Glycogen synthase 2 is a novel target gene of peroxisome proliferator-activated receptors (2007)
Mandard, S.J., Stienstra, R., Escher, P., Tan, N.S., Kim, I., Gonzalez, F.J., ...
Glycogen synthase 2 (Gys-2) is the ratelimiting enzyme in the storage of glycogen in liver and adipose tissue, yet little is known about regulation of Gys-2 transcription. The peroxisome...
The peroxisome proliferator-activated receptor alpha regulates amino acid metabolism (2001)
Kersten, S., Mandard, S., Escher, P., Gonzalez, F.J., Tafuri, S., Desvergne, B., ...
The peroxisome proliferator-activated receptor is a ligand-activated transcription factor that plays an important role in the regulation of lipid homeostasis. PPAR mediates the effects of fibrates,...
Michalik, L., Desvergne, B., Tan, N. S., Basu-Modak, S., Escher, P., Rieusset, J., ...
We show here that the alpha, beta, and gamma isotypes of peroxisome proliferator-activated receptor (PPAR) are expressed in the mouse epidermis during fetal development and that they disappear...
Akiyama, T E, Nicol, C J, Fievet, C, Staels, B, Ward, J M, Auwerx, J, ...
Considerable controversy exists in determining the role of peroxisome proliferator-activated receptor-alpha (PPARalpha) in obesity. Two purebred congenic strains of PPARalpha-null mice were developed...
Broadband GaAs MMIC downconverter with very low intermodulation for TV applications (1999)
De La Fuente, M.L., Pascual, J. P., Gonzalez, F. J., Artal, E.
A novel combination of a packaged GaAs MMIC with a microstrip-coupled slot high performance 180° hybrid constitutes a downconverter, which provides the very low intermodulation level required by...
Dihydropyrimidine dehydrogenase pharmacogenetics in Caucasian subjects (1998)
Ridge, S.A., Sludden, J., Brown, O., Robertson, L., Wei, X., Sapone, A., ...
Nomenclature for human DPYD alleles (1998)
McLeod, H.L., Johnson, M.R., Wei, X., Sapone, A., Diasio, R.B., ...
Peters, J M, Hennuyer, N, Staels, B, Fruchart, J C, Fievet, C, Gonzalez, F J, ...
The peroxisome proliferator-activated receptor-alpha (PPARalpha) controls gene expression in response to a diverse class of compounds collectively referred to as peroxisome proliferators. Whereas...
Metabolic differences in colon mucosal cells (1993)
McKinnon, R. A., Burgess, W. M., Gonzalez, F. J., McManus, M. E.
The colonic expression of cytochromes P450 from the CYP1A, CYP3A and CYP4B subfamilies has been characterized in rabbit and human tissues using RNA blotting, immunoblotting, immunohistochemistry and...
Metabolic differences in colon mucosal cells (1993)
McKinnon, R. A., Burgess, W. M., Gonzalez, F. J., McManus, M. E.
The colonic expression of cytochromes P450 from the CYP1A, CYP3A and CYP4B subfamilies has been characterized in rabbit and human tissues using RNA blotting, immunoblotting, immunohistochemistry and...
Neonatal lethality associated with respiratory distress in mice lacking cytochrome P450 1A2.
Pineau, T, Fernandez-Salguero, P, Lee, S S, McPhail, T, Ward, J M, Gonzalez, F J
Cytochrome P450 1A2 (CYP1A2) is a constitutively expressed hepatic enzyme that is highly conserved among mammals. This protein is primarily involved in oxidative metabolism of xenobiotics and is...
Aoyama, T, Yamano, S, Guzelian, P S, Gelboin, H V, Gonzalez, F J
Twelve forms of human hepatic cytochrome P450 were expressed in hepatoma cells by means of recombinant vaccinia viruses. The expressed P450s were analyzed for their abilities to activate the potent...
Aoyama, T, Nagata, K, Yamazoe, Y, Kato, R, Matsunaga, E, Gelboin, H V, ...
A cDNA containing the full coding region of human cytochrome b5 was inserted into a vaccinia virus cDNA expression vector. Infection of human thymidine kinase-minus (TK-) 143 cells in culture with...
Lee, S S, Pineau, T, Drago, J, Lee, E J, Owens, J W, Kroetz, D L, ...
To gain insight into the function of peroxisome proliferator-activated receptor (PPAR) isoforms in rodents, we disrupted the ligand-binding domain of the alpha isoform of mouse PPAR (mPPAR alpha) by...
Lee, Y H, Williams, S C, Baer, M, Sterneck, E, Gonzalez, F J, Johnson, P F
The rat CYP2D5 P-450 gene is activated in the liver during postnatal development. We previously showed that liver-specific transcription of the CYP2D5 gene is dictated by a proximal promoter element,...
Disruption of the c/ebp alpha gene in adult mouse liver.
Lee, Y H, Sauer, B, Johnson, P F, Gonzalez, F J
The liver-enriched transcription factor C/EBP alpha has been implicated in the regulation of numerous liver-specific genes. It was previously reported that mice carrying a homozygous null mutation at...
Skoda, R C, Gonzalez, F J, Demierre, A, Meyer, U A
The "debrisoquine polymorphism" is a clinically important genetic defect of drug metabolism affecting 5-10% of individuals in Caucasian populations. It is inherited as an autosomal recessive trait. A...
Petersen, D D, Gonzalez, F J, Rapic, V, Kozak, C A, Lee, J Y, Jones, J E, ...
The NAD(P)H:menadione oxidoreductase gene (Nmo-1) codes for a quinone reductase (also called DT diaphorase; EC 1.6.99.2) believed to play a central role in protection against oxidative stress. We...
cDNA and deduced amino acid sequences of human P450 IIA3 (CYP2A3).
Yamano, S, Nagata, K, Yamazoe, Y, Kato, R, Gelboin, H V, Gonzalez, F J
Mouse cytochrome P3-450: complete cDNA and amino acid sequence.
Kimura, S, Gonzalez, F J, Nebert, D W
A full-length cDNA clone (1,894 nucleotides) of mouse cytochrome P3-450 was isolated with the Okayama-Berg vector and sequenced. An open reading frame spanned positions 61 to 1602. The first 25, and...
Jaiswal, A K, Gonzalez, F J, Nebert, D W
The human P1-450 gene (6,311 base pairs), as well as the 5' (1,604 bases) and 3' (113 bases) flanking regions, have been completely sequenced. Four highly homologous boxes (61, 82, 56 and 97 base...
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is known to interact with a cytosolic receptor and, in turn, activate transcription of the mouse P1(450) gene. Various lengths of DNA upstream of the...
Sequence of the 5' end of the developmentally regulated rat P450 PB1 (P450IIC6) gene.
Umeno, M, Morris, S, Matsunaga, E, Gelboin, H V, Gonzalez, F J
Hildebrand, C E, Gonzalez, F J, McBride, O W, Nebert, D W
The human 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 full-length cDNA has been recently isolated and sequenced [Jaiswal, A.K., Gonzalez, F.J. and Nebert, D.W. (1985) Science, in...
Lee, Y H, Yano, M, Liu, S Y, Matsunaga, E, Johnson, P F, Gonzalez, F J
The rat CYP2D5 gene encodes a cytochrome P450 and is expressed in liver cells. Its expression commences a few days after birth, and maximal mRNA levels are achieved when animals reach puberty....
Transcriptional control of the rat hepatic CYP2E1 gene.
The rat hepatic CYP2E1 gene becomes transcriptionally activated within 1 day after birth. This activation can be mimicked by using the 5' end of the gene in a cell-free nuclear extract prepared from...
Mizuno, K, Gonzalez, F J, Kimura, S
A cDNA clone encoding a thyroid-specific enhancer-binding protein (T/EBP) was isolated from a rat thyroid-derived FRTL-5 cell lambda gt 11 expression library, using a double-stranded oligonucleotide...
Kikkawa, F, Gonzalez, F J, Kimura, S
A 6.3-kbp segment of DNA, upstream of the human thyroid peroxidase gene, and various deletions thereof were linked to a promoterless bacterial chloramphenicol acetyltransferase reporter gene. These...
Yano, M, Falvey, E, Gonzalez, F J
The CYP2C6 gene becomes maximally transcriptionally activated in livers of postpubertal rats. We examined the role of upstream DNA and liver-specific transcription factors in regulation of this...
Kimura, S, Gonzalez, F J, Nebert, D W
Expression of the P(1)450 and P(3)450 genes was examined in liver and five extrahepatic tissues of mice after they were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or...
Gonzalez, F J, Song, B J, Hardwick, J P
A cytochrome P-450 cDNA clone, designated pP450PCN2, homologous to the previously characterized pregnenolone 16 alpha-carbonitrile (PCN)-induced P-450 cDNA (pP450PCN1; F. J. Gonzalez, D. W. Nebert,...
Molecular basis of the human dihydropyrimidine dehydrogenase deficiency and 5-fluorouracil toxicity.
Wei, X, McLeod, H L, McMurrough, J, Gonzalez, F J, Fernandez-Salguero, P
Dihydropyrimidine dehydrogenase (DPD) deficiency constitutes an inborn error in pyrimidine metabolism associated with thymine-uraciluria in pediatric patients and an increased risk of toxicity in...
Djouadi, F, Weinheimer, C J, Saffitz, J E, Pitchford, C, Bastin, J, Gonzalez, F J, ...
The peroxisome proliferator-activated receptor alpha (PPARalpha) is a nuclear receptor implicated in the control of cellular lipid utilization. To test the hypothesis that PPARalpha is activated as a...
Long term freeze storage of Campylobacter pyloridis.
Westblom, T U, Barthel, J S, Havey, A D, Gonzalez, F J, Tarka, E F, Everett, E D
Human cytochromes P450: evolution and cDNA-directed expression.
As the first step in the process of carcinogenesis, most chemical carcinogens require metabolic activation by cytochromes P450 for conversion to highly reactive electrophiles that bind covalently to...
Kimura, S, Umeno, M, Skoda, R C, Meyer, U A, Gonzalez, F J
The debrisoquine-4-hydroxylase polymorphism is a genetic variation in oxidative drug metabolism characterized by two phenotypes, the extensive metabolizer (EM) and poor metabolizer (PM). Of the...
Hanioka, N, Kimura, S, Meyer, U A, Gonzalez, F J
The debrisoquine polymorphism is a common genetic defect that results in deficient oxidation of debrisoquine and numerous other drugs. These compounds are metabolized by a form of cytochrome P450,...
Neonatal lethality associated with respiratory distress in mice lacking cytochrome P450 1A2.
Pineau, T, Fernandez-Salguero, P, Lee, S S, McPhail, T, Ward, J M, Gonzalez, F J
Cytochrome P450 1A2 (CYP1A2) is a constitutively expressed hepatic enzyme that is highly conserved among mammals. This protein is primarily involved in oxidative metabolism of xenobiotics and is...
Aoyama, T, Yamano, S, Guzelian, P S, Gelboin, H V, Gonzalez, F J
Twelve forms of human hepatic cytochrome P450 were expressed in hepatoma cells by means of recombinant vaccinia viruses. The expressed P450s were analyzed for their abilities to activate the potent...
Aoyama, T, Nagata, K, Yamazoe, Y, Kato, R, Matsunaga, E, Gelboin, H V, ...
A cDNA containing the full coding region of human cytochrome b5 was inserted into a vaccinia virus cDNA expression vector. Infection of human thymidine kinase-minus (TK-) 143 cells in culture with...
Lee, S S, Pineau, T, Drago, J, Lee, E J, Owens, J W, Kroetz, D L, ...
To gain insight into the function of peroxisome proliferator-activated receptor (PPAR) isoforms in rodents, we disrupted the ligand-binding domain of the alpha isoform of mouse PPAR (mPPAR alpha) by...
Lee, Y H, Williams, S C, Baer, M, Sterneck, E, Gonzalez, F J, Johnson, P F
The rat CYP2D5 P-450 gene is activated in the liver during postnatal development. We previously showed that liver-specific transcription of the CYP2D5 gene is dictated by a proximal promoter element,...
Disruption of the c/ebp alpha gene in adult mouse liver.
Lee, Y H, Sauer, B, Johnson, P F, Gonzalez, F J
The liver-enriched transcription factor C/EBP alpha has been implicated in the regulation of numerous liver-specific genes. It was previously reported that mice carrying a homozygous null mutation at...
Skoda, R C, Gonzalez, F J, Demierre, A, Meyer, U A
The "debrisoquine polymorphism" is a clinically important genetic defect of drug metabolism affecting 5-10% of individuals in Caucasian populations. It is inherited as an autosomal recessive trait. A...
Petersen, D D, Gonzalez, F J, Rapic, V, Kozak, C A, Lee, J Y, Jones, J E, ...
The NAD(P)H:menadione oxidoreductase gene (Nmo-1) codes for a quinone reductase (also called DT diaphorase; EC 1.6.99.2) believed to play a central role in protection against oxidative stress. We...
cDNA and deduced amino acid sequences of human P450 IIA3 (CYP2A3).
Yamano, S, Nagata, K, Yamazoe, Y, Kato, R, Gelboin, H V, Gonzalez, F J
Mouse cytochrome P3-450: complete cDNA and amino acid sequence.
Kimura, S, Gonzalez, F J, Nebert, D W
A full-length cDNA clone (1,894 nucleotides) of mouse cytochrome P3-450 was isolated with the Okayama-Berg vector and sequenced. An open reading frame spanned positions 61 to 1602. The first 25, and...
Jaiswal, A K, Gonzalez, F J, Nebert, D W
The human P1-450 gene (6,311 base pairs), as well as the 5' (1,604 bases) and 3' (113 bases) flanking regions, have been completely sequenced. Four highly homologous boxes (61, 82, 56 and 97 base...
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is known to interact with a cytosolic receptor and, in turn, activate transcription of the mouse P1(450) gene. Various lengths of DNA upstream of the...
Sequence of the 5' end of the developmentally regulated rat P450 PB1 (P450IIC6) gene.
Umeno, M, Morris, S, Matsunaga, E, Gelboin, H V, Gonzalez, F J
Hildebrand, C E, Gonzalez, F J, McBride, O W, Nebert, D W
The human 2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible cytochrome P1-450 full-length cDNA has been recently isolated and sequenced [Jaiswal, A.K., Gonzalez, F.J. and Nebert, D.W. (1985) Science, in...
Lee, Y H, Yano, M, Liu, S Y, Matsunaga, E, Johnson, P F, Gonzalez, F J
The rat CYP2D5 gene encodes a cytochrome P450 and is expressed in liver cells. Its expression commences a few days after birth, and maximal mRNA levels are achieved when animals reach puberty....
Transcriptional control of the rat hepatic CYP2E1 gene.
The rat hepatic CYP2E1 gene becomes transcriptionally activated within 1 day after birth. This activation can be mimicked by using the 5' end of the gene in a cell-free nuclear extract prepared from...
Mizuno, K, Gonzalez, F J, Kimura, S
A cDNA clone encoding a thyroid-specific enhancer-binding protein (T/EBP) was isolated from a rat thyroid-derived FRTL-5 cell lambda gt 11 expression library, using a double-stranded oligonucleotide...
Kikkawa, F, Gonzalez, F J, Kimura, S
A 6.3-kbp segment of DNA, upstream of the human thyroid peroxidase gene, and various deletions thereof were linked to a promoterless bacterial chloramphenicol acetyltransferase reporter gene. These...
Yano, M, Falvey, E, Gonzalez, F J
The CYP2C6 gene becomes maximally transcriptionally activated in livers of postpubertal rats. We examined the role of upstream DNA and liver-specific transcription factors in regulation of this...
Kimura, S, Gonzalez, F J, Nebert, D W
Expression of the P(1)450 and P(3)450 genes was examined in liver and five extrahepatic tissues of mice after they were treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or...
Gonzalez, F J, Song, B J, Hardwick, J P
A cytochrome P-450 cDNA clone, designated pP450PCN2, homologous to the previously characterized pregnenolone 16 alpha-carbonitrile (PCN)-induced P-450 cDNA (pP450PCN1; F. J. Gonzalez, D. W. Nebert,...
Molecular basis of the human dihydropyrimidine dehydrogenase deficiency and 5-fluorouracil toxicity.
Wei, X, McLeod, H L, McMurrough, J, Gonzalez, F J, Fernandez-Salguero, P
Dihydropyrimidine dehydrogenase (DPD) deficiency constitutes an inborn error in pyrimidine metabolism associated with thymine-uraciluria in pediatric patients and an increased risk of toxicity in...
Djouadi, F, Weinheimer, C J, Saffitz, J E, Pitchford, C, Bastin, J, Gonzalez, F J, ...
The peroxisome proliferator-activated receptor alpha (PPARalpha) is a nuclear receptor implicated in the control of cellular lipid utilization. To test the hypothesis that PPARalpha is activated as a...
Long term freeze storage of Campylobacter pyloridis.
Westblom, T U, Barthel, J S, Havey, A D, Gonzalez, F J, Tarka, E F, Everett, E D
Human cytochromes P450: evolution and cDNA-directed expression.
As the first step in the process of carcinogenesis, most chemical carcinogens require metabolic activation by cytochromes P450 for conversion to highly reactive electrophiles that bind covalently to...
Kimura, S, Umeno, M, Skoda, R C, Meyer, U A, Gonzalez, F J
The debrisoquine-4-hydroxylase polymorphism is a genetic variation in oxidative drug metabolism characterized by two phenotypes, the extensive metabolizer (EM) and poor metabolizer (PM). Of the...
Hanioka, N, Kimura, S, Meyer, U A, Gonzalez, F J
The debrisoquine polymorphism is a common genetic defect that results in deficient oxidation of debrisoquine and numerous other drugs. These compounds are metabolized by a form of cytochrome P450,...
Brooks, B A, McBride, O W, Dolphin, C T, Farrall, M, Scambler, P J, Gonzalez, F J, ...
CYP3, the gene which encodes the hepatic cytochrome P450pcn1, the isozyme responsible for the metabolic oxidation of the calcium channel-blocking drug nifedipine, has recently been mapped to human...
Characterisation of CYP3A gene subfamily expression in human gastrointestinal tissues.
McKinnon, R A, Burgess, W M, Hall, P M, Roberts-Thomson, S J, Gonzalez, F J, McManus, M E
The human CYP3A subfamily is of interest due to its multiplicity, activity toward known carcinogens, and extrahepatic expression. In situ hybridisation analysis of formalin fixed, routinely processed...
Birkett, D J, Rees, D, Andersson, T, Gonzalez, F J, Miners, J O, Veronese, M E
1. The activation of proguanil to cycloguanil by human liver microsomes was studied to define the cytochrome P450 (CYP) isoforms involved in this reaction. 2. Apparent Km values for proguanil ranged...
Adachi, M, Kurotani, R, Morimura, K, Shah, Y, Sanford, M, Madison, B B, ...
Mutations at codon 974 of the DPYD gene are a rare event.
Ridge, S. A., Brown, O., McMurrough, J., Fernandez-Salguero, P., Evans, W. E., Gonzalez, F. J., ...
A mutation at codon 974 of the dihydropyrimidine dehydrogenase (DPD) gene was previously described in a cancer patient with undetectable DPD enzyme activity who experienced severe toxicity when...
Dihydropyrimidine dehydrogenase pharmacogenetics in patients with colorectal cancer.
Ridge, S. A., Sludden, J., Wei, X., Sapone, A., Brown, O., Hardy, S., ...
Individuals with a deficiency in the enzyme dihydropyrimidine dehydrogenase (DPD) may experience severe life-threatening toxicity when treated with 5-fluorouracil (5-FU). As routine measurement of...
Modulation of erlotinib pharmacokinetics in mice by a novel cytochrome P450 3A4 inhibitor, BAS 100
Smith, N F, Baker, S D, Gonzalez, F J, Harris, J W, Figg, W D, Sparreboom, A
Administration of BAS 100, a novel mechanism-based CYP3A4 inhibitor isolated from grapefruit juice, resulted in a 2.1-fold increase in erlotinib exposure following oral administration to wild-type...
Glycogen synthase 2 is a novel target gene of peroxisome proliferator-activated receptors
Mandard, S., Stienstra, R., Escher, P., Tan, N. S., Kim, I., Gonzalez, F. J., ...
Glycogen synthase 2 (Gys-2) is the ratelimiting enzyme in the storage of glycogen in liver and adipose tissue, yet little is known about regulation of Gys-2 transcription. The peroxisome...