Das, L., Das, S. K., Hooberman, Barry H., Chu, Ernest H. Y., Sinsheimer, Joseph E.
Mouse lymphocytes were exposed in vitro for 2 h or in vivo for 24 h to benzidine and related aromatic amines to test for chromosome aberrations (CA) and mitotic indices. Uninduced mouse S9 was used...
Das, L., Das, S. K., Chu, Ernest H. Y., Sinsheimer, Joseph E.
Mouse lymphocytes in vivo or in vitro were exposed for 24 h to 4 aliphatic epoxides, glycidyl 1-naphthyl ether, glycidyl 4-nitrophenyl ether, 1-naphthylpropylene oxide and trichloropropylene oxide...
Single-step selection of mammalian cell mutants deficient in CTP synthetase (1989)
Li, I-Chian, Wu, Chao-Liang, Chu, Ernest H. Y.
A single-step selection of Chinese hamster V79 cells deficient in CTP synthetase (CTPS − ) is presented. The underlying principle of the direct selection is the differential and efficient killing...
Thomas, Peedikayil E., Lamb, Barbara J., Chu, Ernest H. Y.
Cytidine-triphosphate synthetase (UTP: ammonia ligase (ADP-forming), EC 6.3.4.2.) has been purified over 31 000-fold to homogeneity with 17% recovery from rat liver cytosol, using high-performance...
Li, I-Chian, Chu, Ernest H. Y.
A systematic comparison of 5 different statistical methods for the estimation of mutation rate ([mu]) in cultured Chinese hamster V79 cells is presented. Fluctuation tests were performed with several...
Chromosomal location of human genes encoding major heat-shock protein HSP70 (1987)
Drabkin, Harry A., Chu, Ernest H. Y., Kao, Fa -Ten, Hartz, Judith, Harrison, Gail Singer, Hart, Iris M., ...
The HSP70 family of heat-shock proteins constitutes the major proteins synthesized in response to elevated temperatures and other forms of stress. In eukaryotes members of the HSP70 family also...
Detection of human somatic cell structural gene mutations by two-dimensional electrophoresis (1987)
Hanash, S.M., Chu, Ernest H. Y., Kuick, Rork, Skolnick, M., Neel, J., Strahler, John R., ...
The feasibility of detecting human somatic structural gene mutations by two dimensional electrophoresis has been investigated. A lymphoblastoid cell line was grown as a mass culture in the presence...
A deterministic approach for the estimation of mutation rates in cultured mammalian cells (1985)
Li, I-Chian, Ho Wu, Shu-Chen, Fu, Jiliang, Chu, Ernest H. Y.
Unequal growth rates between mutant and wild-type cells in a large population constitute a problem for the estimation of mutation rate. Over a period of cell growth, a selective advantage of one cell...
Pleiotropic mutants of Chinese hamster cells with altered cytidine 5′-triphosphate synthetase (1984)
Li, I-Chian, Chu, Ernest H. Y., McLaren, John D., Lamb, Barbara J.
Following chemical mutagenesis and multiple-step indirect selection, four clones of Chinese hamster V79 cells were isolated which exhibited auxotrophy for thymidine, deoxycytidine, or deoxyuridine...
McLaren, John D., Chu, Ernest H. Y.
The kinetics and cytidine 5′-triphosphate (CTP) feedback inhibition of CTP synthetase in wild-type and four mutants of Chinese hamster V79 cells have been studied. The enzymes of the wild type and...
Mutants of chinese hamster cells deficient in thymidylate synthetase (1984)
Li, I-Chian, Chu, Ernest H. Y.
Stable mutants of Chinese hamster V79 cells deficient in thymidylate synthetase (TS; E.C. 2.1.1.45) have been selected from cultures grown in medium supplemented with folinic acid, aminopterin, and...
Estimation of mutation rates in cultured mammalian cells (1983)
Li, I-Chian, Fu, Jiliang, Hung, Yung-Tai, Chu, Ernest H. Y.
The factors that affect reliable estimations of mutation rates ([mu]) in cultured mammalian somatic cell populations by fluctuation analysis are studied experimentally and statistically. We analyze...
Apparent synergism between amino donors for CTP synthesis in Chinese hamster fibroblasts (1983)
McLaren, John D., Chu, Ernest H. Y.
The synergistic effects of potential amino donors were studied in the assay of CTP synthetase in extracts of Chinese hamster fibroblasts. We found that L-glutamine was not effective as the sole amino...
Apparent synergism between amino donors for CTP synthesis in Chinese hamster fibroblasts (1983)
Chu, Ernest H. Y., McLaren, John D.
The synergistic effects of potential amino donors were studied in the assay of CTP synthetase in extracts of Chinese hamster fibroblasts. We found that L-glutamine was not effective as the sole amino...
Fu, Jiliang, Li, I-Chian, Chu, Ernest H. Y.
Peer Reviewed
Modification of cell membrane in variants of chinese hamster cells resistant to abrin (1980)
Li, I-Chian, Blake, Diane A., Goldstein, Irwin J., Chu, Ernest H. Y.
Stable and heritable variants of Chinese hamster ovary (CHO) cells which are resistant to different levels (0.1, 1.0 and 10 [mu]g/ml) of the toxin abrin have been isolated and characterized. The...
MorenoFuenmayor, H., Meilinger, Karen L., Rucknagel, Donald L., Mohrenweiser, H. L., Chu, Ernest H. Y.
We report a family in which six individuals were carriers of a translocation between chromosomes 8 and 12. The balanced carriers had a chromosome constitution: 46,XX or 46,XY,t(8;12)(021;p13). Six...
Wade, M. H., Chu, Ernest H. Y.
The cytotoxic action of physical and chemical agents on 10 skin fibroblast strains in culture derived from individuals with Cockayne's syndrome was measured in terms of colony-forming ability. As...
Bostedor, Richard, Buchsbaum, Bruce, Chu, Ernest H. Y., Whitfield, Carolyn D.
Galactokinase activity is reduced in 12 independent clones of Chinese hamster ovary cells resistant to 2-deoxygalactose. The frequency of resistant colonies is increased with chemical mutagens. The...
Meilinger, Karen L., Kelch, Robert P., Wall, Patrick M., Speert, David P., Chu, Ernest H. Y., Steffensen, Dale M.
In a newborn boy with multiple malformations, a tandem duplication was detected at the distal end of the long arm of one human chromosome 1. The Giemsa bands, 1q31 to 1q43–44, were repeated...
Sun, N. C., Chu, Ernest H. Y., Sun, C. R. Y.
In the progeny of somatic cell hybrids formed by fusion of human lymphocytes and Chinese hamster mutant cells, a single human chromosome A2 was selectively retained when grown in appropriate medium.
A rapid electrophoretic technique for human and Chinese hamster galactokinase (1975)
Sun, N. C., Chang, C. C., Chu, Ernest H. Y.
In this paper a simple direct staining method is described for the detection of the human and Chinese hamster forms of galactokinase as separated by electrophoresis.