Koning, C.J.M., Stobberingh, E.E., Mulder, L., Rombouts, F.M., Stockbrügger, R.W.
BACKGROUND: One of the side effects of antimicrobial therapy is a disturbance of the intestinal microbiota potentially resulting in antibiotic-associated diarrhea (AAD). In this placebo-controlled...
Dung, N.T.P., Rombouts, F.M., Nout, M.J.R.
In the Mekong Delta region of South-Vietnam, wine from purple glutinous rice is particularly interesting because of its sherry-like taste and flavour and its attractive brown-red colour. It is...
Thanh, N.V., Rombouts, F.M., Nout, M.J.R.
The viability and various physiological characteristics of individual sporangiospores of Rhizopus oligosporus in tempe starter cultures that had been stored for 8, 10, 16 and 30 months were examined...
Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.
Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhoea in children and piglets. Infection of ETEC results in fluid secretion and electrolyte losses in the small intestine. In this...
Timmerman, H.M., Niers, L.E.M., Ridwan, B.U., Koning, C.J.M., Mulder, L., Akkermans, L.M.A., ...
Background & aims Although the potential for probiotics is investigated in an increasing variety of diseases, there is little or no consensus regarding the desired probiotic properties for a...
Nutritional support for the infant's immune system (2007)
Niers, L., Stasse-Wolthuis, M., Rombouts, F.M., Rijkers, G.T.
Newborn babies possess a functional but immature immune system as a defense against a world teeming with microorganisms. Breast milk contains a number of biological, active compounds that support the...
Nout, M.J.R., Rombouts, F.M., Houben, J.H.
We compared acidified and lactic acid fermented silage approaches for the preservation of blue-jack mackerel. Silages acidified with formic and propionic acids had stable pH (3.8) and low (19 mg/g N)...
Bucio Galindo, A., Hartemink, R., Schrama, J.W., Verreth, J.A.J., Rombouts, F.M.
Lactobacilli are Gram-positive and catalase negative rods commonly found in lactic acid fermented foods and in the gastrointestinal tract of mammals and birds. Few studies have described lactobacilli...
Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.
Infectious diarrhoea is a major problem in both children and piglets. Infection of enterotoxigenic Escherichia coli (ETEC) results in fluid secretion and electrolyte losses in the small intestine. In...
Timmerman, H.M., Veldman, A., Rombouts, F.M., Beynen, A.C.
For application in broiler production, we developed a multispecies (MSPB) and a chicken-specific (CSPB) probiotic preparation in fluid form. The MSPB contained different probiotic species of human...
Functionality of selected strains of moulds and yeasts from Vietnamese rice wine starters (2006)
Dung, N.T.P., Rombouts, F.M., Nout, M.J.R.
The role of starch-degrading mycelial fungi, and the alcohol production and ethanol tolerance of the yeasts isolated from selected Vietnamese traditional rice wine starters were examined, and optimum...
Kiers, J.L., Hoogendoorn, A., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.
In the small intestinal segment perfusion model the effect of osmolality on net fluid absorption in enterotoxigenic Escherichia coli (ETEC)-infected and non-infected small intestinal segments of...
Prevalence of potentially pathogenic Bacillus cereus in food commodities in The Netherlands (2006)
Wijnands, L.M., Dufrenne, J.B., Rombouts, F.M.
Randomly selected food commodities, categorized in product groups, were investigated for the presence and number of Bacillus cereus bacteria. If positive, and when possible, five separate colonies...
Bucio Galindo, A., Hartemink, R., Schrama, J.W., Verreth, J.A.J., Rombouts, F.M.
A good probiotic strain should be able to survive the conditions of handling and storage to be delivered in high concentration to the host. That is especially important when stressful conditions are...
Differentiation inside multicelled macroconidia of Fusarium culmorum during early germination (2005)
Chitarra, G.S., Breeuwer, P., Rombouts, F.M., Abee, T., Dijksterhuis, J.
Multicelled conidia are formed by many fungal species, but germination of these spores is scarcely studied. Here, the germination and the effects of antimicrobials on multicompartment macroconidia of...
As a first step in the development of defined fungal starter granules for controlled winemaking from purple glutinous rice, the interaction of moulds and yeasts isolated from Vietnamese rice wine...
Health and growth of veal calves fed milk replacers with or without probiotics (2005)
Timmerman, H.M., Mulder, L., Everts, H., Klaassen, G., ...
Four experiments with 1-wk-old veal calves were conducted to assess the influence of probiotics on growth and health indicators. In experiments 1 and 2, the liquid probiotic supplements were...
Position paper on probiotics (2005)
Tijsseling, I.A., Pekelharing, P.R., Rombouts, F.M.
Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Probiotics, used to supplement normal daily nutrition, are therefore an important...
Gut flora in health and disease : potential role of probiotics (2005)
Heselmans, M., Reid, G., Akkermans, L.M.A., Savelkoul, H.F.J., Timmerman, H., Rombouts, F.M.
In a young evolving science, there are always more questions than answers. That is also the situation in the emerging field of Probiotics, and this was made very clear at the International Probiotics...
Chitarra, G.S., Abee, T., Rombouts, F.M., Dijksterhuis, J.
1-Octen-3-ol is a volatile germination self-inhibitor produced by Penicillium paneum that blocks the germination process. The size of conidia treated with 1-octen-3-ol was similar to that of freshly...
Screening of lactobacilli from fish intestines to select a probiotic for warm freshwater fish (2004)
Bucio Galindo, A., Hartemink, R., Schrama, J.W., Rombouts, F.M.
Microbial changes during the production of Sufu - a Chinese fermented soybean food (2004)
Han, B., Cao, C.F., Rombouts, F.M., Nout, M.J.R.
Sufu is a Chinese soybean cheese obtained by solid-state fungal fermentation of tofu followed by ripening in dressing mixture. The aim of this study was to quantify microflora changes during the sufu...
Timmerman, H.M., Koning, C.J.M., Mulder, L., Rombouts, F.M., Beynen, A.C.
This literature review was carried out to make a comparison of functionality and efficacy between monostrain, multistrain and multispecies probiotics. A monostrain probiotic is defined as containing...
Membrane fluidity adjustments in ethanol-stressed Oenococcus oeni cells (2003)
Golovina, E.A., Hoekstra, F.A., Rombouts, F.M., Abee, T.
The effect of ethanol on the cytoplasmic membrane of Oenococcus oeni cells and the role of membrane changes in the acquired tolerance to ethanol were investigated. Membrane tolerance to ethanol was...
Han, B., Ma, Y., Rombouts, F.M., Nout, M.J.R.
Sufu is a Chinese soybean cheese obtained after maturation of solid-state mould-fermented tofu. Ambient temperatures of 30-35°C during the summer season prohibit the use of the usual starter...
Comparison of media for enumeration of Clostridium perfringens from foods (2003)
Eijhusen, G.P., Grand, M., Johansson, T., Kärkkäinen, T., ...
Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens...
Han, B., Rombouts, F.M., Nout, M.J.R.
Sufu is made by solid state fungal fermentation (using Actinomucor elegans) of tofu, followed by salting and maturation in dressing mixtures containing salt, alcohol and various other ingredients....
Effect of fermented soya beans on diarrhoea and feed efficiency in weaned piglets (2003)
Kiers, J.L., Meijer, J.C., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.
Aims: To evaluate anti-diarrhoeal and growth enhancing properties of fermented soya beans in weaned piglets. Methods and Results: In a first phase piglet diet, toasted full-fat soya beans (20€were...
Luppens, S.B.I., Barbaras, B., Breeuwer, P., Rombouts, F.M., Abee, T.
The aim of this study was to select fluorescence methods for use as alternatives to plate counting to assess the viability of Listeria monocytogenes cells exposed to benzalkonium chloride (BAC) and...
Kusumaningrum, H.D., Paltinaite, R., Koomen, A.J., Hazeleger, W.C., Rombouts, F.M., Beumer, R.R.
Effective cleaning and sanitizing of food preparation sites is important because pathogens are readily spread to food contact surfaces after preparation of contaminated raw products. Tolerance of...
Luppens, S.B.I., Rombouts, F.M., Abee, T.
The influence of growth phase on the resistance of Staphylococcus aureus to the surface-active agents benzalkonium chloride and dodecylbenzyl sulfonic acid and the oxidizing agents sodium...
Luppens, S.B.I., Reij, M.W., Rombouts, F.M., Abee, T.
A standardized disinfectant test for Staphylococcus aureus cells in biofilms was developed. Two disinfectants, the membrane-active compound benzalkonium chloride (BAC) and the oxidizing agent sodium...
Kusumaningrum, H.D., Rombouts, F.M., Beumer, R.R.
In response to increasing concern about home hygiene, the use of antibacterial products to reduce microorganisms in kitchen sponges and cleaning cloths is strongly promoted by some producers of...
Optimizing sporulation of Clostridium perfringens (2002)
Many sporulation media have been developed for Clostridium perfringens, but none stimulates sporulation for all strains. The aim of our experiments was to develop a sporulation method using Duncan...
Inhibition of adhesion of enterotoxigenic Escherichia coli K88 by soya bean tempe (2002)
Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.
Aims: Tempe is a traditional fungal fermented food made from soaked and cooked soya beans. It has been associated with antidiarrhoeal characteristics. This study investigated potential inhibitory...
Ben Amor, K., Breeuwer, P., Verbaarschot, P., Rombouts, F.M., Akkermans, A.D.L., ...
Using a flow cytometry-based approach, we assessed the viability of Bifidobacterium lactis DSM 10140 and Bifidobacterium adolescentis DSM 20083 during exposure to bile salt stress. Carboxyfluorescein...
Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells (2002)
San Romao, M.V., Loureiro-Dias, M.C., Rombouts, F.M., Abee, T.
The practical application of commercial malolactic starter cultures of Oenococcus oeni surviving direct inoculation in wine requires insight into the mechanisms involved in ethanol toxicity and...
Functionality assessment by flow cytometry (2002)
Bunthof, C.J., Breeuwer, P., Bloemen, K., Meijer, W., Hugenholtz, J., ...
A consumer-oriented classification system for home meal replacements (2001)
Costa, A.I.A., Dekker, M., Beumer, R.R., Rombouts, F.M., Jongen, W.M.F.
This paper introduces a new definition and classification system for home meal replacements (HMR), based on convenience attributes as viewed by consumers. An overview of other food classifications,...
A quantitative analysis of convenience-related food consumption in the Netherlands (2001)
Costa, A.I.A., Dekker, M., Beumer, R.R., Rombouts, F.M., Jongen, W.M.F.
Wijtzes, T., Rombouts, F.M., Zwietering, M.H.
A model was established to predict growth rate as a function of temperature, pH and water activity. The model is based on two, earlier developed models, one for growth rate as a function of...
Flow cytometric assessment of viability of lactic acid bacteria (2001)
Bunthof, C.J., Bloemen, K., Breeuwer, P., Rombouts, F.M., Abee, T.
The viability of lactic acid bacteria is crucial for their applications as dairy starters and as probiotics. We investigated the usefulness of flow cytometry (FCM) for viability assessment of lactic...
A quantitative analysis of convenience-related food consumption in The Netherlands (2001)
Costa, A.I.A., Dekker, M., Beumer, R.R., Rombouts, F.M., Jongen, W.M.F.
Process for the manufacture of a fermented health-promoting product (2001)
Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Koops, B.C., Wissing, E., ...
The present invention relates to a process that yields a product that can suitably be used in the treatment and prevention of diarrhea without giving rise to any undesirable side-effects. The...
Fluorescence assessment of Lactococcus lactis viability (2000)
Bunthof, C.J., Breeuwer, P., Rombouts, F.M., Abee, T.
The reproduction and activity of lactic acid bacteria (LAB) are essential in their applications in the dairy industry and other fermentations. Traditionally used methods like plate counting and...
The role of cold-shock proteins in low-temperature adaptation of food-related bacteria (2000)
Wouters, J.A., Rombouts, F.M., Kuipers, O.P., Abee, T.
There is a considerable interest in the cold adaptation of food-related bacteria, including starter cultures for industrial food fermentations, food spoilage bacteria and food-borne pathogens....
Wouters, J.A., Mailhes, M., Rombouts, F.M., Kuipers, O.P., Abee, T.
The physiological and regulatory effects of overproduction of five cold shock proteins (CSPs) of Lactococcus lactis were studied. CspB, CspD, and CspE could be overproduced at high levels (up to...
In vitro digestibility of processed and fermented soya bean, cowpea and maize (2000)
Kiers, J.L., Nout, M.J.R., Rombouts, F.M.
Tropical legumes, ie soya bean and cowpea, were pre-treated and subsequently fermented using pure cultures of Rhizopus spp. Impact of soaking, cooking and fermentation of the legumes on their...
Viability assessment of disinfectant stressed Listeria monocytogenes by flow cytometry (2000)
Luppens, S.B.I., Barbaras, B., Breeuwer, P., Abee, T., Rombouts, F.M.
Brouwer-Post, E., Rombouts, F.M., Abee, T.
Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor. This highly controllable system combines short contact times between yeast and wort with the...
A consumer-oriented classification for home meal replacements and its safety implications (1999)
Costa, A.I.A., Dekker, M., Beumer, R., Rombouts, F.M., Jongen, W.M.F.
Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor operating in downflow. This ensures a highly controllable system with optimal reactor design. In...
Food microbiology and food safety into the next millennium (1999)
Tuijtelaars, A.C.J., Samson, R.A., Rombouts, F.M., Notermans, S.
Incidence of Bacillus cereus and Bacillus subtilis in foods in the Netherlands. (1996)
Te Giffel, M.C., Beumer, R.R., Leijendekkers, S., Rombouts, F.M.
Incidence and characterization of Bacillus cereus in two dairy processing plants. (1996)
Te Giffel, M.C., Beumer, R.R., Bonestroo, M.H., Rombouts, F.M.
Listeria species in domestic environments. (1996)
Beumer, R.R., Te Giffel, M.C., Spoorenberg, E., Rombouts, F.M.
Use of starter cultures of lactobacilli and yeasts in the fermentation of mawe porridge. (1996)
Hounhouigan, D.J., Nout, M.J.R., Nago, C.M., Houben, J.H., Rombouts, F.M.
Method for preservation foodstuffs. (1996)
Bonestroo, M.H., Peelen, P.H.M., Kusters, B.J.M., Rombouts, F.M., Wit De, J.C.
A comparison of rapid methods for the detection of Listeria monocytogenes. (1996)
Beumer, R.R., Te Giffel, M.C., Kok, M.T.C., Cox, L.J., Rombouts, F.M.
A comparison of (rapid) methods for the detection of Listeria monocytogenes. (1995)
Beumer, R.R., Te Giffel, M.C., Kok, M.T.C., Cox, L.J., Rombouts, F.M.
Degradation and fermentation of fructo-oligosaccharides by oral streptococci. (1995)
Hartemink, R., Quataert, M.C.J., Nout, M.J.R., Rombouts, F.M.
Effect of exogenous betaine and carnitine on the growth of Listeria in a minimal medium. (1995)
Te Giffel, M.C., Beumer, R.R., Cox, L.J., Rombouts, F.M., Abee, T.
Role of peptide transport in the growth of Listeria monocytogenes. (1995)
Verheul, A., Hagting, A., Booth, I.R., Rombouts, F.M., Abee, T.
Microbiological changes in mawè during natural fermentation. (1994)
Hounhouigan, D.J., Nout, M.J.R., Nago, C.M., Houben, J.H., Rombouts, F.M.
Abee, T., Rombouts, F.M., Hugenholtz, J., Guihard, G., Letellier, L.
Nisin Z, a natural nisin variant, was recently isolated from Lactococcus lactis subspecies lactis NIZO 22186. The gene for this lantibiotic, designated nisZ, has been cloned, and its nucleotide...
Modelling growth rates of Listeria innocua as a function of lactate concentration. (1994)
Houtsma, P.C., Kusters, B.J.M., Rombouts, F.M., Zwietering, M.H.
Modelling the adsorption of endo-polygalacturonase on alginate beads. (1994)
Somers, W.A.C., Smolders, A.J.J., Beverloo, W.A., Rozie, H.J., Visser, J., Rombouts, F.M., ...
Alginate-starch co-polymers and immobilized starch as affinity adsorbents for _-amylase. (1993)
Somers, W.A.C., Kutsch Lojenga, A., Bonte, A., Rozie, H.J., Visser, J., Rombouts, F.M., ...
Changes in the physico-chemical properties of maize during natural fermentation of mawè. (1993)
Hounhouigan, D.J., Nout, M.J.R., Nago, C.M., Houben, J.H., Rombouts, F.M.
Oxygen scavenging in mayonnaise-based salads by lactic fermentation. (1993)
Rombouts, F.M., Bonestroo, M.H., Kusters, B.J.M., Cozijnsen, J.L.
The antimicrobial activity of sodium lactate. (1993)
Houtsma, P., Zwietering, M.H., Kusters, B.J.M., Rombouts, F.M.
Alginate-starch co-polymers and immobilized starch as affinity absorbent for _-amylase. (1993)
Somers, W.A., Kutsch Lojenga, A., Bonte, A., Rozie, H.J., Visser, J., Rombouts, F.M., ...
Immuno-assays for specific detection of moulds of the order Mucorales and the genus Botrytis. (1992)
Production and quality of maize-based fermented dough in Benin urban area. (1992)
Hounhouigan, D.J., Jansen, J.M.M., Nout, M.J.R., Nago, M.C., Rombouts, F.M.
Crosslinked xylan as an affinity adsorbent for endo-xylanases. (1992)
Rozie, H., Somers, W., Bonte, A., Rombouts, F.M., Visser, J.
Adsorption characteristics of bacterial _-amylases on cross-linked potato starch. (1991)
Rozie, H., Somers, W., Bonte, A., Visser, J., Rombouts, F.M.
On the interaction of _-amylase with cross-linked starch. Evaluation of process conditions. (1991)
Somers, W., Rozie, H., Bonte, A., Visser, J., Rombouts, F.M.
Extracellular polysaccharides of moulds and their immunological activity. (1991)
Kamphuis, H.J., Notermans, S.H.W., Veeneman, G.H., Rombouts, F.M.
Crosslinked xylan as an affinity adsorbent for endo-xylanases. (1991)
Rozie, H., Somers, W., Bonte, A., Rombouts, F.M., Visser, J.
Bonestroo, M.H., Peelen, P.H.M., Kusters, B.J.M., Rombouts, F.M.
The invention relates to a method for preserving foodstuffs, with which method foodstuffs are inoculated with an amount of lactic acid-forming bacteria and/or parts of bacteria and these bacteria...
On the interaction of _-amylase with crosslinked starch: evaluation of process conditions. (1991)
Somers, W., Rozie, H., Bonte, A., Visser, J., Rombouts, F.M.
Bonestroo, M.H., Kusters, B.J.M., Peelen, P.H.M., Rombouts, F.M.
The invention relates to a method for preserving foodstuffs, with which method foodstuffs are inoculated with an amount of lactic acid-forming bacteria and/or parts of bacteria and these bacteria...
Adsorption characteristics of endo-polygalacturonase on alginate beads. (1989)
Rozie, H., Somers, W., Bonte, A., Visser, J., Rombouts, F.M.
Microbiële risico's van de voeding. (1989)
Rombouts, F.M., Beumer, R.R., Nout, M.J.R., Notermans, S.H.W.
Enzymic hydrolysis of the 'hairy' fragments of sugarbeet pectins. (1989)
Guillon, F., Thibault, J.F., Rombouts, F.M., Voragen, A.G.J., Pilnik, W.
Effect of temperature, O2 and CO2 on Rhizopus oligosporus biomass production. (1988)
Nout, M.J.R., Baltus, F.J.A., Zwietering, M.H., Rombouts, F.M.
Oxidative enzyme-catalyzed crosslinking of beet pectins. (1987)
Rombouts, F.M., Thibault, J.F., Mercier, C.
Beet pectin is crosslinked with an oxidizing system containing an oxidizing agent and an enzyme such as peroxidase that uses the oxidizing agent as a substrate. The crosslinked beet pectin is useful...
Structural features of the neutral sugar side chains of beet pulp pectins. (1986)
Guillon, F., Thibault, J.F., Rombouts, F.M., Voragen, A.G.J., Pilnik, W.
Enzymatic Hydrolysis of Naringin in Grapefruit Juice (1977)
Versteeg, C., Martens, L.J.H., Rombouts, F.M., Voragen, A.G.J., Pilnik, W.
Occurrence and properties of bacterial pectate lyases / (1972)
"Stellingen inserted."
Verheul, A, Hagting, A, Amezaga, M R, Booth, I R, Rombouts, F M, Abee, T
Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and...
Breeuwer, P, Drocourt, J L, Bunschoten, N, Zwietering, M H, Rombouts, F M, Abee, T
Flow cytometry is a rapid and sensitive method which may be used for the detection of microorganisms in foods and drinks. A key requirement for this method is a sufficient fluorescence staining of...
Hazeleger, W C, Janse, J D, Koenraad, P M, Beumer, R R, Rombouts, F M, Abee, T
The effect of temperature and the availability of nutrients on the transition of spiral Campylobacter jejuni cells to coccoid forms was investigated. Ageing of spiral C. jejuni cells in either...
Coffey, A, Rombouts, F M, Abee, T
The ability to produce phosphatidylcholine phospholipase C (lecithinase) is associated with virulence in pathogenic species of Listeria. Levels of production vary greatly among members of the genus,...
Houtsma, P C, Kant-Muermans, M L, Rombouts, F M, Zwietering, M H
A modified Monod equation was successfully applied to describe the maximum specific growth rate of Listeria innocua in a broth model in the presence of various concentrations of sodium lactate or...
Verheul, A, Russell, N J, Van'T Hof, R, Rombouts, F M, Abee, T
A nisin-resistant (NISr) variant of Listeria monocytogenes Scott A was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (BHI) broth. The NISr strain was...
Van Iersel, M F, Eppink, M H, Van Berkel, W J, Rombouts, F M, Abee, T
An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a...
Verheul, A, Rombouts, F M, Beumer, R R, Abee, T
Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can...
Modeling of the Bacterial Growth Curve
Zwietering, M. H., Jongenburger, I., Rombouts, F. M.
Several sigmoidal functions (logistic, Gompertz, Richards, Schnute, and Stannard) were compared to describe a bacterial growth curve. They were compared statistically by using the model of Schnute,...
Beumer, R R, Te Giffel, M C, Cox, L J, Rombouts, F M, Abee, T
Three Listeria monocytogenes strains isolated from food or food-processing environments were used to assess the response of this species to salinity in a chemically defined minimal medium. Growth in...
Breeuwer, P, Drocourt, J L, Rombouts, F M, Abee, T
Carboxyfluorescein diacetate is a nonfluorescent compound which can be used in combination with flow cytometry for vital staining of yeasts and bacteria. The basis of this method is the assumption...
Kamphuis, H J, De Ruiter, G A, Mischnick, P, Van Boom, J H, Rombouts, F M
To study the structures of the epitopes of the extracellular polysaccharides from Penicillium and Aspergillus species, an exo-beta-D-galactofuranosidase was purified from a commercial crude enzyme...
Breeuwer, P., Drocourt, J., Rombouts, F. M., Abee, T.
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria....
Breeuwer, P., De Reu, J. C., Drocourt, J., Rombouts, F. M., Abee, T.
Germination of Rhizopus oligosporus sporangiospores is characterized by swelling of the spores and subsequent emergence of germ tubes. Changes in spore morphology and alterations in intracellular pH...
Verheul, A, Hagting, A, Amezaga, M R, Booth, I R, Rombouts, F M, Abee, T
Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and...
Breeuwer, P, Drocourt, J L, Bunschoten, N, Zwietering, M H, Rombouts, F M, Abee, T
Flow cytometry is a rapid and sensitive method which may be used for the detection of microorganisms in foods and drinks. A key requirement for this method is a sufficient fluorescence staining of...
Hazeleger, W C, Janse, J D, Koenraad, P M, Beumer, R R, Rombouts, F M, Abee, T
The effect of temperature and the availability of nutrients on the transition of spiral Campylobacter jejuni cells to coccoid forms was investigated. Ageing of spiral C. jejuni cells in either...
Coffey, A, Rombouts, F M, Abee, T
The ability to produce phosphatidylcholine phospholipase C (lecithinase) is associated with virulence in pathogenic species of Listeria. Levels of production vary greatly among members of the genus,...
Houtsma, P C, Kant-Muermans, M L, Rombouts, F M, Zwietering, M H
A modified Monod equation was successfully applied to describe the maximum specific growth rate of Listeria innocua in a broth model in the presence of various concentrations of sodium lactate or...
Verheul, A, Russell, N J, Van'T Hof, R, Rombouts, F M, Abee, T
A nisin-resistant (NISr) variant of Listeria monocytogenes Scott A was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (BHI) broth. The NISr strain was...
Van Iersel, M F, Eppink, M H, Van Berkel, W J, Rombouts, F M, Abee, T
An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a...
Verheul, A, Rombouts, F M, Beumer, R R, Abee, T
Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can...
Modeling of the Bacterial Growth Curve
Zwietering, M. H., Jongenburger, I., Rombouts, F. M.
Several sigmoidal functions (logistic, Gompertz, Richards, Schnute, and Stannard) were compared to describe a bacterial growth curve. They were compared statistically by using the model of Schnute,...
Beumer, R R, Te Giffel, M C, Cox, L J, Rombouts, F M, Abee, T
Three Listeria monocytogenes strains isolated from food or food-processing environments were used to assess the response of this species to salinity in a chemically defined minimal medium. Growth in...
Breeuwer, P, Drocourt, J L, Rombouts, F M, Abee, T
Carboxyfluorescein diacetate is a nonfluorescent compound which can be used in combination with flow cytometry for vital staining of yeasts and bacteria. The basis of this method is the assumption...
Kamphuis, H J, De Ruiter, G A, Mischnick, P, Van Boom, J H, Rombouts, F M
To study the structures of the epitopes of the extracellular polysaccharides from Penicillium and Aspergillus species, an exo-beta-D-galactofuranosidase was purified from a commercial crude enzyme...
Breeuwer, P., Drocourt, J., Rombouts, F. M., Abee, T.
A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria....
Breeuwer, P., De Reu, J. C., Drocourt, J., Rombouts, F. M., Abee, T.
Germination of Rhizopus oligosporus sporangiospores is characterized by swelling of the spores and subsequent emergence of germ tubes. Changes in spore morphology and alterations in intracellular pH...