Focher, F, Duncombe, R J, Basnak, I, Walker, R T, Coe, P L, ...
The antiviral activity of several nucleoside analogues is often limited by their rapid degradation by pyrimidine nucleoside phosphorylases. In an attempt to avoid this degradation, several modified...
Sun, M, Hamor, TA, Focher, F, Verri, A, Spadari, S, ...
The synthesis and X-ray crystal structures of a series of 5-substituted-6-aza-2'-deoxyuridines is reported. These nucleoside analogues inhibit the phosphorylation of thymidine by HSV-1 TK but have no...
Focher, F., Maga, G., Bendiscioli, A., Capobianco, M., Colonna, F., Garbesi, A., ...
L-β-Deoxythymldine (L-dT), the optical enantiomer of D-β-deoxythymidine (o-dT), and L-enantiomers of nucleoslde analogs, such as 5-iodo-2′-deoxy-L-uridine (L-ldU) and...
Late induction of human DNA ligase I after UV-C irradiation.
Montecucco, A, Savini, E, Biamonti, G, Stefanini, M, Focher, F, Ciarrocchi, G
We have studied the regulation of DNA ligase I gene expression in UV-C irradiated human primary fibroblasts. An increase of approximately 6-fold both in DNA ligase I messenger and activity levels was...
Focher, F, Maga, G, Bendiscioli, A, Capobianco, M, Colonna, F, Garbesi, A, ...
L-beta-Deoxythymidine (L-dT), the optical enantiomer of D-beta-deoxythymidine (D-dT), and L-enantiomers of nucleoside analogs, such as 5-iodo-2'-deoxy-L-uridine (L-IdU) and...
Calf thymus DNA polymerase delta independent of proliferating cell nuclear antigen (PCNA).
Focher, F, Gassmann, M, Hafkemeyer, P, Ferrari, E, Spadari, S, Hübscher, U
DNA polymerase delta from calf thymus was purified under conditions that minimized proteolysis to a specific activity of 27,000 units/mg. The four step isolation procedure included phosphocellulose,...
HP 0.35, a cephalosporin degradation product is a specific inhibitor of lentiviral RNAses H.
Hafkemeyer, P, Neftel, K, Hobi, R, Pfaltz, A, Lutz, H, Lüthi, K, ...
Penicillins, cephalosporins and other betalactam antibiotics are widely used antibacterial drugs. Recently it was found that some of them also have effects on proliferating eukaryotic cells (Neftel,...
Verri, A, Mazzarello, P, Biamonti, G, Spadari, S, Focher, F
Point mutations in the cAMP-responsive element (CRE) of the rat somatostatin gene promoter/enhancer sequence (TGACGTCA) were used as a model for assessing the effect of uracil, deriving either from...
Montecucco, A, Fontana, M, Focher, F, Lestingi, M, Spadari, S, Ciarrocchi, G
The antiviral distamycin A and its phenyl mustard derivative FCE24517 possessing antitumor activity were tested for their ability to inhibit macromolecular synthesis in three human and one murine...
DNA ligase I gene expression during differentiation and cell proliferation.
Montecucco, A, Biamonti, G, Savini, E, Focher, F, Spadari, S, Ciarrocchi, G
We have studied the regulation of mammalian DNA ligase I gene by using a cDNA probe in Northern blot experiments with RNA extracted from several cell types in different growth conditions. DNA ligase...
Focher, F, Spadari, S, Ginelli, B, Hottiger, M, Gassmann, M, Hübscher, U
We have established a novel procedure to purify calf thymus DNA polymerase delta from cytoplasmic extracts. The enzyme has typical properties of DNA polymerase delta including a 3' - greater than 5'...
Uracil-DNA glycosylases preferentially excise mispaired uracil.
Verri, A, Mazzarello, P, Spadari, S, Focher, F
We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the...
Focher, F, Verri, A, Spadari, S, Manservigi, R, Gambino, J, Wright, G E
We have purified Herpes simplex type 1 (HSV1) uracil-DNA glycosylase from the nuclei of HSV1-infected HeLa cells harvested 8 h post-infection, at which time the induction of the enzyme is a maximum....
Lack of stereospecificity of suid pseudorabies virus thymidine kinase.
Maga, G, Verri, A, Bonizzi, L, Ponti, W, Poli, G, Garbesi, A, ...
We have partially purified suid pseudorabies virus (PRV) thymidine kinase from infected thymidine kinase- mouse cells, and cytosolic swine thymidine kinase from lymphatic glands, and we have found...
Maga, G, Focher, F, Wright, G E, Capobianco, M, Garbesi, A, Bendiscioli, A, ...
It is known that the Herpes simplex virus type 1 (HSV-1)-encoded thymidine kinase (TK) co-purifies with an associated thymidylate kinase (TMPK) activity and that thymidylate (TMP) inhibits the...
Maga, G, Spadari, S, Wright, G E, Focher, F
From oocysts of the protozoan parasite Eimeria tenella, responsible for avian coccidiosis, we have partially purified and characterized a novel enzymic activity which specifically phosphorylates...
Hardt, N, Pedrali-Noy, G, Focher, F, Spadari, S
A radioautographic examination of nuclear DNA synthesis in unirradiated and u.v.-irradiated HeLa cells, in the presence and in the absence of aphidicolin, showed that aphidicolin inhibits nuclear DNA...
Verri, A, Priori, G, Spadari, S, Tondelli, L, Focher, F
Our discovery that Herpes virus thymidine kinase (TK) and cellular deoxycytidine kinase lack enantioselectivity, being able to phosphorylate both D- and L-enantiomers of the substrate, suggested the...
Trichomonas vaginalis thymidine kinase: purification, characterization and search for inhibitors.
Strosselli, S, Spadari, S, Walker, R T, Basnak, I, Focher, F
We report that a thymidine kinase (TK) activity is present in Trichomonas vaginalis and can be separated from the deoxyribonucleoside phosphotransferase. T. vaginalis TK, purified 11200-fold to...
Verri, A, Montecucco, A, Gosselin, G, Boudou, V, Imbach, J L, Spadari, S, ...
We demonstrate that l-ATP is recognized by some enzymes that are involved in the synthesis of nucleotides and nucleic acids. l-ATP, as well as its natural d-enantiomer, acts as a phosphate donor in...
Verri, A, Focher, F, Duncombe, R J, Basnak, I, Walker, R T, Coe, P L, ...
The antiviral activity of several nucleoside analogues is often limited by their rapid degradation by pyrimidine nucleoside phosphorylases. In an attempt to avoid this degradation, several modified...
Turriziani, O, Schuetz, J D, Focher, F, Scagnolari, C, Sampath, J, Adachi, M, ...
Cellular factors may contribute to the decreased efficacy of chemotherapy in HIV infection. Indeed, prolonged treatment with nucleoside analogues, such as azidothymidine (AZT), 2',3'-deoxycytidine or...
Late induction of human DNA ligase I after UV-C irradiation.
Montecucco, A, Savini, E, Biamonti, G, Stefanini, M, Focher, F, Ciarrocchi, G
We have studied the regulation of DNA ligase I gene expression in UV-C irradiated human primary fibroblasts. An increase of approximately 6-fold both in DNA ligase I messenger and activity levels was...
Focher, F, Maga, G, Bendiscioli, A, Capobianco, M, Colonna, F, Garbesi, A, ...
L-beta-Deoxythymidine (L-dT), the optical enantiomer of D-beta-deoxythymidine (D-dT), and L-enantiomers of nucleoside analogs, such as 5-iodo-2'-deoxy-L-uridine (L-IdU) and...
Calf thymus DNA polymerase delta independent of proliferating cell nuclear antigen (PCNA).
Focher, F, Gassmann, M, Hafkemeyer, P, Ferrari, E, Spadari, S, Hübscher, U
DNA polymerase delta from calf thymus was purified under conditions that minimized proteolysis to a specific activity of 27,000 units/mg. The four step isolation procedure included phosphocellulose,...
HP 0.35, a cephalosporin degradation product is a specific inhibitor of lentiviral RNAses H.
Hafkemeyer, P, Neftel, K, Hobi, R, Pfaltz, A, Lutz, H, Lüthi, K, ...
Penicillins, cephalosporins and other betalactam antibiotics are widely used antibacterial drugs. Recently it was found that some of them also have effects on proliferating eukaryotic cells (Neftel,...
Verri, A, Mazzarello, P, Biamonti, G, Spadari, S, Focher, F
Point mutations in the cAMP-responsive element (CRE) of the rat somatostatin gene promoter/enhancer sequence (TGACGTCA) were used as a model for assessing the effect of uracil, deriving either from...
Montecucco, A, Fontana, M, Focher, F, Lestingi, M, Spadari, S, Ciarrocchi, G
The antiviral distamycin A and its phenyl mustard derivative FCE24517 possessing antitumor activity were tested for their ability to inhibit macromolecular synthesis in three human and one murine...
DNA ligase I gene expression during differentiation and cell proliferation.
Montecucco, A, Biamonti, G, Savini, E, Focher, F, Spadari, S, Ciarrocchi, G
We have studied the regulation of mammalian DNA ligase I gene by using a cDNA probe in Northern blot experiments with RNA extracted from several cell types in different growth conditions. DNA ligase...
Focher, F, Spadari, S, Ginelli, B, Hottiger, M, Gassmann, M, Hübscher, U
We have established a novel procedure to purify calf thymus DNA polymerase delta from cytoplasmic extracts. The enzyme has typical properties of DNA polymerase delta including a 3' - greater than 5'...
Uracil-DNA glycosylases preferentially excise mispaired uracil.
Verri, A, Mazzarello, P, Spadari, S, Focher, F
We have investigated the substrate specificity of human, viral and bacterial uracil-DNA glycosylases employing as substrate double-stranded oligonucleotides containing in the same position of the...
Focher, F, Verri, A, Spadari, S, Manservigi, R, Gambino, J, Wright, G E
We have purified Herpes simplex type 1 (HSV1) uracil-DNA glycosylase from the nuclei of HSV1-infected HeLa cells harvested 8 h post-infection, at which time the induction of the enzyme is a maximum....
Lack of stereospecificity of suid pseudorabies virus thymidine kinase.
Maga, G, Verri, A, Bonizzi, L, Ponti, W, Poli, G, Garbesi, A, ...
We have partially purified suid pseudorabies virus (PRV) thymidine kinase from infected thymidine kinase- mouse cells, and cytosolic swine thymidine kinase from lymphatic glands, and we have found...
Maga, G, Focher, F, Wright, G E, Capobianco, M, Garbesi, A, Bendiscioli, A, ...
It is known that the Herpes simplex virus type 1 (HSV-1)-encoded thymidine kinase (TK) co-purifies with an associated thymidylate kinase (TMPK) activity and that thymidylate (TMP) inhibits the...
Maga, G, Spadari, S, Wright, G E, Focher, F
From oocysts of the protozoan parasite Eimeria tenella, responsible for avian coccidiosis, we have partially purified and characterized a novel enzymic activity which specifically phosphorylates...
Hardt, N, Pedrali-Noy, G, Focher, F, Spadari, S
A radioautographic examination of nuclear DNA synthesis in unirradiated and u.v.-irradiated HeLa cells, in the presence and in the absence of aphidicolin, showed that aphidicolin inhibits nuclear DNA...
Verri, A, Priori, G, Spadari, S, Tondelli, L, Focher, F
Our discovery that Herpes virus thymidine kinase (TK) and cellular deoxycytidine kinase lack enantioselectivity, being able to phosphorylate both D- and L-enantiomers of the substrate, suggested the...
Trichomonas vaginalis thymidine kinase: purification, characterization and search for inhibitors.
Strosselli, S, Spadari, S, Walker, R T, Basnak, I, Focher, F
We report that a thymidine kinase (TK) activity is present in Trichomonas vaginalis and can be separated from the deoxyribonucleoside phosphotransferase. T. vaginalis TK, purified 11200-fold to...
Verri, A, Montecucco, A, Gosselin, G, Boudou, V, Imbach, J L, Spadari, S, ...
We demonstrate that l-ATP is recognized by some enzymes that are involved in the synthesis of nucleotides and nucleic acids. l-ATP, as well as its natural d-enantiomer, acts as a phosphate donor in...
Verri, A, Focher, F, Duncombe, R J, Basnak, I, Walker, R T, Coe, P L, ...
The antiviral activity of several nucleoside analogues is often limited by their rapid degradation by pyrimidine nucleoside phosphorylases. In an attempt to avoid this degradation, several modified...
Turriziani, O, Schuetz, J D, Focher, F, Scagnolari, C, Sampath, J, Adachi, M, ...
Cellular factors may contribute to the decreased efficacy of chemotherapy in HIV infection. Indeed, prolonged treatment with nucleoside analogues, such as azidothymidine (AZT), 2',3'-deoxycytidine or...