Linder, Bastian, Plöttner, Oliver, Kroiss, Matthias, Hartmann, Enno, Laggerbauer, Bernhard, Meister, Gunter, ...
Tudor domains are widespread among proteins involved in RNA metabolism, but only in a few cases their cellular function has been analyzed in detail. Here, we report on the characterization of the...
Dissertation zur Erlangung des akademischen Grades (2005)
Christoph Hammerschmidt, Aus Göttingen, Beda Christoph Hammerschmidt, Dekan Prof, Dr. Enno Hartmann, ...
I would like to thank my colleague Dr. Martin Kempa and my supervisor Prof. Dr. Volker Linnemann for the excellent cooperation, many helpful suggestions, and reviews. Special acknowledgments go to...
X-ray structure of a protein-conducting channel (2004)
Van Den Berg, Bert, Clemons, William M., Collinson, Ian, Modis, Yorgo, Hartmann, Enno, Harrison, Stephen C., ...
[Mikrofiche-Ausg.].
High, Stephen, Andersen, Soren S. L., Görlich, Dirk, Hartmann, Enno, Prehn, Siegfried, Rapoport, Tom A., ...
We have identified membrane components which are adjacent to type I and type II signal-anchor proteins during their insertion into the membrane of the ER. Using two different cross-linking approaches...
High, Stephen, Martoglio, Bruno, Görlich, Dirk, Andersen, Soren S. L., Ashford, Anthony J., Giner, Angelika, ...
A chemically charged amber suppressor tRNA was used to introduce the photoactivatable amino acid (Tmd)Phe at a selected position within the signal sequence of the secretory protein preprolactin. This...
Berlin, Akad. d. Wiss. d. DDR, Diss. A, 1990 (Nicht f.d. Austausch).
Görlich, Dirk, Prehn, Siegfried, Hartmann, Enno, Herz, Joachim, Otto, Albrecht, Kraft, Regine, ...
Bifunctional cross-linking reagents were used to probe the protein environment in the ER membrane of the signal sequence receptor (SSR), a 34-kD integral membrane glycoprotein (Wiedmann, M., T. V....
Structure and biosynthesis of the signal-sequence receptor (1990)
Prehn, Siegfried, Herz, Joachim, Hartmann, Enno, Kurzchalia, Teymuras V., Frank, Rainer, Römisch, Karin, ...
The signal-sequence receptor (SSR) has previously been shown to be a component of the environment which nascent polypeptides meet on passage through the endoplasmic reticulum (ER) membrane. We report...
Evidence for Distinct Substrate Specificities of Importin α Family Members in Nuclear Protein Import
Köhler, Matthias, Speck, Christian, Christiansen, Marret, Bischoff, F. Ralf, Prehn, Siegfried, Haller, Hermann, ...
Importin α plays a pivotal role in the classical nuclear protein import pathway. Importin α shuttles between nucleus and cytoplasm, binds nuclear localization signal-bearing proteins, and functions...
Jäkel, Stefan, Mingot, José-Manuel, Schwarzmaier, Petra, Hartmann, Enno, Görlich, Dirk
Many nuclear transport pathways are mediated by importin β-related transport receptors. Here, we identify human importin (Imp) 4b as well as mouse Imp4a, Imp9a and Imp9b as novel family members....
SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome
Meister, Gunter, Hannus, Stefan, Plöttner, Oliver, Baars, Tonie, Hartmann, Enno, Fakan, Stanislav, ...
SMNrp, also termed SPF30, has recently been identified in spliceosomes assembled in vitro. We have functionally characterized this protein and show that it is an essential splicing factor. We show...
Importin 13: a novel mediator of nuclear import and export
Mingot, José-Manuel, Kostka, Susanne, Kraft, Regine, Hartmann, Enno, Görlich, Dirk
Importin β-related receptors mediate translocation through nuclear pore complexes. Co-operation with the RanGTPase system allows them to bind and subsequently release their substrates on opposite...
Bohnsack, Markus T., Regener, Kathrin, Schwappach, Blanche, Saffrich, Rainer, Paraskeva, Efrosyni, Hartmann, Enno, ...
Importin β-type transport receptors mediate the vast majority of transport pathways between cell nucleus and cytoplasm. We identify here the translation elongation factor 1A (eEF1A) as the...
Prokaryotic Utilization of the Twin-Arginine Translocation Pathway: a Genomic Survey
Dilks, Kieran, Rose, R. Wesley, Hartmann, Enno, Pohlschröder, Mechthild
The twin-arginine translocation (Tat) pathway, which has been identified in plant chloroplasts and prokaryotes, allows for the secretion of folded proteins. However, the extent to which this pathway...
Use1p is a yeast SNARE protein required for retrograde traffic to the ER
Dilcher, Meik, Veith, Beate, Chidambaram, Subbulakshmi, Hartmann, Enno, Schmitt, Hans Dieter, Fischer Von Mollard, Gabriele
SNAREs on transport vesicles and target membranes are required for vesicle targeting and fusion. Here we describe a novel yeast protein with a typical SNARE motif but with low overall amino acid...
Exportin 6: a novel nuclear export receptor that is specific for profilin·actin complexes
Stüven, Theis, Hartmann, Enno, Görlich, Dirk
Active macromolecular transport between the nucleus and cytoplasm proceeds through nuclear pore complexes and is mostly mediated by transport receptors of the importin β-superfamily. Here we...
Prinz, Anke, Behrens, Christina, Rapoport, Tom A., Hartmann, Enno, Kalies, Kai-Uwe
During early stages of cotranslational protein translocation across the endoplasmic reticulum (ER) membrane the ribosome is targeted to the heterotrimeric Sec61p complex, the major component of the...
Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes
Lipowsky, Gerd, Bischoff, F.Ralf, Schwarzmaier, Petra, Kraft, Regine, Kostka, Susanne, Hartmann, Enno, ...
Transport receptors of the importin β superfamily account for many of the nuclear import and export events in eukaryotic cells. They mediate translocation through nuclear pore complexes, shuttle...
Quensel, Christina, Friedrich, Beate, Sommer, Thomas, Hartmann, Enno, Kohler, Matthias
The “classical” nuclear import pathway depends on importins α and β. Humans have only one importin β, while six α importins have been described. Whether or not distinct α importins are...
Hori, Osamu, Miyazaki, Mayuki, Tamatani, Takashi, Ozawa, Kentaro, Takano, Katsura, Okabe, Masaru, ...
Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome-associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress....
Evidence for Distinct Substrate Specificities of Importin α Family Members in Nuclear Protein Import
Köhler, Matthias, Speck, Christian, Christiansen, Marret, Bischoff, F. Ralf, Prehn, Siegfried, Haller, Hermann, ...
Importin α plays a pivotal role in the classical nuclear protein import pathway. Importin α shuttles between nucleus and cytoplasm, binds nuclear localization signal-bearing proteins, and functions...
Jäkel, Stefan, Mingot, José-Manuel, Schwarzmaier, Petra, Hartmann, Enno, Görlich, Dirk
Many nuclear transport pathways are mediated by importin β-related transport receptors. Here, we identify human importin (Imp) 4b as well as mouse Imp4a, Imp9a and Imp9b as novel family members....
SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome
Meister, Gunter, Hannus, Stefan, Plöttner, Oliver, Baars, Tonie, Hartmann, Enno, Fakan, Stanislav, ...
SMNrp, also termed SPF30, has recently been identified in spliceosomes assembled in vitro. We have functionally characterized this protein and show that it is an essential splicing factor. We show...
Importin 13: a novel mediator of nuclear import and export
Mingot, José-Manuel, Kostka, Susanne, Kraft, Regine, Hartmann, Enno, Görlich, Dirk
Importin β-related receptors mediate translocation through nuclear pore complexes. Co-operation with the RanGTPase system allows them to bind and subsequently release their substrates on opposite...
Bohnsack, Markus T., Regener, Kathrin, Schwappach, Blanche, Saffrich, Rainer, Paraskeva, Efrosyni, Hartmann, Enno, ...
Importin β-type transport receptors mediate the vast majority of transport pathways between cell nucleus and cytoplasm. We identify here the translation elongation factor 1A (eEF1A) as the...
Prokaryotic Utilization of the Twin-Arginine Translocation Pathway: a Genomic Survey
Dilks, Kieran, Rose, R. Wesley, Hartmann, Enno, Pohlschröder, Mechthild
The twin-arginine translocation (Tat) pathway, which has been identified in plant chloroplasts and prokaryotes, allows for the secretion of folded proteins. However, the extent to which this pathway...
Use1p is a yeast SNARE protein required for retrograde traffic to the ER
Dilcher, Meik, Veith, Beate, Chidambaram, Subbulakshmi, Hartmann, Enno, Schmitt, Hans Dieter, Fischer Von Mollard, Gabriele
SNAREs on transport vesicles and target membranes are required for vesicle targeting and fusion. Here we describe a novel yeast protein with a typical SNARE motif but with low overall amino acid...
Exportin 6: a novel nuclear export receptor that is specific for profilin·actin complexes
Stüven, Theis, Hartmann, Enno, Görlich, Dirk
Active macromolecular transport between the nucleus and cytoplasm proceeds through nuclear pore complexes and is mostly mediated by transport receptors of the importin β-superfamily. Here we...
Prinz, Anke, Behrens, Christina, Rapoport, Tom A., Hartmann, Enno, Kalies, Kai-Uwe
During early stages of cotranslational protein translocation across the endoplasmic reticulum (ER) membrane the ribosome is targeted to the heterotrimeric Sec61p complex, the major component of the...
Exportin 4: a mediator of a novel nuclear export pathway in higher eukaryotes
Lipowsky, Gerd, Bischoff, F.Ralf, Schwarzmaier, Petra, Kraft, Regine, Kostka, Susanne, Hartmann, Enno, ...
Transport receptors of the importin β superfamily account for many of the nuclear import and export events in eukaryotic cells. They mediate translocation through nuclear pore complexes, shuttle...
Quensel, Christina, Friedrich, Beate, Sommer, Thomas, Hartmann, Enno, Kohler, Matthias
The “classical” nuclear import pathway depends on importins α and β. Humans have only one importin β, while six α importins have been described. Whether or not distinct α importins are...
Hori, Osamu, Miyazaki, Mayuki, Tamatani, Takashi, Ozawa, Kentaro, Takano, Katsura, Okabe, Masaru, ...
Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome-associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress....
Arf1p, Chs5p and the ChAPs are required for export of specialized cargo from the Golgi
Trautwein, Mark, Schindler, Christina, Gauss, Robert, Dengjel, Jörn, Hartmann, Enno, Spang, Anne
In Saccharomyces cerevisiae, the synthesis of chitin is temporally and spatially regulated through the transport of Chs3p (chitin synthase III) to the plasma membrane in the bud neck region. Traffic...
Friedrich, Beate, Quensel, Christina, Sommer, Thomas, Hartmann, Enno, Köhler, Matthias
The “classical” nuclear protein import pathway depends on importin α and importin β. Importin α binds nuclear localization signal (NLS)-bearing proteins and functions as an adapter to access...
NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
Stavru, Fabrizia, Hülsmann, Bastian B., Spang, Anne, Hartmann, Enno, Cordes, Volker C., Görlich, Dirk
POM121 and gp210 were, until this point, the only known membrane-integral nucleoporins (Nups) of vertebrates and, thus, the only candidate anchors for nuclear pore complexes (NPCs) within the nuclear...
Kalies, Kai-Uwe, Rapoport, Tom A., Hartmann, Enno
The Sec61 complex is the central component of the protein translocation apparatus of the ER membrane. We have addressed the role of the β subunit (Sec61β) during cotranslational protein...
CRM1-mediated Recycling of Snurportin 1 to the Cytoplasm
Paraskeva, Efrosyni, Izaurralde, Elisa, Bischoff, F. Ralf, Huber, Jochen, Kutay, Ulrike, Hartmann, Enno, ...
Importin β is a major mediator of import into the cell nucleus. Importin β binds cargo molecules either directly or via two types of adapter molecules, importin α, for import of proteins with a...
A Novel Class of RanGTP Binding Proteins
Görlich, Dirk, Dabrowski, Marylena, Bischoff, F. Ralf, Kutay, Ulrike, Bork, Peer, Hartmann, Enno, ...
The importin-α/β complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. Although Ran has been implicated also in a variety of other processes,...
Yamaguchi, Atsushi, Hori, Osamu, Stern, David M., Hartmann, Enno, Ogawa, Satoshi, Tohyama, Masaya
Application of differential display to cultured rat astrocytes subjected to hypoxia allowed cloning of a novel cDNA, termed stress-associated endoplasmic reticulum protein 1 (SERP1). Expression of...