Elena Conti

Disentangling Reticulate Evolution in an Arctic-Alpine Polyploid Complex (2009)

Guggisberg, Alessia, Mansion, Guilhem, Conti, Elena

Although polyploidy plays a fundamental role in plant evolution, the elucidation of polyploid origins is fraught with methodological challenges. For example, allopolyploid species may confound...

The C-terminal region of Ge-1 presents conserved structural features required for P-body localization (2008)

Jinek, Martin, Eulalio, Ana, Lingel, Andreas, Helms, Sigrun, Conti, Elena, Izaurralde, Elisa

The removal of the 5′ cap structure by the DCP1–DCP2 decapping complex irreversibly commits eukaryotic mRNAs to degradation. In human cells, the interaction between DCP1 and DCP2 is bridged by...

Phylogenetic Analysis Informed by Geological History Supports Multiple, Sequential Invasions of the Mediterranean Basin by the Angiosperm Family Araceae (2008)

Mansion, Guilhem, Rosenbaum, Gideon, Schoenenberger, Nicola, Bacchetta, Gianluigi, Rosselló, Josep A., Conti, Elena

Despite the remarkable species richness of the Mediterranean flora and its well-known geological history, few studies have investigated its temporal and spatial origins. Most importantly, the...

Genomic Origin and Organization of the Allopolyploid Primula egaliksensis Investigated by in situ Hybridization (2008)

Guggisberg, Alessia, Baroux, Célia, Grossniklaus, Ueli, Conti, Elena

Background and Aims Earlier studies have suggested that the tetraploid Primula egaliksensis (2n = 40) originated from hybridization between the diploids P. mistassinica (2n = 18) and P. nutans (2n =...

PHYLOGENY AND SYSTEMATICS OF THE RAUVOLFIOIDEAE (APOCYNACEAE) BASED ON MOLECULAR AND MORPHOLOGICAL EVIDENCE1 (2007)

André O. Simões, Tatyana Livshultz, Elena Conti, Mary E. Endress

To elucidate deeper relationships within Rauvolfioideae (Apocynaceae), a phylogenetic analysis was conducted using sequences from five DNA regions of the chloroplast genome (matK, rbcL, rpl16 intron,...

Assessing Calibration Uncertainty in Molecular Dating: The Assignment of Fossils to Alternative Calibration Points (2007)

Rutschmann, Frank, Eriksson, Torsten, Salim, Kamariah Abu, Conti, Elena

Although recent methodological advances have allowed the incorporation of rate variation in molecular dating analyses, the calibration procedure, performed mainly through fossils, remains resistant...

IS MANDEVILLA (APOCYNACEAE, MESECHITEAE) MONOPHYLETIC? EVIDENCE FROM FIVE PLASTID DNA LOCI AND MORPHOLOGY1 (2006)

André O. Simões, Mary E. Endress, Luiza S. Kinoshita, Elena Conti

In order to test the monophyly of Mandevilla Lindl., the largest genus in tribe Mesechiteae (Apocynaceae, Apocynoideae), and its affinities to other genera in the tribe, maximum parsimony...

EARLY TERTIARY OUT-OF-INDIA DISPERSAL OF CRYPTERONIACEAE: EVIDENCE FROM PHYLOGENY AND MOLECULAR DATING (2002)

Elena Conti, Torsten Eriksson, Jürg Schönenberger, Kenneth J. Sytsma, David A. Baum

Phylogenetic analyses and molecular dating estimates based on chloroplast DNA sequences were used to establish the relationships of the southern and Southeast Asian Crypteroniaceae and elucidate...

The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain

Liker, Erika, Fernandez, Elena, Izaurralde, Elisa, Conti, Elena

Human TAP is implicated in mRNA nuclear export and is used by simian type D retroviruses to export their unspliced genomic RNA to the cytoplasm of the host cell. We have determined the crystal...

Molecular insights into the interaction of PYM with the Mago–Y14 core of the exon junction complex

Bono, Fulvia, Ebert, Judith, Unterholzner, Leonie, Güttler, Thomas, Izaurralde, Elisa, Conti, Elena

The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago–Y14 heterodimer is a core component of the EJC. Recently,...

Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15

Fribourg, Sébastien, Conti, Elena

The association between Mtr2 and Mex67 is essential for the nuclear export of bulk messenger RNA in yeast. In metazoans, the analogous function is carried out by the TAP–p15 heterodimer. Whereas...

The structure of the mRNA export factor TAP reveals a cis arrangement of a non-canonical RNP domain and an LRR domain

Liker, Erika, Fernandez, Elena, Izaurralde, Elisa, Conti, Elena

Human TAP is implicated in mRNA nuclear export and is used by simian type D retroviruses to export their unspliced genomic RNA to the cytoplasm of the host cell. We have determined the crystal...

Molecular insights into the interaction of PYM with the Mago–Y14 core of the exon junction complex

Bono, Fulvia, Ebert, Judith, Unterholzner, Leonie, Güttler, Thomas, Izaurralde, Elisa, Conti, Elena

The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago–Y14 heterodimer is a core component of the EJC. Recently,...

Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15

Fribourg, Sébastien, Conti, Elena

The association between Mtr2 and Mex67 is essential for the nuclear export of bulk messenger RNA in yeast. In metazoans, the analogous function is carried out by the TAP–p15 heterodimer. Whereas...

Structures of the PIN domains of SMG6 and SMG5 reveal a nuclease within the mRNA surveillance complex

Glavan, Filip, Behm-Ansmant, Isabelle, Izaurralde, Elisa, Conti, Elena

SMG6 and SMG5 are essential factors in nonsense-mediated mRNA decay, a conserved pathway that degrades mRNAs with premature translation termination codons. Both SMG5 and SMG6 have been predicted to...

Structural and biochemical characterization of the yeast exosome component Rrp40

Oddone, Anna, Lorentzen, Esben, Basquin, Jerome, Gasch, Alexander, Rybin, Vladimir, Conti, Elena, ...

The exosome is a protein complex that is important in both degradation and 3′-processing of eukaryotic RNAs. We present the crystal structure of the Rrp40 exosome subunit from Saccharomyces...

RNA channelling by the archaeal exosome

Lorentzen, Esben, Dziembowski, Andrzej, Lindner, Doris, Seraphin, Bertrand, Conti, Elena

Exosomes are complexes containing 3′ → 5′ exoribonucleases that have important roles in processing, decay and quality control of various RNA molecules. Archaeal exosomes consist of a hexameric...

Eukaryotic expression, purification, crystallization and preliminary X-ray analysis of murine Manic Fringe

Jinek, Martin, Conti, Elena

The catalytic domain of the murine glycosyltransferase Manic Fringe was expressed in insect cells. Removal by site-directed mutagenesis of two N-glycosylation sites present in the protein was...

The C-terminal region of Ge-1 presents conserved structural features required for P-body localization

Jinek, Martin, Eulalio, Ana, Lingel, Andreas, Helms, Sigrun, Conti, Elena, Izaurralde, Elisa

The removal of the 5′ cap structure by the DCP1–DCP2 decapping complex irreversibly commits eukaryotic mRNAs to degradation. In human cells, the interaction between DCP1 and DCP2 is bridged by...

Stable kinetochore–microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3

Gaitanos, Thomas N, Santamaria, Anna, Jeyaprakash, A Arockia, Wang, Bin, Conti, Elena, Nigg, Erich A

Ska1 and Ska2 form a complex at the kinetochore–microtubule (KT–MT) interface and are required for timely progression from metaphase to anaphase. Here, we use mass spectrometry to search for...