F. M. Klis

Mass spectrometry-based proteomics of fungal wall glycoproteins (2008)

Yin, Q.Y., Koster, C.G. De, Klis, F.M.

The manifold functions of fungal wall glycoproteins include maintenance of cell wall integrity, homotypic and heterotypic adhesion, biofilm formation, acquisition of iron and sterols, protein...

Hypoxic conditions and iron restriction affect the cell-wall proteome of Candida albicans grown under vagina-simulative conditions (2008)

Sosinska, G.J., Teixeira De Mattos, M.J., Dekker, H.L., Koster, C.G. De, Hellingwerf, K.J., ...

Proteins that are covalently linked to the skeletal polysaccharides of the cell wall of Candida albicans play a major role in the colonization of the vaginal mucosal surface, which may result in...

The cell wall of the human pathogen Candida glabrata: Differential incorporation of novel adhesin-like wall proteins (2008)

Kraneveld, E.A., Yin, Q.Y., Dekker, H.L., Gross, U., Crielaard, W., ...

The cell wall of the human pathogen Candida glabrata governs initial host-pathogen interactions that underlie the establishment of fungal infections. With the aim of identifying species-specific...

A novel screening method for cell wall mutants in Aspergillus niger identifies UDP-galactopyranose mutase as an important protein in fungal cell wall biosynthesis (2008)

Damveld, R.A., Franken, A., Arentshorst, M., Punt, P.J., Klis, F.M., ...

To identify cell wall biosynthetic genes in filamentous fungi and thus potential targets for the discovery of new antifungals, we developed a novel screening method for cell wall mutants. It is based...

The conserved PA14 domain of cell wall-associated fungal adhesins governs their glycan-binding specificity (2008)

Klis, F.M.

Yeast cell wall-associated, lectin-like adhesins form large families that mediate flocculation and host cell recognition. The glycan specificity of individual adhesins is largely unknown. Zupancic et...

Identification, characterization, and phenotypic analysis of covalently linked cell wall proteins. (2007)

Klis, F.M., Brul, S.

Cell wall biology of Candida. (2007)

Brandt, B.W., Klis, F.M.

Identification, characterization, and phenotypic analysis of covalently linked cell wall proteins. (2007)

Klis, F.M., Brul, S.

Cellular processes and pathways that protect Saccharomyces cerevisiae cells against the plasma membrane-perturbing compound chitosan. (2007)

Zakrzewska, A.M., Boorsma, A., Delneri, D., Brul, S., Oliver, S.G., Klis, F.M.

Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae. (2007)

Yin, Q., Jong, L. De, Klis, F.M., Koster, C.G. De

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. (2007)

Pel, H.J., Winde, J.H. De, Archer, D.B., Dyer, P.S., Hofmann, G., Klis, F.M., ...

The CRH family coding for cell wall glycosylphosphatidylinositol. proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. (2007)

Pardini, G., Coste, A.T., Karababa, M., Klis, F.M., Koster, C.G. De, ...

Extraction of cell surface-associated proteins from living yeast cells. (2007)

Klis, F.M., Jong, M. De, Brul, S.

Mass spectrometric identification of covalently bound cell wall proteins from the fission yeast Schizosaccharomyces pombe. (2007)

Yin, Q., Weig, M., Sosinska, G.J., Klis, F.M., Koster, C.G. De

Cell wall biology of Candida. (2007)

Brandt, B.W., Klis, F.M.

A molecular and genomic view of the fungal cell wall (2007)

Klis, F.M.

Extraction of cell surface-associated proteins from living yeast cells. (2007)

Klis, F.M., Jong, M. De, Brul, S.

Extraction of cell surface-associated proteins from living yeast cells. (2007)

Klis, F.M., Jong, M. De, Brul, S.

A molecular and genomic view of the fungal cell wall (2007)

Klis, F.M.

Identification, characterization, and phenotypic analysis of covalently linked cell wall proteins. (2007)

Klis, F.M., Brul, S.

Cellular processes and pathways that protect Saccharomyces cerevisiae cells against the plasma membrane-perturbing compound chitosan. (2007)

Zakrzewska, A.M., Boorsma, A., Delneri, D., Brul, S., Oliver, S.G., Klis, F.M.

Mass spectrometric quantitation of covalently bound cell wall proteins in Saccharomyces cerevisiae. (2007)

Yin, Q., Jong, L. De, Klis, F.M., Koster, C.G. De

Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. (2007)

Pel, H.J., Winde, J.H. De, Archer, D.B., Dyer, P.S., Hofmann, G., Klis, F.M., ...

The CRH family coding for cell wall glycosylphosphatidylinositol. proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. (2007)

Pardini, G., Coste, A.T., Karababa, M., Klis, F.M., Koster, C.G. De, ...

Mass spectrometric identification of covalently bound cell wall proteins from the fission yeast Schizosaccharomyces pombe. (2007)

Yin, Q., Weig, M., Sosinska, G.J., Klis, F.M., Koster, C.G. De

Cell wall biology of Candida. (2007)

Brandt, B.W., Klis, F.M.

Identification of cell wall-associated proteins from Phytophthora ramorum (2006)

Meijer, H.J.G., Yin, Q.Y., Klis, F.M., Govers, F., ...

Mechanism of action of the endo-(1→3)-α-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum (2006)

Grun, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

Mechanism of action of the endo-(1--3)-alpha-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum. (2006)

Grün, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

Mechanism of action of the endo-(1-->3)-alpha-glucanase MutAp from the mycoparasitic fungus Trichoderma harzianum (2006)

Grün, C.H., Dekker, N., Nieuwland, A.A., Klis, F.M., Kamerling, J.P., Vliegenthart, J.F.G., ...

Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. (2006)

Ram, A.F.J., Klis, F.M.

The CRH family coding for cell wall glycosylphosphatidylinositol proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. (2006)

Pardini, G., Coste, A.T., Karababa, M., Klis, F.M., Koster, C.G. De, ...

Identification of cell wall-associated proteins from Phytophthora ramorum. (2006)

Meijer, H.J., Vondervoort, P.J. Van, Yin, Q., Koster, C.G. De, Klis, F.M., Govers, F., ...

Cell wall construction in Saccharomyces cerevisiae. (2006)

Klis, F.M., Boorsma, A.

Flocculation, adhesion and biofilm formation in yeasts. (2006)

Verstrepen, K.J., Klis, F.M.

Trends and highlights in Candida research. (2006)

Klis, F.M.

Role of cell-cycle regulated expression in the localized incorporation of cell wall proteins in yeast. (2006)

Smits, G.J., Schenkman, L.R., Brul, S., Pringle, J.R., Klis, F.M.

Glycosylphosphatidylinositol-anchored proteases of Candida albicans target proteins necessary for both cellular processes and host-pathogen interactions. (2006)

Albrecht, A., Felk, A, Pichova, I, Naglik, J.R., Schaller, M., ...

Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. (2006)

Ram, A.F., Klis, F.M.

Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. (2006)

Ram, A.F.J., Klis, F.M.

The CRH family coding for cell wall glycosylphosphatidylinositol proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. (2006)

Pardini, G., Coste, A.T., Karababa, M., Klis, F.M., Koster, C.G. De, ...

Identification of cell wall-associated proteins from Phytophthora ramorum. (2006)

Meijer, H.J., Vondervoort, P.J. Van, Yin, Q., Koster, C.G. De, Klis, F.M., Govers, F., ...

Cell wall construction in Saccharomyces cerevisiae. (2006)

Klis, F.M., Boorsma, A.

Flocculation, adhesion and biofilm formation in yeasts. (2006)

Verstrepen, K.J., Klis, F.M.

Trends and highlights in Candida research. (2006)

Klis, F.M.

Role of cell-cycle regulated expression in the localized incorporation of cell wall proteins in yeast. (2006)

Smits, G.J., Schenkman, L.R., Brul, S., Pringle, J.R., Klis, F.M.

Glycosylphosphatidylinositol-anchored proteases of Candida albicans target proteins necessary for both cellular processes and host-pathogen interactions. (2006)

Albrecht, A., Felk, A, Pichova, I, Naglik, J.R., Schaller, M., ...

Activation of the protein kinase C1 pathway upon continuous heat stress in S.cerevisiae is triggered by an increase in osmolarity due to trehalose accumulation. (2005)

Mensonides, F.I.C., Brul, S., Klis, F.M., Hellingwerf, K.J., Teixeira De Mattos, M.J.

This paper reports on physiological and molecular responses of Saccharomyces cerevisiae to heat stress conditions. We observed that within a very narrow range of culture temperatures, a shift from...

CandidaDB: a genome database for Candida albicans pathogenomics. (2005)

D'Enfert, C., Goyard, S., Rodriguez-Arnaveilhe, S., Frangeul, L., Jones, L., Tekaia, F., ...

Expression of agsA, one of five 1,3-alpha-D-glucan synthease-encoding genes in Aspergillus niger, is induced in response to cell wall stress. (2005)

Damveld, R.A., Vankuyk, P.A., Arentshorst, M., Klis, F.M., Ram, A.F.

1,3-alpha-D-Glucan is an important component of the cell wall of filamentous fungi. We have identified a family of five 1,3-alpha-D-glucan synthase-encoding genes in Aspergillus niger. The agsA gene...

Granulocytes govern the transcriptional response, morphology and proliferation of Candida albicans in human blood. (2005)

Fradin, C., Maccallum, D., Schaller, M., Klis, F.M., Odds, F.C., ...

Survival in blood and escape from blood vessels into tissues are essential steps for the yeast Candida albicans to cause systemic infections. To elucidate the influence of blood components on fungal...

Features and functions of covalently linked proteins in fungal cell walls. (2005)

Ram, A.F., Klis, F.M.

The cell walls of many ascomycetous yeasts consist of an internal network of stress-bearing polysaccharides, which serve as a scaffold for a dense external layer of glycoproteins. GPI-modified...

T-profiler: scoring the activity of predefined groups of genes using gene expression data. (2005)

Boorsma, A., Foat, B.C., Vis, D.J., Klis, F.M., Bussemaker, H.J.

One of the key challenges in the analysis of gene expression data is how to relate the expression level of individual genes to the underlying transcriptional programs and cellular state. Here we...

The Aspergillus niger MADS-box transcription factor RlmA is required for cell wall reinforcement in response to cell wall stress. (2005)

Damveld, R.A., Arentshorst, M., Franken, A., Vankuyk, P.A., Klis, F.M., ...

In Aspergillus niger, the genes coding for glutamine:fructose-6-phosphate amidotransferase (gfaA) and alpha-1,3-glucan synthase (agsA) are induced in response to cell wall stress. In silico analysis...

The structure of cell wall alpha-glucan from fission yeast. (2005)

Grun, C.H., Hochstenbach, F., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

Morphology and structural integrity of fungal cells depend on cell wall polysaccharides. The chemical structure and biosynthesis of two types of these polysaccharides, chitin and...

Transcriptional response of Saccharomyces cerevisiae to the plasma membrane-perturbing compound chitosan. (2005)

Zakrzewska, A.M., Boorsma, A., Brul, S., Hellingwerf, K.J., Klis, F.M.

Chitosan is a plasma membrane-perturbing compound consisting of linear chains of ?-1,4-linked glucosamine residues, which at acidic pHs become positively charged. It is extensively used as an...

The structure of cell wall alpha-glucan from fission yeast (2005)

Grün, C.H., Hochstenbach, F.M.H., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

The structure of cell wall α-glucan from fission yeast (2005)

Grun, C.H., Hochstenbach, F., Humbel, B.M., Verkleij, A.J., Sietsma, J.H., Klis, F.M., ...

Comprehensive proteomic analysis of Saccharomyces cerevisiae cell walls: identification of protein glycosylphosphatidylinositol remnants or mild alkali-sensitive linkages. (2005)

Yin, Q., Dekker, H.L., Jong, L. De, Klis, F.M., Koster, C.G. De

Characterisation of CwpA, a putative glycosylphosphatidylinositol-anchored cell wall mannoprotein in the filamentous fungus Aspergillus niger. (2005)

Damveld, R.A., Arentshorst, M., Vankuyk, P.A., Klis, F.M., Ram, A.F.

The transcriptional response of yeast to the plasma membrane perturbing compound chitosan. (2005)

Zakrzewska, A.M., Boorsma, A., Brul, S., Hellingwerf, K.J., Klis, F.M.

CandidaDB: a genome database for Candida albicans pathogenomics (2005)

D'Enfert, C., Goyard, S., Rodriguez-Arnaveilhe, S., Frangeul, L., Jones, L., Tekaia, F., ...

CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology...

Systematic identification in silico of covalently bound cell wall proteins and analysis of protein-polysaccharide linkages of the human pathogen Candida glabrata. (2004)

Weig, M., Jansch, L., Gross, U., Koster, C.G. De, Klis, F.M.

Proteomic analysis of Candida albicans cell walls reveals covalently bound carbohydrate-active enzymes and adhesins. (2004)

Boer, A.D. De, Cunningham, J., Dekker, H.L., Jong, L. De, Hellingwerf, K.J., ...

Molecular Organisation and Biogenesis of the cell wall. (2004)

Klis, F.M., Brul, S., Hellingwerf, K.J.

An in vitro assay for (1-->6)-beta-D-glucan synthesis in Saccharomyces cerevisiae. (2004)

Vink, E., Rodriguez-Suarez, R.J., Gerard-Vincent, M., Ribas, J.C., Nobel, J.G. De, Ende, H. Van Den, ...

(1 --> 6)-beta-D-glucan is a key cell wall component of Saccharomyces cerevisiae and Candida albicans. Many genes are known to affect the levels or structure of this glucan, but their roles and a...

Characterization of the transcriptional response to cell wall stress in Saccharomyces cerevisiae. (2004)

Boorsma, A., Nobel, H. De, Riet, B. Ter, Bargmann, B., Brul, S., Hellingwerf, K.J., ...

The cell wall perturbants Calcofluor white and Zymolyase activate the Pkc1-Rho1-controlled Slt2p MAP kinase pathway in Saccharomyces cerevisiae. A downstream transcription factor of this pathway,...

Food preservation and the development of microbial resistance; past present and future. (2003)

Brul, S., Klis, F.M., Knorr, D., Abee, T., Notermans, S.

Organization and construction of the yeast cell wall (2003)

Nobel, H. De, Klis, F.M.

Yeast and food spoilage (2003)

Brul, S., Vossen, J. Van Der, Boorsma, A., Klis, F.M.

Yeast stress response to food preservation systems. (2003)

Brul, S., Klis, F.M., Coote, P., Hellingwerf, K.J.

Conference report. The second int.conference on molecular mechanisms of Fungal cell wall biogenesis. Salamanga, Spain. (2003)

Klis, F.M.

Genome-wide identification of fungal GPI proteins. (2003)

Hellingwerf, K.J., Klis, F.M.

The protein kinase Kic1 affects 1,6-beta-glucan levels in the cell wall of Saccharomyces cerevisiae (2002)

Vink, E., Vossen, J.H., Ram, A.F.J., Ende, H. Van Den, Brekelmans, S.S.C., Nobel, J.G. De, ...

Genomics of survivors of food preservation processes for precision processing (2002)

Brul, S., Montijn, R., Schuren, F., Oomes, S., Klis, F.M., Coote, P., ...

Physiological actions of preservative agents: prospective of use of modern microbiological techniques in assessing microbial behaviour in food preservation. (2002)

Brul, S., Coote, P., Mensonides, F.I.C., Hellingwerf, K.J., Klis, F.M.

GP17 affects cell-wall protein anchorage in Saccharomyces cerevisiae and Candida albicans (2002)

Richard, M., Courtin, O., Poulain, D., Klis, F.M., Gaillardin, C.

Dynamics of cell wall structure in Saccharomyces cerevisiae (2002)

Klis, F.M., Mol, P.C., Hellingwerf, K.J., Brul, S.

A new strategy for the inhibition of the spoilage yeast Saccharomyces cerevisiae and Zygosaccharomyces bailii based on the combination of a membrane-active peptide with an oligosaccharide that leads to an impaired GPI-dependent yeast wall protein layer. (2001)

Bom, I.J., Nobel, J.G. De, Klis, F.M., Brul, S.

Proteomics and genomics of sorbic acid stress in yeast (2001)

Nobel, J.G. De, Lewrie, L., Brul, S., Klis, F.M., Alloush, H., Coote, P.

Unravelling the mode-of-action of preservation processes through functional genomics on survivors (2001)

Brul, S., Klis, F.M., Coote, P.

Molecular organization of the cell wall of Candida albicans (2001)

Klis, F.M., Hellingwerf, K.J.

Differential regulation of cell wall biogenesis during growth and development in yeast (2001)

Smits, G.J., Ende, H. Van Den, Klis, F.M.

Sacchromyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway (2001)

Martin-Yken, H., Dagkessamanskaia, A., Ram, A.F.J., Klis, F.M., Francois, J.

Low external pH induces HOG1-dependent changes in the organization of the Saccharomyces cerevisiae cell wall (2001)

Kapteyn, J.C., Riet, B. Ter, Vink, E., Blad, S., Nobel, H. De, Ende, H. Van Den, ...

Characterization of agglutinin-like sequence genes from non-albicans Candida and Phylogenetic analysis of the ALS family (2001)

Hoyer, L.L., Fundyga, R., Hecht, J.E., Kapteyn, J.C., Klis, F.M., Arnold, J.

A genomic approach for the idenfitication and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae (2001)

Ruiz, C., Vazquez De Aldana, C.R., Duenas, E., Cid, V.J., Del Rey, F., ...

Parallel and comparative analysis of the proteome and transcriptome of sorbic acid-stressed Saccharomyces cerevisiae (2001)

De Nobel, H., Lawrie, L., Brul, S., Klis, F.M., Davis, M., Alloush, H., ...

Cell wall perturbation in yeast results in dual phosphorylation of the Slt2/Mpk1 MAP kinase and in an Slt2-mediated increase in FKS2-lacZ expression, glucanase resistance and thermotolerance (2000)

Nobel, J.G. De, Ruiz, C., Martin, H., Morris, W., Brul, S., Molina, M., ...

Cell wall maintenance in fungi (2000)

Nobel, J.G. De, Ende, H. Van Den, Klis, F.M.

Functional analysis of genes involved in cell wall biosynthesis of Saccharomyces (1999)

Andel, A., Brekelmans, S.S.C., Klis, F.M.

Cell wall dynamics in yeast (1999)

Smits, G.J., Kapteijn, J.C., Ende, H. Van Den, Klis, F.M.

The contribution of cell wall proteins to the organization of the yeast cell wall (1999)

Kapteijn, J.C., Ende, H. Van Den, Klis, F.M.

The contribution of the 0-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta-1,6-glucan-deficient mutants (1999)

Kapteijn, J.C., Egmond, P. Van, Sievi, E., Ende, H. Van Den, Makarow, M., Klis, F.M.

Characterization of protein-glucan complexes in the cell wall of Saccharomyces cerevisiae and Candida albicans (1999)

Klis, F.M., Kapteijn, J.C., Smits, G.J., Ende, H. Van Den

Dynamics of the molecular architecture of the cell wall of Saccharomyces cerevisiae (1999)

Klis, F.M., Kapteijn, J.C.

Genes involved in cell wall construction. Identification of genes involved in maturation and assembly of cell wall components (1999)

Klis, F.M., Andel, A., Brekelmans, S.S.C.

The cell wall of Fusarium oxysporum (1999)

Schoffelmeer, E.A.M., Klis, F.M., Sietsma, J.H., Cornelissen, B.J.C.

Review Mechanistic and mathematical inactivation studies of food spoilage fungi (1999)

Brul, S., Klis, F.M.

The Saccharomyces cerevisiae CWH8 gene is required for full levels of dolichol-linked oligosaccharides in the endoplasmic reticulum and for efficient N-glycosylation (1999)

Rieger, M., Heesen, S. Te, Ram, A.F.J., Ende, H. Van Den, Aebi, M., ...

Identification of the essential EPE1 gene involved in retention of secreted proteins on the cell surface of Saccharomyces cerevisiae cells (1999)

Alexieva, K.I., Klis, F.M., Wedler, H., Wambutt, R., Venkov, P.

Localization of synthesis of beta1,6-glucan in Saccharomyces cerevisiae (1999)

Montijn, R.C., Vink, E., Muller, W.H., Verkleij, A.J., Ende, H. Van Den, Henrissat, B., ...

Localized deposition of GPI-cell wall proteins in Saccharomyces cerevisiae (1999)

Smits, G.J., Sietsma, H., Ende, H. Van Den, Klis, F.M.

The cell wall of fusarium oxysporum (1999)

Schoffelmeer, E.A.M., Klis, F.M., Sietsma, J.H., Cornelissen, B.J.C.

Loss of the plasma membrane-bound protein gas1p in saccharomyces cerevisiae results in the release of beta1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity. (1998)

Ram, A.F.J., Kapteyn, J.C., Montijn, R.C., Caro, L.H.P., Douwes, J.E., Baginsky, W., ...

A new tool for studying the molecular architecture of the fungal cell wall: one-step purification of recombinant Trichoderma beta-(1-6)-glucanase expressed in Pichia pastoris (1998)

Bom, IJ., Dielbandhoesing, S.K., Harvey, K.N., Klis, F.M., Brul, S.

Changes in cell wall architecture in Saccharomyces cerevisiae mutants with reduced levels of cell wall b1,6-glucan (1998)

Kapteijn, J.C., Vink, E., Moukadiri, I., Zueco, J., Klis, F.M.

Identification of a cell wall repair mechanism in Saccharomyces cerevisiae (1998)

Klis, F.M., Kapteijn, J.C., Ram, A.F.J.

Functional analysis of genes involved in cell wall biosynthesis of Saccharomyces cerevisiae (1998)

Andel, A., Brekelmans, S.S.C., Klis, F.M.

Specific cell wall proteins confer resistance to nisin upon yeast cells (1998)

Dielbandhoesing, S., Zhang, H., Caro, L.H.P., Klis, F.M., Verrips, C.T., ...

Identification and characterization of cell wall related (CWR) genes (1998)

Klis, F.M., Molina, M., Ruiz, C., Rey, F. Del, Vasquez, C.

The cell wall proteins Cwp1 and Cwp2 are directed to specific regions of the wall (1998)

Smits, G.J., Ram, A.F.J., Caro, L.H.P., Klis, F.M.

Identification of an alpha-glucan synthase essential for morphogenesis in fission yeast Schizosaccharomyces pombe (1998)

Hochstenbach, F.M.H., Klis, F.M., Ende, H. Van Den, Klausner, R.D.

Control of cell wall construction: identification of multicopy suppressors of the protein kinase mutant cwh30/kic1 (1998)

Vink, E., Vossen, J.H., Brekelmans, S.S.C., Ende, H. Van Den, Klis, F.M.

Evidence that beta- 1,6-glucan synthesis takes place at the cell surface (1998)

Montijn, R.C., Vink, E., Muller, W., Verklei, A., Volland, C., Seron, K., ...

Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast (1998)

Hochstenbach, F.M.H., Klis, F.M., Ende, H. Van Den, Donselaar, E., Peters, P.J., Klausner, R.D.

Specific cell wall proteins confer resistance to nisin upon yeast cells (1998)

Dielbandhoesing, S., Zhang, H., Caro, L.H.P., Klis, F.M., Verrips, C.T., ...

Green fluorescent protein-cell wall fusion proteins are covalently incorporated into the cell wall of Saccharomyces cerevisiae (1998)

Ram, A.F.J., Ende, H. Van Den, Klis, F.M.

Posttranslational modifications of secretory proteins (1998)

Klis, F.M., Ram, A.F.J., Montijn, R.C., Kapteijn, J.C., Caro, L.H.P., Vossen, J.H., ...

Transcription of multiple cell wall protein-encoding genes in Saccharomyces cerevisiae is differentially regulated during the cell cycle (1998)

Caro, L.H.P., Smits, G.J., Egmond, P. Van, Chapman, J.W., Klis, F.M.

Stress responses in fungi in relation to the molecular architecture of the cell wall (1998)

Nobel, J.G. De, Kapteijn, J.C., Ram, A.F.J., Klis, F.M.

Loss of the plasma membrane-bound protein Gas1p in Saccharomyces cerevisiae results in the release of beta 1,3-glucan into the medium and induces a compensation mechanism to ensure cell wall integrity (1998)

Ram, A.F.J., Kapteijn, J.C., Montijn, R.C., Caro, L.H.P., Douwes, J.E., Baginsky, W., ...

The antifungal action of 1,10-o-phenanthroline and EDTA is mediated through zinc chelation and involves cell wall construction (1997)

Brul, S., Stratford, M., Dielbandhoesing, S., Steels, H., Klis, F.M., ...

The incorporation of mannoproteins in the cell wall of S. cerevisiae and filamentous Ascomycetes (1997)

Brul, S., King, A., Chapman, J.W., Klis, F.M., Verrips, C.T.

Characterization of cell wall-related genes in yeast (1997)

Klis, F.M., Caro, L.H.P., Molina, M., Nombela, C., Castro, C., Cos, T. De

Identification and characterization of the major building block of the cell wall in yeast and other fungi (1997)

Klis, F.M., Caro, L.H.P., Vossen, J.H., Ende, H. Van Den

Architecture of the yeast cell wall, beta(1-6)glucan interconnects mannoprotein, beta(1-3)-glucan, and chitin (1997)

Kollar, R., Reinhold, B.B., Petrakova, E., Yeh, H.J.C., Ashwell, G., Drgonova, J., ...

In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae (1997)

Caro, L.H.P., Tettelin, H., Vossen, J.H., Ram, A.F.J., Ende, H. Van Den, Klis, F.M.

Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae (1997)

Lussier, M., White, A.M., Sheraton, J., DiPaolo, T., Treadwell, J., Southard, S.B., ...

Altered extent of cross-linking of beta 1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta 1,3-glucan content (1997)

Kapteijn, J.C., Ram, A.F.J., Groos, E.M., Kollar, R., Montijn, R.C., Ende, H. Van Den, ...

Loss of the plasma membrane-bound protein GAS1P results in the release of beta-1,3-glucan into the medium and activates a cell wall repair mechanism (1997)

Ram, A.F.J., Kapteijn, J.C., Montijn, R.C., Caro, L.H.P., Douwes, J.E., Baginsky, W., ...

Inefficient N-glycosylation in S. cerevisiae CW8 cells results from lower levels of dolichol-linked precursor oligosaccharides in the endoplasmatic reticulum (1997)

Heesen, S. Te, Ende, H. Van Den, Aebi, M., Klis, F.M.

The expression of multiple cell wall proteins of Saccharomyces cerevisiae is cell cycle regulated (1997)

Smits, G.J., Caro, L.H.P., Ram, A.F.J., Egmond, P. Van, Chapman, J.W., Klis, F.M.

Identification and characterization of a major building block in the cell wall (1997)

Klis, F.M., Caro, L.H.P., Vossen, J.H., Ende, H. Van Den

Incorporation of proteins in the cell wall of fungi (1997)

Klis, F.M., Caro, L.H.P., Kapteijn, J.C., Montijn, R.C.

Beta-glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitides (1997)

Montijn, R.C., Wolven, P. Van, Hoog, G.S. De, Klis, F.M.

Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins (1997)

Biesebeke, R. Te, Chapman, J.W., Toschka, H.Y., Klis, F.M., Verrips, C.T.

Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked ß-1,3-/ß-1,6-glucan heteropolymer. (1996)

Kapteyn, J.C., Montijn, R.C., Vink, E., Llobell, A., Douwes, J.E., ...

Glucosylation of fungal cell wall proteins as a potential target for novel antifungal agents (1996)

Schoffelmeer, E.A.M., Kapteijn, J.C., Montijn, R.C., Cornelissen, B.J.C., Klis, F.M.

The importance of Zn2+ ions for fungal growth (1996)

Brul, S., Stratford, M., Dielbandhoesing, S., Oomes, S., Chapman, J.W., ...

The incorporation mechanism of fungal cell wall proteins (1996)

Klis, F.M., Kapteijn, J.C., Ram, A.F.J., Montijn, R.C.

The retention mechanism of cell wall proteins in Saccharomyces cerevisiae. Wall-bound Cwp2p is beta-1,6-glucosylated (1996)

Schagen, F.S. Van, Mooren, A.T.A., Chapman, J.W., Klis, F.M., Verrips, C.T.

The beta-1,6-glucan containing side-chain of cell wall proteins of Saccharomyces cerevisiae is bound to the glycan core of the GPI moiety (1996)

Biesebeke, R. Te, Chapman, J.W., Klis, F.M., Verrips, C.T.

Kre6p and Skn1p are involved in the elongation of N-chains. A new role for Kre6p and Skn1p in the biogenesis of cell wall proteins (1996)

Montijn, R.C., Vink, E., Roemer, T., Bussey, H., Lipke, P.N., Klis, F.M.

Cell-cycle regulated expression of cell wall protein encoding genes in Saccharomyces cerevisiae (1996)

Caro, L.H.P., Egmond, P. Van, Chapman, J.W., Klis, F.M.

CWH30 encondes the serine/threonine kinase Kic1/Nrk1 and is involved in beta 1-6 glucan synthesis (1996)

Vossen, J.H., Brekelmans, S.S.C., Boerma, M., Ram, A.F.J., Bussey, H., Klis, F.M.

The role of CWH8 and CWH36 in cell wall assembly (1996)

Rieger, M., Ram, A.F.J., Kapteijn, J.C., Caro, L.H.P., Klis, F.M.

Pleiotropic effects due to the loss of the major GPI anchored plasma membrane protein (Gas1p) in S. cerevisiae. A possible role for Gas1p in cell wall construction (1996)

Ram, A.F.J., Kapteijn, J.C., Montijn, R.C., Douwes, J.E., Caro, L.H.P., Mazur, P., ...

Hyphal cell wall proteins of the tomato pathogen Fusarium oxysporum (1996)

Schoffelmeer, E.A.M., Klis, F.M., Cornelissen, B.J.C.

Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked beta-1,3-/beta-1,6-glucan heteropolymer (1996)

Kapteijn, J.C., Montijn, R.C., Vink, E., Cruz, J. De La, Llobell, A., Douwes, J.E., ...

CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta-1,6-glucan assembly (1996)

Jiang, B., Sheraton, J., Ram, A.F.J., Dijkgraaf, G.J.P., Klis, F.M., Bussey, H.

Yeast expressing hepatitis B virus antigen determinants on its surface: implications for a possible oral vaccine (1996)

Schreuder, M.P., Deen, C., Boersma, W.J.A., Pouwels, P.H., Klis, F.M.

Immobilizing proteins on the surface of yeast cells (1996)

Schreuder, M.P., Mooren, A.T.A., Toschka, H.Y., Verrips, C.T., Klis, F.M.

Glucosylation of fungal cell wall proteins as a potential target for novel antifungal agents (1996)

Schoffelmeer, E.A.M., Kapteijn, J.C., Montijn, R.C., Cornelissen, B.J.C., Klis, F.M.

Composition and structure of the cell wall of Fusarium oxysporum (1995)

Schoffelmeer, E.A.M., Klis, F.M., Elgersma, D.M., Cornelissen, B.J.C.

Glucosylation of fungal cell wall proteins as a potential target for novel antifungal agents (1995)

Schoffelmeer, E.A.M., Kapteijn, J.C., Montijn, R.C., Cornelissen, B.J.C., Klis, F.M.

The anchoring mechanism of cell wall proteins in Saccharomyces cerevisiae (1995)

Klis, F.M.

The isolation and characterization of mutants in Saccharomyces cerevisiae defective in the synthesis of beta-1,3-glucan (1995)

Ram, A.F.J., Klis, F.M.

Defective GPI anchor biosynthesis in the mutant cwh6/spt14 causes aberrant biogenesis of cell wall proteins (1995)

Vossen, J.H., Ram, A.F.J., Klis, F.M.

Characterisation of a cell wall mannoprotein of Saccharomyces cerevisiae (1995)

Schagen, F.A. Van, Caro, L.H.P., Chapman, J.W., Klis, F.M., Verrips, C.T.

Genes involved in the synthesis of beta-(1-3)-glucan. Identification of a GAS1 homolog (1995)

Ram, A.F.J., Brekelmans, S.S.C., Klis, F.M.

Intracellular synthesis of beta(1-6)-glucan in yeast in a KRE6 and SKN1-dependent fashion (1995)

Montijn, R.C., Roemer, T., Bussey, H., Klis, F.M.

O-glycosylation in plants (1995)

Klis, F.M.

Identification of SPT14/CWH6 as the yeast homologue of hPIG-A, a gene involved in the biosynthesis of GPI anchors (1995)

Vossen, J.H., Ram, A.F.J., Klis, F.M.

Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae (1995)

Caro, L.H.P., Chapman, J.W., Klis, F.M., Verrips, C.T.

Identification of two cell cycle regulated genes affecting the beta1,3-glucan content of cell walls in Saccharomyces cerevisiae (1995)

Ram, A.F.J., Brekelmans, S.S.C., Klis, F.M.

Glycosyl phosphatidylinositol-dependent cross-linking of alpha-agglutinin and beta1,6-glucan in the Saccharomyces cerevisiae cell wall (1995)

Montijn, R.C., Brown, J.L., Klis, F.M., Kurjan, J., Bussey, H., ...

Covalent association of beta-1,3-glucan with beta-1,6-glucosylated mannoproteins in cell walls of Candida albicans (1995)

Kapteijn, J.C., Montijn, R.C., Dijkgraaf, G.J.P., Ende, H. Van Den, Klis, F.M.

Glucosylation of cell wall proteins in regenerating spheroplasts of Candida albicans (1995)

Kapteijn, J.C., Dijkgraaf, G.J.P., Montijn, R.C., Klis, F.M.

Regulation of cell wall beta-glucan assembly: PTC1 negatively affects PBS2 action in a pathway that includes modulation of EXG1 transcription (1995)

Jiang, B., Ram, A.F.J., Sheraton, J., Klis, F.M., Bussey, H.

Domain conservation in several volvocalean cell wall proteins (1994)

Woessner, J.P., Molendijk, A.J., Egmond, P. Van, Klis, F.M., Goodenough, U.W., Haring, M.A.

Protein secretion in yeast (1994)

Klis, F.M.

Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae (1994)

Caro, L.H.P., Montijn, R.C., Klis, F.M.

Identification of SPT14 as a gene involved in early GPI-anchor synthesis (1994)

Vossen, J.H., Ram, A.F.J., Klis, F.M.

Identification of a family of mannoproteins in the cell wall of Saccharomyces cerevisiae (1994)

Caro, L.H.P., Chapman, J.W., Klis, F.M., Verrips, C.T.

Isolation and characterization of calcofluor white hypersensitive cell wall mutants (1994)

Ram, A.F.J., Brekelmans, S.S.C., Klis, F.M.

Identification of glucomannoproteins in yeast and hyphal cell walls of Candida albicans (1994)

Kapteijn, J.C., Montijn, R.C., Dijkgraaf, G.J.P., Klis, F.M.

Intracellular attachment of beta(1-6)-glucan to cell wall proteins of yeast in a KRE6- and SKN1-dependent fashion (1994)

Montijn, R.C., Roemer, T., Bussey, H., Klis, F.M.

Identification of beta-1,6-glucosylated cell wall proteins in yeast and hyphal forms of Candida albicans (1994)

Kapteijn, J.C., Montijn, R.C., Dijkgraaf, G.J.P., Klis, F.M.

The linkage of (1-3)-beta-glucan to chitin during cell wall assembly in Saccharomyces cerevisiae (1994)

Hartlant, R.P., Vermeulen, C.A., Klis, F.M., Sietsma, J.H., Wessels, J.G.H.

Review: Cell wall assembly in yeast. (1994)

Klis, F.M.

A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white (1994)

Ram, A.F.J., Wolters, A., Hoopen, R. Ten, Klis, F.M.

Glucomannoproteins in the cell wall of Saccharomyces cerevisiae contain a novel type of carbohydrate side chain (1994)

Montijn, R.C., Rinsum, J. Van, Schagen, F.A. Van, Klis, F.M.

Glucosylation of chimeric proteins in the cell wall of Saccharomyces cerevisiae (1994)

Caro, L.H.P., Montijn, R.C., Klis, F.M.

Characterization of the cell-cycle in synchronous cultures of Chlamydomonas-Eugametos in relation to gametogenesis (1992)

Molendijk, A.J., Vanegmond, P., Haring, M.A., Klis, F.M., Vandenende, H.

Specific Cell Wall Proteins Confer Resistance to Nisin upon Yeast Cells

Dielbandhoesing, S. K., Zhang, H., Caro, L. H. P., Van Der Vaart, J. M., Klis, F. M., Verrips, C. T., ...

The cell wall of a yeast cell forms a barrier for various proteinaceous and nonproteinaceous molecules. Nisin, a small polypeptide and a well-known preservative active against gram-positive bacteria,...

Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins.

Van Der Vaart, J M, Te Biesebeke, R, Chapman, J W, Toschka, H Y, Klis, F M, Verrips, C T

The carboxyl-terminal regions of five cell wall proteins (Cwp1p, Cwp2p, Ag alpha 1p, Tip1p, and Flo1p) and three potential cell wall proteins (Sed1p, YCR89w, and Tir1p) all proved capable of...

Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae.

Van Der Vaart, J M, Caro, L H, Chapman, J W, Klis, F M, Verrips, C T

Three glucanase-extractable cell wall proteins from Saccharomyces cerevisiae were purified, and their N-terminal amino acid sequences were determined. With this information, we were able to assign...

Covalent association of beta-1,3-glucan with beta-1,6-glucosylated mannoproteins in cell walls of Candida albicans.

Kapteyn, J C, Montijn, R C, Dijkgraaf, G J, Klis, F M

Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a...

CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.

Jiang, B, Sheraton, J, Ram, A F, Dijkgraaf, G J, Klis, F M, Bussey, H

CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in...

Restrictive glycosylphosphatidylinositol anchor synthesis in cwh6/gpi3 yeast cells causes aberrant biogenesis of cell wall proteins.

Vossen, J H, Müller, W H, Lipke, P N, Klis, F M

We previously reported that the defects in the Saccharomyces cerevisiae cwh6 Calcofluor white-hypersensitive cell wall mutant are caused by a mutation in SPT14/GPI3, a gene involved in...

Altered extent of cross-linking of beta1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta1,3-glucan content.

Kapteyn, J C, Ram, A F, Groos, E M, Kollar, R, Montijn, R C, ...

The yeast cell wall contains beta1,3-glucanase-extractable and beta1,3-glucanase-resistant mannoproteins. The beta1,3-glucanase-extractable proteins are retained in the cell wall by attachment to a...

CandidaDB: a genome database for Candida albicans pathogenomics

D'Enfert, C., Goyard, S., Rodriguez-Arnaveilhe, S., Frangeul, L., Jones, L., Tekaia, F., ...

CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology...

Large Scale Identification of Genes Involved in Cell Surface Biosynthesis and Architecture in Saccharomyces Cerevisiae

Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...

The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...

Characterization of agglutinin-like sequence genes from non-albicans Candida and phylogenetic analysis of the ALS family.

Hoyer, L L, Fundyga, R, Hecht, J E, Kapteyn, J C, Klis, F M, Arnold, J

The ALS (agglutinin-like sequence) gene family of Candida albicans encodes cell-surface glycoproteins implicated in adhesion of the organism to host surfaces. Southern blot analysis with ALS-specific...

Specific Cell Wall Proteins Confer Resistance to Nisin upon Yeast Cells

Dielbandhoesing, S. K., Zhang, H., Caro, L. H. P., Van Der Vaart, J. M., Klis, F. M., Verrips, C. T., ...

The cell wall of a yeast cell forms a barrier for various proteinaceous and nonproteinaceous molecules. Nisin, a small polypeptide and a well-known preservative active against gram-positive bacteria,...

Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins.

Van Der Vaart, J M, Te Biesebeke, R, Chapman, J W, Toschka, H Y, Klis, F M, Verrips, C T

The carboxyl-terminal regions of five cell wall proteins (Cwp1p, Cwp2p, Ag alpha 1p, Tip1p, and Flo1p) and three potential cell wall proteins (Sed1p, YCR89w, and Tir1p) all proved capable of...

Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae.

Van Der Vaart, J M, Caro, L H, Chapman, J W, Klis, F M, Verrips, C T

Three glucanase-extractable cell wall proteins from Saccharomyces cerevisiae were purified, and their N-terminal amino acid sequences were determined. With this information, we were able to assign...

Covalent association of beta-1,3-glucan with beta-1,6-glucosylated mannoproteins in cell walls of Candida albicans.

Kapteyn, J C, Montijn, R C, Dijkgraaf, G J, Klis, F M

Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a...

CWH41 encodes a novel endoplasmic reticulum membrane N-glycoprotein involved in beta 1,6-glucan assembly.

Jiang, B, Sheraton, J, Ram, A F, Dijkgraaf, G J, Klis, F M, Bussey, H

CWH41 encodes a novel type II integral membrane N-glycoprotein located in the endoplasmic reticulum. Disruption of the CWH41 gene leads to a K1 killer toxin-resistant phenotype and a 50% reduction in...

Restrictive glycosylphosphatidylinositol anchor synthesis in cwh6/gpi3 yeast cells causes aberrant biogenesis of cell wall proteins.

Vossen, J H, Müller, W H, Lipke, P N, Klis, F M

We previously reported that the defects in the Saccharomyces cerevisiae cwh6 Calcofluor white-hypersensitive cell wall mutant are caused by a mutation in SPT14/GPI3, a gene involved in...

Altered extent of cross-linking of beta1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta1,3-glucan content.

Kapteyn, J C, Ram, A F, Groos, E M, Kollar, R, Montijn, R C, ...

The yeast cell wall contains beta1,3-glucanase-extractable and beta1,3-glucanase-resistant mannoproteins. The beta1,3-glucanase-extractable proteins are retained in the cell wall by attachment to a...

CandidaDB: a genome database for Candida albicans pathogenomics

D'Enfert, C., Goyard, S., Rodriguez-Arnaveilhe, S., Frangeul, L., Jones, L., Tekaia, F., ...

CandidaDB is a database dedicated to the genome of the most prevalent systemic fungal pathogen of humans, Candida albicans. CandidaDB is based on an annotation of the Stanford Genome Technology...

Large Scale Identification of Genes Involved in Cell Surface Biosynthesis and Architecture in Saccharomyces Cerevisiae

Lussier, M., White, A. M., Sheraton, J., Di-Paolo, T., Treadwell, J., Southard, S. B., ...

The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes...

Characterization of agglutinin-like sequence genes from non-albicans Candida and phylogenetic analysis of the ALS family.

Hoyer, L L, Fundyga, R, Hecht, J E, Kapteyn, J C, Klis, F M, Arnold, J

The ALS (agglutinin-like sequence) gene family of Candida albicans encodes cell-surface glycoproteins implicated in adhesion of the organism to host surfaces. Southern blot analysis with ALS-specific...