Elizabeth M. J. Gillam

Quantitative whole-cell cytochrome P450 measurement suitable for high-throughput application (2008)

Johnston, Wayne A., Huang, Weiliang, De Voss, James J., Hayes, Martin A., Gillam, Elizabeth M.J.

The recombinant expression of cytochrome P450 enzymes involved in drug metabolism is of interest to the pharmaceutical and biotechnological industries due to the versatile catalytic properties of...

Direct electrochemistry of human and rat NADPH cytochrome P450 reductase (2007)

Alka Shukla, Gillam, Elizabeth M.J., Bernhardt, Paul V.

Direct electrochemistry of human and rat NADPH cytochrome P450 reductase (2006)

Shukla, Alka, Gillam, Elizabeth M. J., Bernhardt, Paul V.

The diflavo-protein NADPH cytochrome P450 reductase (CPR) is the key electron transfer partner for all drug metabolizing cytochrome P450 enzymes in humans. The protein delivers, consecutively, two...

Direct electrochemistry of human and rat NADPH cytochrome P450 reductase (2006)

Shukla, Alka, Gillam, Elizabeth M. J., Bernhardt, Paul V.

The diflavo-protein NADPH cytochrome P450 reductase (CPR) is the key electron transfer partner for all drug metabolizing cytochrome P450 enzymes in humans. The protein delivers, consecutively, two...

Role of glutamic acid 216 in cytochrome P450 2D6 substrate binding and catalysis (2003)

Guengerich, F. Peter, Hanna, Imad H., Martin, Martha V., Gillam, Elizabeth M. J.

Human cytochrome P450 (P450) 2D6 is an important enzyme involved in the metabolism of drugs, many of which are amines or contain other basic nitrogen atoms. Asp301 has generally been considered to be...

Role of glutamic acid 216 in cytochrome P450 2D6 substrate binding and catalysis (2003)

Guengerich, F. Peter, Hanna, Imad H., Martin, Martha V., Gillam, Elizabeth M. J.

Human cytochrome P450 (P450) 2D6 is an important enzyme involved in the metabolism of drugs, many of which are amines or contain other basic nitrogen atoms. Asp301 has generally been considered to be...

Roles of NADPH-P450 reductase in the O-Deethylation of 7-Ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli (2000)

Shimada, Tsutomu, Tsumura, Fujiko, Gillam, Elizabeth M. J., Guengerich, F. Peter, Inoue, Kiyoshi

Roles of NADPH-P450 reductase in the O-Deethylation of 7-Ethoxycoumarin by recombinant human cytochrome P450 1B1 variants in Escherichia coli (2000)

Shimada, Tsutomu, Tsumura, Fujiko, Gillam, Elizabeth M. J., Guengerich, F. Peter, Inoue, Kiyoshi

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M. J., ...

Metabolism of Benzo(a)pyrene to trans-7,8Dihydroxy-7,8-dihydrobenzo(a)pyrene by Recombinant Human Cytochrome P450 1B1 and Purified Liver Epoxide Hydrolase (1999)

Shimada, Tsutomu, Gillam, Elizabeth M. J., Oda, Yoshimitsu, Tsumura, Fujiko, Sutter, Thomas R., Guengerich, F. Peter, ...

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M. J., ...

Metabolism of Benzo(a)pyrene to trans-7,8Dihydroxy-7,8-dihydrobenzo(a)pyrene by Recombinant Human Cytochrome P450 1B1 and Purified Liver Epoxide Hydrolase (1999)

Shimada, Tsutomu, Gillam, Elizabeth M. J., Oda, Yoshimitsu, Tsumura, Fujiko, Sutter, Thomas R., Guengerich, F. Peter, ...

Catalytic properties of polymorphic human cytochrome P450 1B1 variants (1999)

Shimada, Tsutomu, Watanabe, Junko, Kawajiri, Kaname, Sutter, Thomas R., Guengerich, F. Peter, Gillam, Elizabeth M.J., ...

Four polymorphic human cytochrome P450 (CYP) 1B1 allelic variants, namely Arg48,Ala119,Leu432,Asn453, Arg48,Ser119,Leu432,Asn453, Arg48,Ala119,Val432,Asn-453 and Arg48,Ser119,Val432,Asn453, were...

Activation of procarcinogens by human cytochrome P450 enzymes expressed in Escherichia coli. Simplified bacterial systems for genotoxicity assays (1994)

Shimada, Tsutomu, Gillam, Elizabeth M.J., Sandhu, Punam, Guo, Zuyu, Tukey, Robert H., Guengerich, F.Peter

Bacterial assays were used to examine the activation of 14 known procarcinogens by cytochrome P450 (P450) enzymest†. Human P450s 1A1, 1A2 and 3A4 were expressed in Escherichia coil with slight...

An evaluation of potential mechanism-based inactivation of human drug metabolizing cytochromes P450 by monoamine oxidase inhibitors, including isoniazid

Polasek, Thomas M, Elliot, David J, Somogyi, Andrew A, Gillam, Elizabeth M J, Lewis, Benjamin C, Miners, John O

Identification of the human cytochromes P450 catalysing the rate-limiting pathways of gliclazide elimination

Elliot, David J, Lewis, Benjamin C, Gillam, Elizabeth M J, Birkett, Donald J, Gross, Annette S, ...

What is already known about this subjectGliclazide is a widely used oral hypoglycaemic agent.The major metabolites of gliclazide formed in vivo have been identified.However, the cytochrome P450...