F. M. Rombouts

Publication List Details

Period

1972 - 2008

Number

477

Co-Authors

The effect of a multispecies probiotic on the intestinal microbiota and bowel movements in healthy volunteers taking the antibiotic amoxycillin (2008)

Koning, C.J.M., Stobberingh, E.E., Mulder, L., Rombouts, F.M., Stockbrügger, R.W.

BACKGROUND: One of the side effects of antimicrobial therapy is a disturbance of the intestinal microbiota potentially resulting in antibiotic-associated diarrhea (AAD). In this placebo-controlled...

Characteristics of some traditional Vietnamese starch-based rice wine fermentation starters (men) (2007)

Dung, N.T.P., Rombouts, F.M., Nout, M.J.R.

In the Mekong Delta region of South-Vietnam, wine from purple glutinous rice is particularly interesting because of its sherry-like taste and flavour and its attractive brown-red colour. It is...

Viability and physiological state transitions of Rhizopus oligosporus sporangiospores in tempe starter culture (2007)

Thanh, N.V., Rombouts, F.M., Nout, M.J.R.

The viability and various physiological characteristics of individual sporangiospores of Rhizopus oligosporus in tempe starter cultures that had been stored for 8, 10, 16 and 30 months were examined...

A high molecular weight soluble fraction of tempeh protects against fluid losses in Escherichia coli-infected piglet small intestine (2007)

Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.

Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhoea in children and piglets. Infection of ETEC results in fluid secretion and electrolyte losses in the small intestine. In this...

Design of a multispecies probiotic mixture to prevent infectious complications in critically ill patients (2007)

Timmerman, H.M., Niers, L.E.M., Ridwan, B.U., Koning, C.J.M., Mulder, L., Akkermans, L.M.A., ...

Background & aims Although the potential for probiotics is investigated in an increasing variety of diseases, there is little or no consensus regarding the desired probiotic properties for a...

Nutritional support for the infant's immune system (2007)

Niers, L., Stasse-Wolthuis, M., Rombouts, F.M., Rijkers, G.T.

Newborn babies possess a functional but immature immune system as a defense against a world teeming with microorganisms. Breast milk contains a number of biological, active compounds that support the...

Preservation of blue-jack mackerel (Trachurus picturatus Bowdich) silage by chemical and fermentative acidification (2007)

Nout, M.J.R., Rombouts, F.M., Houben, J.H.

We compared acidified and lactic acid fermented silage approaches for the preservation of blue-jack mackerel. Silages acidified with formic and propionic acids had stable pH (3.8) and low (19 mg/g N)...

Presence of lactobacilli in the intestinal content of freshwater fish from a river and from a farm with a recirculation system (2006)

Bucio Galindo, A., Hartemink, R., Schrama, J.W., Verreth, J.A.J., Rombouts, F.M.

Lactobacilli are Gram-positive and catalase negative rods commonly found in lactic acid fermented foods and in the gastrointestinal tract of mammals and birds. Few studies have described lactobacilli...

Effect of processed and fermented soyabeans on net absorption in enterotoxigenic Escherichia coli-infected piglet small intestine (2006)

Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.

Infectious diarrhoea is a major problem in both children and piglets. Infection of enterotoxigenic Escherichia coli (ETEC) results in fluid secretion and electrolyte losses in the small intestine. In...

Mortality and growth performance of broilers given drinking water supplemented with chicken-specific probiotics (2006)

Timmerman, H.M., Veldman, A., Rombouts, F.M., Beynen, A.C.

For application in broiler production, we developed a multispecies (MSPB) and a chicken-specific (CSPB) probiotic preparation in fluid form. The MSPB contained different probiotic species of human...

Functionality of selected strains of moulds and yeasts from Vietnamese rice wine starters (2006)

Dung, N.T.P., Rombouts, F.M., Nout, M.J.R.

The role of starch-degrading mycelial fungi, and the alcohol production and ethanol tolerance of the yeasts isolated from selected Vietnamese traditional rice wine starters were examined, and optimum...

Effect of osmolality on net fluid absorption in non-infected and ETEC-infected piglet small intestinal segments (2006)

Kiers, J.L., Hoogendoorn, A., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.

In the small intestinal segment perfusion model the effect of osmolality on net fluid absorption in enterotoxigenic Escherichia coli (ETEC)-infected and non-infected small intestinal segments of...

Prevalence of potentially pathogenic Bacillus cereus in food commodities in The Netherlands (2006)

Wijnands, L.M., Dufrenne, J.B., Rombouts, F.M.

Randomly selected food commodities, categorized in product groups, were investigated for the presence and number of Bacillus cereus bacteria. If positive, and when possible, five separate colonies...

Survival of Lactobacillus plantarum 44a after spraying and drying in feed and during exposure to gastrointestinal tract fluids in vitro (2005)

Bucio Galindo, A., Hartemink, R., Schrama, J.W., Verreth, J.A.J., Rombouts, F.M.

A good probiotic strain should be able to survive the conditions of handling and storage to be delivered in high concentration to the host. That is especially important when stressful conditions are...

Differentiation inside multicelled macroconidia of Fusarium culmorum during early germination (2005)

Chitarra, G.S., Breeuwer, P., Rombouts, F.M., Abee, T., Dijksterhuis, J.

Multicelled conidia are formed by many fungal species, but germination of these spores is scarcely studied. Here, the germination and the effects of antimicrobials on multicompartment macroconidia of...

Development of defined mixed-culture fungal fermentation starter granulate for controlled production of rice wine (2005)

Rombouts, F.M., Nout, M.J.R.

As a first step in the development of defined fungal starter granules for controlled winemaking from purple glutinous rice, the interaction of moulds and yeasts isolated from Vietnamese rice wine...

Health and growth of veal calves fed milk replacers with or without probiotics (2005)

Timmerman, H.M., Mulder, L., Everts, H., Klaassen, G., ...

Four experiments with 1-wk-old veal calves were conducted to assess the influence of probiotics on growth and health indicators. In experiments 1 and 2, the liquid probiotic supplements were...

Position paper on probiotics (2005)

Tijsseling, I.A., Pekelharing, P.R., Rombouts, F.M.

Probiotics are live microorganisms which when administered in adequate amounts confer a health benefit on the host. Probiotics, used to supplement normal daily nutrition, are therefore an important...

Gut flora in health and disease : potential role of probiotics (2005)

Heselmans, M., Reid, G., Akkermans, L.M.A., Savelkoul, H.F.J., Timmerman, H., Rombouts, F.M.

In a young evolving science, there are always more questions than answers. That is also the situation in the emerging field of Probiotics, and this was made very clear at the International Probiotics...

1-Octen-3-ol inhibits conidia germination of Penicillium paneum despite of mild effects on membrane permeability, respiration, intracellular pH, and changes the protein composition (2005)

Chitarra, G.S., Abee, T., Rombouts, F.M., Dijksterhuis, J.

1-Octen-3-ol is a volatile germination self-inhibitor produced by Penicillium paneum that blocks the germination process. The size of conidia treated with 1-octen-3-ol was similar to that of freshly...

Microbial changes during the production of Sufu - a Chinese fermented soybean food (2004)

Han, B., Cao, C.F., Rombouts, F.M., Nout, M.J.R.

Sufu is a Chinese soybean cheese obtained by solid-state fungal fermentation of tofu followed by ripening in dressing mixture. The aim of this study was to quantify microflora changes during the sufu...

Monostrain, multistrain and multispecies probiotics - A comparison of functionality and efficacy (2004)

Timmerman, H.M., Koning, C.J.M., Mulder, L., Rombouts, F.M., Beynen, A.C.

This literature review was carried out to make a comparison of functionality and efficacy between monostrain, multistrain and multispecies probiotics. A monostrain probiotic is defined as containing...

Membrane fluidity adjustments in ethanol-stressed Oenococcus oeni cells (2003)

Golovina, E.A., Hoekstra, F.A., Rombouts, F.M., Abee, T.

The effect of ethanol on the cytoplasmic membrane of Oenococcus oeni cells and the role of membrane changes in the acquired tolerance to ethanol were investigated. Membrane tolerance to ethanol was...

Effects of temperature and relative humidity on growth and enzyme production by Actinomucor elegans and Rhizopus oligosporus during sufu pehtze preparation (2003)

Han, B., Ma, Y., Rombouts, F.M., Nout, M.J.R.

Sufu is a Chinese soybean cheese obtained after maturation of solid-state mould-fermented tofu. Ambient temperatures of 30-35°C during the summer season prohibit the use of the usual starter...

Comparison of media for enumeration of Clostridium perfringens from foods (2003)

Eijhusen, G.P., Grand, M., Johansson, T., Kärkkäinen, T., ...

Many media have been developed to enumerate Clostridium perfringens from foods. In this study, six media [iron sulfite (IS) agar, tryptose sulfite cycloserine (TSC) agar, Shahidi Ferguson perfringens...

Effect of NaCl on textural changes and protein and lipid degradation during the ripening stage of sufu, a Chinese fermented soybean food (2003)

Han, B., Rombouts, F.M., Nout, M.J.R.

Sufu is made by solid state fungal fermentation (using Actinomucor elegans) of tofu, followed by salting and maturation in dressing mixtures containing salt, alcohol and various other ingredients....

Effect of fermented soya beans on diarrhoea and feed efficiency in weaned piglets (2003)

Kiers, J.L., Meijer, J.C., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.

Aims: To evaluate anti-diarrhoeal and growth enhancing properties of fermented soya beans in weaned piglets. Methods and Results: In a first phase piglet diet, toasted full-fat soya beans (20€were...

Selection of fluorescent probes for flow cytometric viability assessment of Listeria monocytogenes exposed to membrane-active and oxidizing disinfectants (2003)

Luppens, S.B.I., Barbaras, B., Breeuwer, P., Rombouts, F.M., Abee, T.

The aim of this study was to select fluorescence methods for use as alternatives to plate counting to assess the viability of Listeria monocytogenes cells exposed to benzalkonium chloride (BAC) and...

Tolerance of Salmonella Enteritidis and Staphylococcus aureus to surface cleaning and household bleach (2003)

Kusumaningrum, H.D., Paltinaite, R., Koomen, A.J., Hazeleger, W.C., Rombouts, F.M., Beumer, R.R.

Effective cleaning and sanitizing of food preparation sites is important because pathogens are readily spread to food contact surfaces after preparation of contaminated raw products. Tolerance of...

The effect of the Growth Phase of Staphylococcus aureus on Resistance to Disinfectants in a Suspension Test (2002)

Luppens, S.B.I., Rombouts, F.M., Abee, T.

The influence of growth phase on the resistance of Staphylococcus aureus to the surface-active agents benzalkonium chloride and dodecylbenzyl sulfonic acid and the oxidizing agents sodium...

Development of a Standard Test to Assess the Resistance of Staphylococcus aureus Biofilm Cells to Disinfectants (2002)

Luppens, S.B.I., Reij, M.W., Rombouts, F.M., Abee, T.

A standardized disinfectant test for Staphylococcus aureus cells in biofilms was developed. Two disinfectants, the membrane-active compound benzalkonium chloride (BAC) and the oxidizing agent sodium...

Effects of antibacterial dishwashing liquid on foodborne pathogens and competitive microorganisms in kitchen sponges (2002)

Kusumaningrum, H.D., Rombouts, F.M., Beumer, R.R.

In response to increasing concern about home hygiene, the use of antibacterial products to reduce microorganisms in kitchen sponges and cleaning cloths is strongly promoted by some producers of...

Optimizing sporulation of Clostridium perfringens (2002)

Beumer, R.R., Rombouts, F.M.

Many sporulation media have been developed for Clostridium perfringens, but none stimulates sporulation for all strains. The aim of our experiments was to develop a sporulation method using Duncan...

Inhibition of adhesion of enterotoxigenic Escherichia coli K88 by soya bean tempe (2002)

Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Nabuurs, M.J.A.

Aims: Tempe is a traditional fungal fermented food made from soaked and cooked soya beans. It has been associated with antidiarrhoeal characteristics. This study investigated potential inhibitory...

Multiparametric flow cytometry and cell sorting for the assessment of viable, injured, and dead bifidobacterium cells during bile salt stress (2002)

Ben Amor, K., Breeuwer, P., Verbaarschot, P., Rombouts, F.M., Akkermans, A.D.L., ...

Using a flow cytometry-based approach, we assessed the viability of Bifidobacterium lactis DSM 10140 and Bifidobacterium adolescentis DSM 20083 during exposure to bile salt stress. Carboxyfluorescein...

Flow cytometric assessment of membrane integrity of ethanol-stressed Oenococcus oeni cells (2002)

San Romao, M.V., Loureiro-Dias, M.C., Rombouts, F.M., Abee, T.

The practical application of commercial malolactic starter cultures of Oenococcus oeni surviving direct inoculation in wine requires insight into the mechanisms involved in ethanol toxicity and...

A consumer-oriented classification system for home meal replacements (2001)

Costa, A.I.A., Dekker, M., Beumer, R.R., Rombouts, F.M., Jongen, W.M.F.

This paper introduces a new definition and classification system for home meal replacements (HMR), based on convenience attributes as viewed by consumers. An overview of other food classifications,...

Development and validation of a combined temperature, water activity, pH model for bacterial growth rate of Lactobacillus curvatus (2001)

Wijtzes, T., Rombouts, F.M., Zwietering, M.H.

A model was established to predict growth rate as a function of temperature, pH and water activity. The model is based on two, earlier developed models, one for growth rate as a function of...

Flow cytometric assessment of viability of lactic acid bacteria (2001)

Bunthof, C.J., Bloemen, K., Breeuwer, P., Rombouts, F.M., Abee, T.

The viability of lactic acid bacteria is crucial for their applications as dairy starters and as probiotics. We investigated the usefulness of flow cytometry (FCM) for viability assessment of lactic...

Process for the manufacture of a fermented health-promoting product (2001)

Kiers, J.L., Nout, M.J.R., Rombouts, F.M., Koops, B.C., Wissing, E., ...

The present invention relates to a process that yields a product that can suitably be used in the treatment and prevention of diarrhea without giving rise to any undesirable side-effects. The...

Fluorescence assessment of Lactococcus lactis viability (2000)

Bunthof, C.J., Breeuwer, P., Rombouts, F.M., Abee, T.

The reproduction and activity of lactic acid bacteria (LAB) are essential in their applications in the dairy industry and other fermentations. Traditionally used methods like plate counting and...

The role of cold-shock proteins in low-temperature adaptation of food-related bacteria (2000)

Wouters, J.A., Rombouts, F.M., Kuipers, O.P., Abee, T.

There is a considerable interest in the cold adaptation of food-related bacteria, including starter cultures for industrial food fermentations, food spoilage bacteria and food-borne pathogens....

Physiological and regulatory effects of controlled overproduction of five cold shock proteins of Lactococcus lactis (2000)

Wouters, J.A., Mailhes, M., Rombouts, F.M., Kuipers, O.P., Abee, T.

The physiological and regulatory effects of overproduction of five cold shock proteins (CSPs) of Lactococcus lactis were studied. CspB, CspD, and CspE could be overproduced at high levels (up to...

In vitro digestibility of processed and fermented soya bean, cowpea and maize (2000)

Kiers, J.L., Nout, M.J.R., Rombouts, F.M.

Tropical legumes, ie soya bean and cowpea, were pre-treated and subsequently fermented using pure cultures of Rhizopus spp. Impact of soaking, cooking and fermentation of the legumes on their...

Influence of yeast immobilization on fermentation and aldehyde reduction during the production of alcohol-free beer (2000)

Brouwer-Post, E., Rombouts, F.M., Abee, T.

Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor. This highly controllable system combines short contact times between yeast and wort with the...

Flavor formation and cell physiology during the production of alcohol-free beer with immobilized Saccharomyces cerevisiae (1999)

Rombouts, F.M., Abee, T.

Production of alcohol-free beer by limited fermentation is optimally performed in a packed-bed reactor operating in downflow. This ensures a highly controllable system with optimal reactor design. In...

Mode of action of nisin Z against Listeria monocytogenes Scott A grown at high and low temperatures. (1994)

Abee, T., Rombouts, F.M., Hugenholtz, J., Guihard, G., Letellier, L.

Nisin Z, a natural nisin variant, was recently isolated from Lactococcus lactis subspecies lactis NIZO 22186. The gene for this lantibiotic, designated nisZ, has been cloned, and its nucleotide...

Werkwijze voor het verduurzamen van voedingsmiddelen, alsmede voedingsmiddelen verkregen onder toepassing van deze werkwijze. (1991)

Bonestroo, M.H., Peelen, P.H.M., Kusters, B.J.M., Rombouts, F.M.

The invention relates to a method for preserving foodstuffs, with which method foodstuffs are inoculated with an amount of lactic acid-forming bacteria and/or parts of bacteria and these bacteria...

Werkwijze voor het verduurzamen van voedingsmiddelen, alsmede voedingsmiddelen verkregen onder toepassing van deze werkwijze. (1990)

Bonestroo, M.H., Kusters, B.J.M., Peelen, P.H.M., Rombouts, F.M.

The invention relates to a method for preserving foodstuffs, with which method foodstuffs are inoculated with an amount of lactic acid-forming bacteria and/or parts of bacteria and these bacteria...

Oxidative enzyme-catalyzed crosslinking of beet pectins. (1987)

Rombouts, F.M., Thibault, J.F., Mercier, C.

Beet pectin is crosslinked with an oxidizing system containing an oxidizing agent and an enzyme such as peroxidase that uses the oxidizing agent as a substrate. The crosslinked beet pectin is useful...

A di- and tripeptide transport system can supply Listeria monocytogenes Scott A with amino acids essential for growth.

Verheul, A, Hagting, A, Amezaga, M R, Booth, I R, Rombouts, F M, Abee, T

Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and...

Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product.

Breeuwer, P, Drocourt, J L, Bunschoten, N, Zwietering, M H, Rombouts, F M, Abee, T

Flow cytometry is a rapid and sensitive method which may be used for the detection of microorganisms in foods and drinks. A key requirement for this method is a sufficient fluorescence staining of...

Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni.

Hazeleger, W C, Janse, J D, Koenraad, P M, Beumer, R R, Rombouts, F M, Abee, T

The effect of temperature and the availability of nutrients on the transition of spiral Campylobacter jejuni cells to coccoid forms was investigated. Ageing of spiral C. jejuni cells in either...

Influence of environmental parameters on phosphatidylcholine phospholipase C production in Listeria monocytogenes: a convenient method to differentiate L. monocytogenes from other Listeria species.

Coffey, A, Rombouts, F M, Abee, T

The ability to produce phosphatidylcholine phospholipase C (lecithinase) is associated with virulence in pathogenic species of Listeria. Levels of production vary greatly among members of the genus,...

Model for the combined effects of temperature, pH, and sodium lactate on growth rates of Listeria innocua in broth and Bologna-type sausages.

Houtsma, P C, Kant-Muermans, M L, Rombouts, F M, Zwietering, M H

A modified Monod equation was successfully applied to describe the maximum specific growth rate of Listeria innocua in a broth model in the presence of various concentrations of sodium lactate or...

Modifications of membrane phospholipid composition in nisin-resistant Listeria monocytogenes Scott A.

Verheul, A, Russell, N J, Van'T Hof, R, Rombouts, F M, Abee, T

A nisin-resistant (NISr) variant of Listeria monocytogenes Scott A was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (BHI) broth. The NISr strain was...

Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.

Van Iersel, M F, Eppink, M H, Van Berkel, W J, Rombouts, F M, Abee, T

An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a...

An ATP-dependent L-carnitine transporter in Listeria monocytogenes Scott A is involved in osmoprotection.

Verheul, A, Rombouts, F M, Beumer, R R, Abee, T

Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can...

Modeling of the Bacterial Growth Curve

Zwietering, M. H., Jongenburger, I., Rombouts, F. M.

Several sigmoidal functions (logistic, Gompertz, Richards, Schnute, and Stannard) were compared to describe a bacterial growth curve. They were compared statistically by using the model of Schnute,...

Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in a minimal medium.

Beumer, R R, Te Giffel, M C, Cox, L J, Rombouts, F M, Abee, T

Three Listeria monocytogenes strains isolated from food or food-processing environments were used to assess the response of this species to salinity in a chemically defined minimal medium. Growth in...

Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.

Breeuwer, P, Drocourt, J L, Rombouts, F M, Abee, T

Carboxyfluorescein diacetate is a nonfluorescent compound which can be used in combination with flow cytometry for vital staining of yeasts and bacteria. The basis of this method is the assumption...

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase.

Kamphuis, H J, De Ruiter, G A, Mischnick, P, Van Boom, J H, Rombouts, F M

To study the structures of the epitopes of the extracellular polysaccharides from Penicillium and Aspergillus species, an exo-beta-D-galactofuranosidase was purified from a commercial crude enzyme...

A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester

Breeuwer, P., Drocourt, J., Rombouts, F. M., Abee, T.

A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria....

Nonanoic Acid, a Fungal Self-Inhibitor, Prevents Germination of Rhizopus oligosporus Sporangiospores by Dissipation of the pH Gradient

Breeuwer, P., De Reu, J. C., Drocourt, J., Rombouts, F. M., Abee, T.

Germination of Rhizopus oligosporus sporangiospores is characterized by swelling of the spores and subsequent emergence of germ tubes. Changes in spore morphology and alterations in intracellular pH...

A di- and tripeptide transport system can supply Listeria monocytogenes Scott A with amino acids essential for growth.

Verheul, A, Hagting, A, Amezaga, M R, Booth, I R, Rombouts, F M, Abee, T

Listeria monocytogenes takes up di- and tripeptides via a proton motive force-dependent carrier protein. This peptide transport system resembles the recently cloned and sequenced secondary di- and...

Characterization of uptake and hydrolysis of fluorescein diacetate and carboxyfluorescein diacetate by intracellular esterases in Saccharomyces cerevisiae, which result in accumulation of fluorescent product.

Breeuwer, P, Drocourt, J L, Bunschoten, N, Zwietering, M H, Rombouts, F M, Abee, T

Flow cytometry is a rapid and sensitive method which may be used for the detection of microorganisms in foods and drinks. A key requirement for this method is a sufficient fluorescence staining of...

Temperature-dependent membrane fatty acid and cell physiology changes in coccoid forms of Campylobacter jejuni.

Hazeleger, W C, Janse, J D, Koenraad, P M, Beumer, R R, Rombouts, F M, Abee, T

The effect of temperature and the availability of nutrients on the transition of spiral Campylobacter jejuni cells to coccoid forms was investigated. Ageing of spiral C. jejuni cells in either...

Influence of environmental parameters on phosphatidylcholine phospholipase C production in Listeria monocytogenes: a convenient method to differentiate L. monocytogenes from other Listeria species.

Coffey, A, Rombouts, F M, Abee, T

The ability to produce phosphatidylcholine phospholipase C (lecithinase) is associated with virulence in pathogenic species of Listeria. Levels of production vary greatly among members of the genus,...

Model for the combined effects of temperature, pH, and sodium lactate on growth rates of Listeria innocua in broth and Bologna-type sausages.

Houtsma, P C, Kant-Muermans, M L, Rombouts, F M, Zwietering, M H

A modified Monod equation was successfully applied to describe the maximum specific growth rate of Listeria innocua in a broth model in the presence of various concentrations of sodium lactate or...

Modifications of membrane phospholipid composition in nisin-resistant Listeria monocytogenes Scott A.

Verheul, A, Russell, N J, Van'T Hof, R, Rombouts, F M, Abee, T

A nisin-resistant (NISr) variant of Listeria monocytogenes Scott A was isolated by stepwise exposure to increasing concentrations of nisin in brain heart infusion (BHI) broth. The NISr strain was...

Purification and characterization of a novel NADP-dependent branched-chain alcohol dehydrogenase from Saccharomyces cerevisiae.

Van Iersel, M F, Eppink, M H, Van Berkel, W J, Rombouts, F M, Abee, T

An NADP-dependent branched-chain alcohol dehydrogenase was purified from Saccharomyces cerevisiae var. uvarum grown under anaerobic conditions. Its quaternary structure is monomeric, and it has a...

An ATP-dependent L-carnitine transporter in Listeria monocytogenes Scott A is involved in osmoprotection.

Verheul, A, Rombouts, F M, Beumer, R R, Abee, T

Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can...

Modeling of the Bacterial Growth Curve

Zwietering, M. H., Jongenburger, I., Rombouts, F. M.

Several sigmoidal functions (logistic, Gompertz, Richards, Schnute, and Stannard) were compared to describe a bacterial growth curve. They were compared statistically by using the model of Schnute,...

Effect of exogenous proline, betaine, and carnitine on growth of Listeria monocytogenes in a minimal medium.

Beumer, R R, Te Giffel, M C, Cox, L J, Rombouts, F M, Abee, T

Three Listeria monocytogenes strains isolated from food or food-processing environments were used to assess the response of this species to salinity in a chemically defined minimal medium. Growth in...

Energy-dependent, carrier-mediated extrusion of carboxyfluorescein from Saccharomyces cerevisiae allows rapid assessment of cell viability by flow cytometry.

Breeuwer, P, Drocourt, J L, Rombouts, F M, Abee, T

Carboxyfluorescein diacetate is a nonfluorescent compound which can be used in combination with flow cytometry for vital staining of yeasts and bacteria. The basis of this method is the assumption...

New structural features of the antigenic extracellular polysaccharides of Penicillium and Aspergillus species revealed with exo-beta-D-galactofuranosidase.

Kamphuis, H J, De Ruiter, G A, Mischnick, P, Van Boom, J H, Rombouts, F M

To study the structures of the epitopes of the extracellular polysaccharides from Penicillium and Aspergillus species, an exo-beta-D-galactofuranosidase was purified from a commercial crude enzyme...

A Novel Method for Continuous Determination of the Intracellular pH in Bacteria with the Internally Conjugated Fluorescent Probe 5 (and 6-)-Carboxyfluorescein Succinimidyl Ester

Breeuwer, P., Drocourt, J., Rombouts, F. M., Abee, T.

A novel method based on the intracellular conjugation of the fluorescent probe 5 (and 6-)-carboxyfluorescein succinimidyl ester (cFSE) was developed to determine the intracellular pH of bacteria....

Nonanoic Acid, a Fungal Self-Inhibitor, Prevents Germination of Rhizopus oligosporus Sporangiospores by Dissipation of the pH Gradient

Breeuwer, P., De Reu, J. C., Drocourt, J., Rombouts, F. M., Abee, T.

Germination of Rhizopus oligosporus sporangiospores is characterized by swelling of the spores and subsequent emergence of germ tubes. Changes in spore morphology and alterations in intracellular pH...