Development of novel chemical probes to detect abasic sites in DNA (2009)
Kojima, Naoshi, Takebayashi, Toshie, Mikami, Akiko, Ohtsuka, Eiko, Komatsu, Yasuo
We chemically synthesized a series of aminooxy derivatives to develop novel probes for sensitive detection of abasic (AP) sites in DNA. The results of the conjugation reactions showed that the probes...
Construction of an aminooxy derivative for RNA and DNA labeling (2008)
Komatsu, Yasuo, Kojima, Naoshi, Takebayashi, Toshie, Mikami, Akiko, Sugino, Maiko, Ohtsuka, Eiko
We previously reported that the reactivity of a primary amine is increased by connecting the amine to an aromatic residue with an alkyl linker. This strategy was applied to the construction of a...
Kojima, Naoshi, Takebayashi, Toshie, Mikami, Akiko, Sugino, Maiko, Ohtsuka, Eiko, Komatsu, Yasuo
We previously reported a series of new amino-linkers, consisting of an aminoethyl carbamate structure (Komatsu, 2008). We have now examined the chemical properties of oligonucleotides modified with...
Kojima, Naoshi, Sugino, Maiko, Mikami, Akiko, Nonaka, Ken, Fujinawa, Yumi, Ueda, Yoji, ...
We report here a new amino-modifier reagent, which enable high-throughput purification of amino-modified oligonucleotides. Either monomethoxytrityl (MMT) or trifluoroacetyl (TFA) group has been used...
Synthesis and application of new amino modification analogues for functional oligonucleotide (2004)
Komatsu, Yasuo, Kojima, Naoshi, Nonaka, Ken, Fujinawa, Yumi, Sugino, Maiko, Mikami, Akiko, ...
We synthesized new analogues to functionalize oligonucleotides. These analogues included a primary amino group tethering to an aromatic ring, and we introduced them into the 5′-end of each...
Stable hairpins having a loop consisting of 3'-deoxy-4'-C-(2-hydroxyethyl)thymidines (2004)
Shuto, Satoshi, Tamura, Yutaka, Yamamoto, Yuji, Kodama, Tetsuya, Hoshika, Shuichi, Ichikawa, Satoshi, ...
A novel sugar-modified thymidine analogue, 3′-deoxy-4′-C-(2-hydroxyethyl)thymidine (X), was designed and synthesized to show that it can stabilize hairpin structures when it is present in the...
Sakamoto, Satoshi, Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Kitamura, Masaya, ...
Based on our recent studies of RNA cleavage by oligonucleotide–terpyridine·Cu(II) complex 5′‐ and/or 3′‐conjugates, we designed 2′‐O‐methyloligonucleotides with two...
Kojima, Naoshi, Inoue, Kaori, Nakajima-Shibata, Rina, Kawahara, Shun-ichi, Ohtsuka, Eiko
The first synthesis of 5‐amino‐3‐(2′‐deoxy‐β‐d‐ribofuranosyl)imidazo[4,5‐b]pyridin‐7‐one (1‐deaza‐2′‐deoxyguanosine) is...
Sakamoto, Satoshi, Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Kitamura, Masaya, ...
Sakamoto, Satoshi, Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Kitamura, Masaya, ...
We synthesized a 2′-O-methyloligonucleotide with two terpyridine-Cu(II) complexes in the central part, and found that this antisense agent cleaves an RNA oligomer more efficiently than an analogous...
Yoshiharu Iida, Osamu Inanami, Hideo Inoue, Eiko Ohtsuka, Mikinori Kuwabara
Iida, Y., Inanami, O., Inoue, H., Ohtsuka, E. and Kuwabara, M. Unique Response of Double-Stranded Oligonucleotides Containing a Single 8-Oxo-7,8-Dihydroguanine to Gamma Rays in the Frozen Aqueous...
Inoue, Hideo, Furukawa, Takako, Tamura, Takashi, Komatsu, Yasuo, Ohtsuka, Eiko
In an approach toward artificial ribbnucleases,novel RNA cleaving systems were constructed that contained two terpyridine·Cu(II) residues. The first antisense system used tandem Cu(II)...
Torizawa, Takuya, Yamamoto, Nobuhiro, Suzuki, Tomonori, Nobuoka, Kaoru, Komatsu, Yasuo, Morioka, Hiroshi, ...
Monoclonal antibodies specific for the cyclobutane pyrimidine dimer (CPD) are widely used for detection and quantification of DNA photolesions. However, the mechanisms of antigen binding by anti-CPD...
Ling, Feng, Morioka, Hiroshi, Ohtsuka, Eiko, Shibata, Takehiko
A nuclear recessive mutant in Saccharomyces cerevisiae, mhr1-1, is defective in mitochondrial genetic recombination at 30°C and shows extensive vegetative petite induction by UV irradiation at 30°C...
Functional Domain-Assembly in Hairpin Ribozymes (2000)
Komatsu, Yasuo, Kanzaki, Ikuyo, Shirai, Miho, Kumagai, Izumi, Yamashita, Shigeko, Ohtsuka, Eiko
Functional structures of hairpin ribozymes have been investigated by constructing various chemically modified molecules. Domain-exchange and linker insertion experiments were performed to find active...
Kobayashi, Hiroyuki, Kato, Jiro, Morioka, Hiroshi, Stewart, Jon D., Ohtsuka, Eiko
The importance of Trp H33 in antibody recognition of DNA containing a central pyrimidine (6–4) pyrimidone photoproduct was investigated. This residue was replaced by Tyr, Phe and Ala and the...
Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Inoue, Hideo
In order to construct an antisense 2′-0-methyl-oligonucleotide with an internal terpyridine-Cu(II) complex, a novel synthon was synthesized. This synthon was a...
Nucleic acid synthesis for investigations of gene functions (1999)
The chemical syntheses of genes encoding human c-Ha-ras and T4 endonuclease V are described. These genes have been used for studies of mutagenesis by damaged bases and recognition of nucleic acids by...
Crystal structures of the 64M-2 and 64M-3 antibody Fabs complexed with DNA (6-4)photoproducts (1999)
Yokoyama, Hideshi, Mizutani, Ryuta, Satow, Yoshinori, Komatsu, Yasuo, Ohtsuka, Eiko, Nikaido, Osamu
Crystal structures of the 64M-2 antibody Fab fragment complexed with DNA photoproducts of dT(6-4)T and dTT(6-4)TT, and of the 64M-3 Fab fragment complexed with dT(6-4)T were determined. The...
Induction of ribozyme activity by anti-ribozyme oligonucleotides (1999)
Komatsu, Yasuo, Yamashita, Shigeko, Kazama, Nobuhiro, Ohtsuka, Eiko
A new type of hammerhead ribozyme, with cleavage activity enhanced by oligonucleotides, was constructed. Stem II of the ribozyme was substituted with a non complementary loop (loop II). The modified...
Further studies of KMnO4 oxidation of synthetic DNAs containing oxidatively damaged bases (1999)
Terashima, Yuko, Fukuoka, Masayoshi, Ohtsuka, Eiko, Inoue, Hideo
Recently we found that KMnO4 oxidation of DNA oligomers containing a 7,8-dihydro-8-oxoguanine (8-oxo-G) residue induces damage to the neighboring base residues; other modified bases,...
Chemical synthesis and properties of (6-4) photoproduct and its analogs (1999)
Sato, Kousuke, Komatsu, Yasuo, Torizawa, Takuya, Kato, Koichi, Shimada, Ichio, Ohtsuka, Eiko
We report the preparation of a deoxyribooligonucleotide containing a new thymine (6-4) photoproduct analog. The (6-4) photoproduct is one of the major forms of DNA lesions, and leads to mutation in...
Kobayashi, Hiroyuki, Morioka, Hiroshi, Torizawa, Takuya, Kato, Koichi, Shimada, Ichio, Nikaido, Osamu, ...
Pyrimidine (6–4) pyrimidone photoproducts are some of the major DNA photolesions induced by ultraviolet (UV) light. A monoclonal antibody (64M5) specific to a (6–4) photoproduct has been...
Komatsu, Yasuo, Shirai, Miho, Ohtsuka, Eiko
The three-domain hairpin ribozyme was improved by increasing the number of linker bases connecting domain I' with domain II from six to seven, and a new ribozyme was designed to release a...
Masuda, Ken-ichi, Kamimura, Takashi, Kanesaki, Masaharu, Ishii, Koji, Imaizumi, Atsushi, Sugiyama, Takashi, ...
We have developed a high-level production system for the C-terminal domain of secretory leukoprotease inhibitor (SLPI) to investigate its pharmacological activities. A gene for the C-terminal domain...
Shimoi, Kayoko, Miyamura, Rieko, Mori, Toshio, Todo, Takesi, Ohtsuka, Eiko, Wakabayashi, Keiji, ...
3-Amino-1, 4-dimethyl-5H-pyrido[4, 3-b]indole (Trp-P-1) is a mutagen/carcinogen derived from cooked foods which enhances the induction of mutations and chromosome aberrations by UV without microsomal...
Tateishi, Satoshi, Mori, Seiichi, Sugano, Tatsuo, Hori, Naoko, Ohtsuka, Eiko, Yamaizumi, Masaru
We fractionated HeLa cell extracts by gel filtration and then micro-injected them into cells derived from the seven complementation groups (A-G) of xeroderma pigmentosum (XP). Distinct fractions that...
Structural Composition of Hammerhead Ribozymes (1995)
Takenaka, Akio, Matsumoto, Osamu, Chen, Yixin, Hasegawa, Sei-ichi, Chatake, Toshiyuki, Tsunoda, Masaru, ...
Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains...
Kamiya, Hiroyuki, Miura, Hiroyuki, Murata-Kamiya, Naoko, Ishikawa, Hiroyuki, Sakaguchi, Tomoki, Inoue, Hideo, ...
An oligodeoxyribonucleotide containing 8-hydroxyadenine (OH8Ade) was chemically synthesized and single- and double-stranded c-Ha-ras gene fragments with OH8Ade at the second position of codon 61 were...
Kamiya, Hiroyuki, Murata-Kamiya, Naoko, Koizume, Shiro, Inoue, Hideo, Nishimura, Susumu, Ohtsuka, Eiko
We previously reported that 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) at the second position of codon 12 of the c-Ha-ras gene induces many types of mutations in NIH3T3 cells. In this study we...
Role of the Mg2+Ion in the Escherichia coli Ribonuclease HI Reaction (1994)
Uchiyama, Yohtaro, Iwai, Shigenori, Ueno, Yoshio, Ikehara, Morio, Ohtsuka, Eiko
To study the interaction and the role of the metal ion in the reaction catalyzed by Escherichia coli ribonuclease HI (E. coli RNase HI), substrate analogues containing a phosphorothioate linkage or...
Cross-ligation and exchange reactions catalyzed by hairpin ribozymes (1993)
Komtsu, Yasuo, Koizumi, Makoto, Sekiguchi, Akira, Ohtsuka, Eiko
The negative strand of the satellite RNA of tobacco ringspot virus (sTobRV( -)) contains a hairpin catalytic domain that shows self-cleavage and serf-ligation activities in the presence of magnesium...
Kamiya, Hiroyuki, Murata, Naoko, Murata, Tetsuya, Iwai, Shigenori, Matsukage, Akio, Masutani, Chikahide, ...
ras proto-oncogenes with a cyclobutane-type thymine photodlmer (cls-syn or trans-syn Isomer) were constructed by replacement of a portion of the gene with a chemically synthesized fragment. When the...
Bae, Yong-Soo, Kang, Yup, Ohtsuka, Eiko, Yoon, Ji-Won
The murine cardioviruses and bovine aphthoviruses are distinguished from other (+) strand RNA viruses by their long poly(C) tract in the 5′-noncoding region. The presence of this poly(C) tract has...
Binding of nucleic acids to E.coli RNase HI observed by NMR and CD spectroscopy (1993)
Oda, Yasushi, Iwa, Shigenori, Ohtsuka, Eiko, Ishikawa, Momoyo, Ikehara, Morio, Nakamura, Haruki
To clarify the mechanism by which the RNA portion of a DNA/RNA hybrid is specifically hydrolyzed by ribonuclease H (RNase H), the binding of a DNA/RNA hybrid, a DNA/DNA duplex, or an RNA/RNA duplex...
Crystal Structure of RNase T1(Y45W) Complexed with 3' AMP and Gf1pA (1993)
Gohda, Keigo, Itoh, Takeshi, Hiramatsu, Yasuyuki, Tomita, Ken-ichi, Nishikawa, Satoshi, Uesugi, Sei-ichi, ...
We have previously reported the crystallization of a mutant RNase T1(Y46W) with a synthetic modified trinucleotide ApGflpA [Hakoshima, T. et al. (1990) J. Biochem 108, 695–698]. In the present...
Kamiya, Hiroyuki, Suzuki, Masayuki, Komatsu, Yasuo, Miura, Hiroyuki, Kikuchi, Kanae, Sakaguchi, Tomoki, ...
Synthetic c-Ha-ras genes with an analogue of an abasic site in the first or the second position of codon 12, or in the second position of codon 61 were constructed and transfected into NIH3T3 cells....
Hori, Naoko, Doi, Tomoko, Karaki, Yoko, Kituchi, Masakazu, Ikehara, Morio, Ohtsuka, Eiko
T4 endonuclease V catalyzes the hydrolysis of the glycosyl bond of a thymine dimer in a DNA duplex and the cleavage of the 3′-phosphate by β-elimination. We have previously identified a catalytic...
Kido, Kaori, Inoue, Hideo, Ohtsuka, Eiko
Antisense oligodeoxyribonucleotldes (15mers), containing a 2-(N-iodoacetylaminoethyl)thlo-adenine, were synthesized and tested for their ability to cleave complementary DNAs (21 mers). Cleavage of...
NMR studies of a DNA containing 8-hydroxydeoxyguanosine (1991)
Oda, Yasushi, Uesugi, Seichi, Ikehara, Morio, Nishimura, Susumu, Kawase, Yasutoshi, Ishikawa, Hiroyuki, ...
The effects of hydroxylation at the C8 of a deoxyguanosine residue in DNA were studied by NMR analysis of a self-complementary dodecanucleotide, d(C1-G2-C3-oh8G4-A5-A6-T7-T8-C9-G10-C11-G12), which...
NMR studies for identification of dl:dG mismatch base-pairing structure in DNA (1991)
Oda, Yasushi, Uesugi, Seiichi, Ikehara, Morio, Kawase, Yasutoshi, Ohtsuka, Eiko
One- and two-dimensional NMR experiments have been undertaken to Investigate deoxylnosine:deoxyguanosine (dl:dG) base pairing in a seff-complementary dodecadeoxyribonucleotide, d(C1...
Sekiguchi, Akira, Komatsu, Yasuo, Koizumi, Makoto, Ohtsuka, Eiko
Several mutants for the minus strands of the selfcleaving domain of the satellite RNA of tobacco ringspot virus have been synthesized by joining chemically synthesized oligoribonucleotides with RNA...
Hakoshima, Toshio, Tanaka, Masahiro, Itoh, Takeshi, Tomita, Ken-khi, Amisaki, Takashi, Nishikawa, Satoshi, ...
Hydrophobic effects on binding of ribonuclease Tl to guanine bases of several ribonucleotides have been proved by mutating a hydrophobic residue at the recognition site and by measuring the effect on...
Itoh, Takeshi, Tomita, Ken-ichi, Hakoshima, Toshio, Hiroaki, Hidekazu, Uesugi, Sei-ichi, Nishikawa, Satoshi, ...
The crystal structure of a mutant ribonuclease T1(Y45W) complexed with a specific inhibitor, 2′GMP, has been determined by X-ray diffraction and refined at 1.9 A� resolution to a conventional...
Murata, Tetsuya, Iwai, Shigenori, Ohtsuka, Eiko
A dodecadeoxyribonucleotide containing a cis-syn thymine dimer with a phosphorodithioate linkage was synthesized on a solid support using a dinucleotide coupling unit prepared by UV-irradiation of...
Mayeda, Akila, Hayase, Yoji, Inoue, Hideo, Ohtsuka, Eiko, Ohshima, Yasumi
We chemically synthesized antisense 12 mer 2′-O-methylribonucleotides and surveyed a scanning (signal-tracking) process as well as sequences within a β-globin transcript acting in the splicing...
Hakoshima, Toshio, Oka, Ken-ichi, Toda, Shyoji, Tanaka, Masahiro, Goda, Keigo, Higo, Takashi, ...
Ribonuclease T1 and the mutant enzymes were cocrystallized with several ribonucleotides, including non-hydrolyzable substrate analogs of di- and triribonucleotides, which have a novel guanylate in...
Design of RNA enzymes distinguishing a single base mutation in RNA (1989)
Koizumi, Makoto, Hayase, Yoji, Iwai, Shigenori, Kamiya, Hiroyuki, Inoue, Hideo, Ohtsuka, Eiko
RNA enzymes (ribozymes) which can cleave RNA by recognizing sequences of 9–15 bases are described. Substrates must contain UX(X=U, C or A). A ribozyme consisting of two oligoribonucleotides (19 mer...
Hakoshima, Toshio, Toda, Shoji, Sugio, Shigetoshi, Tomita, Ken-ichi, Nishikawa, Satoshi, Morioka, Hiroshi, ...
Recognition by ribonuclease T1 of guanine bases via multidentate hydrogen bonding and stacking interactions appears to be mediated mainly by a short peptide segment formed by one stretch of a...
Miura, Kazunobu, Kamiya, Hiroyuki, Kubota, Sachie, Ikehara, Morio, Nishimura, Susumu, Ohtsuka, Eiko
A gene coding for the novel ras protein, p21x, in which the domains of guanine binding and phosphate binding were exchanged, was constructed and expressed in Escherichia coli. The gene product, p21x,...
Iwai, Shigenori, Ohtsuka, Eiko
The levulinyl group has been employed for protection of the 5'-hydroxyl group in the synthesis of oligoribonucleotides by the phosphoramidite approach, using the acid-labile 2'-tetrahydro-furanyl...
Jwai, Shigenori, Yamada, Emi, Asaka, Miyuki, Hayase, Yoji, Inoue, Hideo, Ohtsuka, Eiko
Six nonaribonucleotides containing the 5'-splice site, one complementary nonamer and an octadecamer containing the 3'-splice site have been synthesized on a polymer support using the...
Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides (1987)
Inoue, Hideo, Hayase, Yoji, Imura, Akihiro, Iwai, Shigenori, Miura, Kazunobu, Ohtsuka, Eiko
2′-O-Methyl derivatives of the common ribonucleosides except for guanosine were synthesized via the 2′-O-methylation of appropriately-protected nucleosides with CH3I in the presence of Ag2O. The...
Nishikawa, Satoshi, Yanase, Kohichi, Tokunaga-Doi, Tomoko, Kodama, Kazumi, Gomi, Hideyuki, Uesugi, Seiichi, ...
The gene for insulin-like growth factor I (IGF-I) was constructed from chemically synthesized deoxyoligonucleotides and expressed in Escherichia coli, under the control of a trp promoter, as a set of...
Site-directed cleavage of RNA (1987)
Shibahara, Susumu, Mukai, Sachiko, Nishihara, Tohru, Inoue, Hideo, Ohtsuka, Eiko, Morisawa, Hirokazu
Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2′-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with...
Structure- and sequence-specificity of ozone degradation of supercoiled plasmid DNA1 (1986)
Sawadaishi, Kazuyuki, Miura, Kazunobu, Ohtsuka, Eiko, Ueda, Tohm, Shinriki, Nariko, Ishizaki, Kozo
Ozone-reactive sites on the nucleobase moieties in supercoiled pBR322 DNA were investigated by using sequencing procedures. Ozonolysis in the absence of salt resulted in degradation of thymine...
Ozonolysis of supercoiled pBR322 DNA resulting in strand scission to open circular DNA1 (1985)
Sawadaishi, Kazuyuki, Miura, Kazunobu, Ohtsuka, Eiko, Ueda, Tohru, Ishizaki, Kazo, Shinriki, Nariko
Treatment of supercoiled pBR322 DNA with ozone resulted in the conversion of closed circular DNA to open circular DNA. Restriction analysis of the resulting open circular DNA showed that ozonolysis...
Doi, Takefumi, Yamane, Akio, Matsugi, Jitsuhiro, Ohtsuka, Eiko, Ikehara, Morio
Insertion of the four major nucleotides at the 5′-side of the anticodon triplet of E. coli tRNAMet was performed by joining of the half molecules obtained b limited digestion with RNase A and the...
LEE, Sang Jong, AKUTSU, Hideo, KYOGOKU, Yoshimasa, KITANO, Katsuhiko, TOZUKA, Zenzaburo, OHTA, Atsunori, ...
The imino proton resonances of λ OR3 17mer in water were observed at 500 Mhz with the time-shared Redfield pulse train. All of the 17 imino proton resonances could be assigned specifically to...
KUWANO, Ryozo, ARAKI, Kazuaki, USUI, Hiroshi, FUKUI, Toshikazu, OHTSUKA, Eiko, IKEHARA, Morio, ...
A mixture of 14-mer oligodeoxynucleotides was used for the screening of a cDNA clone coding for a cholecystokinin (CCK) precursor from a cDNA library for rat brain microsomal poly(A)RNA. The longest...
Uesugi, Seiichi, Ohkubo, Mitsuru, Ohtsuka, Eiko, Ikehara, Morio, Kobayashi, Yuji, Kyogoku, Yoshimasa
The poly [r(C-G)]duplex shows an unusually large negative CD band in the long wavelength region. in order to elucidate this phenomenon, r(C-G-C-G) and r(C-G-C-G-C-G) were synthesized by a...
Kuwano, Ryozo, Usui, Hiroshi, Maeda, Toshinaga, Fukui, Toshikazu, Yamanari, Nobuko, Ohtsuka, Eiko, ...
The complete nucleotide sequence of mRNA for β-subunit of rat brain S-100 protein was determined from recombinant cDNA clones. The sequence was composed of 1488 bp which Included the 276 bp of the...
Ohtsuka, Eiko, Yamane, Akio, Ikehara, Mono
2′-O-Tetrahydrofurany1-5′-O-dimethoxytrityl-N-protected nucleosides were phosphorylated to give the 3′-(o-chlorophenyl) phosphates which were then condensed with 3′,5′-unprotected...
Nishimura, Yoshifumi, Tsuboi, Masamichi, Nakano, Toru, Higuchi, Shigesada, Sato, Tomohiro, Shida, Toshio, ...
Observations of Raman spectra of various nucleic acids indicate that the guanine ring breathing frequency is sensitive to the internal rotation angle around the glycosidic bond and to the...
Aoyama, Takashi, Takanami, Mituru, Ohtsuka, Eiko, Taniyama, Yoshio, Marumoto, Ryuji, Sato, Hiroko, ...
A promoter with the consensus sequences(TTGACA and TATAAT) at −35 and −10 regions was constructed, and the distance between the two consensus sequences was independently altered at two...
Ohtsuka, Eiko, Doi, Takefumi, Fukumoto, Ryoichi, Matsugi, Jitsuhiro, Ikehara, Morio
E. coli tRNAfMet was hydrolyzed with RNase A using a limited amount of the enzyme to give two half molecules lacking the anticodon trimer and 3′-terminal dimer. Chemically synthesized trimers CUAp...
Ohtsuka, Eiko, Taniyama, Yoshio, Marumoto, Ryuji, Sato, Hiroko, Hirosaki, Haruhisa, Ikehara, Morio
A phosphorylating reagent o-chlorophenyl phosphoro-p-anisi-dochloridate was synthesized to phosphorylate the 3'-hydroxyl group of N, 5'-protected deoxynucleosides. These nucleotides served as...
E. coli initiator tRNA analogs with different nucleotides in the discriminator base position (1982)
Uemura, Haruki, Imai, Mayumi, Ohtsuka, Eiko, Ikehara, Morio, Söll, Dieter
The effect of base changes at the fourth position from the 3′-terminus of Esoheriohia coli initiator tRNA Met has been studied to test the ′discriminator hypothesis′ which proposed that the...
FUKUI, Toshikazu, ISHIHAMA, Akira, OHTSUKA, Eiko, IKEHARA, Morio, FUKUDA, Ryuji
The influence of oligodeoxynucleotide chain length on the priming activity of the reverse transcription was investigated in poly(A)-dependent poly(dT), poly(C)-dependent poly(dG) and influenza virus...
Characterization of nascent DNA fragments produced by excision of uracil residues in DNA (1981)
Machida, Yasunori, Okazaki, Tuneko, Miyake, Tetsuo, Ohtsuka, Eiko, Ikehara, Morio
Nascent short DNA chains could result from repair of incorporated uracil residues or be intermediates in discontinuous replication. We have characterized short DNA chains having...
KAWAKAMI, Kiyoshi, ISHIHAMA, Akira, OHTSUKA, Eiko, TANAKA, Toshiki, TAKASHIMA, Hideaki, IKEHARA, Morio
The virion-associated RNA-dependent RNA polymerase of influenza virus PR8 was activated by treatment with specific non-ionic detergents, and was remarkably stimulated by exogenously added...
Ohtsuka, Eiko, Doi, Takefumi, Uemura, Haruki, Taniyama, Yoshio, Ikehara, Morio
A tetradecanucleotide U-A-G-C (U-C-G)2G-G-C-Up corresponding to bases 21–34 of a nascent sequence of formylmethionyl tRNA of E. coli has been synthesized by the joining of two combinations of...
ISHIHAMA, Akira, ENAMI, Masayoshi, NISHIJIMA, Yoshikazu, FUKUI, Toshikazu, OHTSUKA, Eiko, IKEHARA, Morio
The effects of 2′-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the...
Synthesis of E.coli tRNAfMet fragments and their joining with RNA ligase (1978)
Ohtsuka, Eiko, Tanaka, Toshiki, Miyake, Tetsuo, Nakagawa, Eiko, Antkowiak, Janina, Wakabayashi, Toshiaki, ...
Oligonucleotides corresponding to the E. coli tRNAfMet have been synthesized. A diester approach has been used for the synthesis of 6, 7, 8 and 9. A block condensation of nucleotides with...
Ikehara, Morio, Tanaka, Shoji, Fukui, Toshikazu, Ohtsuka, Eiko
2′-O-o-Nitrobenzyluridine, -cytidine and -adenosine were phosphorylated with phosphoryl chloride to the corresponding 5′-phosphates and led to 5′-diphosphates by the method of Moffatt and...
Ohtsuka, Eiko, Nishikawa, Satoshi, Sugiura, Masahiro, Ikehara, Morio
T4 RNA ligase was found to join A-A-A-A-A-A and 32pU-U-U-U in the presence of ATP as cofactor. In this reaction the pyrophosphate of *pU-U-U-U and pA was isolated by chromatography on a RPC-5 column,...
Ohtsuka, Eiko, Nakamura, Sojiro, Yoneda, Mariko, Ikehara, Morio
Guanylyl-(3′-5′)-cytidine and guanylyl-(2′-5′)-cytidine were synthesized by condensing 5′-O-monamethoxytritylguanosine and N,2′,3′-O-triacetylcytidine 5′-phosphate using...
ONISHI, Hirofumi, OHTSUKA, Eiko, IKEHARA, Morio, TONOMURA, Yuji
1. The hydrolysis of 1, N6-ethenoadenosine triphosphate (ϵATP) by myosin [EC 3.6.1.3] and the change in fluorescence of ϵATP induced by H-meromyosin were studied. (a) The amount of...
Torizawa, Takuya, Yamamoto, Nobuhiro, Suzuki, Tomonori, Nobuoka, Kaoru, Komatsu, Yasuo, Morioka, Hiroshi, ...
Monoclonal antibodies specific for the cyclobutane pyrimidine dimer (CPD) are widely used for detection and quantification of DNA photolesions. However, the mechanisms of antigen binding by anti-CPD...
Ling, Feng, Morioka, Hiroshi, Ohtsuka, Eiko, Shibata, Takehiko
A nuclear recessive mutant in Saccharomyces cerevisiae, mhr1-1, is defective in mitochondrial genetic recombination at 30°C and shows extensive vegetative petite induction by UV irradiation at 30°C...
Sakamoto, Satoshi, Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Kitamura, Masaya, ...
Based on our recent studies of RNA cleavage by oligonucleotide–terpyridine·Cu(II) complex 5′- and/or 3′-conjugates, we designed 2′-O-methyloligonucleotides with two terpyridine-attached...
Kojima, Naoshi, Inoue, Kaori, Nakajima-Shibata, Rina, Kawahara, Shun-ichi, Ohtsuka, Eiko
The first synthesis of 5-amino-3-(2′-deoxy-β-d-ribofuranosyl)imidazo[4,5-b]pyridin-7-one (1-deaza-2′-deoxyguanosine) is described. The compound was converted from the known AICA-deoxyriboside....
Structural basis for recruitment of human flap endonuclease 1 to PCNA
Sakurai, Shigeru, Kitano, Ken, Yamaguchi, Hiroto, Hamada, Keisuke, Okada, Kengo, Fukuda, Kotaro, ...
Flap endonuclease-1 (FEN1) is a key enzyme for maintaining genomic stability and replication. Proliferating cell nuclear antigen (PCNA) binds FEN1 and stimulates its endonuclease activity. The...
Torizawa, Takuya, Yamamoto, Nobuhiro, Suzuki, Tomonori, Nobuoka, Kaoru, Komatsu, Yasuo, Morioka, Hiroshi, ...
Monoclonal antibodies specific for the cyclobutane pyrimidine dimer (CPD) are widely used for detection and quantification of DNA photolesions. However, the mechanisms of antigen binding by anti-CPD...
Ling, Feng, Morioka, Hiroshi, Ohtsuka, Eiko, Shibata, Takehiko
A nuclear recessive mutant in Saccharomyces cerevisiae, mhr1-1, is defective in mitochondrial genetic recombination at 30°C and shows extensive vegetative petite induction by UV irradiation at 30°C...
Sakamoto, Satoshi, Tamura, Takashi, Furukawa, Takako, Komatsu, Yasuo, Ohtsuka, Eiko, Kitamura, Masaya, ...
Based on our recent studies of RNA cleavage by oligonucleotide–terpyridine·Cu(II) complex 5′- and/or 3′-conjugates, we designed 2′-O-methyloligonucleotides with two terpyridine-attached...
Kojima, Naoshi, Inoue, Kaori, Nakajima-Shibata, Rina, Kawahara, Shun-ichi, Ohtsuka, Eiko
The first synthesis of 5-amino-3-(2′-deoxy-β-d-ribofuranosyl)imidazo[4,5-b]pyridin-7-one (1-deaza-2′-deoxyguanosine) is described. The compound was converted from the known AICA-deoxyriboside....
Structural basis for recruitment of human flap endonuclease 1 to PCNA
Sakurai, Shigeru, Kitano, Ken, Yamaguchi, Hiroto, Hamada, Keisuke, Okada, Kengo, Fukuda, Kotaro, ...
Flap endonuclease-1 (FEN1) is a key enzyme for maintaining genomic stability and replication. Proliferating cell nuclear antigen (PCNA) binds FEN1 and stimulates its endonuclease activity. The...