Reverse genetics and microRNAsin zebrafish (2005)
The zebrafish (Danio rerio) has become an important genetic model organism in various research areas of modern biology. Originally, it was chosen as a vertebrate model system because its excellent...
Koudijs,MJ, Den Broeder,MJ, Keijser,A, Wienholds,Erno, Houwing,S, Van Rooijen,Ellen, ...
Proliferation is one of the basic processes that control embryogenesis. To identify factors involved in the regulation of proliferation, we performed a zebrafish genetic screen in which we used...
Marco J. Koudijs, Astrid Keijser, Erno Wienholds, Saskia Houwing, ...
Proliferation is one of the basic processes that control embryogenesis. To identify factors involved in the regulation of proliferation, we performed a zebrafish genetic screen in which we used...
Koudijs, MJ, Den Broeder, MJ, Keijser, A, Wienholds, Erno, Houwing, S, Van Rooijen, Ellen, ...
Proliferation is one of the basic processes that control embryogenesis. To identify factors involved in the regulation of proliferation, we performed a zebrafish genetic screen in which we used...
Substrate requirements for let-7 function in the developing zebrafish embryo (2004)
Kloosterman, Wigard P., Wienholds, Erno, Ketting, René F., Plasterk, Ronald H.A.
MicroRNAs (miRNAs) are involved in the regulation of gene expression at the post-transcriptional level by base pairing to the 3′-UTR (untranslated region) of mRNAs. The let-7 miRNA was first...
Efficient Target-Selected Mutagenesis in Zebrafish (2003)
Wienholds, Erno, Van Eeden, Freek, Kosters, Marit, Mudde, Josine, Plasterk, Ronald H.A., Cuppen, Edwin
One of the most powerful methods available to assign function to a gene is to inactivate or knockout the gene. Recently, we described the first target-selected knockout in zebrafish. Here, we report...
Efficient Target-Selected Mutagenesis in Zebrafish (2003)
Wienholds, Erno, Van Eeden, Freek, Kosters, Marit, Mudde, Josine, Plasterk, Ronald H.A., Cuppen, Edwin
One of the most powerful methods available to assign function to a gene is to inactivate or knockout the gene. Recently, we described the first target-selected knockout in zebrafish. Here, we report...
Regulated transposition of a fish transposon in the mouse germ line
Fischer, Sylvia E. J., Wienholds, Erno, Plasterk, Ronald H. A.
Tc1/mariner elements are able to transpose in species other than the host from which they were isolated. As potential vectors for insertional mutagenesis and transgenesis of the mouse, these...
Efficient Target-Selected Mutagenesis in Zebrafish
Wienholds, Erno, Van Eeden, Freek, Kosters, Marit, Mudde, Josine, Plasterk, Ronald H.A., Cuppen, Edwin
One of the most powerful methods available to assign function to a gene is to inactivate or knockout the gene. Recently,we described the first target-selected knockout in zebrafish. Here,we report on...
Substrate requirements for let-7 function in the developing zebrafish embryo
Kloosterman, Wigard P., Wienholds, Erno, Ketting, René F., Plasterk, Ronald H.A.
MicroRNAs (miRNAs) are involved in the regulation of gene expression at the post-transcriptional level by base pairing to the 3′-UTR (untranslated region) of mRNAs. The let-7 miRNA was first...
tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors
Berghmans, Stéphane, Murphey, Ryan D., Wienholds, Erno, Neuberg, Donna, Kutok, Jeffery L., Fletcher, Christopher D. M., ...
TP53 is the most frequently mutated tumor suppressor gene in human cancer, with nearly 50% of all tumors exhibiting a loss-of-function mutation. To further elucidate the genetic pathways involving...
The Zebrafish Mutants dre, uki, and lep Encode Negative Regulators of the Hedgehog Signaling Pathway
Koudijs, Marco J, Den Broeder, Marjo J., Keijser, Astrid, Wienholds, Erno, Houwing, Saskia, ...
Proliferation is one of the basic processes that control embryogenesis. To identify factors involved in the regulation of proliferation, we performed a zebrafish genetic screen in which we used...
Fischer, Sylvia E J, Wienholds, Erno, Plasterk, Ronald H A
In a genome-wide analysis of the active transposons in Caenorhabditis elegans we determined the localization and sequence of all copies of each of the six active transposon families. Most copies of...
Regulated transposition of a fish transposon in the mouse germ line
Fischer, Sylvia E. J., Wienholds, Erno, Plasterk, Ronald H. A.
Tc1/mariner elements are able to transpose in species other than the host from which they were isolated. As potential vectors for insertional mutagenesis and transgenesis of the mouse, these...
Efficient Target-Selected Mutagenesis in Zebrafish
Wienholds, Erno, Van Eeden, Freek, Kosters, Marit, Mudde, Josine, Plasterk, Ronald H.A., Cuppen, Edwin
One of the most powerful methods available to assign function to a gene is to inactivate or knockout the gene. Recently,we described the first target-selected knockout in zebrafish. Here,we report on...
Substrate requirements for let-7 function in the developing zebrafish embryo
Kloosterman, Wigard P., Wienholds, Erno, Ketting, René F., Plasterk, Ronald H.A.
MicroRNAs (miRNAs) are involved in the regulation of gene expression at the post-transcriptional level by base pairing to the 3′-UTR (untranslated region) of mRNAs. The let-7 miRNA was first...
tp53 mutant zebrafish develop malignant peripheral nerve sheath tumors
Berghmans, Stéphane, Murphey, Ryan D., Wienholds, Erno, Neuberg, Donna, Kutok, Jeffery L., Fletcher, Christopher D. M., ...
TP53 is the most frequently mutated tumor suppressor gene in human cancer, with nearly 50% of all tumors exhibiting a loss-of-function mutation. To further elucidate the genetic pathways involving...
The Zebrafish Mutants dre, uki, and lep Encode Negative Regulators of the Hedgehog Signaling Pathway
Koudijs, Marco J, Den Broeder, Marjo J., Keijser, Astrid, Wienholds, Erno, Houwing, Saskia, ...
Proliferation is one of the basic processes that control embryogenesis. To identify factors involved in the regulation of proliferation, we performed a zebrafish genetic screen in which we used...
Fischer, Sylvia E J, Wienholds, Erno, Plasterk, Ronald H A
In a genome-wide analysis of the active transposons in Caenorhabditis elegans we determined the localization and sequence of all copies of each of the six active transposon families. Most copies of...
T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine
Muncan, Vanesa, Faro, Ana, Haramis, Anna-Pavlina G, Hurlstone, Adam F L, Wienholds, Erno, Van Es, Johan, ...
Previous studies have shown that Wnt signals, relayed through β-catenin and T-cell factor 4 (Tcf4), are essential for the induction and maintenance of crypts in mice. We have now generated a tcf4...