A Possible Role for the Adenylcyclase System in Insulin Secretion*
Malaisse, W. J., Malaisse-Lagae, F., Mayhew, D.
A possible role for adenylcyclase in insulin secretion was investigated. Isoproterenol, a predominantly β-adrenergic agent, when mixed with an α-adrenergic blocking agent (phenoxybenzamine),...
Orci, L, Malaisse-Lagae, F, Ravazzola, M, Rouiller, D, Renold, A E, Perrelet, A, ...
By degranulating beta-cells in the islets of Langerhans of the rat with sulfonylurea, it has been possible to distinguish unambiguously alpha-cells from beta-cells in freeze-fracture replicas. In...
Dynamics of Insulin Release and Microtubular-Microfilamentous System. I. EFFECT OF CYTOCHALASIN B
Van Obberghen, E., Somers, G., Devis, G., Vaughan, G. D., Malaisse-Lagae, F., Orci, L., ...
In order to assess the participation of the microfilamentous cell web in the multiphasic response of the pancreatic beta cell, the effect of cytochalasin B upon both glucose- and sulfonylurea-induced...
Orci, L, Montesano, R, Meda, P, Malaisse-Lagae, F, Brown, D, Perrelet, A, ...
The Golgi apparatus is a key element in the ordered movement of secretory polypeptides from the rough endoplasmic reticulum to the plasma membrane during secretion. It has been shown that cisternae...
Determinants of the selective toxicity of alloxan to the pancreatic B cell.
Malaisse, W J, Malaisse-Lagae, F, Sener, A, Pipeleers, D G
The diabetogenic agent alloxan exerts a preferential cytotoxic effect on the pancreatic B cell. The determinants of such a tissue specificity were investigated. Alloxan accumulated rapidly in liver...
Stimulation of pancreatic islet metabolism and insulin release by a nonmetabolizable amino acid.
Sener, A, Malaisse-Lagae, F, Malaisse, W J
The leucine analog beta-2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) activates glutamate dehydrogenase [L-glutamate:NAD+ oxidoreductase (deaminating), EC 1.4.1.2] in pancreatic islet...
Malaisse-Lagae, F, Amherdt, M, Ravazzola, M, Sener, A, Hutton, J C, Orci, L, ...
In the pancreatic B cell, microtubules are thought to be involved in the process of insulin release. Their possible participation in the sequence of events leading from the biosynthesis and...
Glucagon release from rat pancreatic islets. A combined morphological and functional approach.
Carpentier, J L, Malaisse-Lagae, F, Müller, W A
The release of glucagon induced in isolated rat islets by arginine or by calcium deprivation has been subjected to combined functional and morphological quantifications. Arginine-stimulated glucagon...
Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes.
Orci, L, Baetens, D, Rufener, C, Amherdt, M, Ravazzola, M, Studer, P, ...
Insulin-, glucagon-, and somatostatin-contianing cells, identified by immunofluorescent staining, were quantitated morphometrically in sections of pancreas obtained from diabetic and nondiabetic...
Kayser, F, Biesemans, M, Willem, R, Malaisse-Lagae, F, Malaisse, W J
The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the...
Willem, R, Malaisse-Lagae, F, Ottinger, R, Malaisse, W J
The fate of D-[2-13C]glucose 6-phosphate exposed to phosphoglucoisomerase (glucose-6-phosphate isomerase, EC 5.3.1.9) in 2H2O was monitored by 13C-n.m.r. spectroscopy. The generation of the anomers...
Metabolism of endogenous nutrients in islets of Goto-Kakizaki (GK) rats.
Sener, A, Malaisse-Lagae, F, Ostenson, C G, Malaisse, W J
The metabolism of endogenous nutrients was examined in pancreatic islets of control and Goto-Kakizaki (GK) rats. At the ultrastructural level, no glycogen was found in the islet cells of GK rats, a...
Sener, A, Van Schaftingen, E, Van De Winkel, M, Pipeleers, D G, Malaisse-Lagae, F, Malaisse, W J, ...
Glucose caused a sustained and dose-related increase in the fructose 2,6-bisphosphate content of isolated pancreatic islets, as well as of purified pancreatic B-cells. With isolated B-cells, the...
Participation of endogenous fatty acids in the secretory activity of the pancreatic B-cell.
Malaisse, W J, Malaisse-Lagae, F, Sener, A, Hellerström, C
The pancreatic B-cell may represent a fuel-sensor organ, the release of insulin evoked by nutrient secretagogues being attributable to an increased oxidation of exogenous and/or endogenous...
The coupling of metabolic to secretory events in pancreatic islets. The cytosolic redox state.
Sener, A, Malaisse-Lagae, F, Dufrane, S P, Malaisse, W J
NADP-linked isocitrate dehydrogenase and malic enzyme [malate dehydrogenase (decarboxylating) (NADP)] activities were characterized in the cytosol of pancreatic islet cells. D-Glucose and L-leucine...
Mechanism of 3-phenylpyruvate-induced insulin release. Secretory, ionic and oxidative aspects.
Sener, A, Welsh, M, Lebrun, P, Garcia-Morales, P, Saceda, M, Malaisse-Lagae, F, ...
1. 3-Phenylpyruvate caused a dose-related stimulation of insulin release from rat pancreatic islets deprived of exogenous nutrient or incubated in the presence of 5.6 or 8.3 mM-D-glucose. 2....
Mechanism of 3-phenylpyruvate-induced insulin release. Metabolic aspects.
Malaisse, W J, Sener, A, Welsh, M, Malaisse-Lagae, F, Hellerström, C, Christophe, J
1. The metabolism and metabolic effects of 3-phenylpyruvate were examined in rat pancreatic islets. 2. Islet homogenates catalysed transamination reactions between 3-phenylpyruvate and L-glutamate,...
Sener, A, Malaisse-Lagae, F, Malaisse, W J
L-Glutamine at a near-physiological concentration (1.0mM) was rapidly taken up and metabolized in rat pancreatic islets. The rate of glutamine deamidation much exceeded that of glutamate conversion...
Malaisse, W J, Sener, A, Koser, M, Ravazzola, M, Malaisse-Lagae, F
1. When pancreatic islets are preincubated for 20h in the presence of glucose (83.3mM) and thereafter transferred to a glucose-free medium, theophylline (1.4mM) provokes a dramatic stimulation of...
A Possible Role for the Adenylcyclase System in Insulin Secretion*
Malaisse, W. J., Malaisse-Lagae, F., Mayhew, D.
A possible role for adenylcyclase in insulin secretion was investigated. Isoproterenol, a predominantly β-adrenergic agent, when mixed with an α-adrenergic blocking agent (phenoxybenzamine),...
Orci, L, Malaisse-Lagae, F, Ravazzola, M, Rouiller, D, Renold, A E, Perrelet, A, ...
By degranulating beta-cells in the islets of Langerhans of the rat with sulfonylurea, it has been possible to distinguish unambiguously alpha-cells from beta-cells in freeze-fracture replicas. In...
Dynamics of Insulin Release and Microtubular-Microfilamentous System. I. EFFECT OF CYTOCHALASIN B
Van Obberghen, E., Somers, G., Devis, G., Vaughan, G. D., Malaisse-Lagae, F., Orci, L., ...
In order to assess the participation of the microfilamentous cell web in the multiphasic response of the pancreatic beta cell, the effect of cytochalasin B upon both glucose- and sulfonylurea-induced...
Orci, L, Montesano, R, Meda, P, Malaisse-Lagae, F, Brown, D, Perrelet, A, ...
The Golgi apparatus is a key element in the ordered movement of secretory polypeptides from the rough endoplasmic reticulum to the plasma membrane during secretion. It has been shown that cisternae...
Determinants of the selective toxicity of alloxan to the pancreatic B cell.
Malaisse, W J, Malaisse-Lagae, F, Sener, A, Pipeleers, D G
The diabetogenic agent alloxan exerts a preferential cytotoxic effect on the pancreatic B cell. The determinants of such a tissue specificity were investigated. Alloxan accumulated rapidly in liver...
Stimulation of pancreatic islet metabolism and insulin release by a nonmetabolizable amino acid.
Sener, A, Malaisse-Lagae, F, Malaisse, W J
The leucine analog beta-2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) activates glutamate dehydrogenase [L-glutamate:NAD+ oxidoreductase (deaminating), EC 1.4.1.2] in pancreatic islet...
Malaisse-Lagae, F, Amherdt, M, Ravazzola, M, Sener, A, Hutton, J C, Orci, L, ...
In the pancreatic B cell, microtubules are thought to be involved in the process of insulin release. Their possible participation in the sequence of events leading from the biosynthesis and...
Glucagon release from rat pancreatic islets. A combined morphological and functional approach.
Carpentier, J L, Malaisse-Lagae, F, Müller, W A
The release of glucagon induced in isolated rat islets by arginine or by calcium deprivation has been subjected to combined functional and morphological quantifications. Arginine-stimulated glucagon...
Hypertrophy and hyperplasia of somatostatin-containing D-cells in diabetes.
Orci, L, Baetens, D, Rufener, C, Amherdt, M, Ravazzola, M, Studer, P, ...
Insulin-, glucagon-, and somatostatin-contianing cells, identified by immunofluorescent staining, were quantitated morphometrically in sections of pancreas obtained from diabetic and nondiabetic...
Kayser, F, Biesemans, M, Willem, R, Malaisse-Lagae, F, Malaisse, W J
The study by two-dimensional phase-sensitive 31P exchange spectroscopy (EXSY) n.m.r. of hexose 6-phosphates interconversion in the reaction catalysed by yeast phosphoglucoisomerase reveals that the...
Willem, R, Malaisse-Lagae, F, Ottinger, R, Malaisse, W J
The fate of D-[2-13C]glucose 6-phosphate exposed to phosphoglucoisomerase (glucose-6-phosphate isomerase, EC 5.3.1.9) in 2H2O was monitored by 13C-n.m.r. spectroscopy. The generation of the anomers...
Metabolism of endogenous nutrients in islets of Goto-Kakizaki (GK) rats.
Sener, A, Malaisse-Lagae, F, Ostenson, C G, Malaisse, W J
The metabolism of endogenous nutrients was examined in pancreatic islets of control and Goto-Kakizaki (GK) rats. At the ultrastructural level, no glycogen was found in the islet cells of GK rats, a...
Sener, A, Van Schaftingen, E, Van De Winkel, M, Pipeleers, D G, Malaisse-Lagae, F, Malaisse, W J, ...
Glucose caused a sustained and dose-related increase in the fructose 2,6-bisphosphate content of isolated pancreatic islets, as well as of purified pancreatic B-cells. With isolated B-cells, the...
Participation of endogenous fatty acids in the secretory activity of the pancreatic B-cell.
Malaisse, W J, Malaisse-Lagae, F, Sener, A, Hellerström, C
The pancreatic B-cell may represent a fuel-sensor organ, the release of insulin evoked by nutrient secretagogues being attributable to an increased oxidation of exogenous and/or endogenous...
The coupling of metabolic to secretory events in pancreatic islets. The cytosolic redox state.
Sener, A, Malaisse-Lagae, F, Dufrane, S P, Malaisse, W J
NADP-linked isocitrate dehydrogenase and malic enzyme [malate dehydrogenase (decarboxylating) (NADP)] activities were characterized in the cytosol of pancreatic islet cells. D-Glucose and L-leucine...
Mechanism of 3-phenylpyruvate-induced insulin release. Secretory, ionic and oxidative aspects.
Sener, A, Welsh, M, Lebrun, P, Garcia-Morales, P, Saceda, M, Malaisse-Lagae, F, ...
1. 3-Phenylpyruvate caused a dose-related stimulation of insulin release from rat pancreatic islets deprived of exogenous nutrient or incubated in the presence of 5.6 or 8.3 mM-D-glucose. 2....
Mechanism of 3-phenylpyruvate-induced insulin release. Metabolic aspects.
Malaisse, W J, Sener, A, Welsh, M, Malaisse-Lagae, F, Hellerström, C, Christophe, J
1. The metabolism and metabolic effects of 3-phenylpyruvate were examined in rat pancreatic islets. 2. Islet homogenates catalysed transamination reactions between 3-phenylpyruvate and L-glutamate,...
Sener, A, Malaisse-Lagae, F, Malaisse, W J
L-Glutamine at a near-physiological concentration (1.0mM) was rapidly taken up and metabolized in rat pancreatic islets. The rate of glutamine deamidation much exceeded that of glutamate conversion...
Malaisse, W J, Sener, A, Koser, M, Ravazzola, M, Malaisse-Lagae, F
1. When pancreatic islets are preincubated for 20h in the presence of glucose (83.3mM) and thereafter transferred to a glucose-free medium, theophylline (1.4mM) provokes a dramatic stimulation of...