Southworth, M W, Kong, H, Kucera, R B, Ware, J, Jannasch, H W, Perler, F B
Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein splicing elements (inteins) are present in...
Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis.
Fuhrman, J A, Lane, W S, Smith, R F, Piessens, W F, Perler, F B
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model,...
Intervening sequences in an Archaea DNA polymerase gene.
Perler, F B, Comb, D G, Jack, W E, Moran, L S, Qiang, B, Kucera, R B, ...
The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous open...
Compilation and analysis of intein sequences.
Perler, F B, Olsen, G J, Adam, E
We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the...
InBase, the New England Biolabs Intein Database.
Inteins are intervening sequences that splice as proteins, not RNA. InBase, the New England Biolabs Intein Database (http://www.neb. com/neb/inteins.html), is a comprehensive on-line database that...
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
Telenti, A, Southworth, M, Alcaide, F, Daugelat, S, Jacobs, W R, Perler, F B
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial...
Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
Joshua, G W, Perler, F B, Wang, C C
In nematodes, the 22 nucleotide (nt) spliced leader (SL) is normally encoded by a multi-copy, tandemly reiterated SL gene and is trans-spliced from SL-RNA onto the 5' end of a subset of mRNAs. We...
Protein splicing removes intervening sequences in an archaea DNA polymerase.
Hodges, R A, Perler, F B, Noren, C J, Jack, W E
The Vent DNA polymerase gene from Thermococcus litoralis contains two in-frame insertions that must be spliced out to form the mature polymerase. Primer extension and cDNA PCR revealed no evidence of...
Xu, M Q, Comb, D G, Paulus, H, Noren, C J, Shao, Y, Perler, F B
Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently...
The mechanism of protein splicing and its modulation by mutation.
Protein splicing results in the expression of two mature proteins from a single gene. After synthesis of a precursor protein, an internal segment (the intein) is excised and the external domains are...
Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.
Perler, F B, Davis, E O, Dean, G E, Gimble, F S, Jack, W E, Neff, N, ...
Control of protein splicing by intein fragment reassembly.
Southworth, M W, Adam, E, Panne, D, Byer, R, Kautz, R, Perler, F B
Inteins are protein splicing elements that mediate their excision from precursor proteins and the joining of the flanking protein sequences (exteins). In this study, protein splicing was controlled...
Southworth, M W, Kong, H, Kucera, R B, Ware, J, Jannasch, H W, Perler, F B
Five extremely thermophilic Archaea from hydrothermal vents were isolated, and their DNA polymerases were cloned and expressed in Escherichia coli. Protein splicing elements (inteins) are present in...
Transmission-blocking antibodies recognize microfilarial chitinase in brugian lymphatic filariasis.
Fuhrman, J A, Lane, W S, Smith, R F, Piessens, W F, Perler, F B
Brugia malayi is a parasitic nematode that causes lymphatic filariasis in humans. The monoclonal antibody MF1, which mediates clearance of peripheral microfilaremia in a gerbil infection model,...
Intervening sequences in an Archaea DNA polymerase gene.
Perler, F B, Comb, D G, Jack, W E, Moran, L S, Qiang, B, Kucera, R B, ...
The DNA polymerase gene from the Archaea Thermococcus litoralis has been cloned and expressed in Escherichia coli. It is split by two intervening sequences (IVSs) that form one continuous open...
Compilation and analysis of intein sequences.
Perler, F B, Olsen, G J, Adam, E
We have compiled a list of all the inteins (protein splicing elements) whose sequences have been published or were available from on-line sequence databases as of September 18, 1996. Analysis of the...
InBase, the New England Biolabs Intein Database.
Inteins are intervening sequences that splice as proteins, not RNA. InBase, the New England Biolabs Intein Database (http://www.neb. com/neb/inteins.html), is a comprehensive on-line database that...
The Mycobacterium xenopi GyrA protein splicing element: characterization of a minimal intein.
Telenti, A, Southworth, M, Alcaide, F, Daugelat, S, Jacobs, W R, Perler, F B
The 198-amino-acid in-frame insertion in the gyrA gene of Mycobacterium xenopi is the smallest known naturally occurring active protein splicing element (intein). Comparison with other mycobacterial...
Orphon spliced-leader sequences form part of a repetitive element in Angiostrongylus cantonensis.
Joshua, G W, Perler, F B, Wang, C C
In nematodes, the 22 nucleotide (nt) spliced leader (SL) is normally encoded by a multi-copy, tandemly reiterated SL gene and is trans-spliced from SL-RNA onto the 5' end of a subset of mRNAs. We...
Protein splicing removes intervening sequences in an archaea DNA polymerase.
Hodges, R A, Perler, F B, Noren, C J, Jack, W E
The Vent DNA polymerase gene from Thermococcus litoralis contains two in-frame insertions that must be spliced out to form the mature polymerase. Primer extension and cDNA PCR revealed no evidence of...
Xu, M Q, Comb, D G, Paulus, H, Noren, C J, Shao, Y, Perler, F B
Protein splicing involves the excision of an internal domain from a precursor protein and the ligation of the external domains so as to generate two new proteins. Study of this process has recently...
The mechanism of protein splicing and its modulation by mutation.
Protein splicing results in the expression of two mature proteins from a single gene. After synthesis of a precursor protein, an internal segment (the intein) is excised and the external domains are...
Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.
Perler, F B, Davis, E O, Dean, G E, Gimble, F S, Jack, W E, Neff, N, ...
Control of protein splicing by intein fragment reassembly.
Southworth, M W, Adam, E, Panne, D, Byer, R, Kautz, R, Perler, F B
Inteins are protein splicing elements that mediate their excision from precursor proteins and the joining of the flanking protein sequences (exteins). In this study, protein splicing was controlled...