Boekema, Egbert J., Trinkunas, Gediminas, Kereiche, Sami, Broess, Koen L., Caffarri, Stefano, Amerongen, Herbert Van, ...
Kouřil, Roman, Boekema, Egbert J., Braun, Hans-Peter, Bultema, Jelle B.
The individual protein complexes of the oxidative phosphorylation system (OXPHOS complexes I to V) specifically interact and form defined supramolecular structures, the so-called “respiratory...
Boekema, Egbert J., Schouten, Stefan, Folea, Mihaela, Schwarz, Heinz, Hobel, Cedric F.V., Pitcher, Angela, ...
The crenarchaea Sulfolobus acidocaldarius, S. solfataricus and S. tokodaii, release membrane vesicles into the medium. These membrane vesicles consist of tetraether lipids and are coated with an...
Braun, Hans-Peter, Sunderhaus, Stephanie, Boekema, Egbert J., Kouřil, Roman
The protein complexes of the respiratory chain interact by forming large protein particles called respiratory supercomplexes or ‘‘respirasomes’’. Biochemical characterization of these...
The Role of Lhca Complexes in the Supramolecular Organization of Higher Plant Photosystem I (2009)
Wientjes, Emilie, Oostergetel, Gert T., Jansson, Stefan, Boekema, Egbert J., Croce, Roberta
In this work, Photosystem I supercomplexes have been purified from Lhca-deficient lines of Arabidopsis thaliana using a mild detergent treatment that does not induce loss of outer antennas. The...
Arteni, Ana A., Ajlani, Ghada, Boekema, Egbert J.
In cyanobacteria, the harvesting of light energy for photosynthesis is mainly carried out by the phycobilisome—a giant, multi-subunit pigment–protein complex. This complex is composed of...
Alternating syn-anti bacteriochlorophylls form concentric helical nanotubes in chlorosomes (2009)
Ganapathy, Swapna, Oostergetel, Gert T., Wawrzyniak, Piotr K., Reus, Michael, Gomez Maqueo Chew, Aline, Buda, Francesco, ...
Chlorosomes are the largest and most efficient light-harvesting antennae found in nature, and they are constructed from hundreds of thousands of self-assembled bacteriochlorophyll (BChl) c, d, or e...
Mościcka, Katarzyna B., Krupnik, Tomasz, Boekema, Egbert J., Lolkema, Juke S.
The structure of three secondary transporter proteins, GltT of Bacillus stearothermophilus, CitS of Klebsiella pneumoniae, and GltS of Escherichia coli, was studied. The proteins were purified to...
Folea, I. Mihaela, Zhang, Pengpeng, Nowaczyk, Marc M., Ogawa, Teruo, Aro, Eva-Mari, Boekema, Egbert J.
The larger protein complexes of the cyanobacterial photosynthetic membrane of Thermosynechoccus elongatus and Synechocystis 6803 were studied by single particle electron microscopy after detergent...
Arteni, Ana A., Liu, Lu-Ning, Aartsma, Thijs J., Zhang, Yu-Zhong, Zhou, Bai-Cheng, Boekema, Egbert J.
In the present work, electron microscopy and single particle averaging was performed to investigate the supramolecular architecture of hemiellipsoidal phycobilisomes from the unicellular red alga...
Peters, Katrin, Dudkina, Natalya V., Jänsch, Lothar, Braun, Hans-Peter, Boekema, Egbert J.
The projection structures of complex I and the I+III2 supercomplex from the C4 plant Zea mays were determined by electron microscopy and single particle image analysis to a resolution of up to 11 Å....
Folea, I. Mihaela, Zhang, Pengpeng, Aro, Eva-Mari, Boekema, Egbert J.
The supramolecular organization of photosystem II (PSII) complexes in the photosynthetic membrane of the cyanobacterium Synechocystis 6803 was studied by electron microscopy. After mild detergent...
Structural characterization of respiratory complexes in potato tuber (2008)
Bultema, Jelle B., Kouřil, Roman, Braun, Hans-Peter, Boekema, Egbert J.
Supramolecular organization of mitochondrial ATP synthases: Electron microscopy study (2008)
Dudkina, Natalya V., Oostergetel, Gert, Keegstra, Wilko, Heinemeyer, Jesco, Braun, Hans-Peter, Boekema, Egbert J.
Kereïche, Sami, Kouřil, Roman, Oostergetel, Gert T., Fusetti, Fabrizia, Boekema, Egbert J., Doust, Alexander B., ...
Photosynthetic supercomplexes from the cryptophyte Rhodomonas CS24 were isolated by a short detergent treatment of membranes from the cryptophyte Rhodomonas CS24 and studied by electron microscopy...
Kereïche, Sami, Bourinet, Laurent, Keegstra, Wilko, Arteni, Ana A., Verbavatz, Jean-Marc, Boekema, Egbert J., ...
The integral membrane light-harvesting (LH) proteins from purple photosynthetic bacteria form circular oligomers of an elementary unit that is composed of two very hydrophobic polypeptides, termed α...
Fröls, Sabrina, Ajon, Malgorzata, Wagner, Michaela, Teichmann, Daniela, Zolghadr, Behnam, Folea, Mihaela, ...
The hyperthermophilic archaeon Sulfolobus solfataricus has been shown to exhibit a complex transcriptional response to UV irradiation involving 55 genes. Among the strongest UV-induced genes was a...
Botteaux, Anne, Sani, Musa, Kayath, Christian A., Boekema, Egbert J., Allaoui, Abdelmounaaïm
The effectors of enterocyte invasion by Shigella are dependent on a type III secretion system that contains a needle whose length average does not exceed 50 nm. Previously, we reported that Spa32 is...
Dudkina, Natalya V., Sunderhaus, Stephanie, Boekema, Egbert J., Braun, Hans-Peter
The organization of the oxidative phosphorylation (OXPHOS) system within the inner mitochondrial membrane appears to be far more complicated than previously thought. In particular, the individual...
Supramolecular Structure of the Mitochondrial Oxidative Phosphorylation System (2007)
Braun, Hans-Peter, Boekema, Egbert J.
The protein complexes of the mitochondrial oxidative phosphorylation system were recently reported to form supramolecular assemblies termed respiratory supercomplexes or respirasomes. These...
IpaD is localized at the tip of the Shigella flexneri type III secretion apparatus (2007)
Sani, Musa, Botteaux, Anne, Parsot, Claude, Sansonetti, Philippe, Boekema, Egbert J., Allaoui, Abdelmounaaïm
Type III secretion (T3S) systems are used by numerous Gram-negative pathogenic bacteria to inject virulence proteins into animal and plant host cells. The core of the T3S apparatus, known as the...
Sani, Musa, Allaoui, Abdelmounaaïm, Fusetti, Fabrizia, Oostergetel, Gert T., Keegstra, Wilko, Boekema, Egbert J.
The secretion apparatus known as the needle complex (NC) from the bacterium Shigella flexneri was studied by single particle electron microscopy. The isolated intact NC appears in projection to be...
Moscicka, Katarzyna B., Klompmaker, Sandra H., Wang, Dongyuan, Boekema, Egbert J.
We have studied Hansenula polymorpha Pex5p and Pex20p, peroxins involved in peroxisomal matrix protein import. In vitro binding experiments suggested that H. polymorpha Pex5p and Pex20p physically...
Structure, function and regulation of plant photosystem I (2007)
Jensen, Poul Erik, Bassi, Roberto, Boekema, Egbert J., Dekker, Jan P., Jansson, Stefan, Leister, Dario, ...
Photosystem I (PSI) is a multisubunit protein complex located in the thylakoid membranes of green plants and algae, where it initiates one of the first steps of solar energy conversion by...
Lax, Julia E.-M., Arteni, Ana A., Boekema, Egbert J., Pistorius, Elfriede K., Michel, Klaus-Peter, Rögner, Matthias
Iron deficiency triggers various processes in cyanobacterial cells of which the synthesis of an additional antenna system (IsiA) around photosystem (PS) 1 is well documented. Here we show that PS2...
A Structural Model of the Cytochrome c Reductase/Oxidase Supercomplex from Yeast Mitochondria (2007)
Heinemeyer, Jesco, Braun, Hans-Peter, Boekema, Egbert J., Kouřil, Roman
Mitochondrial respiratory chain complexes are arranged in supercomplexes within the inner membrane. Interaction of cytochrome c reductase (complex III) and cytochrome c oxidase (complex IV) was...
Szabó, Zalán, Sani, Musa, Groeneveld, Maarten, Zolghadr, Benham, Schelert, James, Albers, Sonja-Verena, ...
Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed...
Stuart, Marc C.A., Boekema, Egbert J.
The detergent solubilization and reformation of phospholipid vesicles was studied for various detergents. Two distinct mechanisms of vesicle-to-micelle and micelle-to-vesicle transition were observed...
Oostergetel, Gert T., Reus, Michael, Gomez Maqueo Chew, Aline, Bryant, Donald A., Boekema, Egbert J., Holzwarth, Alfred R.
Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers....
Boekema, Egbert J., Aro, Eva-Mari, Ogawa, Teruo, Battchikova, Natalia, Zhang, Pengpeng, Arteni, Ana A.
The structure of the multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes from cyanobacteria was investigated by growing the wild type and specific ndh His-tag mutants of...
Boekema, Egbert J., Braun, Hans-Peter, Sunderhaus, Stephanie, Dudkina, Natalya V.
There is increasing evidence now that F1F0 ATP synthase is arranged in dimers in the inner mitochondrial membrane of several organisms. The dimers are also considered to be the building blocks of...
Braun, Hans-Peter, Boekema, Egbert J., Zabaleta, Eduardo, Perales, Mariano, Heinemeyer, Jesco, Klodmann, Jennifer, ...
Complex I of Arabidopsis includes five structurally related subunits representing γ-type carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these subunits within complex I was...
Respiratory chain supercomplexes in the plant mitochondrial membrane (2006)
Braun, Hans-Peter, Boekema, Egbert J., Sunderhaus, Stephanie, Heinemeyer, Jesco, Dudkina, Natalya V.
The intricate, heavily folded inner membrane of mitochondria houses the respiratory chain complexes. These complexes, together with the ATP synthase complex, are responsible for energy production,...
Jansson, Stefan, Boekema, Egbert J., Ruban, Alexander V., Ilioaia, Cristian, Kereïche, Sami, Damkjær, Jakob, ...
The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana plants depleted of one of...
Boekema, Egbert J., Braun, Hans-Peter, Sunderhaus, Stephanie, Dudkina, Natalya V.
There is increasing evidence now that F1F0 ATP synthase is arranged in dimers in the inner mitochondrial membrane of several organisms. The dimers are also considered to be the building blocks of...
Jänsch, Lothar, Dudkina, Natalya V., Sunderhaus, Stephanie, Braun, Hans-Peter, Boekema, Egbert J., Zabaleta, Eduardo, ...
Complex I of Arabidopsis includes five structurally related subunits representing γ-type carbonic anhydrases termed CA1, CA2, CA3, CAL1, and CAL2. The position of these subunits within complex I was...
Horton, Peter, Jansson, Stefan, Boekema, Egbert J., Ruban, Alexander V., Ilioaia, Cristian, Kereïche, Sami, ...
The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana plants depleted of one of...
Respiratory chain supercomplexes in the plant mitochondrial membrane (2006)
Braun, Hans-Peter, Boekema, Egbert J., Sunderhaus, Stephanie, Heinemeyer, Jesco, Dudkina, Natalya V.
The intricate, heavily folded inner membrane of mitochondria houses the respiratory chain complexes. These complexes, together with the ATP synthase complex, are responsible for energy production,...
Boekema, Egbert J., Aro, Eva-Mari, Ogawa, Teruo, Battchikova, Natalia, Zhang, Pengpeng, Arteni, Ana A.
The structure of the multifunctional NAD(P)H dehydrogenase type 1 (NDH-1) complexes from cyanobacteria was investigated by growing the wild type and specific ndh His-tag mutants of...
Aggregates of the Chlorophyll-Binding Protein IsiA (CP43') Dissipate Energy in Cyanobacteria (2005)
Grondelle, Rienk Van, Matthijs, Hans C.P., Arteni, Ana A., Boekema, Egbert J., Yeremenko, Nataliya, Roon, Henny Van, ...
In many natural habitats, growth of cyanobacteria may be limited by a low concentration of iron. Cyanobacteria respond to this condition by expressing a number of iron-stress-inducible genes, of...
Arteni, Ana A., Wit, Chantal D. De, Dekker, Jan P., Jensen, Poul Erik, Scheller, Henrik Vibe, Boekema, Egbert J., ...
Chloroplasts are central to the provision of energy for green plants. Their photosynthetic membrane consists of two major complexes converting sunlight: photosystem I (PSI) and photosystem II (PSII)....
Grüber, Gerhard, Boekema, Egbert J., Juliano, Sandra, Chaban, Yuriy L.
Projection maps of a V1–Vma5p hybrid complex, composed of subunit C (Vma5p) of Saccharomyces cerevisiae V-ATPase and the C-depleted V1 from Manduca sexta, were determined from single particle...
Supramolecular organization of thylakoid membrane proteins in green plants (2005)
Dekker, Jan P., Boekema, Egbert J.
The light reactions of photosynthesis in green plants are mediated by four large protein complexes, embedded in the thylakoid membrane of the chloroplast. Photosystem I (PSI) and Photosystem II...
Supercomplexes of IsiA and Photosystem I in a mutant lacking subunit PsaL (2005)
Dekker, Jan P., Matthijs, Hans C.P., Oostergetel, Gert T., Haene, Sandrine D’, Boekema, Egbert J., Yeremenko, Nataliya, ...
The cyanobacterium Synechocystis PCC 6803 grown under short-term iron-deficient conditions assembles a supercomplex consisting of a trimeric Photosystem I (PSI) complex encircled by a ring of 18 IsiA...
Braun, Hans-Peter, Boekema, Egbert J., Keegstra, Wilko, Heinemeyer, Jesco, Dudkina, Natalya V.
Respiration in all cells depends upon synthesis of ATP by the ATP synthase complex, a rotary motor enzyme. The structure of the catalytic moiety of ATP synthase, the so-called F1 headpiece, is well...
Boekema, Egbert J., Dekker, Jan P., Matthijs, Hans C.P., Rögner, Matthias, Haene, Sandrine D', Yeremenko, Nataliya, ...
Cyanobacteria express large quantities of the iron stress-inducible protein IsiA under iron deficiency. IsiA can assemble into numerous types of single or double rings surrounding Photosystem I....
Rögner, Matthias, Kouřil, Roman, Lax, Julia, Nowaczyk, Marc, Boekema, Egbert J., Arteni, Ana A.
Large data sets of molecular projections of the membrane proteins Photosystem I and Photosystem II from cyanobacteria were analyzed by single particle electron microscopy (EM). Analysis resulted in...
Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III (2005)
Braun, Hans-Peter, Boekema, Egbert J., Keegstra, Wilko, Eubel, Holger, Dudkina, Natalia V.
Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes:...
Photosystem I: a search for green plant trimers (2005)
Kouřil, Roman, Oosterwijk, Niels Van, Yakushevska, Alevtyna E., Boekema, Egbert J.
Recent blue-native gel electrophoresis studies gave evidence for the existence of dimeric and trimeric PSI complexes in green plants. We used single particle electron microscopy to investigate all...
Aggregates of the Chlorophyll-Binding Protein IsiA (CP43') Dissipate Energy in Cyanobacteria (2005)
Roon, Henny Van, Yeremenko, Nataliya, Haene, Sandrine D’, Ihalainen, Janne A., Arteni, Ana A., Dekker, Jan P., ...
In many natural habitats, growth of cyanobacteria may be limited by a low concentration of iron. Cyanobacteria respond to this condition by expressing a number of iron-stress-inducible genes, of...
Scheller, Henrik Vibe, Jensen, Poul Erik, Dekker, Jan P., Wit, Chantal D. De, Arteni, Ana A., Zygadlo, Agnieszka, ...
Chloroplasts are central to the provision of energy for green plants. Their photosynthetic membrane consists of two major complexes converting sunlight: photosystem I (PSI) and photosystem II (PSII)....
Grüber, Gerhard, Boekema, Egbert J., Juliano, Sandra, Chaban, Yuriy L.
Projection maps of a V1–Vma5p hybrid complex, composed of subunit C (Vma5p) of Saccharomyces cerevisiae V-ATPase and the C-depleted V1 from Manduca sexta, were determined from single particle...
Supramolecular organization of thylakoid membrane proteins in green plants (2005)
Boekema, Egbert J., Dekker, Jan P.
The light reactions of photosynthesis in green plants are mediated by four large protein complexes, embedded in the thylakoid membrane of the chloroplast. Photosystem I (PSI) and Photosystem II...
Supercomplexes of IsiA and Photosystem I in a mutant lacking subunit PsaL (2005)
Oostergetel, Gert T., Haene, Sandrine D’, Yeremenko, Nataliya, Kouřil, Roman, Boekema, Egbert J., Dekker, Jan P., ...
The cyanobacterium Synechocystis PCC 6803 grown under short-term iron-deficient conditions assembles a supercomplex consisting of a trimeric Photosystem I (PSI) complex encircled by a ring of 18 IsiA...
Braun, Hans-Peter, Boekema, Egbert J., Keegstra, Wilko, Heinemeyer, Jesco, Dudkina, Natalya V.
Respiration in all cells depends upon synthesis of ATP by the ATP synthase complex, a rotary motor enzyme. The structure of the catalytic moiety of ATP synthase, the so-called F1 headpiece, is well...
Matthijs, Hans C.P., Rögner, Matthias, Haene, Sandrine D', Yeremenko, Nataliya, Lax, Julia, Arteni, Ana A., ...
Cyanobacteria express large quantities of the iron stress-inducible protein IsiA under iron deficiency. IsiA can assemble into numerous types of single or double rings surrounding Photosystem I....
Boekema, Egbert J., Rögner, Matthias, Kouřil, Roman, Lax, Julia, Nowaczyk, Marc, Arteni, Ana A.
Large data sets of molecular projections of the membrane proteins Photosystem I and Photosystem II from cyanobacteria were analyzed by single particle electron microscopy (EM). Analysis resulted in...
Photosystem I: a search for green plant trimers (2005)
Boekema, Egbert J., Yakushevska, Alevtyna E., Oosterwijk, Niels Van, Kouřil, Roman
Recent blue-native gel electrophoresis studies gave evidence for the existence of dimeric and trimeric PSI complexes in green plants. We used single particle electron microscopy to investigate all...
Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III (2005)
Braun, Hans-Peter, Boekema, Egbert J., Keegstra, Wilko, Eubel, Holger, Dudkina, Natalia V.
Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes:...
Matthijs, Hans C.P., Boekema, Egbert J., Hagemann, Martin, Keegstra, Wilko, Dekker, Henk L., Andrizhiyevskaya, Elena G., ...
A significant part of global primary productivity is provided by cyanobacteria, which are abundant in most marine and freshwater habitats. In many oceanographic regions, however, the concentration of...
Structural Characterization of an ATPase Active F1-/V1-ATPase (α3β3EG) Hybrid Complex (2004)
Grüber, Gerhard, Boekema, Egbert J., Oostergetel, Gert T., Keegstra, Wilko, Coskun, Ünal, Chaban, Yuriy L.
Co-reconstitution of subunits E and G of the yeast V-ATPase and the α and β subunits of the F1-ATPase from the thermophilic Bacillus PS3 (TF1) resulted in an α3β3EG hybrid complex showing 53% of...
Boekema, Egbert J., Keegstra, Wilko, Müller, Volker, Lingl, Astrid, Chaban, Yuriy L., Coskun, Ünal, ...
In Archaea, bacteria, and eukarya, ATP provides metabolic energy for energy-dependent processes. It is synthesized by enzymes known as A-type or F-type ATP synthase, which are the smallest rotatory...
Haene, Sandrine D’, Ihalainen, Janne A., Kouřil, Roman, Yeremenko, Nataliya, Dekker, Jan P., Matthijs, Hans C.P., ...
A significant part of global primary productivity is provided by cyanobacteria, which are abundant in most marine and freshwater habitats. In many oceanographic regions, however, the concentration of...
Structural Characterization of an ATPase Active F1-/V1-ATPase (α3β3EG) Hybrid Complex (2004)
Grüber, Gerhard, Boekema, Egbert J., Oostergetel, Gert T., Keegstra, Wilko, Coskun, Ünal, Chaban, Yuriy L.
Co-reconstitution of subunits E and G of the yeast V-ATPase and the α and β subunits of the F1-ATPase from the thermophilic Bacillus PS3 (TF1) resulted in an α3β3EG hybrid complex showing 53% of...
Grüber, Gerhard, Boekema, Egbert J., Keegstra, Wilko, Müller, Volker, Lingl, Astrid, Chaban, Yuriy L., ...
In Archaea, bacteria, and eukarya, ATP provides metabolic energy for energy-dependent processes. It is synthesized by enzymes known as A-type or F-type ATP synthase, which are the smallest rotatory...
Ruban, Alexander V., Jansson, Stefan, Andersson, Jenny, Dekker, Jan P., Boekema, Egbert J., Keegstra, Wilko, ...
A genetic approach has been adopted to investigate the organization of the light-harvesting proteins in the photosystem II (PSII) complex in plants. PSII membrane fragments were prepared from...
Dekker, Jan P., Matthijs, Hans C.P., Keegstra, Wilko, Yakushevska, Alevtyna E., Haene, Sandrine D’, Yeremenko, Nataliya, ...
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of Photosystem I (PSI) and the chlorophyll-binding protein IsiA from a mutant...
Boekema, Egbert J., Lolkema, Juke S.
The ntpK gene of the archaeon Methanopyrus kandleri encodes the equivalent of the c subunit of ATP synthase. The gene product contains 1021 residues and consists of 13 homologous domains, each one...
Subunit Composition, Structure, and Distribution of Bacterial V-Type ATPases (2003)
Boekema, Egbert J., Chaban, Yuriy, Lolkema, Juke S.
The overall structure of V-ATPase complexes resembles that of F-type ATPases, but the stalk region is different and more complex. Database searches followed by sequence analysis of the five...
Ruban, Alexander V., Jansson, Stefan, Andersson, Jenny, Dekker, Jan P., Boekema, Egbert J., Keegstra, Wilko, ...
A genetic approach has been adopted to investigate the organization of the light-harvesting proteins in the photosystem II (PSII) complex in plants. PSII membrane fragments were prepared from...
Yakushevska, Alevtyna E., Haene, Sandrine D’, Yeremenko, Nataliya, Boekema, Egbert J., Dekker, Jan P., Matthijs, Hans C.P., ...
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of Photosystem I (PSI) and the chlorophyll-binding protein IsiA from a mutant...
Boekema, Egbert J., Lolkema, Juke S.
The ntpK gene of the archaeon Methanopyrus kandleri encodes the equivalent of the c subunit of ATP synthase. The gene product contains 1021 residues and consists of 13 homologous domains, each one...
Subunit Composition, Structure, and Distribution of Bacterial V-Type ATPases (2003)
Chaban, Yuriy, Lolkema, Juke S., Boekema, Egbert J.
The overall structure of V-ATPase complexes resembles that of F-type ATPases, but the stalk region is different and more complex. Database searches followed by sequence analysis of the five...
Composition of the central stalk of the Na+-pumping V-ATPase from Caloramator fervidus (2002)
Lolkema, Juke S., Keegstra, Wilko, Ubbink-Kok, Trees, Chaban, Yuriy, Boekema, Egbert J.
The Na+-pumping V-ATPase complex of the thermophilic bacterium Caloramator fervidus was purified and dissociated under controlled conditions. The structure of purified V1-ATPase subcomplexes...
Dekker, Jan P., Gorkom, Hans J. Van, Keegstra, Wilko, Yakushevska, Alevtyna E., Germano, Marta, Boekema, Egbert J.
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of photosystem I and light-harvesting complex I from the unicellular green...
Roon, Henny Van, Germano, Marta, Dekker, Jan P., Boekema, Egbert J.
We studied the aggregation state of Photosystem II in stacked and unstacked thylakoid membranes from spinach after a quick andmild solubilization with the non-ionic detergent...
Composition of the central stalk of the Na+-pumping V-ATPase from Caloramator fervidus (2002)
Boekema, Egbert J., Lolkema, Juke S., Keegstra, Wilko, Ubbink-Kok, Trees, Chaban, Yuriy
The Na+-pumping V-ATPase complex of the thermophilic bacterium Caloramator fervidus was purified and dissociated under controlled conditions. The structure of purified V1-ATPase subcomplexes...
Dekker, Jan P., Gorkom, Hans J. Van, Keegstra, Wilko, Yakushevska, Alevtyna E., Germano, Marta, Boekema, Egbert J.
We report a structural characterization by electron microscopy and image analysis of a supramolecular complex consisting of photosystem I and light-harvesting complex I from the unicellular green...
Boekema, Egbert J., Roon, Henny Van, Germano, Marta, Dekker, Jan P.
We studied the aggregation state of Photosystem II in stacked and unstacked thylakoid membranes from spinach after a quick andmild solubilization with the non-ionic detergent...
Green Plant Photosystem I Binds Light-Harvesting Complex I on One Side of the Complex (2001)
Dekker, Jan P., Scheller, Henrik Vibe, Roon, Henny Van, Schlodder, Eberhard, Jensen, Poul Erik, ...
We report a structural characterization by electron microscopy of green plant photosystem I solubilized by the mild detergent n-dodecyl-R-D-maltoside. It is shown by immunoblotting that the isolated...
Boekema, Egbert J., Scheller, Henrik V., Roon, Henny Van, Keegstra, Wilko, Jensen, Poul E., Yakushevska, Alevtyna E., ...
The organization of Arabidopsis thaliana photosystem II (PSII) and its associated light-harvesting antenna (LHCII) was studied in isolated PSII–LHCII supercomplexes and native membrane-bound...
Green Plant Photosystem I Binds Light-Harvesting Complex I on One Side of the Complex (2001)
Schlodder, Eberhard, Jensen, Poul Erik, Boekema, Egbert J., Dekker, Jan P., Scheller, Henrik Vibe, Roon, Henny Van, ...
We report a structural characterization by electron microscopy of green plant photosystem I solubilized by the mild detergent n-dodecyl-R-D-maltoside. It is shown by immunoblotting that the isolated...
Boekema, Egbert J., Scheller, Henrik V., Roon, Henny Van, Keegstra, Wilko, Jensen, Poul E., Yakushevska, Alevtyna E., ...
The organization of Arabidopsis thaliana photosystem II (PSII) and its associated light-harvesting antenna (LHCII) was studied in isolated PSII–LHCII supercomplexes and native membrane-bound...
Conformational Changes in Photosystem II Supercomplexes upon Removal of Extrinsic Subunits (2000)
Roon, Henny Van, Boekema, Egbert J., Dekker, Jan P.
Photosystem II is a multisubunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts. It consists of a large number of intrinsic membrane proteins involved in light-harvesting...
Roon, Henny Van, Boekema, Egbert J., Dekker, Jan P.
The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in...
Lolkema, Juke S., Konings, Wil N., Brisson, Alain, Boekema, Egbert J., Ubbink-Kok, Trees
The V-type Na+-ATPase of the thermophilic, anaerobic bacterium Caloramator fervidus was purified to homogeneity. The subunit compositions of the catalytic V1 and membrane-embedded V0 parts were...
Boekema, Egbert J., Dekker, Jan P., Weerd, Frank L. De, Roon, Henny Van
A biochemical and structural analysis is presented of fractions that were obtained by a quick and mild solubilization of thylakoid membranes from spinach with the non-ionic detergent...
Conformational Changes in Photosystem II Supercomplexes upon Removal of Extrinsic Subunits (2000)
Dekker, Jan P., Roon, Henny Van, Boekema, Egbert J.
Photosystem II is a multisubunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts. It consists of a large number of intrinsic membrane proteins involved in light-harvesting...
Dekker, Jan P., Roon, Henny Van, Boekema, Egbert J.
The chloroplast thylakoid membrane of green plants is organized in stacked grana membranes and unstacked stroma membranes. We investigated the structural organization of Photosystem II (PSII) in...
Brisson, Alain, Boekema, Egbert J., Lolkema, Juke S., Konings, Wil N., Ubbink-Kok, Trees
The V-type Na+-ATPase of the thermophilic, anaerobic bacterium Caloramator fervidus was purified to homogeneity. The subunit compositions of the catalytic V1 and membrane-embedded V0 parts were...
Dekker, Jan P., Weerd, Frank L. De, Roon, Henny Van, Boekema, Egbert J.
A biochemical and structural analysis is presented of fractions that were obtained by a quick and mild solubilization of thylakoid membranes from spinach with the non-ionic detergent...
Bassi, Roberto, Calkoen, Florentine, Roon, Henny Van, Boekema, Egbert J., Dekker, Jan P.
Photosystem II is a multisubunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts. It utilizes light for photochemical energy conversion, and is heavily involved in the...
Dekker, Jan P., Roon, Henny Van, Boekema, Egbert J.
We present an extended analysis of the organization of green plant photosystem II and its associated light-harvesting antenna using electron microscopy and image analysis. The analysis is based on a...
Boekema, Egbert J., Rögner, Matthias, Kuhl, Helena
A large set of electron microscopy projections of photosystem II (PSII) dimers isolated from the cyanobacterium Synechococcus elongatus was characterized by single particle image analysis. In...
Calkoen, Florentine, Roon, Henny Van, Boekema, Egbert J., Dekker, Jan P., Bassi, Roberto
Photosystem II is a multisubunit pigment-protein complex embedded in the thylakoid membranes of chloroplasts. It utilizes light for photochemical energy conversion, and is heavily involved in the...
Roon, Henny Van, Dekker, Jan P., Boekema, Egbert J.
We present an extended analysis of the organization of green plant photosystem II and its associated light-harvesting antenna using electron microscopy and image analysis. The analysis is based on a...
Rögner, Matthias, Kuhl, Helena, Boekema, Egbert J.
A large set of electron microscopy projections of photosystem II (PSII) dimers isolated from the cyanobacterium Synechococcus elongatus was characterized by single particle image analysis. In...
Hankamer, Ben, Nield, Jon, Boekema, Egbert J., Barber, James
A dimeric photosystem II light-harvesting II super complex (PSII-LHCII SC), isolated by sucrose density gradient centrifugation, was previously structurally characterized. This PSII-LHCII SC bound...
Dekker, Jan P., Roon, Henny Van, Boekema, Egbert J.
In this study, we report the structural characterization of photosystem II complexes obtained from partially solubilized photosystem II membranes. Direct observation by electron microscopy, within a...
Barber, James, Hankamer, Ben, Nield, Jon, Boekema, Egbert J.
A dimeric photosystem II light-harvesting II super complex (PSII-LHCII SC), isolated by sucrose density gradient centrifugation, was previously structurally characterized. This PSII-LHCII SC bound...
Roon, Henny Van, Dekker, Jan P., Boekema, Egbert J.
In this study, we report the structural characterization of photosystem II complexes obtained from partially solubilized photosystem II membranes. Direct observation by electron microscopy, within a...
Structure and Membrane Organization of Photosystem II in Green Plants (1997)
Barber, James, Hankamer, Ben, Boekema, Egbert J.
Photosystem II (PSII) is the pigment protein complex embedded in the thylakoid membrane of higher plants, algae, and cyanobacteria that uses solar energy to drive the photosynthetic water-splitting...
Boekema, Egbert J., Dekker, Jan P., Eijckelhoff, Camiel
Dimeric associations of the D1-D2-CP47 and D1-D2-CP47-CP43 complexes of Photosystem II from spinach were isolated and purified with sucrose density gradient centrifugation and gel filtration...
Rögner, Matthias, Lagoutte, Bernard, Chitnis, Parag R., Kruip, Jochen, Boekema, Egbert J.
Based on an improved isolation procedure using perfusion chromatography, trimeric Photosystem 1 (PS1) complexes have been isolated from various deletion mutants of the mesophilic cyanobacterium...
Structure and Membrane Organization of Photosystem II in Green Plants (1997)
Boekema, Egbert J., Barber, James, Hankamer, Ben
Photosystem II (PSII) is the pigment protein complex embedded in the thylakoid membrane of higher plants, algae, and cyanobacteria that uses solar energy to drive the photosynthetic water-splitting...
Boekema, Egbert J., Dekker, Jan P., Eijckelhoff, Camiel
Dimeric associations of the D1-D2-CP47 and D1-D2-CP47-CP43 complexes of Photosystem II from spinach were isolated and purified with sucrose density gradient centrifugation and gel filtration...
Rögner, Matthias, Lagoutte, Bernard, Chitnis, Parag R., Kruip, Jochen, Boekema, Egbert J.
Based on an improved isolation procedure using perfusion chromatography, trimeric Photosystem 1 (PS1) complexes have been isolated from various deletion mutants of the mesophilic cyanobacterium...
Brisson, Alain, Keegstra, Wilko, Grondelle, Rienk Van, Visschers, Ronald, Ortiz De Zarate, Inaki, Boekema, Egbert J., ...
Two-dimensional crystals of LH2 (B800-850) light-harvesting complexes from Ectothiorhodospira sp. and Rhodobacter capsulatus were obtained by reconstitution of purified protein into phospholipid...
Sétif, Pierre, Rögner, Matthias, Bottin, Hervé, Kruip, Jochen, Boekema, Egbert J., Lelong, Cécile
A covalent stoichiometric complex between photosystem I (PSI) and ferredoxin from the cyanobacterium Synechocystis sp. PCC 6803 was generated by chemical cross-linking. The photoreduction of...
How does photosystem 2 split water? The structural basis of efficient energy conversion (1996)
Boekema, Egbert J., Rögner, Matthias, Barber, Jim
Photosystem 2 (PS2) is the part of the photosynthetic apparatus that uses light energy to split water releasing oxygen, protons and electrons. Here, we present a model of the subunit organization of...
Boekema, Egbert J., Rögner, Matthias
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very...
The Structure of the CF1 Part of the ATP-Synthase Complex from Chloroplasts (1996)
Boekema, Egbert J., Lücken, Uwe
The proton ATP synthase consists of a membrane integrated part, Fo, and a hydrophilic part, F1. F1 is composed of five different subunits: α, β, γ, δ and ε. This chapter focuses on the...
Ortiz De Zarate, Inaki, Boekema, Egbert J., Oling, Frank, Visschers, Ronald, Grondelle, Rienk Van, Keegstra, Wilko, ...
Two-dimensional crystals of LH2 (B800-850) light-harvesting complexes from Ectothiorhodospira sp. and Rhodobacter capsulatus were obtained by reconstitution of purified protein into phospholipid...
Rögner, Matthias, Boekema, Egbert J.
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very...
The Structure of the CF1 Part of the ATP-Synthase Complex from Chloroplasts (1996)
Lücken, Uwe, Boekema, Egbert J.
The proton ATP synthase consists of a membrane integrated part, Fo, and a hydrophilic part, F1. F1 is composed of five different subunits: α, β, γ, δ and ε. This chapter focuses on the...
How does photosystem 2 split water? The structural basis of efficient energy conversion (1996)
Barber, Jim, Boekema, Egbert J., Rögner, Matthias
Photosystem 2 (PS2) is the part of the photosynthetic apparatus that uses light energy to split water releasing oxygen, protons and electrons. Here, we present a model of the subunit organization of...
Sétif, Pierre, Rögner, Matthias, Bottin, Hervé, Kruip, Jochen, Boekema, Egbert J., Lelong, Cécile
A covalent stoichiometric complex between photosystem I (PSI) and ferredoxin from the cyanobacterium Synechocystis sp. PCC 6803 was generated by chemical cross-linking. The photoreduction of...
Boekema, Egbert J., Nienhuis-Kuiper, Manny E., Jansen, Michael, Hensgens, Charles M.H., Hansen, Theo A.
The sulfate-reducing bacterium Desulfovibrio strain HDv (DSM 6830) grew faster on (S)- and on (R, S)-1,2-propanediol (µmax 0.053 h–1) than on (R)-propanediol (0.017 h–1) and ethanol (0.027...
Electron cryomicroscopy of two-dimensional crystals of the H+-ATPase from chloroplasts (1995)
Lücken, Uwe, Boekema, Egbert J., Gräber, Peter, Böttcher, Bettina
The H+-ATPase from spinach chloroplasts was isolated and purified. Two-dimensional crystals were obtained from the protein/lipid/detergent micelles by treatment with phospholipase and simultaneous...
Supramolecular structure of the photosystem II complex from green plants and cyanobacteria (1995)
Rögner, Matthias, Barber, James, Boonstra, Arjen F., Nield, Jon, Kruip, Jochen, Bald, Dirk, ...
Photosystem II (PSII) complexes, isolated from spinach and the thermophilic cyanobacterium Synechococcus elongatus, were characterized by electron microscopy and single-particle image-averaging...
Jansen, Michael, Hensgens, Charles M.H., Nienhuis-Kuiper, Manny E., Boekema, Egbert J., Hansen, Theo A.
The sulfate-reducing bacterium Desulfovibrio strain HDv (DSM 6830) grew faster on (S)- and on (R, S)-1,2-propanediol (µmax 0.053 h–1) than on (R)-propanediol (0.017 h–1) and ethanol (0.027...
Electron cryomicroscopy of two-dimensional crystals of the H+-ATPase from chloroplasts (1995)
Lücken, Uwe, Boekema, Egbert J., Gräber, Peter, Böttcher, Bettina
The H+-ATPase from spinach chloroplasts was isolated and purified. Two-dimensional crystals were obtained from the protein/lipid/detergent micelles by treatment with phospholipase and simultaneous...
Supramolecular structure of the photosystem II complex from green plants and cyanobacteria (1995)
Kruip, Jochen, Bald, Dirk, Hankamer, Ben, Boekema, Egbert J., Nield, Jon, Boonstra, Arjen F., ...
Photosystem II (PSII) complexes, isolated from spinach and the thermophilic cyanobacterium Synechococcus elongatus, were characterized by electron microscopy and single-particle image-averaging...
Boekema, Egbert J., Germeroth, Lothar, Boonstra, Arjen F.
The structure of two types of isolated light-harvesting antenna complexes from Rhodospirillum molischianum was studied by electron microscopy and image analysis. The B870 reaction center complex...
Rögner, Matthias, Dekker, Jan P., Boonstra, Arjen F., Boekema, Egbert J.
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very...
Boekema, Egbert J., Germeroth, Lothar, Boonstra, Arjen F.
The structure of two types of isolated light-harvesting antenna complexes from Rhodospirillum molischianum was studied by electron microscopy and image analysis. The B870 reaction center complex...
Rögner, Matthias, Dekker, Jan P., Boonstra, Arjen F., Boekema, Egbert J.
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structure at low- and high resolution. Since electron micrographs of biological objects are very...
Grondelle, Rienk Van, Calkoen, Florentine, Visschers, Ronald W., Boonstra, Arjen F., Boekema, Egbert J.
The structure and aggregation behavior of B800-850 (LHII) and B875 (LHI) antenna complexes of Rhodobacter sphaeroides were studied by electron microscopy. Single molecular projections (top views and...
Matthijs, Hans C.P., Dekker, Jan P., Boekema, Egbert J.
Photosystem I particles from Prochlorothrix hollandica were isolated from dodecylmaltoside solubilized thylakoid membranes on sucrose gradients in the presence of dodecylmaltoside. The particles were...
Boonstra, Arjen F., Bald, Dirk, Boekema, Egbert J., Kruip, Jochen, Rögner, Matthias
An isolation procedure was developed for the cyanobacterium Synechocystis 6803 (and 6714) which yields both monomeric and trimeric photosystem I complexes (P700·FA/FB complexes) depleted of the...
Keegstra, Wilko, Boekema, Egbert J., Haas, Felix De
Structural differences between oxy-hemocyanin and deoxy-hemocyanin from the spiny lobster P. interruptus were studied by electron microscopy and image analysis of negatively stained preparations....
Calkoen, Florentine, Visschers, Ronald W., Boonstra, Arjen F., Grondelle, Rienk Van, Boekema, Egbert J.
The structure and aggregation behavior of B800-850 (LHII) and B875 (LHI) antenna complexes of Rhodobacter sphaeroides were studied by electron microscopy. Single molecular projections (top views and...
Matthijs, Hans C.P., Dekker, Jan P., Boekema, Egbert J.
Photosystem I particles from Prochlorothrix hollandica were isolated from dodecylmaltoside solubilized thylakoid membranes on sucrose gradients in the presence of dodecylmaltoside. The particles were...
Rögner, Matthias, Boonstra, Arjen F., Bald, Dirk, Boekema, Egbert J., Kruip, Jochen
An isolation procedure was developed for the cyanobacterium Synechocystis 6803 (and 6714) which yields both monomeric and trimeric photosystem I complexes (P700·FA/FB complexes) depleted of the...
Boekema, Egbert J., Haas, Felix De, Keegstra, Wilko
Structural differences between oxy-hemocyanin and deoxy-hemocyanin from the spiny lobster P. interruptus were studied by electron microscopy and image analysis of negatively stained preparations....
Boekema, Egbert J., Böttcher, Bettina
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been studied by electron microscopy and image analysis of negatively stained samples. The projections of...
Boekema, Egbert J., Gräber, Peter, Böttcher, Bettina
The structure of the Photosystem I (PS I) complex from the thermophilic cyanobacterium Synechococcus sp. has been investigated by electron microscopy and image analysis of two-dimensional crystals....
Tagunova, Irina V., Pushkin, Alexander V., Boekema, Egbert J., Tsuprun, Vladimir L.
The molecular structure of groEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected...
Böttcher, Bettina, Boekema, Egbert J.
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been studied by electron microscopy and image analysis of negatively stained samples. The projections of...
Boekema, Egbert J., Gräber, Peter, Böttcher, Bettina
The structure of the Photosystem I (PS I) complex from the thermophilic cyanobacterium Synechococcus sp. has been investigated by electron microscopy and image analysis of two-dimensional crystals....
Pushkin, Alexander V., Boekema, Egbert J., Tsuprun, Vladimir L., Tagunova, Irina V.
The molecular structure of groEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected...
Negative Staining of Integral Membrane Proteins (1991)
This review describes aspects of negative staining of isolated integral membrane proteins. Detergents play a central role in the isolation of membrane proteins and also in their solubility in aqueous...
Negative Staining of Integral Membrane Proteins (1991)
This review describes aspects of negative staining of isolated integral membrane proteins. Detergents play a central role in the isolation of membrane proteins and also in their solubility in aqueous...
Boekema, Egbert J., Yocum, Charles F., Betts, Scott D., Dekker, Jan P.
A photosystem II complex containing the reaction center proteins D1 and D2, a 47-kDa chlorophyll-binding protein (CP47), and cytochrome b-559 was isolated with high yield, purity, and homogeneity;...
The structure of spinach Photosystem I studied by electron microscopy (1990)
Malkin, Richard, Wynn, R. Max, Boekema, Egbert J.
The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and...
McCarty, Richard E., Xiao, Jianping, Boekema, Egbert J.
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been further investigated by electron microscopy and image analysis of negatively stained samples. The...
The Present State of Two-Dimensional Crystallization of Membrane Proteins (1990)
This review summarizes the present literature on two-dimensional crystallization of membrane proteins, with emphasis on the technical aspects. It includes all the intrinsic membrane proteins that...
Renger, Gernot, Boekema, Egbert J., Irrgang, Klaus-D., Haag, Elisabeth
Oxygen-evolving photo system II core complexes were prepared from spinach by solubilizing photosystem II membrane fragments with dodecyl-β-D-maltoside. The core complexes consist of the intrinsic...
Fuhrhop, Juergen-H., Boekema, Egbert J., Boettcher, Christoph
The combined application of autoradiography and electron microscopy allowed the assignment of molecular components to individual micellar fibres in a mixed gel. Resolution was of the order of 0·1...
Yocum, Charles F., Betts, Scott D., Dekker, Jan P., Boekema, Egbert J.
A photosystem II complex containing the reaction center proteins D1 and D2, a 47-kDa chlorophyll-binding protein (CP47), and cytochrome b-559 was isolated with high yield, purity, and homogeneity;...
The structure of spinach Photosystem I studied by electron microscopy (1990)
Malkin, Richard, Wynn, R. Max, Boekema, Egbert J.
The structure of three types of Photosystem I (PS I) complex isolated from spinach chloroplasts was studied by electron microscopy and computer image analysis. Molecular projections (top views and...
McCarty, Richard E., Xiao, Jianping, Boekema, Egbert J.
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been further investigated by electron microscopy and image analysis of negatively stained samples. The...
The Present State of Two-Dimensional Crystallization of Membrane Proteins (1990)
This review summarizes the present literature on two-dimensional crystallization of membrane proteins, with emphasis on the technical aspects. It includes all the intrinsic membrane proteins that...
Boekema, Egbert J., Irrgang, Klaus-D., Haag, Elisabeth, Renger, Gernot
Oxygen-evolving photo system II core complexes were prepared from spinach by solubilizing photosystem II membrane fragments with dodecyl-β-D-maltoside. The core complexes consist of the intrinsic...
Fuhrhop, Juergen-H., Boekema, Egbert J., Boettcher, Christoph
The combined application of autoradiography and electron microscopy allowed the assignment of molecular components to individual micellar fibres in a mixed gel. Resolution was of the order of 0·1...
Heel, Marin Van, Boekema, Egbert J.
The molecular structure of erythrocruorin (hemoglobin) from Lumbricus terrestris has been studied by electron microscopy of negatively stained particles. Over 1000 molecular projections were selected...
Heel, Marin Van, Boekema, Egbert J.
The molecular structure of erythrocruorin (hemoglobin) from Lumbricus terrestris has been studied by electron microscopy of negatively stained particles. Over 1000 molecular projections were selected...
On the Structure of the ATP-Synthase from Chloroplasts (1988)
Gräber, Peter, Fromme, Petra, Boekema, Egbert J.
The H+-translocating ATPase ("ATP-synthase, CF0F1) was isolated from chloroplasts and characterized biochemically and by high resolution electron microscopy. The ATP-synthase has a hydrophilic part,...
Gräber, Peter, Heel, Marin Van, Boekema, Egbert J.
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been investigated by electron microscopy of negatively stained samples. The staining conditions, which are...
Structural determination of the photosystem II core complex from spinach (1988)
Vater, Joachim, Irrgang, Klaus-D., Boekema, Egbert J., Renger, Gernot
A photosystem II core complex was purified with high yield from spinach by solubilization with β-dodecylmaltoside. The complex consisted of polypeptides with molecular mass 47, 43, 34, 31, 9 and 4...
On the Structure of the ATP-Synthase from Chloroplasts (1988)
Fromme, Petra, Boekema, Egbert J., Gräber, Peter
The H+-translocating ATPase ("ATP-synthase, CF0F1) was isolated from chloroplasts and characterized biochemically and by high resolution electron microscopy. The ATP-synthase has a hydrophilic part,...
Gräber, Peter, Heel, Marin Van, Boekema, Egbert J.
The structure of the hydrophilic part of the ATP synthase from chloroplasts (CF1) has been investigated by electron microscopy of negatively stained samples. The staining conditions, which are...
Structural determination of the photosystem II core complex from spinach (1988)
Boekema, Egbert J., Irrgang, Klaus-D., Vater, Joachim, Renger, Gernot
A photosystem II core complex was purified with high yield from spinach by solubilization with β-dodecylmaltoside. The complex consisted of polypeptides with molecular mass 47, 43, 34, 31, 9 and 4...
Boekema, Egbert J., Cleeter, Mike W.J., Ragan, C. Ian, Hovmöller, Sven, Brink, Jaap
We have investigated the structure of two-dimensional crystals from preparations of NADH:ubiquinone oxidoreductase from beef-heart mitochondria. The crystal structure of these crystals was previously...
Ragan, C. Ian, Hovmöller, Sven, Brink, Jaap, Cleeter, Mike W.J., Boekema, Egbert J.
We have investigated the structure of two-dimensional crystals from preparations of NADH:ubiquinone oxidoreductase from beef-heart mitochondria. The crystal structure of these crystals was previously...
Structure of mitochondrial F1-ATPase studied by electron microscopy and image processing (1986)
Heel, Marin G. Van, Berden, Jan A., Boekema, Egbert J.
The structure of soluble F1-ATPase (EC 3.6.1.3) has been investigated by computer analysis of individual molecular images extracted from electron micrographs of negatively stained particles. A total...
Structure of mitochondrial F1-ATPase studied by electron microscopy and image processing (1986)
Heel, Marin G. Van, Berden, Jan A., Boekema, Egbert J.
The structure of soluble F1-ATPase (EC 3.6.1.3) has been investigated by computer analysis of individual molecular images extracted from electron micrographs of negatively stained particles. A total...
Heel, Marin G. Van, Boekema, Egbert J.
We have studied the structure of bovine heart mitochondrial NADH:ubiquinone (Q) oxidoreductase (EC 1.6.99.3) by image analysis of electron micrographs. A three-dimensional reconstruction was...
Heel, Marin G. Van, Boekema, Egbert J.
We have studied the structure of bovine heart mitochondrial NADH:ubiquinone (Q) oxidoreductase (EC 1.6.99.3) by image analysis of electron micrographs. A three-dimensional reconstruction was...
Boekema, Egbert J., Keegstra, Wilko, Albracht, Simon P.J.
Two-dimensional crystalline arrays of NADH:Q oxidoreductase preparations have been obtained by microdiffusion of protein dissolved in detergent against a 15 mM sodium acetate buffer of pH 5.5...
Boekema, Egbert J., Keegstra, Wilko, Albracht, Simon P.J.
Two-dimensional crystalline arrays of NADH:Q oxidoreductase preparations have been obtained by microdiffusion of protein dissolved in detergent against a 15 mM sodium acetate buffer of pH 5.5...
Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III
Dudkina, Natalia V., Eubel, Holger, Keegstra, Wilko, Boekema, Egbert J., Braun, Hans-Peter
Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes:...
Composition of the central stalk of the Na+-pumping V-ATPase from Caloramator fervidus
Chaban, Yuriy, Ubbink-Kok, Trees, Keegstra, Wilko, Lolkema, Juke S., Boekema, Egbert J.
The Na+-pumping V-ATPase complex of the thermophilic bacterium Caloramator fervidus was purified and dissociated under controlled conditions. The structure of purified V1-ATPase subcomplexes...
Structure of a mitochondrial supercomplex formed by respiratory-chain complexes I and III
Dudkina, Natalia V., Eubel, Holger, Keegstra, Wilko, Boekema, Egbert J., Braun, Hans-Peter
Mitochondria are central to the efficient provision of energy for eukaryotic cells. The oxidative-phosphorylation system of mitochondria consists of a series of five major membrane complexes:...
Composition of the central stalk of the Na+-pumping V-ATPase from Caloramator fervidus
Chaban, Yuriy, Ubbink-Kok, Trees, Keegstra, Wilko, Lolkema, Juke S., Boekema, Egbert J.
The Na+-pumping V-ATPase complex of the thermophilic bacterium Caloramator fervidus was purified and dissociated under controlled conditions. The structure of purified V1-ATPase subcomplexes...
Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus▿
Szabó, Zalán, Sani, Musa, Groeneveld, Maarten, Zolghadr, Benham, Schelert, James, Albers, Sonja-Verena, ...
Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed...
Kovács, László, Damkjær, Jakob, Kereïche, Sami, Ilioaia, Cristian, Ruban, Alexander V., Boekema, Egbert J., ...
The photosystem II (PSII) light-harvesting antenna in higher plants contains a number of highly conserved gene products whose function is unknown. Arabidopsis thaliana plants depleted of one of...
Structure and organization of phycobilisomes on membranes of the red alga Porphyridium cruentum
Arteni, Ana A., Liu, Lu-Ning, Aartsma, Thijs J., Zhang, Yu-Zhong, Zhou, Bai-Cheng, Boekema, Egbert J.
In the present work, electron microscopy and single particle averaging was performed to investigate the supramolecular architecture of hemiellipsoidal phycobilisomes from the unicellular red alga...
Alternating syn-anti bacteriochlorophylls form concentric helical nanotubes in chlorosomes
Ganapathy, Swapna, Oostergetel, Gert T., Wawrzyniak, Piotr K., Reus, Michael, Gomez Maqueo Chew, Aline, Buda, Francesco, ...
Chlorosomes are the largest and most efficient light-harvesting antennae found in nature, and they are constructed from hundreds of thousands of self-assembled bacteriochlorophyll (BChl) c, d, or e...
Single particle electron microscopy
Boekema, Egbert J., Folea, Mihaela, Kouřil, Roman
Electron microscopy (EM) in combination with image analysis is a powerful technique to study protein structures at low, medium, and high resolution. Since electron micrographs of biological objects...
Dudkina, Natalya V., Sunderhaus, Stephanie, Boekema, Egbert J., Braun, Hans-Peter
The organization of the oxidative phosphorylation (OXPHOS) system within the inner mitochondrial membrane appears to be far more complicated than previously thought. In particular, the individual...