytoplasmic Ca2+. Vacuoles...
Tonoplast Ion Channels from Sugar Beet Cell Suspensions 1: Inhibition by Amiloride and Its Analogs
Pantoja, Omar, Dainty, Jack, Blumwald, Eduardo
The properties of the vacuolar membrane (tonoplast) ion channels of sugar beet (Beta vulgaries) cell cultures were studied using the patch-clamp technique. Tonoplast currents displayed inward...
Vera-Estrella, Rosario, Blumwald, Eduardo, Higgins, Verna J.
Intercellular fluid (IF) obtained from tomato (Lycopersicon esculentum L.) leaflets colonized by Cladosporium fulvum Cooke contains specific elicitors that induce necrosis in tomato cultivars...
Yamaguchi, Toshio, Aharon, Gilad S., Sottosanto, Jordan B., Blumwald, Eduardo
The selective movement of ions between intracellular compartments is fundamental for eukaryotes. Arabidopsis thaliana Na+/H+ exchanger 1 (AtNHX1), the most abundant vacuolar Na+/H+ antiporter in A....
Generalization of DNA microarray dispersion properties: microarray equivalent of t-distribution
Novak, Jaroslav P, Kim, Seon-Young, Xu, Jun, Modlich, Olga, Volsky, David J, Honys, David, ...
Delayed leaf senescence induces extreme drought tolerance in a flowering plant
Rivero, Rosa M., Kojima, Mikiko, Gepstein, Amira, Sakakibara, Hitoshi, Mittler, Ron, Gepstein, Shimon, ...
Drought, the most prominent threat to agricultural production worldwide, accelerates leaf senescence, leading to a decrease in canopy size, loss in photosynthesis and reduced yields. On the basis of...
Rivero, Rosa M., Shulaev, Vladimir, Blumwald, Eduardo
We investigated the effects of PSARK∷IPT (for Senescence-Associated Receptor Kinase∷Isopentenyltransferase) expression and cytokinin production on several aspects of photosynthesis in transgenic...