Elizabeth L. Hartland

Characterisation of atypical enteropathogenic E. colistrains of clinical origin (2009)

Tennant, Sharon M, Tauschek, Marija, Azzopardi, Kristy, Bigham, Andrea, Bennett-Wood, Vicki, Hartland, Elizabeth L, ...

Abstract Background Enteropathogenic E. coli (EPEC) is a prominent cause of diarrhoea, and is characterised in part by its carriage of a pathogenicity island: the locus for enterocyte effacement...

Secretion of flagellin by the LEE-encoded type III secretion system of enteropathogenic Escherichia coli (2009)

Badea, Luminita, Beatson, Scott A, Kaparakis, Maria, Ferrero, Richard L, Hartland, Elizabeth L

Abstract Background Enteropathogenic Escherichia coli (EPEC) is an attaching and effacing (A/E) pathogen that possesses a type III secretion system (T3SS) encoded within the locus of enterocyte...

Immune Response to Infection with Mycobacterium ulcerans

Gooding, Travis M., Johnson, Paul D. R., Campbell, Dianne E., Hayman, John A., Hartland, Elizabeth L., Kemp, Andrew S., ...

Mycobacterium ulcerans is a slow-growing, acid-fast bacillus that causes chronic necrotizing skin ulcers known as Buruli ulcers. Previously reported information on immunity to this mycobacterium is...

Expression of Intimin γ from Enterohemorrhagic Escherichia coli in Citrobacter rodentium

Hartland, Elizabeth L., Huter, Veronika, Higgins, Lisa M., Goncalves, Nathalie S., Dougan, Gordon, Phillips, Alan D., ...

The carboxy-terminal 280 amino acids (Int280) of the bacterial adhesion molecule intimin include the receptor-binding domain. At least five different types of Int280, designated α, β, γ, δ, and...

Coiled-Coil Domain of Enteropathogenic Escherichia coli Type III Secreted Protein EspD Is Involved in EspA Filament-Mediated Cell Attachment and Hemolysis

Daniell, Sarah J., Delahay, Robin M., Shaw, Robert K., Hartland, Elizabeth L., Pallen, Mark J., Booy, Frank, ...

Many animal and plant pathogens use type III secretion systems to secrete key virulence factors, some directly into the host cell cytosol. However, the basis for such protein translocation has yet to...

Identification of a Novel Fimbrial Gene Cluster Related to Long Polar Fimbriae in Locus of Enterocyte Effacement-Negative Strains of Enterohemorrhagic Escherichia coli

Doughty, Stephen, Sloan, Joan, Bennett-Wood, Vicki, Robertson, Marcus, Robins-Browne, Roy M., Hartland, Elizabeth L.

Enterohemorrhagic Escherichia coli (EHEC) is a food-borne cause of bloody diarrhea and the hemolytic-uremic syndrome (HUS) in humans. Most strains of EHEC belong to a group of bacterial pathogens...

Transfer Region of pO113 from Enterohemorrhagic Escherichia coli: Similarity with R64 and Identification of a Novel Plasmid-Encoded Autotransporter, EpeA

Leyton, Denisse L., Sloan, Joan, Hill, Rebecca E., Doughty, Steven, Hartland, Elizabeth L.

Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the...

Contribution of Long Polar Fimbriae to the Virulence of Rabbit-Specific Enteropathogenic Escherichia coli

Newton, Hayley J., Sloan, Joan, Bennett-Wood, Vicki, Adams, Louise M., Robins-Browne, Roy M., Hartland, Elizabeth L.

Enteropathogenic Escherichia coli (EPEC) is a major of cause of diarrhea among children in developing countries. Although EPEC is a human specific pathogen, some related strains are natural pathogens...

Invasion of Epithelial Cells by Locus of Enterocyte Effacement-Negative Enterohemorrhagic Escherichia coli

Luck, Shelley N., Bennett-Wood, Vicki, Poon, Rachael, Robins-Browne, Roy M., Hartland, Elizabeth L.

The majority of enterohemorrhagic Escherichia coli (EHEC) strains associated with severe disease carry the locus of enterocyte effacement (LEE) pathogenicity island, which encodes the ability to...

Characterization of Two Non-Locus of Enterocyte Effacement-Encoded Type III-Translocated Effectors, NleC and NleD, in Attaching and Effacing Pathogens

Marchés, Olivier, Wiles, Siouxsie, Dziva, Francis, La Ragione, Roberto M., Schüller, Stephanie, Best, Angus, ...

Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are...

Identification of Legionella pneumophila-Specific Genes by Genomic Subtractive Hybridization with Legionella micdadei and Identification of lpnE, a Gene Required for Efficient Host Cell Entry †

Newton, Hayley J., Sansom, Fiona M., Bennett-Wood, Vicki, Hartland, Elizabeth L.

Legionella pneumophila is a ubiquitous environmental organism and a facultative intracellular pathogen of humans. To identify genes that may contribute to the virulence of L. pneumophila, we...

Essential Role of the Type III Secretion System Effector NleB in Colonization of Mice by Citrobacter rodentium

Kelly, Michelle, Hart, Emily, Mundy, Rosanna, Marchès, Olivier, Wiles, Siouxsie, Badea, Luminita, ...

Attaching and effacing (A/E) pathogens are a significant cause of gastrointestinal illness in humans and animals. All A/E pathogens carry a large pathogenicity island, termed the locus for enterocyte...

Immune Response to Infection with Mycobacterium ulcerans

Gooding, Travis M., Johnson, Paul D. R., Campbell, Dianne E., Hayman, John A., Hartland, Elizabeth L., Kemp, Andrew S., ...

Mycobacterium ulcerans is a slow-growing, acid-fast bacillus that causes chronic necrotizing skin ulcers known as Buruli ulcers. Previously reported information on immunity to this mycobacterium is...

Expression of Intimin γ from Enterohemorrhagic Escherichia coli in Citrobacter rodentium

Hartland, Elizabeth L., Huter, Veronika, Higgins, Lisa M., Goncalves, Nathalie S., Dougan, Gordon, Phillips, Alan D., ...

The carboxy-terminal 280 amino acids (Int280) of the bacterial adhesion molecule intimin include the receptor-binding domain. At least five different types of Int280, designated α, β, γ, δ, and...

Coiled-Coil Domain of Enteropathogenic Escherichia coli Type III Secreted Protein EspD Is Involved in EspA Filament-Mediated Cell Attachment and Hemolysis

Daniell, Sarah J., Delahay, Robin M., Shaw, Robert K., Hartland, Elizabeth L., Pallen, Mark J., Booy, Frank, ...

Many animal and plant pathogens use type III secretion systems to secrete key virulence factors, some directly into the host cell cytosol. However, the basis for such protein translocation has yet to...

Identification of a Novel Fimbrial Gene Cluster Related to Long Polar Fimbriae in Locus of Enterocyte Effacement-Negative Strains of Enterohemorrhagic Escherichia coli

Doughty, Stephen, Sloan, Joan, Bennett-Wood, Vicki, Robertson, Marcus, Robins-Browne, Roy M., Hartland, Elizabeth L.

Enterohemorrhagic Escherichia coli (EHEC) is a food-borne cause of bloody diarrhea and the hemolytic-uremic syndrome (HUS) in humans. Most strains of EHEC belong to a group of bacterial pathogens...

Transfer Region of pO113 from Enterohemorrhagic Escherichia coli: Similarity with R64 and Identification of a Novel Plasmid-Encoded Autotransporter, EpeA

Leyton, Denisse L., Sloan, Joan, Hill, Rebecca E., Doughty, Steven, Hartland, Elizabeth L.

Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the...

Contribution of Long Polar Fimbriae to the Virulence of Rabbit-Specific Enteropathogenic Escherichia coli

Newton, Hayley J., Sloan, Joan, Bennett-Wood, Vicki, Adams, Louise M., Robins-Browne, Roy M., Hartland, Elizabeth L.

Enteropathogenic Escherichia coli (EPEC) is a major of cause of diarrhea among children in developing countries. Although EPEC is a human specific pathogen, some related strains are natural pathogens...

Invasion of Epithelial Cells by Locus of Enterocyte Effacement-Negative Enterohemorrhagic Escherichia coli

Luck, Shelley N., Bennett-Wood, Vicki, Poon, Rachael, Robins-Browne, Roy M., Hartland, Elizabeth L.

The majority of enterohemorrhagic Escherichia coli (EHEC) strains associated with severe disease carry the locus of enterocyte effacement (LEE) pathogenicity island, which encodes the ability to...

Characterization of Two Non-Locus of Enterocyte Effacement-Encoded Type III-Translocated Effectors, NleC and NleD, in Attaching and Effacing Pathogens

Marchés, Olivier, Wiles, Siouxsie, Dziva, Francis, La Ragione, Roberto M., Schüller, Stephanie, Best, Angus, ...

Intestinal colonization by enteropathogenic and enterohemorrhagic Escherichia coli requires the locus of enterocyte effacement-encoded type III secretion system. We report that NleC and NleD are...

Identification of Legionella pneumophila-Specific Genes by Genomic Subtractive Hybridization with Legionella micdadei and Identification of lpnE, a Gene Required for Efficient Host Cell Entry †

Newton, Hayley J., Sansom, Fiona M., Bennett-Wood, Vicki, Hartland, Elizabeth L.

Legionella pneumophila is a ubiquitous environmental organism and a facultative intracellular pathogen of humans. To identify genes that may contribute to the virulence of L. pneumophila, we...

Essential Role of the Type III Secretion System Effector NleB in Colonization of Mice by Citrobacter rodentium

Kelly, Michelle, Hart, Emily, Mundy, Rosanna, Marchès, Olivier, Wiles, Siouxsie, Badea, Luminita, ...

Attaching and effacing (A/E) pathogens are a significant cause of gastrointestinal illness in humans and animals. All A/E pathogens carry a large pathogenicity island, termed the locus for enterocyte...

Contribution of FliC to Epithelial Cell Invasion by Enterohemorrhagic Escherichia coli O113:H21▿

Luck, Shelley N., Badea, Luminita, Bennett-Wood, Vicki, Robins-Browne, Roy, Hartland, Elizabeth L.

Enterohemorrhagic Escherichia coli (EHEC) O113:H21 can invade epithelial cells. In this study, we found that invasion but not adherence was inhibited by anti-FliCH21 specific antibodies. In addition,...

Contribution of a Novel Gene, rpeA, Encoding a Putative Autotransporter Adhesin to Intestinal Colonization by Rabbit-Specific Enteropathogenic Escherichia coli▿

Leyton, Denisse L., Adams, Louise M., Kelly, Michelle, Sloan, Joan, Tauschek, Marija, Robins-Browne, Roy M., ...

Rabbit-specific enteropathogenic Escherichia coli (REPEC) is an attaching and effacing pathogen of young rabbits. Using signature-tagged mutagenesis, we identified several known colonization factors...

Sel1 Repeat Protein LpnE Is a Legionella pneumophila Virulence Determinant That Influences Vacuolar Trafficking▿

Newton, Hayley J., Sansom, Fiona M., Dao, Jenny, McAlister, Adrian D., Sloan, Joan, Cianciotto, Nicholas P., ...

The environmental pathogen Legionella pneumophila possesses five proteins with Sel1 repeats (SLRs) from the tetratricopeptide repeat protein family. Three of these proteins, LpnE, EnhC, and LidL,...

The NleE/OspZ Family of Effector Proteins Is Required for Polymorphonuclear Transepithelial Migration, a Characteristic Shared by Enteropathogenic Escherichia coli and Shigella flexneri Infections▿

Zurawski, Daniel V., Mumy, Karen L., Badea, Luminita, Prentice, Julia A., Hartland, Elizabeth L., McCormick, Beth A., ...

Enteropathogenic Escherichia coli (EPEC) and Shigella flexneri are human host-specific pathogens that infect intestinal epithelial cells. However, each bacterial species employs a different infection...

Influence of Gastric Acid on Susceptibility to Infection with Ingested Bacterial Pathogens▿

Tennant, Sharon M., Hartland, Elizabeth L., Phumoonna, Tongted, Lyras, Dena, Rood, Julian I., Robins-Browne, Roy M., ...

Despite the widely held belief that gastric acid serves as a barrier to bacterial pathogens, there are almost no experimental data to support this hypothesis. We have developed a mouse model to...

Significant Role for ladC in Initiation of Legionella pneumophila Infection▿

Newton, Hayley J., Sansom, Fiona M., Dao, Jenny, Cazalet, Christel, Bruggemann, Holger, Albert-Weissenberger, Christiane, ...

Previously, we identified ladC in a cohort of genes that were present in Legionella pneumophila but absent in other Legionella species. Here we constructed a ladC mutant of L. pneumophila and...

The Major Surface-Associated Saccharides of Klebsiella pneumoniae Contribute to Host Cell Association

Clements, Abigail, Gaboriaud, Fabien, Duval, Jérôme F. L., Farn, Jacinta L., Jenney, Adam W., Lithgow, Trevor, ...

Analysing the pathogenic mechanisms of a bacterium requires an understanding of the composition of the bacterial cell surface. The bacterial surface provides the first barrier against innate immune...

Enzymatic Properties of an Ecto-nucleoside Triphosphate Diphosphohydrolase from Legionella pneumophila: SUBSTRATE SPECIFICITY AND REQUIREMENT FOR VIRULENCE*

Sansom, Fiona M., Riedmaier, Patrice, Newton, Hayley J., Dunstone, Michelle A., Müller, Christa E., Stephan, Holger, ...

Legionella pneumophila is the predominant cause of Legionnaires disease, a severe and potentially fatal form of pneumonia. Recently, we identified an ecto-nucleoside triphosphate diphosphohydrolase...

RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium▿

Hart, Emily, Yang, Ji, Tauschek, Marija, Kelly, Michelle, Wakefield, Matthew J., Frankel, Gad, ...

Citrobacter rodentium is an attaching and effacing pathogen which causes transmissible colonic hyperplasia in mice. Infection with C. rodentium serves as a model for infection of humans with...

Shiga Toxin–producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement

Newton, Hayley J., Sloan, Joan, Bulach, Dieter M., Seemann, Torsten, Allison, Cody C., Tauschek, Marija, ...

The ehx plasmids of these strains are highly related, which suggests acquisition of the large plasmid was central to the strains’ emergence.

Contribution of the pst-phoU Operon to Cell Adherence by Atypical Enteropathogenic Escherichia coli and Virulence of Citrobacter rodentium▿

Cheng, Catherine, Tennant, Sharon M., Azzopardi, Kristy I., Bennett-Wood, Vicki, Hartland, Elizabeth L., Robins-Browne, Roy M., ...

Strains of enteropathogenic Escherichia coli (EPEC) generally employ the adhesins bundle-forming pili (Bfp) and intimin to colonize the intestine. Atypical EPEC strains possess intimin but are...