Emmanuel Lacroix

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination (2005)

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells (2003)

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site‐ specific DNA double‐strand breaks...

Computational estimation of specific side chain interaction energies in {{alpha}} helices (2001)

Fisinger, Samo, Serrano, Luis, Lacroix, Emmanuel

We have used a structure energy-based computer program developed for protein design, Perla, to provide theoretical estimates of all specific side chain–side chain interaction energies occurring in...

Combinatorial approaches: A new tool to search for highly structured β-hairpin peptides

Pastor, Maria Teresa, Lacroix, Emmanuel, Serrano, Luis, Pérez-Payá, Enrique

Here we present a combinatorial approach to evolve a stable β-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a β-hairpin structure population of around...

De novo designed peptide-based amyloid fibrils

Goldie, Kenneth, Zurdo, Jesús, Lacroix, Emmanuel, Dobson, Christopher M., Hoenger, Andreas, ...

Identification of therapeutic strategies to prevent or cure diseases associated with amyloid fibril deposition in tissue (Alzheimer's disease, spongiform encephalopathies, etc.) requires a rational...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

Combinatorial approaches: A new tool to search for highly structured β-hairpin peptides

Pastor, Maria Teresa, Lacroix, Emmanuel, Serrano, Luis, Pérez-Payá, Enrique

Here we present a combinatorial approach to evolve a stable β-hairpin fold in a linear peptide. Starting with a de novo-designed linear peptide that shows a β-hairpin structure population of around...

De novo designed peptide-based amyloid fibrils

Goldie, Kenneth, Zurdo, Jesús, Lacroix, Emmanuel, Dobson, Christopher M., Hoenger, Andreas, ...

Identification of therapeutic strategies to prevent or cure diseases associated with amyloid fibril deposition in tissue (Alzheimer's disease, spongiform encephalopathies, etc.) requires a rational...

A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells

Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...

Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...

In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination

Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric

Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...

Computational estimation of specific side chain interaction energies in α helices

Fisinger, Samo, Serrano, Luis, Lacroix, Emmanuel

We have used a structure energy-based computer program developed for protein design, Perla, to provide theoretical estimates of all specific side chain–side chain interaction energies occurring in...