Eleftherios Mylonakis, Arturo Casadevall, Frederick M. Ausubel
Experiments with insects, protozoa, nematodes, and slime molds have recently come to the forefront in the study of host–fungal interactions. Many of the virulence factors required for pathogenicity...
Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay (2007)
Julia Breger, Beth Burgwyn Fuchs, George Aperis, Terence I. Moy, Frederick M. Ausubel, Eleftherios Mylonakis
There is an urgent need for the development of new antifungal agents. A facile in vivo model that evaluates libraries of chemical compounds could solve some of the main obstacles in current...
Mylonakis, Eleftherios, Rude, Thomas, Toffaletti, Dena, Cox, Gary M, ...
The disaccharide trehalose has been found to play diverse roles, from energy source to stress protectant, and this sugar is found in organisms as diverse as bacteria, fungi, plants, and invertebrates...
Chodock, Rochelle, Mylonakis, Eleftherios, Shemin, Douglas, Runarsdottir, Valgerdur, Yodice, Paul, Renzi, Richard, ...
A simple model host for identifying Gram-positive virulence factors
Garsin, Danielle A., Sifri, Costi D., Mylonakis, Eleftherios, Qin, Xiang, Singh, Kavindra V., Murray, Barbara E., ...
We demonstrate the use of the nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus...
Mylonakis, Eleftherios, Engelbert, Michael, Qin, Xiang, Sifri, Costi D., Murray, Barbara E., Ausubel, Frederick M., ...
We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion...
Sifri, Costi D., Mylonakis, Eleftherios, Singh, Kavindra V., Qin, Xiang, Garsin, Danielle A., Murray, Barbara E., ...
The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing...
Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis
Mylonakis, Eleftherios, Ausubel, Frederick M., Perfect, John R., Heitman, Joseph, Calderwood, Stephen B.
We found that the well-studied nematode Caenorhabditis elegans can use various yeasts, including Cryptococcus laurentii and Cryptococcus kuetzingii, as a sole source of food, producing similar brood...
Apidianakis, Yiorgos, Rahme, Laurence G., Heitman, Joseph, Ausubel, Frederick M., Calderwood, Stephen B., Mylonakis, Eleftherios
We found that the ingestion of Cryptococcus neoformans by Drosophila melanogaster resulted in the death of the fly but that the ingestion of Saccharomyces cerevisiae or the nonpathogenic Cryptococcus...
Engelbert, Michael, Mylonakis, Eleftherios, Ausubel, Frederick M., Calderwood, Stephen B., Gilmore, Michael S.
Gelatinase and serine protease were found to contribute in concert to pathogenesis in a rabbit model of endophthalmitis. However, a mutant defective in the fsr regulator was observed to be more...
Bhabhra, Ruchi, Miley, Michael D., Mylonakis, Eleftherios, Boettner, Doug, Fortwendel, Jarrod, Panepinto, John C., ...
Aspergillus fumigatus CgrA is the ortholog of a yeast nucleolar protein that functions in ribosome synthesis. To determine how CgrA contributes to the virulence of A. fumigatus, a ΔcgrA mutant was...
Cytotoxicity of Hydrogen Peroxide Produced by Enterococcus faecium
Moy, Terence I., Mylonakis, Eleftherios, Calderwood, Stephen B., Ausubel, Frederick M.
Although the opportunistic bacterial pathogen Enterococcus faecium is a leading source of nosocomial infections, it appears to lack many of the overt virulence factors produced by other bacterial...
Nielsen, Kirsten, Cox, Gary M., Litvintseva, Anastasia P., Mylonakis, Eleftherios, Malliaris, Stephanie D., Benjamin, Daniel K., ...
Cryptococcus neoformans is a fungal pathogen that has evolved over the past 40 million years into three distinct varieties or sibling species (gattii, grubii, and neoformans). Each variety manifests...
Tang, Robin J., Breger, Julia, Idnurm, Alexander, Gerik, Kimberly J., Lodge, Jennifer K., Heitman, Joseph, ...
Caenorhabditis elegans can serve as a substitute host for the study of microbial pathogenesis. We found that mutations in genes of the fungal pathogen Cryptococcus neoformans involved in mammalian...
Galleria mellonella as a Model System To Study Cryptococcus neoformans Pathogenesis
Mylonakis, Eleftherios, Moreno, Roberto, El Khoury, Joseph B., Idnurm, Alexander, Heitman, Joseph, Calderwood, Stephen B., ...
Evaluation of Cryptococcus neoformans virulence in a number of nonmammalian hosts suggests that C. neoformans is a nonspecific pathogen. We used the killing of Galleria mellonella (the greater wax...
A simple model host for identifying Gram-positive virulence factors
Garsin, Danielle A., Sifri, Costi D., Mylonakis, Eleftherios, Qin, Xiang, Singh, Kavindra V., Murray, Barbara E., ...
We demonstrate the use of the nematode Caenorhabditis elegans as a facile and inexpensive model host for several Gram-positive human bacterial pathogens. Enterococcus faecalis, Streptococcus...
Mylonakis, Eleftherios, Engelbert, Michael, Qin, Xiang, Sifri, Costi D., Murray, Barbara E., Ausubel, Frederick M., ...
We used a rabbit endophthalmitis model to explore the role of fsrB, a gene required for the function of the fsr quorum-sensing system of Enterococcus faecalis, in pathogenicity. A nonpolar deletion...
Sifri, Costi D., Mylonakis, Eleftherios, Singh, Kavindra V., Qin, Xiang, Garsin, Danielle A., Murray, Barbara E., ...
The expression of two Enterococcus faecalis extracellular virulence-related proteins, gelatinase (GelE) and serine protease (SprE), has been shown to be positively regulated by the fsr quorum-sensing...
Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis
Mylonakis, Eleftherios, Ausubel, Frederick M., Perfect, John R., Heitman, Joseph, Calderwood, Stephen B.
We found that the well-studied nematode Caenorhabditis elegans can use various yeasts, including Cryptococcus laurentii and Cryptococcus kuetzingii, as a sole source of food, producing similar brood...
Apidianakis, Yiorgos, Rahme, Laurence G., Heitman, Joseph, Ausubel, Frederick M., Calderwood, Stephen B., Mylonakis, Eleftherios
We found that the ingestion of Cryptococcus neoformans by Drosophila melanogaster resulted in the death of the fly but that the ingestion of Saccharomyces cerevisiae or the nonpathogenic Cryptococcus...
Engelbert, Michael, Mylonakis, Eleftherios, Ausubel, Frederick M., Calderwood, Stephen B., Gilmore, Michael S.
Gelatinase and serine protease were found to contribute in concert to pathogenesis in a rabbit model of endophthalmitis. However, a mutant defective in the fsr regulator was observed to be more...
Bhabhra, Ruchi, Miley, Michael D., Mylonakis, Eleftherios, Boettner, Doug, Fortwendel, Jarrod, Panepinto, John C., ...
Aspergillus fumigatus CgrA is the ortholog of a yeast nucleolar protein that functions in ribosome synthesis. To determine how CgrA contributes to the virulence of A. fumigatus, a ΔcgrA mutant was...
Cytotoxicity of Hydrogen Peroxide Produced by Enterococcus faecium
Moy, Terence I., Mylonakis, Eleftherios, Calderwood, Stephen B., Ausubel, Frederick M.
Although the opportunistic bacterial pathogen Enterococcus faecium is a leading source of nosocomial infections, it appears to lack many of the overt virulence factors produced by other bacterial...
Galleria mellonella as a Model System To Study Cryptococcus neoformans Pathogenesis
Mylonakis, Eleftherios, Moreno, Roberto, El Khoury, Joseph B., Idnurm, Alexander, Heitman, Joseph, Calderwood, Stephen B., ...
Evaluation of Cryptococcus neoformans virulence in a number of nonmammalian hosts suggests that C. neoformans is a nonspecific pathogen. We used the killing of Galleria mellonella (the greater wax...
Nielsen, Kirsten, Cox, Gary M., Litvintseva, Anastasia P., Mylonakis, Eleftherios, Malliaris, Stephanie D., Benjamin, Daniel K., ...
Cryptococcus neoformans is a fungal pathogen that has evolved over the past 40 million years into three distinct varieties or sibling species (gattii, grubii, and neoformans). Each variety manifests...
Tang, Robin J., Breger, Julia, Idnurm, Alexander, Gerik, Kimberly J., Lodge, Jennifer K., Heitman, Joseph, ...
Caenorhabditis elegans can serve as a substitute host for the study of microbial pathogenesis. We found that mutations in genes of the fungal pathogen Cryptococcus neoformans involved in mammalian...
Petzold, Elizabeth Wills, Himmelreich, Uwe, Mylonakis, Eleftherios, Rude, Thomas, Toffaletti, Dena, Cox, Gary M., ...
The disaccharide trehalose has been found to play diverse roles, from energy source to stress protectant, and this sugar is found in organisms as diverse as bacteria, fungi, plants, and invertebrates...
Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay
Breger, Julia, Fuchs, Beth Burgwyn, Aperis, George, Moy, Terence I, Ausubel, Frederick M, Mylonakis, Eleftherios
There is an urgent need for the development of new antifungal agents. A facile in vivo model that evaluates libraries of chemical compounds could solve some of the main obstacles in current...
The Temperature-Sensitive Role of Cryptococcus neoformans ROM2 in Cell Morphogenesis
Fuchs, Beth Burgwyn, Tang, Robin J., Mylonakis, Eleftherios
ROM2 is associated with Cryptococcus neoformans virulence. We examined additional roles of ROM2 in C. neoformans and found that ROM2 plays a role in several cell functions specifically at high...
Mylonakis, Eleftherios, Casadevall, Arturo, Ausubel, Frederick M
Experiments with insects, protozoa, nematodes, and slime molds have recently come to the forefront in the study of host–fungal interactions. Many of the virulence factors required for pathogenicity...
Fan, Weihua, Idnurm, Alexander, Breger, Julia, Mylonakis, Eleftherios, Heitman, Joseph
The basidiomycetous fungal pathogen Cryptococcus neoformans is adapted to survive challenges in the soil and environment and within the unique setting of the mammalian host. A C. neoformans mutant...
Fuchs, Beth Burgwyn, Tegos, George P., Hamblin, Michael R., Mylonakis, Eleftherios
Photodynamic therapy is a rapidly developing antimicrobial technology which combines a nontoxic photoactivatable dye or photosensitizer with harmless visible light of the correct wavelength to excite...
Prokaryote–eukaryote interactions identified by using Caenorhabditis elegans
Peleg, Anton Y., Tampakakis, Emmanouil, Fuchs, Beth Burgwyn, Eliopoulos, George M., Moellering, Robert C., Mylonakis, Eleftherios
Prokaryote–eukaryote interactions are ubiquitous and have important medical and environmental significance. Despite this, a paucity of data exists on the mechanisms and pathogenic consequences of...
Efflux in Fungi: La Pièce de Résistance
Coleman, Jeffrey J., Mylonakis, Eleftherios
Pathogens must be able to overcome both host defenses and antimicrobial treatment in order to successfully infect and maintain colonization of the host. One way fungi accomplish this feat and...
Ziakas, Panayiotis D., Karsaliakos, Petros, Mylonakis, Eleftherios
Previous observations indicate that a lamividune-prophylaxis strategy results in a decrease of hepatitis B virus (HBV) reactivation rates. This report evaluates the benefits from this strategy among...
Cowen, Leah E., Singh, Sheena D., Köhler, Julia R., Collins, Cathy, Zaas, Aimee K., Schell, Wiley A., ...
Invasive fungal infections are a leading cause of mortality among immunocompromised individuals. Treatment is notoriously difficult with the limited armamentarium of antifungal drugs, whose efficacy...
Okoli, Ikechukwu, Coleman, Jeffrey J., Tempakakis, Emmanouil, An, W. Frank, Holson, Edward, Wagner, Florence, ...
Candida albicans, the most common human pathogenic fungus, can establish a persistent lethal infection in the intestine of the microscopic nematode Caenorhabditis elegans. The C. elegans–C....
Means, Terry K., Mylonakis, Eleftherios, Tampakakis, Emmanouil, Colvin, Richard A., Seung, Edward, Puckett, Lindsay, ...
Receptors involved in innate immunity to fungal pathogens have not been fully elucidated. We show that the Caenorhabditis elegans receptors CED-1 and C03F11.3, and their mammalian orthologues, the...
Tampakakis, Emmanouil, Peleg, Anton Y., Mylonakis, Eleftherios
Candida albicans is an opportunistic human fungal pathogen that normally resides in the gastrointestinal tract and on the skin as a commensal but can cause life-threatening invasive disease....