Etienne Thiry

Coinfection with two closely related alphaherpesviruses results in a highly diversified recombination mosaic displaying negative genetic interference (2009)

Muylkens, Benoît, Farnir, Frédéric, Meurens, François, Schynts, Frédéric, Vanderplasschen, Alain, Georges, Michel, ...

Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of...

Coinfection with two closely related alphaherpesviruses results in a highly diversified recombination mosaic displaying negative genetic interference (2009)

Muylkens, Benoît, Farnir, Frédéric, Meurens, François, Schynts, Frédéric, Vanderplasschen, Alain, Georges, Michel, ...

Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of...

Identification of equid herpesvirus-5 in respiratory liquids: A retrospective study of 785 samples taken in 2006-2007 (2009)

Fortier, G, Pronost, S, Miszczak, F, Fortier, C, Léon, A, Richard, B, ...

During a case control study undertaken in 2006-2007, a screening and consensus polymerase chain reaction (PCR) was performed to evaluate the potential role of equid herpesviruses (EHV) in several...

The order Herpesvirales. (2009)

Davison, Andrew J, Eberle, Richard, Ehlers, Bernhard, Hayward, Gary S, McGeoch, Duncan J, Minson, Anthony C, ...

The taxonomy of herpesviruses has been updated by the International Committee on Taxonomy of Viruses (ICTV). The former family Herpesviridae has been split into three families, which have been...

Serological Evidence of Caprine Herpesvirus 1 Infection in Mediterranean France (2008)

Thiry, Julien, Saegerman, Claude, Chartier, C, Mercier, P, Keuser, V, Thiry, Etienne

Caprine herpesvirus 1 (CpHV-1) is responsible of a systemic disease in kids and genital diseases inducing abortions in adult goats. In Europe, CpHV-1 is widespread in Mediterranean countries such as...

Animal Noroviruses (2008)

Scipioni, Alexandra, Mauroy, Axel, Vinje, Jan, Thiry, Etienne

Among enteric caliciviruses, noroviruses belong to the genus Norovirus, one of the four accepted genera in the family Caliciviridae. These single-stranded, positive-sense RNA viruses are highly...

Infection humaine par le virus B du singe en Afrique (2008)

Nsabimana, Jean-Marie Mafuko, Moutschen, Michel, Thiry, Etienne, Meurens, Francois

Simian herpes B virus or Cercopithecine herpesvirus 1 (CeHV-1) is enzootic (80% to 100%) in Asian monkeys of the genus Macaca but is also present in other monkey species. This virus, discovered in...

Atypical myopathy in grazing horses: A first exploratory data analysis. (2008)

Votion, Dominique, Linden, Annick, Delguste, Catherine, Amory, Hélène, Thiry, Etienne, Engels, Patrick, ...

Over the last decade, atypical myopathy (AM) in grazing horses has emerged in several European countries. An exploratory analysis was conducted to determine horse- and pasture-level indicators or...

Detection and quantification of human and bovine novoviruses by a TaqMan RT-PCR assay with a control for inhibition. (2008)

Scipioni, Alexandra, Bourgot, Isabelle, Mauroy, Axel, Ziant, Dominique, Saegerman, Claude, Daube, Georges, ...

Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and calves. Reverse...

Experimental reactivation of equine herpesvirus-3 following corticosteroid treatment. (2008)

Barrandeguy, M, Vissani, A, Olguin, C, Becerra, L, Mino, S, Pereda, A, ...

State of latency, well known for several herpesviruses, has been proposed for equine herpesvirus-3 (EHV-3) and supported by epidemiological observations. No detailed assessment about reactivation,...

Noroviruses and sapoviruses in pigs in Belgium. (2008)

Mauroy, Axel, Scipioni, Alexandra, Mathijs, Elisabeth, Miry, Cora, Ziant, Dominique, Thys, Christine, ...

Porcine noroviruses and sapoviruses belong to the family Caliciviridae and are rarely reported in European countries. In this study, swine stools from a region representative of northern Europe were...

A SYBR Green RT-PCR assay in single tube to detect human and bovine noroviruses and control for inhibition. (2008)

Scipioni, Alexandra, Mauroy, Axel, Ziant, Dominique, Saegerman, Claude, Thiry, Etienne

BACKGROUND: Noroviruses are single-stranded RNA viruses belonging to the family Caliciviridae. They are a major cause of epidemic and sporadic gastroenteritis in humans and clinical signs and lesions...

Animal noroviruses. (2008)

Scipioni, Alexandra, Mauroy, Axel, Vinje, J, Thiry, Etienne

Among enteric caliciviruses, noroviruses belong to the genus Norovirus, one of the four accepted genera in the family Caliciviridae. These single-stranded, positive-sense RNA viruses are highly...

Atypical myopathy in grazing horses: A first exploratory data analysis. (2008)

Votion, Dominique, Linden, Annick, Delguste, Catherine, Amory, Hélène, Thiry, Etienne, Engels, Patrick, ...

Over the last decade, atypical myopathy (AM) in grazing horses has emerged in several European countries. An exploratory analysis was conducted to determine horse- and pasture-level indicators or...

Bluetongue in captive yaks. (2008)

Mauroy, Axel, Guyot, Hugues, De Clercq, Kris, Cassart, Dominique, Thiry, Etienne, Saegerman, Claude

In August 2006, several Northern European countries including Belgium reported their first cases of bluetongue (BT). Surprisingly, it was the first time that BT was diagnosed so far in the northern...

Experimental reproduction of bluetongue virus serotype 8 clinical disease in calves. (2008)

Dal Pozzo, Fabiana, De Clercq, K., Guyot, Hugues, Vandemeulebroucke, E., Sarradin, P., Vandenbussche, F., ...

Cattle are commonly subclinically infected following natural or experimental infection with bluetongue virus (BTV). The introduction of BTV serotype 8 (BTV-8) in Europe has been characterized by the...

Infection humaine par le virus B du singe en Afrique. (2008)

Mafuko Nsabimana, Jean-Marie, Moutschen, Michel, Thiry, Etienne, Meurens, François

Simian herpes B virus or Cercopithecine herpesvirus 1 (CeHV-1) is enzootic (80% to 100%) in Asian monkeys of the genus Macaca but is also present in other monkey species. This virus, discovered in...

Concepts in the prevention of bovine respiratory disease. (2008)

Makoschey, B, Lekeux, Pierre, Lacroux, C, Taylor, G, Hodgson, C, Letellier, C, ...

The bovine respiratory disease (BRD) complex requires further research both, to fully understand the disease from the different perspectives as well as to develop new tools and strategies for...

Marker vaccines and the impact of their use on diagnosis and prophylactic measures (2007)

Vannier, P, Capua, I, Le Potier, MF, Mackay, DKJ, Muylkens, Benoît, Parida, S, ...

Molecular biology and technical advances in DNA recombination have ushered in a new era in vaccinology. This article examines the recent development of specific marker vaccines and examines the...

Molecular epidemiology of canine parvovirus, Europe (2007)

Decaro, N, Desario, C, Addie, DD, Martella, V, Vieira, MJ, Elia, G, ...

Canine parvovirus (CPV), which causes hemorrhagic enteritis in dogs, has 3 antigenic variants: types 2a, 2b, and 2c. Molecular method assessment of the distribution of the CPV variants in Europe...

Vaccination guidelines: a bridge between official requirements and the daily use of vaccines (2007)

Thiry, Etienne, Horzinek, MC

Vaccination guidelines are non-compulsory recommendations which assist the veterinary practitioner to use vaccines efficiently. They complement the official information contained in the shortened...

Cidofovir is effective against caprine herpesvirus 1 infection in goats (2007)

Tempesta, M, Camero, M, Bellacicco, AL, Thiry, Julien, Crescenzo, G, Neyts, J, ...

Caprine herpesvirus 1 (CpHV-1) is a virus able to cause genital infection leading to vulvovaginitis or balanoposthitis in adult goats. CpHV-1 shares several biological similarities with herpes...

Highly pathogenic avian influenza H5N1 virus in cats and other carnivores (2007)

Thiry, Etienne, Zicola, Angélique, Addie, D, Egberink, H, Hartmann, K, Lutz, H, ...

The Asian lineage highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen of birds. Only recently, the virus has been reported to cause sporadic fatal disease in carnivores, and its...

Bluetongue in Belgium, 2006 (2007)

Toussaint, J. F., Sailleau, C., Mast, J., Houdart, P., Czaplicki, G., Demeestere, L., ...

Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription-quantitative PCRs (RT-qPCRs) that amplified...

Sanitary control in bovine embryo transfer - How far should we go? A review (2007)

Van Soom, A, Imberechts, H, Delahaut, P, Thiry, Etienne, Van Roy, V, Walravens, K, ...

Embryo transfer is a globally executed technique which, when properly done, has both economic and sanitary advantages. International guidelines are available to prevent infection of the embryo with...

Cidofovir is effective against caprine herpesvirus 1 infection in goats (2007)

Camero, Michele, Bellacicco, Anna Lucia, Thiry, Julien, Crescenzo, Giuseppe, ...

Caprine herpesvirus 1 (CpHV-1) is a virus able to cause genital infection leading to vulvovaginitis or balanoposthitis in adult goats. CpHV-1 shares several biological similarities with herpes...

Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis (2007)

Muylkens, Benoît, Thiry, Julien, Kirten, P, Schynts, F, Thiry, Etienne

Bovine herpesvirus 1 (BoHV-1), classified as an alphaherpesvirus, is a major pathogen of cattle. Primary infection is accompanied by various clinical manifestations such as infectious bovine...

Clinical Protection against Caprine Herpesvirus 1 Genital Infection by Intranasal Administration of a Live Attenuated Glycoprotein E Negative Bovine Herpesvirus 1 Vaccine (2007)

Thiry, Julien, Tempesta, M, Camero, M, Tarsitano, E, Muylkens, Benoît, Meurens, F, ...

BACKGROUND: Caprine herpesvirus 1 (CpHV-1) is responsible of systemic diseases in kids and genital diseases leading to abortions in goats. CpHV-1 is widespread and especially in Mediterranean...

Feline herpesvirus (2007)

Gaskell, R, Dawson, S, Radford, A, Thiry, Etienne

Feline herpesvirus (FHV-1; felid herpesvirus 1 (FeHV-1)) is an alphaherpesvirus of cats closely related to canine herpesvirus-1 and phocine herpesvirus-1. There is only one serotype of the virus and...

History and clinical features of atypical myopathy in horses in Belgium (2000-2005) (2007)

Votion, DM, Linden, Annick, Saegerman, Claude, Engels, Patrick, Erpicum, Michel, Thiry, Etienne, ...

Background: The emergent nature of atypical myopathy or atypical myoglobinuria (AM) necessitates precise description of its clinical and epidemiologic features. Purpose: To define key features of AM...

Isolation and Characterisation of a Ruminant Alphaherpesvirus Closely Related to Bovine Herpesvirus 1 in a Free-Ranging Red Deer (2007)

Thiry, Julien, Widen, F, Grégoire, Fabien, Linden, Annick, Belak, S, Thiry, Etienne

BACKGROUND: The genus Varicellovirus of the Herpesviridae subfamily Alphaherpesvirinae includes a cluster of viruses antigenically and genetically related to bovine herpesvirus 1 (BoHV-1): namely...

Potent inhibition of genital herpesvirus infection in goats by cidofovir (2007)

Tempesta, M, Camero, M, Bellacicco, AL, Tarsitano, E, Crescenzo, G, Thiry, Julien, ...

Background: Like human herpesvirus 2 (HHV-2) in humans, infection by caprine herpesvirus 1 in goats is associated with genital lesions, and this provides a unique model to study the efficacy and...

Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle (2006)

Muylkens, Benoît, Meurens, F, Schynts, F, Farnir, Frédéric, Pourchet, A, Bardiau, Marjorie, ...

Vaccines used in control programmes of Bovine herpesvirus 1 (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are...

Biological characterization of bovine herpesvirus 1 recombinants possessing the vaccine glycoprotein E negative phenotype (2006)

Muylkens, Benoît, Meurens, F, Schynts, F, De Fays, K, Pourchet, A, Thiry, Julien, ...

Intramolecular recombination is a frequent event during the replication cycle of bovine herpesvirus 1 (BoHV-1). Recombinant viruses frequently arise and survive in cattle after concomitant nasal...

Recombination in the alphaherpesvirus bovine herpesvirus 1 (2006)

Thiry, Etienne, Muylkens, Benoît, Meurens, F, Gogev, S, Thiry, Julien, Vanderplasschen, Alain, ...

Herpesviruses are DNA viruses characterized by a low rate of nucleotide substitution. Therefore. other mechanisms must be involved to their evolution, like recombination that can be seen as an...

Characterization of caprine herpesvirus 1 glycoprotein D gene and its translation product (2006)

Keuser, Véronique, Detry, Bruno, Thiry, Julien, De Fays, K, Schynts, F, Pastoret, Paul-Pierre, ...

Caprine herpesvirus 1 (CpHV- 1) is responsible of systemic infection in neonatal kids as well as abortion and fertility disorders in adult goats. This virus is closely related to bovine herpesvirus 1...

Descriptive and spatial epidemiology of Rift valley fever outbreak in Yemen 2000-2001 (2006)

Abdo-Salem, S, Gerbier, G, Bonnet, P., Al-Qadasi, M, Tran, A, Thiry, Etienne, ...

Rift valley fever (RVF) is an arboviral disease produced by a bunyavirus belonging to the genus Phlebovirus. Several species of Aedes and Culex are the vectors of this virus that affects sheep,...

Ruminant alphaherpesviruses related to bovine herpesvirus 1 (2006)

Thiry, Julien, Keuser, V, Muylkens, Benoît, Meurens, F, Gogev, S, Vanderplasschen, Alain, ...

Herpesviruses have mainly co-evolved with their hosts for millions of years. Consequently, different related host species may have been infected by various genetically related herpesviruses....

Ruminant alphaherpesviruses related to bovine herpesvirus 1. (2006)

Thiry, Julien, Keuser, Veronique, Muylkens, Benoît, Meurens, Francois, Gogev, Sacha, Vanderplasschen, Alain, ...

Herpesviruses have mainly co-evolved with their hosts for millions of years. Consequently, different related host species may have been infected by various genetically related herpesviruses....

Analysis of antibody response in goats to caprine herpesvirus 1 (2005)

Tempesta, M, Greco, G, Tarsitano, E, Thiry, Julien, Camero, M, Decaro, N, ...

Serum samples of goats experiencing natural and experimental infections and/or reactivation of caprine herpesvirus 1 (CpHV.1) were analysed with neutralization and Western blotting (WB) tests. WB...

Antibodies against bovine herpesvirus 4 are highly prevalent in wild African buffaloes throughout eastern and southern Africa (2005)

Dewals, Benjamin J, Gillet, Louis, Gerdes, T, Taracha, EL, Thiry, Etienne, Vanderplasschen, Alain

Bovine herpesvirus 4 (BoHV-4) has been isolated from cattle throughout the world. Interestingly, a survey of wild African buffaloes mainly from the Maasai Mara Game Reserve in Kenya revealed that 94%...

Inhibition of bovine sperm-zona binding by bovine herpesvirus-1 (2005)

Tanghe, S, Vanroose, G, Van Soom, A, Duchateau, L, Ysebaert, MT, Kerkhofs, P, ...

The purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (BoHV-1) with sperm-oocyte interactions during bovine in vitro fertilization. An inhibition of almost...

Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny (2005)

Dehan, Pierre, Couvreur, B, Hamers, C, Lewalle, P, Thiry, Etienne, Kerkhofs, P, ...

An infectious cDNA clone of the hypervirulent bovine viral diarrhoea virus (BVDV) strain 890 (isolate 256) was produced by a streamlined PCR procedure. As compared to the published sequence of strain...

Canine herpesvirus-1 (CHV-1): clinical, serological and virological patterns in breeding colonies (2005)

Ronsse, V, Verstegen, J, Thiry, Etienne, Onclin, K, Aeberle, C, Brunet, Sébastien, ...

Canine herpesvirus-1 (CHV-1) is presumed to be enzootic in the dog population and is associated with reproductive disorders and neonatal mortality. To advise dog breeders towards an effective...

Population-level retrospective study of neurologically expressed disorders in ruminants before the onset of bovine spongiform encephalopathy (BSE) in Belgium, a BSE risk III country (2005)

Saegerman, Claude, Berkvens, D, Claes, L, Dewaele, Albert, Coignoul, Freddy, Ducatelle, R, ...

A retrospective epidemiological study (n = 7,875) of neurologically expressed disorders (NED) in ruminants before the onset of the bovine spongiform encephalopathy epidemic (years studied, 1980 to...

Recombination in alphaherpesviruses (2005)

Thiry, Etienne, Meurens, F, Muylkens, Benoît, McVoy, M, Gogev, S, Thiry, Julien, ...

Within the Herpesviridae family, Alphaherpesvirinae is an extensive subfamily which contains numerous mammalian and avian viruses. Given the low rate of herpesvirus nucleotide substitution,...

Interspecific recombination between two ruminant alphaherpesviruses, bovine herpesviruses 1 and 5 (2004)

Meurens, F, Keil, GM, Muylkens, Benoît, Gogev, S, Schynts, F, Negro, S, ...

Homologous recombination between different species of alphaherpesviruses has been described between herpes simplex viruses 1 and 2 but has not yet been observed between other alphaherpesviruses. In...

Glycol chitosan improves the efficacy of intranasally administrated replication defective human adenovirus type 5 expressing glycoprotein D of bovine herpesvirus 1 (2004)

Gogev, S, De Fays, K, Versali, Marie-France, Gautier, S, Thiry, Etienne

The ability of two soluble formulations, namely chitosan and glycol chitosan, when used as an intranasal adjuvant, to improve the immunogenicity of an intranasal human adenovirus type 5 replication...

Superinfection prevents recombination of the alphaherpesvirus bovine herpesvirus 1 (2004)

Meurens, F, Schynts, F, Keil, GM, Muylkens, Benoît, Vanderplasschen, Alain, Gallego, P, ...

Homologous recombination between strains of the same alphaherpesvirus species occurs frequently both in vitro and in vivo. This process has been described between strains of herpes simplex virus type...

Isolation of caprine herpesvirus type 1 in Spain (2004)

Keuser, V, Espejo-Serrano, J, Schynts, F, Georgin, JP, Thiry, Etienne

Two strains of caprine herpesvirus type 1 (CpHV-1) were isolated after the experimental reactivation of two seropositive goats in Spain. Viral DNA from these isolates was compared with DNA from...

Improved antigenic methods for differential diagnosis of bovine, caprine, and cervine alphaherpesviruses related to bovine herpesvirus 1 (2004)

Keuser, V, Schynts, F, Detry, Bruno, Mathieu, Anne, Robert, B, Vanderplasschen, Alain, ...

The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four...

Investigation of the susceptibility of human cell lines to bovine herpesvirus 4 infection: Demonstration that human cells can support a nonpermissive persistent infection which protects them against tumor necrosis factor alpha-induced apoptosis (2004)

Gillet, Laurent, Minner, F, Detry, Bruno, Farnir, Frédéric, Willems, Luc, Lambot, M, ...

Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus that has a worldwide distribution in the population of cattle. Many factors make human contamination by BoHV-4 likely to occur. In this study, we...

Decision support tools for clinical diagnosis of disease in cows with suspected bovine spongiform encephalopathy (2004)

Saegerman, Claude, Speybroeck, N, Roels, S, Vanopdenbosch, E, Thiry, Etienne, Berkvens, D

Reporting of clinically suspected cattle is currently the most common method for detecting cases of bovine spongiform encephalopathy (BSE). Improvement of clinical diagnosis and decision-making...

Hospital outbreak of gastroenteritis due to norovirus in Belgium (2004)

Verbelen, V, Bodeus, Monique, Garrino, MG, Scipioni, Alexandra, Kabambal, B, Daube, Georges, ...

We report an outbreak of gastroenteritis due to Norovirus in a care unit in a Belgian hospital involving thirty-three people. The origin of the outbreak was traced to one nursing assistant. The virus...

Bovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5 (2004)

Gogev, S, Georgin, JP, Schynts, F, Vanderplasschen, Alain, Thiry, Etienne

Bovine herpesvirus 1 glycoprotein D (gD) gene expression by recombinant replication defective human adenovirus type 5 (HAdV-5) was investigated in calves using indirect immunofluorescence microscopy...

Risk evaluation of the transmission of the avian influenza virus to humans (2004)

Saegerman, Claude, Meulemans, G, Van Reeth, K, Marlier, Didier, Yane, F, Vindevogel, Henri, ...

Since mid-december 2003, an epizootic of highly pathogenic avian influenza (type A, sub-type H5N1) occurs in eastern and south-eastern Asia. This epizootic is historically unprecedented in its...

Decision support tools for clinical diagnosis of disease in cows with suspected bovine spongiform encephalopathy. (2004)

Saegerman, Claude, Speybroeck, N, Roels, S, Vanopdenbosch, E, Thiry, Etienne, Berkvens, D

Reporting of clinically suspected cattle is currently the most common method for detecting cases of bovine spongiform encephalopathy (BSE). Improvement of clinical diagnosis and decision-making...

Deux protocoles d’hyperimmunisation au moyen de vaccins marqués réduisent l’incidence de séroconversion envers l’herpèsvirus bovin 1 en cheptels laitiers : résultats d’une étude sur le terrain (2004)

Dispas, M, Lemaire, Mylène, Speybroeck, N, Berkvens, D, Merveille, Anne, Boelaert, F, ...

A 28 months long cohort study was organized in dairy herds in Belgium, to assess the efficacy of hyperimmunisation as a tool for the control of infectious bovine rhinotracheitis. Two protocols of...

Biosafety of Herpesvirus Vectors (2003)

Gogev, S, Schynts, F, Meurens, F, Bourgot, I, Thiry, Etienne

Herpesviruses are large DNA viruses, which possess a number of advantages as gene delivery vectors. These relate to an ability to package large DNA insertions and establish lifelong latent infections...

Rise and survival of bovine herpesvirus 1 recombinants after primary infection and reactivation from latency (2003)

Schynts, F, Meurens, F, Detry, Bruno, Vanderplasschen, Alain, Thiry, Etienne

Recombination is thought to be an important source of genetic variation in herpesviruses. Several studies, performed in vitro or in vivo, detected recombinant viruses after the coinoculation of two...

The structures of bovine herpesvirus 1 virion and concatemeric DNA: implications for cleavage and packaging of herpesvirus genomes (2003)

Schynts, F, McVoy, MA, Meurens, F, Detry, Bruno, Epstein, AL, Thiry, Etienne

Herpesvirus genomes are often characterized by the presence of direct and inverted repeats that delineate their grouping into six structural classes. Class D genomes consist of a long (L) segment and...

Isolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field (2003)

Dispas, M, Schynts, F, Lemaire, Mylène, Letellier, C, Vanopdenbosch, E, Thiry, Etienne, ...

During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal...

L’herpèsvirus bovin 4 (2003)

Markine-Goriaynoff, N, Minner, F, De Fays, K, Gillet, Louis, Thiry, Etienne, Pastoret, Paul-Pierre, ...

Bovine herpesvirus 4 (BoHV-4) belongs to the Herpesviridae family, Gammaherpesvirinae subfamily, Rhadinovirus genus like human herpesvirus 8 (HHV-8), the causative agent of Kaposi's sarcoma. BoHV-4...

L’herpèsvirose canine (2003)

Ronsse, V, Poulet, H, Verstegen, J, Thiry, Etienne

Canine herpesvirus is known to be a causal agent of reproduction disorders and diseases of the upper respiratory system. Subclinical infections are also frequently observed. The interest for the...

Les chitosanes – nouveaux adjuvants pour la vaccination par voie muqueuse chez les animaux (2003)

Gogev, S, Versali, Marie-France, Thiry, Etienne

The advantage of mucosal vaccination is the induction of an immune response at entry sites of pathogens. Because vaccines alone are poorly bioavailable after mucosal administration, they need to be...

Induction of protective immunity to bovine herpesvirus type 1 in cattle by intranasal administration of replication-defective human adenovirus type 5 expressing glycoprotein gC or gD (2002)

Gogev, S, Vanderheijden, N, Lemaire, Mylène, Schynts, F, D'Offay, J, Deprez, I, ...

Replication-defective human adenoviruses type 5 (HAd5) expressing the bovine herpesvirus type 1 (BHV-1) glycoprotein gC or gD under the control of the human cytomegalovirus immediate-early...

Advantages and disadvantages of a control programme against infectious bovine rhinotracheitis in Belgium (2002)

Limbourg, B, Kerkhofs, P, Massard, C, Michelet, S, Saegerman, Claude, Thiry, Etienne

A working party of the "Association d'Epidemiologie et de Sante Animale" assessed the strategy for a control of infectious bovine rhinotracheitis (IBR) in Belgium and studied its feasability and the...

L’herpèsvirus B du singe, un agent d’anthropozoonose méconnu (2002)

Meurens, F, Gallego, P, Bourgot, I, Thiry, Etienne

B-virus or Cercopithecine herpesvirus 1 (CeHV-1) is a zoonotic alphaherpesvirus enzootic in Asian monkeys of the genus Macaca that is genetically and antigenically closely related to the human...

Caractéristiques du système immunitaire du fœtus bovin et du veau nouveau-né. Implications dans la prévention et le diagnostic des infections d’origine virale (2002)

Thiry, Etienne, Schynts, F, Lemaire, Mylène

In this review the characteristics of the immune system of the bovine fetus and neonate are presented. These characteristics are important for the prevention and diagnosis of viral infections. Three...

Stratégies de prévention des avortements provoqués par les herpèsvirus et les pestivirus de ruminants (2002)

Thiry, Etienne

Bovine herpesviruses 1 and 4 (BoHV-1 and BoHV-4) and caprine herpesvirus (CpHV-1) are responsible of abortion. Abortions caused by BoHV-1 happen during outbreaks of infectious bovine rhinotracheitis...

Establishment of Latency Associated with Glycoprotein E (Ge) Seroconversion after Bovine Herpesvirus 1 Infection in Calves with High Levels of Passive Antibodies Lacking Ge Antibodies (2001)

Schynts, F, Lemaire, Mylène, Ros, C, Belak, S, Thiry, Etienne

This study was conducted to investigate the glycoprotein E (gE) antibody response raised after inoculation with a low infectious dose of bovine herpesvirus 1 (BHV-1) in six calves possessing high...

Latency and Reactivation of a Glycoprotein E Negative Bovine Herpesvirus Type 1 Vaccine: Influence of Virus Load and Effect of Specific Maternal Antibodies (2001)

Lemaire, Mylène, Schynts, Frédéric, Meyer, Gilles, Georgin, Jean-Pierre, Baranowski, Eric, Gabriel, Annick, ...

The effects of the vaccination of neonatal calves with a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV-1) were investigated in naive and passively immunised calves either with the...

Use of PCR and Immunofluorescence to Detect Bovine Herpesvirus 1 Recombinants (2001)

Schynts, F, Vanderplasschen, Alain, Hanon, E, Rijsewijk, F A, Van Oirschot, J T, Thiry, Etienne

Homologous recombination occurs frequently between strains of the same alphaherpesvirus species. Studies of this phenomenon require techniques that can differentiate parental strains from putative...

Cross-Sectional Study of the Association between Pathological Conditions and Myxoma-Virus Seroprevalence in Intensive Rabbit Farms in Europe (2001)

Marlier, Didier, Herbots, J, Detilleux, Johann, Lemaire, Michelle, Thiry, Etienne, Vindevogel, Henri

Myxomatosis is a major viral disease of the European rabbit (Oryctolagus cuniculus). Two forms of the disease (nodular and amyxomatous) exist. The clinical diagnosis of the nodular form is easily...

Human viral infections derived from food (2001)

Scipioni, Alexandra, Daube, Georges, Thiry, Etienne

Several viruses may be transmitted to humans via food including hepatitis A and E virus (VHA and VHE), Norwalk type calicivirus, rotavirus and astrovirus. Enterovirus and enteric adenovirus may also...

Comparative Pathogenesis of Acute and Latent Infections of Calves with Bovine Herpesvirus Types 1 and 5 (2001)

Meyer, Gilles, Lemaire, Mylène, Ros, C, Belak, K, Gabriel, Annick, Cassart, Dominique, ...

This study was conducted to compare the pathogenesis of acute and latent infections with closely related bovine herpesvirus types 1 (BHV-1) and 5 (BHV-5) in their natural host. Two groups of eight...

Specific Passive Immunity Reduces the Excretion of Glycoprotein E-Negative Bovine Herpesvirus Type 1 Vaccine Strain in Calves (2000)

Lemaire, Mylène, Hanon, E, Schynts, Frédéric, Meyer, Gilles, Thiry, Etienne

We investigated the excretion of either a glycoprotein E (gE)-negative bovine herpesvirus type 1 (BHV1) vaccine strain or a conventional modified-live vaccine strain in both naive and passively...

Production of Bovine Herpesvirus Type 1-Seronegative Latent Carriers by Administration of a Live-Attenuated Vaccine in Passively Immunized Calves (2000)

Lemaire, Mylène, Meyer, Gilles, Baranowski, Eric, Schynts, Frédéric, Wellemans, Guy, Kerkhofs, Pierre, ...

The consequences of the vaccination of neonatal calves with the widely used live-attenuated temperature-sensitive (ts) bovine herpesvirus type 1 (BHV-1) were investigated. The ts strain established...

A Multipotential Beta -1,6-N-Acetylglucosaminyl-Transferase Is Encoded by Bovine Herpesvirus Type 4 (2000)

Vanderplasschen, Alain, Markine-Goriaynoff, N, Lomonte, P, Suzuki, M, Hiraoka, N, Yeh, JC, ...

The beta-1,6-N-acetylglucosaminyltransferase (beta1,6GnT) gene family encodes enzymes playing crucial roles in glycan synthesis. Important changes in beta1,6GnT expression are observed during...

Effects of Bovine Herpesvirus Type 1 Infection in Calves with Maternal Antibodies on Immune Response and Virus Latency (2000)

Lemaire, Mylène, Weynants, Vincent, Godfroid, Jacques, Schynts, Frédéric, Meyer, Gilles, Letesson, Jean-Jacques, ...

The presence of maternally derived antibodies can interfere with the development of an active antibody response to antigen. Infection of seven passively immunized young calves with a virulent strain...

Identification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products (1999)

Meyer, Gilles, Bare, O, Thiry, Etienne

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding...

Channel Catfish Virus Gene 50 Encodes a Secreted, Mucin-Like Glycoprotein (1999)

Vanderheijden, N, Hanson, L A, Thiry, Etienne, Martial, J A

Cells infected with the wild-type (WT) strain of channel catfish virus (CCV) secreted a glycoprotein with an apparent molecular mass (MM) superior to 200 kDa into the culture medium. This protein,...

A Specific Pcr to Differentiate between Ge Negative Vaccine and Wildtype Bovine Herpesvirus Type 1 Strains (1999)

Schynts, F., Baranowski, E., Lemaire, Mylène, Thiry, Etienne

In the context of infectious bovine rhinotracheitis (IBR) control programmes using glycoprotein E (gE) deleted marker vaccines, a PCR assay was developed to allow the genotypic differentiation...

Antibody Response to Glycoprotein E after Bovine Herpesvirus Type 1 Infection in Passively Immunised, Glycoprotein E-Negative Calves (1999)

Lemaire, Mylène, Schynts, Frédérick, Meyer, Gilles, Thiry, Etienne

This study was conducted to determine whether young calves with maternal antibodies against bovine herpesvirus type 1 (BHV-1) but without antibodies against glycoprotein E (gE) can produce an active...

Attachment but Not Penetration of Bovine Herpesvirus 1 Is Necessary to Induce Apoptosis in Target Cells (1998)

Hanon, Emmanuel, Meyer, Gilles, Vanderplasschen, Alain, Dessy-Doize, Cécile, Thiry, Etienne, Pastoret, Paul-Pierre

Bovine herpesvirus 1 (BHV-1) induces apoptotic cell death in bovine peripheral blood mononuclear cells and B-lymphoma cells. Using a BHV-1 glycoprotein H null mutant, we have demonstrated that...

Bovine Herpesvirus Type 1 Glycoprotein H Is Essential for Penetration and Propagation in Cell Culture (1998)

Meyer, G., Hanon, E, Georlette, D, Pastoret, Paul-Pierre, Thiry, Etienne

Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL. To study the role of BHV-1 gH in the virus infectious cycle,...

Humoral and Cell-Mediated Immune Responses of Beef and Dairy Cattle Experimentally Infested with Psoroptes Ovis (1998)

Lonneux, J F, Nguyen, T Q, Hollanders, W, Denis, M, Thiry, Etienne, Pastoret, Paul-Pierre, ...

OBJECTIVE: To compare cellular and humoral immune responses of beef (Belgian White and Blue [BWB]) and dairy (Friesian-Holstein [FH]) cattle to Psoroptes ovis infestation and to determine whether P...

Virulence, immunogenicity and reactivation of bovine herpesvirus 1 mutants with a deletion in the gC, gG, gI, gE, or in both the gI and gE gene. (1998)

Kaashoek, M J, Rijsewijk, F A, Ruuls, R C, Keil, G M, Thiry, Etienne, Pastoret, Paul-Pierre, ...

Within the framework of developing a marker vaccine against bovine herpesvirus 1 (BHV1), several mutants with deletions in non-essential glycoprotein genes were constructed. Glycoprotein gC, gG, gI...

An association exists between bovine herpesvirus-4 seropositivity and abortion in cows. (1998)

Czaplicki, G, Thiry, Etienne

The prevalence of cattle seropositive to bovine herpesvirus-4 (BHV-4) is high in Belgium. In Belgian farms, clinical signs associated with BHV-4 infection essentially involve the genital tract and...

Analysis of the Biochemical Properties of, and Complex Formation between, Glycoproteins H and L of the Gamma2 Herpesvirus Bovine Herpesvirus-4 (1997)

Lomonte, P, Filée, Patrice, Lyaku, J R, Bublot, M, Pastoret, Paul-Pierre, Thiry, Etienne

Genes encoding glycoprotein gH and gL homologues were localized in the genome of the gamma-herpesvirus bovine herpesvirus-4 (BHV-4). Both genes were sequenced and glutathione S-transferase fusion...

Glycoprotein B of Bovine Herpesvirus 4 Is a Major Component of the Virion, Unlike That of Two Other Gammaherpesviruses, Epstein-Barr Virus and Murine Gammaherpesvirus 68 (1997)

Lomonte, P, Filée, Patrice, Lyaku, J R, Bublot, M, Pastoret, Paul-Pierre, Thiry, Etienne

This study reports that in bovine herpesvirus 4, glycoprotein B (gB) is a heterodimer and a major component of the virion, unlike gBs of Epstein-Barr virus (gp110) and murine gammaherpesvirus 68, two...

Sequence Analysis of the Bovine Herpesvirus Type 1 Genes Homologous to the DNA Polymerase (Ul30), the Major DNA-Binding Protein (Ul29) and Icp18.5 Assembly Protein (Ul28) Genes of Herpes Simplex Virus (1997)

Meyer, Gilles, Vlcek, C, Paces, V, O'Hara, M K, Pastoret, Paul-Pierre, Thiry, Etienne, ...

The nucleotide sequence of a 10.5 kb region (map position 0.332 to 0.410) of bovine herpesvirus type 1 (BHV-1) was determined. This region contained three open reading frames (ORFs) homologous to...

Bovine Herpesvirus 4: Genomic Organization and Relationship with Two Other Gammaherpesviruses, Epstein-Barr Virus and Herpesvirus Saimiri (1996)

Lomonte, P, Bublot, M, Van Santen, V, Keil, G, Pastoret, Paul-Pierre, Thiry, Etienne

Bovine herpesvirus 4 (BHV-4) belongs to the gammaherpesvirinae subfamily. Although the whole sequence of BHV-4 genome is not known it was possible, based on random sequencing, to assume that its...

Structural and Functional Analysis of Bovine Herpesvirus 1 Minor Glycoproteins (1996)

Baranowski, Eric, Keil, Günther, Lyaku, Japhet, Rijsewijk, Frans A. M., Van Oirschot, Jan T., Pastoret, Paul-Pierre, ...

This paper focuses on the structure and functions of bovine herpesvirus 1 minor glycoproteins gH, gE, gG and gp42. It reviews the progress which has been made in their identification and...

The Role of Glycoproteins gC, gE, gI and gG in the Induction of Cell-Mediated Immune Responses to Bovine Herpesvirus 1 (1996)

Denis, M, Hanon, E, Rijsewijk, F A, Kaashoek, MJ, Van Oirschot, J T, Thiry, Etienne, ...

Mutant viruses with deletions in genes encoding non-essential glycoproteins are considered as promising bovine herpesvirus 1 (BHV1) vaccine candidates. The present study compared the influence of...

Establishment of a Rabbit Model for Bovine Herpesvirus Type 5 Neurological Acute Infection (1996)

Meyer, Gilles, Lemaire, Mylène, Lyaku, J, Pastoret, Paul-Pierre, Thiry, Etienne

This study was conducted to evaluate the suitability of the rabbit as a model for bovine, herpesvirus 5 (BHV-5) acute infection. In a preliminary experiment, a total of 24 one-month old New Zealand...

Assessment of the cell-mediated immunity in cattle infection after bovine herpesvirus 4 infection, using an in vitro antigen-specific interferon-gamma assay. (1996)

Godfroid, J, Czaplicki, G, Kerkhofs, P, Weynants, V, Wellemans, G, Thiry, Etienne, ...

The cell-mediated immunity (CMI) following bovine herpesvirus 4 (BHV4) infection has been poorly investigated in cattle. The in vivo response measured by a delayed type of hypersensitivity (DTH)...

Glycoprotein H (gII/gp108) and glycoprotein L form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1. (1996)

Khattar, S., Tikoo, S. K., Babiuk, L. A., Baranowski, E., Plainchamp, D., ...

The glycoproteins of bovine herpesvirus 1 (BHV-1) play important roles in the interactions between virions and target cells. A 108 kDa glycoprotein, designated gII or gp 108, has been identified by...

The Replication in Vitro of the Gammaherpesvirus Bovine Herpesvirus 4 Is Restricted by Its DNA Synthesis Dependence on the S Phase of the Cell Cycle (1995)

Vanderplasschen, Alain, Goltz, M, Lyaku, J, Benarafa, C, Buhk, H J, Thiry, Etienne, ...

Because several observations have suggested that replication of the gammaherpesvirus bovine herpesvirus 4 (BHV-4) is influenced by the physiological state of the host cell, a study was carried out to...

Analysis of Bovine Herpesvirus 4 Genomic Regions Located Outside the Conserved Gammaherpesvirus Gene Blocks (1995)

Lomonte, P, Bublot, M, Van Santen, V, Keil, G M, Pastoret, Paul-Pierre, Thiry, Etienne

Bovine herpesvirus 4 (BHV-4) DNA sequences located outside the gene blocks conserved among the gammaherpesviruses BHV-4, herpesvirus saimiri (HVS) and Epstein-Barr virus (EBV) were analysed. Twelve...

Synthesis and Processing of Bovine Herpesvirus-1 Glycoprotein H (1995)

Baranowski, E, Dubuisson, J, Babiuk, A L, Michel, A, Pastoret, Paul-Pierre, ...

The translation product of the bovine herpesvirus-1 (BHV-1) gH gene was identified and characterized. Synthetic peptides were used to generate specific antisera and a glycoprotein of 108K was...

Quantitative Assessment of the Specific Cd4+ T Lymphocyte Proliferative Response in Bovine Herpesvirus 1 Immune Cattle (1994)

Denis, M, Kaashoek, MJ, Van Oirschot, J T, Pastoret, Paul-Pierre, Thiry, Etienne

We quantified the CD4+ T cell proliferation specific for bovine herpesvirus 1 (BHV-1) in peripheral blood mononuclear cells from cattle. The stimulation index as detected in proliferative assays...

Epidemiological evaluation of a monoclonal ELISA detecting antibodies against bovine leukaemia virus in serum pools. (1994)

Knapen, K, Kerkhofs, P, Thiry, Etienne, Mammerickx, M

Sensitivity, specificity and predictive values of an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against bovine leukaemia virus (BLV) were evaluated using a representative...

Attachment of the Gammaherpesvirus Bovine Herpesvirus 4 Is Mediated by the Interaction of Gp8 Glycoprotein with Heparinlike Moieties on the Cell Surface (1993)

Vanderplasschen, Alain, Bublot, M, Dubuisson, J, Pastoret, Paul-Pierre, Thiry, Etienne

Cell surface heparan sulfate serves as the initial receptor for several alphaherpesviruses and at least one betaherpesvirus. This study shows that during the process of adsorption of the...

Identification of Different Target Glycoproteins for Bovine Herpes Virus Type 1-Specific Cytotoxic T Lymphocytes Depending on the Method of in Vitro Stimulation (1993)

Denis, M., Slaoui, M., Keil, G., Babiuk, L. A., Ernst, E., Pastoret, Paul-Pierre, ...

Vaccinia virus recombinants expressing the three major bovine herpes virus-1 (BHV-1) glycoproteins gI, gIII and gIV were used to identify the major target antigens for BHV-1-specific CTL isolated...

Identification of 108k, 93k, and 42k Glycoproteins of Bovine Herpesvirus-1 by Monoclonal Antibodies (1993)

Baranowski, E., Dubuisson, J., Pastoret, Paul-Pierre, Thiry, Etienne

Three glycoproteins of bovine herpesvirus-1 (BHV-1) other than glycoproteins gI, gIII, and gIV were identified by monoclonal antibody (MAb) analyses. Monoclonal antibodies were obtained by...

Restriction Maps of the DNA of Cervid Herpesvirus 1 and Cervid Herpesvirus 2, Two Viruses Related to Bovine Herpesvirus 1 (1993)

Vanderplasschen, Alain, Bublot, M, Pastoret, Paul-Pierre, Thiry, Etienne

Restriction maps of cervid herpesviruses 1 and 2 which are antigenetically related to bovine herpesvirus 1, were deduced from Southern blot hybridization with HindIII restriction fragments of BHV-1...

Molecular Biology of Bovine Herpesvirus Type 4 (1992)

Thiry, Etienne, Bublot, M, Dubuisson, J, Van Bressem, M F, Lequarré, Anne-Sophie, Lomonte, P, ...

Bovine herpesvirus type 4 (BHV-4) is a ubiquitous virus of cattle. Its genome is a 144 +/- 6 kb double-stranded DNA consisting of a unique central part (L-DNA) flanked at both ends by tandem repeats...

Epidemiological Evaluation of a Monoclonal Elisa Detecting Bovine Viral Diarrhoea Pestivirus Antigens in Field Blood Samples of Persistently Infected Cattle (1992)

Mignon, Bernard, Waxweiler, Sophie, Thiry, Etienne, Boulanger, D, Dubuisson, J, Pastoret, Paul-Pierre

An enzyme-linked immunosorbent assay (ELISA), using monoclonal antibodies for capture and detection, was developed for detecting bovine viral diarrhoea virus (BVDV) antigens in blood samples. The...

Genetic Relationships between Bovine Herpesvirus 4 and the Gammaherpesviruses Epstein-Barr Virus and Herpesvirus Saimiri (1992)

Bublot, M, Lomonte, P, Lequarré, Anne-Sophie, Albrecht, JC, Nicholas, J, Fleckenstein, B, ...

The overall arrangement of genes in the unique central part of the bovine herpesvirus type 4 (BHV-4) genome has been deduced by analysis of short DNA sequences. Twenty-three genes conserved in at...

Identification and Characterization of Glycoprotein Gp1 of Bovine Herpesvirus Type 4 (1992)

Dubuisson, J, Pastoret, Paul-Pierre, Thiry, Etienne

Three major bovine herpesvirus type 4 (BHV-4) glycoproteins have been described previously. By using monoclonal antibodies produced against BHV-4 envelope proteins from which the three major antigens...

Effect of Repeated Intradermal Injections of Bovine Herpesvirus Type 1 Antigen on Seronegative Cattle (1992)

Thiry, Etienne, Wellemans, G, Limbourg, B, Broes, A, Pastoret, Paul-Pierre

Forty-three cattle seronegative to bovine herpesvirus-1 (BHV-1) were given from one to five intradermal injections of BHV-1 inactivated antigen at four-week intervals. This delayed hypersensitivity...

Proteins of Bovine Herpesvirus Type 4 Released into the Culture Medium of Productively Infected Cells: Identification of a 135k Glycoprotein Involved in Viral Attachment (1992)

Dubuisson, J, Koromyslov, I, Pastoret, Paul-Pierre, Thiry, Etienne

Three bovine herpesvirus type 4 (BHV-4) proteins released into the culture medium of infected cells were identified, with Mr values of 135K, 16K and 14.5K. Among these three proteins, two were...

Location and Characterization of the Bovine Herpesvirus Type 4 Thymidine Kinase Gene; Comparison with Thymidine Kinase Genes of Other Herpesviruses (1992)

Lomonte, P, Bublot, M, Pastoret, Paul-Pierre, Thiry, Etienne

The location and nucleotide sequence of the bovine herpesvirus type 4 (BHV-4) thymidine kinase (TK) gene was determined. The coding region of the TK gene is 1335 nucleotides long and corresponds to a...

Bovine Herpesvirus 4 Isolates: A Comparison of Three Major Glycoproteins (1991)

Dubuisson, J, Bublot, M, Wellemans, G, Pastoret, Paul-Pierre, Thiry, Etienne

Twenty-four Belgian field isolates of bovine herpesvirus 4 (BHV-4), together with four reference strains were compared by radio-immunoprecipitation and western blotting using a polyvalent antiserum...

A Mathematical Model of Rinderpest Infection in Cattle Populations (1991)

Tille, A, Lefèvre, Claude, Pastoret, Paul-Pierre, Thiry, Etienne

A mathematical model for the epidemiology of rinderpest was developed, starting from a simplified descriptive analysis of the disease. A formula for the calculation of the probability of infection of...

A Mathematical Model of Rinderpest Infection in Cattle Populations (1991)

Tille, A, Lefèvre, Claude, Pastoret, Paul-Pierre, Thiry, Etienne

A mathematical model for the epidemiology of rinderpest was developed, starting from a simplified descriptive analysis of the disease. A formula for the calculation of the probability of infection of...

Temporal Control of Bovine Herpesvirus Type 4 Glycoprotein Synthesis (1991)

Dubuisson, J, Pastoret, Paul-Pierre, Thiry, Etienne

The glycoprotein gp6/gp10/gp17, gp11/VP24 and gp8 are major bovine herpesvirus type 4 antigens. The temporal expression of these glycoproteins was studied and it was shown that gp6/gp10/gp17 appeared...

Characterization of Monoclonal Antibodies to Bovine Viral Diarrhoea Virus: Evidence of a Neutralizing Activity against Gp48 in the Presence of Goat Anti-Mouse Immunoglobulin Serum (1991)

Boulanger, D, Waxweiler, Sophie, Karelle, L, Loncar, M, Mignon, Bernard, Dubuisson, J, ...

Twenty-one monoclonal antibodies (MAbs) directed against the NY-1 and the Osloss-c strains of bovine viral diarrhoea virus were produced and characterized by indirect immunofluorescence assay,...

Comparison of Proteins of Simian Herpesvirus Aotus Type 2 and Bovine Herpesvirus Type 4 (1991)

Dubuisson, J, Danyi, Sophie, Bublot, M, Pastoret, Paul-Pierre, Thiry, Etienne

Genomes of herpesvirus aotus type 2 (HVA-2) and bovine herpesvirus type 4 (BHV-4) have previously been shown to be closely similar. Moreover, preliminary serological data indicated that HVA-2 is...

Antigenic and Genomic Identity between Simian Herpesvirus Aotus Type 2 and Bovine Herpesvirus Type 4 (1991)

Bublot, M, Dubuisson, J, Van Bressem, M F, Danyi, Sophie, Pastoret, Paul-Pierre, Thiry, Etienne

Herpesvirus aotus type 2 (HVA-2) was isolated from a culture of kidney cells from a healthy owl monkey (Aotus trivirgatus). Bovine herpesvirus type 4 (BHV-4) is frequently isolated from diseased and...

Genomic Diversity among Bovine Herpesvirus 4 Field Isolates (1991)

Bublot, M, Wellemans, G, Van Bressem, M F, Dubuisson, J, Pastoret, Paul-Pierre, Thiry, Etienne

Twenty-eight Belgian field isolates of bovine herpesvirus 4 (BHV-4) coming from a variety of clinical diseases have been studied by restriction analysis and Southern blot hybridization. The unique...

Enquete serologique sur la prevalence de l'infection par l'herpes virus bovin type 4 dans les troupeaux bovins des plaines d'Accra au Ghana. (1991)

Marchot, Philippe, Thiry, Etienne, Jetteur, P, Leroy, Pascal

Sera from cattle of the Accra Plains in Ghana were screened for bovine type-4 herpesvirus (BHV-4) antibodies by an indirect immunofluorescence test. Among the 176 screened serums, 14% were found...

Neutralization of Bovine Herpesvirus Type 4 by Pairs of Monoclonal Antibodies Raised against Two Glycoproteins and Identification of Antigenic Determinants Involved in Neutralization (1990)

Dubuisson, J, Guillaume, José, Boulanger, D, Thiry, Etienne, Bublot, M, Pastoret, Paul-Pierre

In infected cattle, bovine herpesvirus type 4 (BHV-4) induces an immune response with low neutralizing antibody levels or in the absence of such antibodies. For the study of this phenomenon,...

The Biology of Bovine Herpesvirus-4 Infection of Cattle (1990)

Thiry, Etienne, Dubuisson, J, Bublot, M, Van Bressem, M F, Pastoret, Paul-Pierre

The biology of bovine herpesvirus-4 (BHV-4) infection of cattle is reviewed. The infection is distributed worldwide. Most of isolated viruses are non-pathogenic in cattle; some of them are able to...

Bovine Herpesvirus 4 Genome: Cloning, Mapping and Strain Variation Analysis (1990)

Bublot, M, Van Bressem, M F, Thiry, Etienne, Dubuisson, J, Pastoret, Paul-Pierre

The restriction map of the bovine herpesvirus 4 (BHV-4) genome (V. Test strain) was established for the restriction enzymes EcoRI, BamHI and HindIII by analysis of clones from a lambda library...

Enquête sérologique concernant les virus IBR, CHV2, BVD, PI3, RSB et bovipestique chez les petits ruminants au Zaïre. (1990)

Jetteur, P, Thiry, Etienne, Pastoret, Paul-Pierre

More than 400 small ruminant sera from Zaire were screened for antibodies to IBR, CHV2, BVD, bovine and ovine PI3, BRS and rinderpest viruses. Sera from local animals were negative for BVD, PI3 and...

Round table on infectious bovine rhinotracheitis/infectious pustular vulvovaginitis virus infection diagnosis and control. (1990)

Ackermann, M, Belak, S, Bitsch, V, Edwards, S, Moussa, A, Rockborn, G, ...

The current situation of infectious bovine rhinotracheitis/infectious pustular vulvovaginitis infection in various European countries is reviewed. Whilst some have a high serological prevalence and...

Experimental Infection of Bulls with a Genital Isolate of Bovine Herpesvirus-4 and Reactivation of Latent Virus with Dexamethasone (1989)

Dubuisson, J, Thiry, Etienne, Bublot, M, Thomas, I, Van Bressem, M F, Coignoul, Freddy, ...

Five 13- to 18-month old Belgian Blue bulls were used in this experiment. Four bulls (Nos. 2, 3, 4 and 5) were inoculated intratesticularly with 10(5) plaque-forming units of bovine herpesvirus-4...

Proteins Specified by Bovine Herpesvirus Type 4: Structural Proteins of the Virion and Identification of Two Major Glycoproteins by Using Monoclonal Antibodies (1989)

Dubuisson, Jean, Boulanger, Denise, Bublot, Michel, Thiry, Etienne, Pastoret, Paul-Pierre

Bovine herpesvirus type 4 proteins were identified by PAGE of [35S]methionine- or [3H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain...

Production and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4 (1989)

Dubuisson, J, Thiry, Etienne, Bublot, M, Sneyers, M, Boulanger, D, Guillaume, José, ...

Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma...

Recherche des anticorps dirigés contre les BHV-1, BHV-2, BHV-4, le virus BVD-MD, les adenovirus A et B, le rotavirus et le coronavirus bovins chez des bovins de l'Ouest du Zaire: résultats complémentaires. (1989)

Eyanga, E, Jetteur, P, Thiry, Etienne, Wellemans, G, Dubuisson, J, Van Opdenbosch, E, ...

Two-hundred bovine sera from western Zaire were screened for antibodies to 8 viruses: BHV-1, BHV-2, BHV-4, BVD-MD virus, bovine adenovirus A and B, bovine rotavirus and bovine coronavirus. Positive...

Biological and Biochemical Comparison of Bovid Herpesvirus-4 Strains (1988)

Dubuisson, J, Thiry, Etienne, Thalasso, F, Bublot, M, Pastoret, Paul-Pierre

Bovid herpesvirus-4 (BHV-4) isolates V.Test and LVR140, isolated from genital disease, respectively, in bull and in cow, and the reference strains Movar 33/63 and DN599 were compared by several...

Serological survey of herpesvirus infections in wild ruminants of France and Belgium. (1988)

Thiry, Etienne, Vercouter, M, Dubuisson, J, Barrat, J, Sepulchre, C, Gerardy, Cécile, ...

The presence of antibodies against bovine herpesvirus 1 (BHV-1), bovid herpesvirus 6 (BHV-6), herpesvirus of Cervidae type 1 (HVC-1), reindeer herpesvirus, bovine herpesvirus 2 (BHV-2) and bovid...

Therapy of Aujeszky's disease (pseudorabies) in naturally infected and artificially inoculated piglets using BW B759U (9-[1,3-dihydroxy-2-propoxymethyl] guanine). (1988)

Rollinson, E A, White, G, Thiry, Etienne, Dubuisson, J, Pastoret, Paul-Pierre

Two experimental porcine models of Aujeszky's disease (AD) were compared for assessing the efficacy of potential antiviral compounds. While signs were the same following intranasal inoculation and...

Les porteurs de virus: analyse des états d'équilibre entre le virus et son hôte (1987)

Pastoret, Paul-Pierre, Thiry, Etienne, Dubuisson, J

The various mechanisms of virus persistence are described. Four kinds of virus persistence are presented, depending on their epidemiological significance, persistence associated with continuous...

Reactivation of infectious bovine rhinotracheitis virus by transport. (1987)

Thiry, Etienne, Saliki, J, Bublot, M, Pastoret, Paul-Pierre

Transport was studied as a cause of reactivation of infectious bovine rhinotracheitis virus (Bovine herpesvirus-1; BHV-1) in heifers vaccinated 2-6 months before transport, using a double dose of the...

Development of specific antibodies mediating antibody-dependent cell-mediated cytotoxicity following experimental infection of cattle with bovine herpesvirus 1 and subsequent viral reactivation. (1986)

Hanton, G, Thiry, Etienne, Brochier, B, Derboven, G, Pastoret, Paul-Pierre

Evolution of sensitizing antibodies involved in antibody-dependent cell-mediated cytotoxicity (ADCC), antibody-complement cell lysis and complement-facilitated ADCC was followed in bulls after...

Logical description of bovine herpesvirus type 1 latent infection. (1986)

Pastoret, Paul-Pierre, Thiry, Etienne, Thomas, René

Description of the interactions between bovine herpesvirus type 1 (BHV-1) and cattle was performed by the method known as kinetic logic. This logical formalization uses variables with two possible...

Diagnosis and prophylaxis of infectious bovine rhinotracheitis: the role of virus latency. (1985)

Pastoret, Paul-Pierre, Thiry, Etienne

Efficient methods of diagnosis and prophylaxis of infectious bovine rhinotracheitis must consider the concept of latency of the etiological agent, infectious bovine rhinotracheitis virus (Bovine...

Effect of dexamethasone on the mean plaque size of bovine herpesvirus 1. (1985)

Curvers, V, Thiry, Etienne, Leroy, Pascal, Kinnaert, P, Pastoret, Paul-Pierre

The effect of dexamethasone on infectious bovine rhinotracheitis virus (Bovine herpesvirus 1: BHV 1) was studied by measuring the mean plaque size produced by eight virus strains under two...

Excretion and reexcretion of thermosensitive and wild-type strains of infectious bovine rhinotracheitis virus after co-infection or two successive infections. (1985)

Thiry, Etienne, Brochier, B, Saliki, J, Pirak, M, Pastoret, Paul-Pierre

Twelve cattle were divided into 2 groups. The first was intranasally co-infected with 2 strains of infectious bovine rhinotracheitis virus (Bovine herpesvirus 1; BHV 1): the thermosensitive vaccine...

Le virus de la rhinotracheite infectieuse bovine (bovine herpesvirus 1): aspects biochimiques. (1984)

Thiry, Etienne, Pastoret, Paul-Pierre

The biochemical characteristics of infectious bovine rhinotracheitis virus (Bovine herpes-virus 1, BHV 1) are reviewed: description of the virion particle and virus purification, analysis of...

Effect of homologous delayed hypersensitivity testing on specific and non-specific lymphoblastic transformation in cattle latently infected with bovine rhinotracheitis virus (bovine herpesvirus 1, BHV 1). (1984)

Brochier, B, Thiry, Etienne, Derboven, G, Hanton, G, Pastoret, Paul-Pierre

Two cattle latently infected with infectious bovine rhinotracheitis virus (Bovine herpesvirus 1, BHV 1) were intradermally injected with inactivated BHV 1 antigen (delayed hypersensitivity test, DHT,...

In vivo and in vitro effect of acyclovir on pseudorabies virus, infectious bovine rhinotracheitis virus and pigeon herpesvirus. (1983)

Thiry, Etienne, Vindevogel, Henri, Leroy, Pascal, Pastoret, Paul-Pierre, Schwers, A, Brochier, B, ...

The effect of various concentrations of acyclovir on the mean plaque size of pseudorabies virus (SHV), infectious bovine rhinotracheitis virus (IBR virus) and pigeon herpesvirus (PHV) has been...

Enquête sérologique sur la stomatite papuleuse bovine (1981)

Aguilar-Setién, Alvaro, Pastoret, Paul-Pierre, Thiry, Etienne, Pivont, P, Gregoire, R, Antoine, H, ...

A serosurvey was made in Belgium to determine the incidence of antibodies against S.P.B. virus, in bovine sera, by immunodiffusion. The sera were taken from calves, adult bovines or extracted from...

Bluetongue in Belgium, 2006

Toussaint, Jean-François, Sailleau, Corinne, Mast, Jan, Houdart, Philippe, Czaplicki, Guy, Demeestere, Lien, ...

Bluetongue has emerged recently in Belgium. A bluetongue virus strain was isolated and characterized as serotype 8. Two new real-time reverse transcription–quantitative PCRs (RT-qPCRs) that...

Coinfection with Two Closely Related Alphaherpesviruses Results in a Highly Diversified Recombination Mosaic Displaying Negative Genetic Interference ▿

Muylkens, Benoît, Farnir, Frédéric, Meurens, François, Schynts, Frédéric, Vanderplasschen, Alain, Georges, Michel, ...

Phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. However, despite an ever-increasing amount of...