F. G. Witebsky

Clarithromycin lowers plasma zidovudine levels in persons with human immunodeficiency virus infection.

Polis, M A, Piscitelli, S C, Vogel, S, Witebsky, F G, Conville, P S, Petty, B, ...

The use of antiretroviral agents and drugs for the treatment and prophylaxis of opportunistic infections has lengthened the survival of persons with AIDS. In the era of multidrug therapy, drug...

Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists' Mycobacteriology E survey: results of a 1993 questionnaire.

Woods, G L, Witebsky, F G

Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume,...

Effect of PANTA on growth of Mycobacterium kansasii in BACTEC 12B medium.

Conville, P S, Andrews, J W, Witebsky, F G

Mycobacterium kansasii isolates from two patients showed relatively slow growth in BACTEC 12B medium (12B) (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) compared with the more rapid...

Correlation of in vitro susceptibility results for amoxicillin-clavulanate and ampicillin-sulbactam tested against Escherichia coli.

O'Shaughnessy, E M, Fahle, G A, Witebsky, F G

The results of amoxicillin-clavulanate (AUG) and ampicillin-sulbactam (A/S) susceptibility testing by three different susceptibility testing methods, the MicroScan, Etest, and Kirby-Bauer methods,...

Antimicrobial susceptibilities of mycobacteria as determined by differential light scattering and correlation with results from multiple reference laboratories.

Conville, P S, Witebsky, F G, MacLowry, J D

The DAWN Model B laser light scattering instrument (Wyatt Technology Corporation, Santa Barbara, Calif.) was evaluated to assess its potential to provide rapid mycobacterial antimicrobial...

Modified toluidine blue O stain for Pneumocystis carinii: further evaluation of some technical factors.

Witebsky, F G, Andrews, J W, Gill, V J, MacLowry, J D

A modified toluidine blue O (TBO) stain for Pneumocystis carinii cysts was evaluated with regard to the influence of (i) the age and extent of use of the sulfation reagent, (ii) the source of TBO,...

Comparison of BACTEC 13A medium and Du Pont isolator for detection of mycobacteremia.

Witebsky, F G, Keiser, J F, Conville, P S, Bryan, R, Park, C H, Walker, R, ...

BACTEC 13A medium (Johnston Laboratories, Towson, Md.) was compared with Isolator (Du Pont Co., Wilmington, Del.) concentrate for sensitivity, speed, and technical ease of isolation of mycobacteria...

Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia.

Gill, V J, Park, C H, Stock, F, Gosey, L L, Witebsky, F G, Masur, H

Patients with acquired immune deficiency syndrome may develop infection with mycobacteria, particularly Mycobacterium avium-M. intracellulare (MAI). These infections can frequently be associated with...

Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia.

Gosey, L L, Howard, R M, Witebsky, F G, Ognibene, F P, Wu, T C, Gill, V J, ...

A modified toluidine blue O staining technique for Pneumocystis carinii is described. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. The stain can be employed for...

Inoculum size and lot-to-lot variation as significant variables in the tube coagulase test for Staphylococcus aureus.

Selepak, S T, Witebsky, F G

For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be...

Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories.

Witebsky, F G, Sliva, C A, Selepak, S T, Ruddel, M E, MacLowry, J D, Johnson, E E, ...

Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva),...

beta-Lactamase detection in nine staphylococcal species.

Selepak, S T, Witebsky, F G

Detection of beta-lactamase by four different techniques (two acidometric, one chromogenic cephalosporin, and one iodometric) was evaluated with 79 isolates of nine different species of...

Microbiology subsystem of a total, dedicated laboratory computer system.

Lawrie, D J, Elin, R J, Gill, V J, Lewis, T L, MacLowry, J D, Witebsky, F G

The computer system used by the Microbiology Service of the Clinical Pathology Department, Clinical Center, National Institutes of Health is discussed. This microbiology subsystem is a part of a...

Evaluation of serum gentamicin assay procedures for a clinical microbiology laboratory.

Lantz, C H, Lawrie, D J, Witebsky, F G, MacLowry, J D

Four methods for the measurement of serum gentamicin concentration were evaluated with respect to cost-effectiveness, accuracy, and precision. Gentamicin concentration was determined in 112 clinical...

Evaluation of five gentamicin assay procedures for clinical microbiology laboratories.

Selepak, S T, Witebsky, F G, Robertson, E A, MacLowry, J D

Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was...

Broth dilution minimum inhibitory concentrations: rationale for use of selected antimicrobial concentrations.

Witebsky, F G, Maclowry, J D, French, S S

A microdilution susceptibility testing procedure utilizing selected, clinically relevant concentrations of a large number of antimicrobial agents is described. A qualitative code designed to...

Clarithromycin lowers plasma zidovudine levels in persons with human immunodeficiency virus infection.

Polis, M A, Piscitelli, S C, Vogel, S, Witebsky, F G, Conville, P S, Petty, B, ...

The use of antiretroviral agents and drugs for the treatment and prophylaxis of opportunistic infections has lengthened the survival of persons with AIDS. In the era of multidrug therapy, drug...

Mycobacterial testing in clinical laboratories that participate in the College of American Pathologists' Mycobacteriology E survey: results of a 1993 questionnaire.

Woods, G L, Witebsky, F G

Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume,...

Effect of PANTA on growth of Mycobacterium kansasii in BACTEC 12B medium.

Conville, P S, Andrews, J W, Witebsky, F G

Mycobacterium kansasii isolates from two patients showed relatively slow growth in BACTEC 12B medium (12B) (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.) compared with the more rapid...

Correlation of in vitro susceptibility results for amoxicillin-clavulanate and ampicillin-sulbactam tested against Escherichia coli.

O'Shaughnessy, E M, Fahle, G A, Witebsky, F G

The results of amoxicillin-clavulanate (AUG) and ampicillin-sulbactam (A/S) susceptibility testing by three different susceptibility testing methods, the MicroScan, Etest, and Kirby-Bauer methods,...

Antimicrobial susceptibilities of mycobacteria as determined by differential light scattering and correlation with results from multiple reference laboratories.

Conville, P S, Witebsky, F G, MacLowry, J D

The DAWN Model B laser light scattering instrument (Wyatt Technology Corporation, Santa Barbara, Calif.) was evaluated to assess its potential to provide rapid mycobacterial antimicrobial...

Modified toluidine blue O stain for Pneumocystis carinii: further evaluation of some technical factors.

Witebsky, F G, Andrews, J W, Gill, V J, MacLowry, J D

A modified toluidine blue O (TBO) stain for Pneumocystis carinii cysts was evaluated with regard to the influence of (i) the age and extent of use of the sulfation reagent, (ii) the source of TBO,...

Comparison of BACTEC 13A medium and Du Pont isolator for detection of mycobacteremia.

Witebsky, F G, Keiser, J F, Conville, P S, Bryan, R, Park, C H, Walker, R, ...

BACTEC 13A medium (Johnston Laboratories, Towson, Md.) was compared with Isolator (Du Pont Co., Wilmington, Del.) concentrate for sensitivity, speed, and technical ease of isolation of mycobacteria...

Use of lysis-centrifugation (isolator) and radiometric (BACTEC) blood culture systems for the detection of mycobacteremia.

Gill, V J, Park, C H, Stock, F, Gosey, L L, Witebsky, F G, Masur, H

Patients with acquired immune deficiency syndrome may develop infection with mycobacteria, particularly Mycobacterium avium-M. intracellulare (MAI). These infections can frequently be associated with...

Advantages of a modified toluidine blue O stain and bronchoalveolar lavage for the diagnosis of Pneumocystis carinii pneumonia.

Gosey, L L, Howard, R M, Witebsky, F G, Ognibene, F P, Wu, T C, Gill, V J, ...

A modified toluidine blue O staining technique for Pneumocystis carinii is described. An easily prepared sulfation reagent made with sulfuric and acetic acids was used. The stain can be employed for...

Inoculum size and lot-to-lot variation as significant variables in the tube coagulase test for Staphylococcus aureus.

Selepak, S T, Witebsky, F G

For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be...

Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories.

Witebsky, F G, Sliva, C A, Selepak, S T, Ruddel, M E, MacLowry, J D, Johnson, E E, ...

Accuracy, precision, and clinical laboratory utility of the TDX (Abbott Laboratories), Auto-ICS (Beckman Instruments, Inc.), COBAS-Bio (Roche Analytical Instruments, Inc.) with reagent kits (Syva),...

beta-Lactamase detection in nine staphylococcal species.

Selepak, S T, Witebsky, F G

Detection of beta-lactamase by four different techniques (two acidometric, one chromogenic cephalosporin, and one iodometric) was evaluated with 79 isolates of nine different species of...

Microbiology subsystem of a total, dedicated laboratory computer system.

Lawrie, D J, Elin, R J, Gill, V J, Lewis, T L, MacLowry, J D, Witebsky, F G

The computer system used by the Microbiology Service of the Clinical Pathology Department, Clinical Center, National Institutes of Health is discussed. This microbiology subsystem is a part of a...

Evaluation of serum gentamicin assay procedures for a clinical microbiology laboratory.

Lantz, C H, Lawrie, D J, Witebsky, F G, MacLowry, J D

Four methods for the measurement of serum gentamicin concentration were evaluated with respect to cost-effectiveness, accuracy, and precision. Gentamicin concentration was determined in 112 clinical...

Evaluation of five gentamicin assay procedures for clinical microbiology laboratories.

Selepak, S T, Witebsky, F G, Robertson, E A, MacLowry, J D

Five gentamicin assay procedures (a bioassay, an enzyme immunoassay, a latex agglutination inhibition test, a fluorescence immunoassay, and a radioimmunoassay) were evaluated to determine which was...

Broth dilution minimum inhibitory concentrations: rationale for use of selected antimicrobial concentrations.

Witebsky, F G, Maclowry, J D, French, S S

A microdilution susceptibility testing procedure utilizing selected, clinically relevant concentrations of a large number of antimicrobial agents is described. A qualitative code designed to...