Elisabeth Jamet

Cell wall biogenesis of Arabidopsis thalianaelongating cells: transcriptomics complements proteomics (2009)

Jamet, Elisabeth, Roujol, David, San-Clemente, Hélène, Irshad, Muhammad, Soubigou-Taconnat, Ludivine, Renou, Jean-Pierre, ...

Abstract Background Plant growth is a complex process involving cell division and elongation. Arabidopsis thaliana hypocotyls undergo a 100-fold length increase mainly by cell elongation. Cell...

Transcriptomic analysis of Arabidopsis developing stems: a close-up on cell wall genes (2009)

Minic, Zoran, Jamet, Elisabeth, San-Clemente, Hélène, Pelletier, Sandra, Renou, Jean-Pierre, Rihouey, Christophe, ...

Abstract Background Different strategies (genetics, biochemistry, and proteomics) can be used to study proteins involved in cell biogenesis. The availability of the complete sequences of several...

Bioinformatics as a Tool for Assessing the Quality of Sub-Cellular Proteomic Strategies and Inferring Functions of Proteins: Plant Cell Wall Proteomics as a Test Case (2009)

San Clemente, Hélène, Pont-Lezica, Rafael F., Jamet, Elisabeth

Bioinformatics is used at three different steps of proteomic studies of sub-cellular compartments. First one is protein identifi cation from mass spectrometry data. Second one is prediction of...

Bioinformatics as a Tool for Assessing the Quality of Sub-Cellular Proteomic Strategies and Inferring Functions of Proteins: Plant Cell Wall Proteomics as a Test Case (2009)

San Clemente, Hélène, Pont-Lezica, Rafael F., Jamet, Elisabeth

Bioinformatics is used at three different steps of proteomic studies of sub-cellular compartments. First one is protein identifi cation from mass spectrometry data. Second one is prediction of...

Bioinformatics as a Tool for Assessing the Quality of Sub-Cellular Proteomic Strategies and Inferring Functions of Proteins: Plant Cell Wall Proteomics as a Test Case (2009)

Hélène San Clemente, Rafael Pont-Lezica, Elisabeth Jamet

Bioinformatics is used at three different steps of proteomic studies of sub-cellular compartments. First one is protein identification from mass spectrometry data. Second one is prediction of...

Transcriptomic analysis of Arabidopsis developing stems: a close-up on cell wall genes. (2009)

Minic, Zoran, Jamet, Elisabeth, San-Clemente, Helene, Pelletier, Sandra, Renou, Jean-Pierre, Rihouey, Christophe, ...

BACKGROUND: Different strategies (genetics, biochemistry, and proteomics) can be used to study proteins involved in cell biogenesis. The availability of the complete sequences of several plant...

Transcriptomic analysis of Arabidopsis developing stems: a close-up on cell wall genes. (2009)

Minic, Zoran, Jamet, Elisabeth, San-Clemente, Helene, Pelletier, Sandra, Renou, Jean-Pierre, Rihouey, Christophe, ...

BACKGROUND: Different strategies (genetics, biochemistry, and proteomics) can be used to study proteins involved in cell biogenesis. The availability of the complete sequences of several plant...

Plant Cell Wall Proteomics: Mass Spectrometry Data, a Trove for Research on Protein Structure/Function Relationships (2009)

Albenne, Cécile, Canut, Hervé, Boudart, Georges, Zhang, Yu, San Clemente, Hélène, Pont-Lezica, Rafael, ...

Proteomics allows the large-scale study of protein expression either in whole organisms or in purified organelles. In particular, mass spectrometry (MS) analysis of gel-separated proteins produces...

Cell wall biogenesis of Arabidopsis thaliana elongating cells: transcriptomics complements proteomics. (2009)

Jamet, Elisabeth, Roujol, David, San Clemente, Hélène, Irshad, Muhammad, Soubigou-Taconnat, Ludivine, Renou, Jean-Pierre, ...

BACKGROUND: Plant growth is a complex process involving cell division and elongation. Arabidopsis thaliana hypocotyls undergo a 100-fold length increase mainly by cell elongation. Cell enlargement...

Cell wall biogenesis of Arabidopsis thaliana elongating cells: transcriptomics complements proteomics. (2009)

Jamet, Elisabeth, Roujol, David, San Clemente, Hélène, Irshad, Muhammad, Soubigou-Taconnat, Ludivine, Renou, Jean-Pierre, ...

BACKGROUND: Plant growth is a complex process involving cell division and elongation. Arabidopsis thaliana hypocotyls undergo a 100-fold length increase mainly by cell elongation. Cell enlargement...

A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: Confirmed actors and newcomers (2008)

Irshad, Muhammad, Canut, Hervé, Borderies, Gisèle, Pont-Lezica, Rafael, Jamet, Elisabeth

Abstract Background Cell elongation in plants requires addition and re-arrangements of cell wall components. Even if some protein families have been shown to play roles in these events, a global...

Recent advances in plant cell wall proteomics. (2008)

Jamet, Elisabeth, Albenne, Cécile, Boudart, Georges, Irshad, Muhammad, Canut, Hervé, Pont-Lezica, Rafael

The plant extracellular matrix contains typical polysaccharides such as cellulose, hemicelluloses, and pectins that interact to form dense interwoven networks. Plant cell walls play crucial roles...

Recent advances in plant cell wall proteomics. (2008)

Jamet, Elisabeth, Albenne, Cécile, Boudart, Georges, Irshad, Muhammad, Canut, Hervé, Pont-Lezica, Rafael

The plant extracellular matrix contains typical polysaccharides such as cellulose, hemicelluloses, and pectins that interact to form dense interwoven networks. Plant cell walls play crucial roles...

Cell Wall (2008)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Albenne, Cécile, Pont-Lezica, Rafael F.

This chapter covers our present knowledge of cell wall proteomics highlighting the distinctive features of cell walls and cell wall proteins in relation to problems encountered for protein...

Cell Wall (2008)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Albenne, Cécile, Pont-Lezica, Rafael F.

This chapter covers our present knowledge of cell wall proteomics highlighting the distinctive features of cell walls and cell wall proteins in relation to problems encountered for protein...

Partial purification and characterization of a copper-induced anionic peroxidase of sunflower roots (2008)

Jouili, Hager, Bouazizi, Houda, Rossignol, Michel, Borderies, Gisèle, Jamet, Elisabeth, El Ferjani, Ezzeddine

Treatment of 14 day-old sunflower seedlings with a toxic amount of copper (50 µM of CuSO4) during 5 days caused significant increase in peroxidase activity in roots. Qualitative analysis of soluble...

Partial purification and characterization of a copper-induced anionic peroxidase of sunflower roots (2008)

Jouili, Hager, Bouazizi, Houda, Rossignol, Michel, Borderies, Gisèle, Jamet, Elisabeth, El Ferjani, Ezzeddine

Treatment of 14 day-old sunflower seedlings with a toxic amount of copper (50 µM of CuSO4) during 5 days caused significant increase in peroxidase activity in roots. Qualitative analysis of soluble...

A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: confirmed actors and newcomers. (2008)

Irshad, Muhammad, Canut, Hervé, Borderies, Gisèle, Pont-Lezica, Rafael, Jamet, Elisabeth

BACKGROUND: Cell elongation in plants requires addition and re-arrangements of cell wall components. Even if some protein families have been shown to play roles in these events, a global picture of...

A new picture of cell wall protein dynamics in elongating cells of Arabidopsis thaliana: confirmed actors and newcomers. (2008)

Irshad, Muhammad, Canut, Hervé, Borderies, Gisèle, Pont-Lezica, Rafael, Jamet, Elisabeth

BACKGROUND: Cell elongation in plants requires addition and re-arrangements of cell wall components. Even if some protein families have been shown to play roles in these events, a global picture of...

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases (2007)

Minic, Zoran, Jamet, Elisabeth, Négroni, Luc, Zivy, Michel, Jouanin, Lise

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using...

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases. (2007)

Minic, Zoran, Jamet, Elisabeth, Négroni, Luc, Zivy, Michel, Jouanin, Lise

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using...

Cell Wall Proteome (2007)

Boudart, Georges, Minic, Zoran, Albenne, Cécile, Canut, Hervé, Jamet, Elisabeth, Pont-Lezica, Rafael F

In this chapter, we will focus on the contribution of proteomics to the identification and determination of the structure and function of CWPs as well as discussing new perspectives in this area. The...

Isolation of plant cell wall proteins (2007)

Jamet, Elisabeth, Boudart, Georges, Borderies, Gisèle, Charmont, Stéphane, Lafitte, Claude, Rossignol, Michel, ...

The quality of a proteomic analysis of a cell compartment strongly depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific...

Isolation of plant cell wall proteins (2007)

Jamet, Elisabeth, Boudart, Georges, Borderies, Gisèle, Charmont, Stéphane, Lafitte, Claude, Rossignol, Michel, ...

The quality of a proteomic analysis of a cell compartment strongly depends on the reliability of the isolation procedure for the cell compartment of interest. Plant cell walls possess specific...

Cell Wall Proteome (2007)

Boudart, Georges, Minic, Zoran, Albenne, Cécile, Canut, Hervé, Jamet, Elisabeth, Pont-Lezica, Rafael F

In this chapter, we will focus on the contribution of proteomics to the identification and determination of the structure and function of CWPs as well as discussing new perspectives in this area. The...

A sub-proteome of Arabidopsis thaliana mature stems trapped on Concanavalin A is enriched in cell wall glycoside hydrolases. (2007)

Minic, Zoran, Jamet, Elisabeth, Négroni, Luc, Zivy, Michel, Jouanin, Lise

N-glycosylated proteins were isolated from Arabidopsis thaliana mature stems using affinity chromatography on Concanavalin A Sepharose, separated by 2D-electrophoresis and identified using...

Cell wall proteins: a new insight through proteomics (2006)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Pont-Lezica, Rafael F

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

Abstract Background The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments....

Cell wall proteins: a new insight through proteomics. (2006)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Pont-Lezica, Rafael F

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins...

Cell wall proteins: a new insight through proteomics. (2006)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Pont-Lezica, Rafael F

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Cell wall proteins: a new insight through proteomics. (2006)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Pont-Lezica, Rafael F

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Evaluation of cell wall preparations for proteomics: a new procedure for purifying cell walls from Arabidopsis hypocotyls. (2006)

Feiz, Leila, Irshad, Muhammad, Pont-Lezica, Rafael F, Canut, Hervé, Jamet, Elisabeth

The ultimate goal of proteomic analysis of a cell compartment should be the exhaustive identification of resident proteins; excluding proteins from other cell compartments. Plant cell walls possess...

Cell wall proteins: a new insight through proteomics. (2006)

Jamet, Elisabeth, Canut, Hervé, Boudart, Georges, Pont-Lezica, Rafael F

Cell wall proteins are essential constituents of plant cell walls; they are involved in modifications of cell wall components, wall structure, signaling and interactions with plasma membrane proteins...

Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds. (2005)

Charmont, Stéphane, Jamet, Elisabeth, Pont-Lezica, Rafael, Canut, Hervé

Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical...

Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds. (2005)

Charmont, Stéphane, Jamet, Elisabeth, Pont-Lezica, Rafael, Canut, Hervé

Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical...

Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds. (2005)

Charmont, Stéphane, Jamet, Elisabeth, Pont-Lezica, Rafael, Canut, Hervé

Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical...

Cell wall proteins in apoplastic fluids of Arabidopsis thaliana rosettes: identification by mass spectrometry and bioinformatics. (2005)

Boudart, Georges, Jamet, Elisabeth, Rossignol, Michel, Lafitte, Claude, Borderies, Gisèle, Jauneau, Alain, ...

Weakly bound cell wall proteins of Arabidopsis thaliana were identified using a proteomic and bioinformatic approach. An efficient protocol of extraction based on vacuum-infiltration of the tissues...

Cell wall proteins in apoplastic fluids of Arabidopsis thaliana rosettes: identification by mass spectrometry and bioinformatics. (2005)

Boudart, Georges, Jamet, Elisabeth, Rossignol, Michel, Lafitte, Claude, Borderies, Gisèle, Jauneau, Alain, ...

Weakly bound cell wall proteins of Arabidopsis thaliana were identified using a proteomic and bioinformatic approach. An efficient protocol of extraction based on vacuum-infiltration of the tissues...

Proteomic analysis of secreted proteins from Arabidopsis thaliana seedlings: improved recovery following removal of phenolic compounds. (2005)

Charmont, Stéphane, Jamet, Elisabeth, Pont-Lezica, Rafael, Canut, Hervé

Arabidopsis thaliana seedlings grown in liquid culture were used to recover proteins secreted from the whole plant. The aim was to identify apoplastic proteins that may be lost during classical...

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts (2005)

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...

The Nicotiana sylvestris extensin gene, Ext 1.2A, is expressed in the root transition zone and upon wounding. (2004)

Guzzardi, Patricia, Genot, Geneviève, Jamet, Elisabeth

The Ext 1.2A gene of Nicotiana sylvestris L. encoding an extensin, a cell wall structural protein, was characterized. Ext 1.2A encodes a polypeptide of 311 amino acids having a highly repetitive...

Bioinformatics as a critical prerequisite to transcriptome and proteome studies. (2004)

Jamet, Elisabeth

Large-scale genomic studies rely strongly on annotations available in databases to design experimental supports such as arrays or to explain results in term of biological meaning. Most of this...

The Nicotiana sylvestris extensin gene, Ext 1.2A, is expressed in the root transition zone and upon wounding. (2004)

Guzzardi, Patricia, Genot, Geneviève, Jamet, Elisabeth

The Ext 1.2A gene of Nicotiana sylvestris L. encoding an extensin, a cell wall structural protein, was characterized. Ext 1.2A encodes a polypeptide of 311 amino acids having a highly repetitive...

Bioinformatics as a critical prerequisite to transcriptome and proteome studies. (2004)

Jamet, Elisabeth

Large-scale genomic studies rely strongly on annotations available in databases to design experimental supports such as arrays or to explain results in term of biological meaning. Most of this...

Bioinformatics as a critical prerequisite to transcriptome and proteome studies (2004)

Jamet, Elisabeth

Large-scale genomic studies rely strongly on annotations available in databases to design experimental supports such as arrays or to explain results in term of biological meaning. Most of this...

Bioinformatics as a critical prerequisite to transcriptome and proteome studies (2004)

Jamet, Elisabeth

Large-scale genomic studies rely strongly on annotations available in databases to design experimental supports such as arrays or to explain results in term of biological meaning. Most of this...

Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis. (2003)

Borderies, Gisèle, Jamet, Elisabeth, Lafitte, Claude, Rossignol, Michel, Jauneau, Alain, Boudart, Georges, ...

The complete sequencing of the Arabidopsis thaliana genome allows the use of the recently developed mass spectrometry techniques to identify the cell wall proteins (CWPs). Most proteomic approaches...

The tobacco Ext 1.4 extensin gene family is not regulated by auxins in cells proliferating under hormone control (2003)

Salva, Isabelle, Jamet, Elisabeth

It was previously shown that the tobacco Ext 1.4 extensin gene is expressed in cells proliferating under hormone control and during lateral root initiation. Now, the regulation of expression of the...

The tobacco Ext 1.4 extensin gene family is not regulated by auxins in cells proliferating under hormone control (2003)

Salva, Isabelle, Jamet, Elisabeth

It was previously shown that the tobacco Ext 1.4 extensin gene is expressed in cells proliferating under hormone control and during lateral root initiation. Now, the regulation of expression of the...

Proteomics of loosely bound cell wall proteins of Arabidopsis thaliana cell suspension cultures: a critical analysis. (2003)

Borderies, Gisèle, Jamet, Elisabeth, Lafitte, Claude, Rossignol, Michel, Jauneau, Alain, Boudart, Georges, ...

The complete sequencing of the Arabidopsis thaliana genome allows the use of the recently developed mass spectrometry techniques to identify the cell wall proteins (CWPs). Most proteomic approaches...

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...

GeneFarm, structural and functional annotation of Arabidopsis gene and protein families by a network of experts

Aubourg, Sébastien, Brunaud, Véronique, Bruyère, Clémence, Cock, Mark, Cooke, Richard, Cottet, Annick, ...

Genomic projects heavily depend on genome annotations and are limited by the current deficiencies in the published predictions of gene structure and function. It follows that, improved annotation...