Weichsel, A, Montfort, W R, Cieśla, J, Maley, F
A folate analogue, 1843U89 (U89), with potential as a chemotherapeutic agent due to its potent and specific inhibition of thymidylate synthase (TS; EC 2.1.1.45), greatly enhances not only the binding...
Identification of an RNA binding site for human thymidylate synthase.
Chu, E, Voeller, D, Koeller, D M, Drake, J C, Takimoto, C H, Maley, G F, ...
Previous studies from this laboratory have shown that human TS mRNA translation is regulated by its protein product in a negative autoregulatory manner. In this paper, we identify an RNA binding site...
Characterization of the restriction site of a prokaryotic intron-encoded endonuclease.
Chu, F K, Maley, G, Pedersen-Lane, J, Wang, A M, Maley, F
The 1016-base-pair (bp) intron in the T4 bacteriophage thymidylate synthase gene (td) contains a 735-bp open reading frame that encodes a protein product with endonucleolytic activity. The...
Chen, X, Mathews, C K, Wheeler, L J, Maley, G, Maley, F, Coombs, D H
Numerous reports describe the phage T4 enzymes thymidylate synthase and dihydrofolate reductase as structural components of the baseplate. However, Y. Wang and C. K. Mathews (J. Virol. 63:4736-4743,...
Chu, F K, Maley, F, Martinez, J, Maley, G F
Southern hybridization analyses of procaryotic DNA from Escherichia coli, lambda bacteriophage, and T1 to T7 phages were carried out. The hybridization probes used consisted of DNA restriction...
Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.
Chu, E, Takechi, T, Jones, K L, Voeller, D M, Copur, S M, Maley, G F, ...
Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS...
Voeller, D M, Changchien, L M, Maley, G F, Maley, F, Takechi, T, Turner, R E, ...
Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct...
Purification and substrate specificity of a T4 phage intron-encoded endonuclease.
Chu, F K, Maley, F, Wang, A M, Pedersen-Lane, J, Maley, G
The T4 phage td intron-encoded endonuclease (I-Tev I) cleaves the intron-deleted td gene (td delta I) 23 nucleotides upstream of the intron insertion site on the noncoding strand and 25 nucleotides...
Intervening sequence in the thymidylate synthase gene of bacteriophage T4.
Chu, F K, Maley, G F, Maley, F, Belfort, M
The continuous sequence of 2.3 kilobases in a 3-kilobase DNA fragment encoding the structural gene for coliphage T4 thymidylate synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC...
Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
Chu, E, Voeller, D M, Jones, K L, Takechi, T, Maley, G F, Maley, F, ...
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS...
Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Belfort, M, Maley, G, Pedersen-Lane, J, Maley, F
The nucleotide sequence of a 1,163-base-pair fragment that encodes the entire thyA gene of Escherichia coli K-12 was determined. The strategy involved sequence determination of both DNA strands by...
Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway.
Ehrenman, K, Pedersen-Lane, J, West, D, Herman, R, Maley, F, Belfort, M
Several features of the split td gene of phage T4 suggest an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work...
Characterization of the Escherichia coli thyA gene and its amplified thymidylate synthetase product.
Belfort, M, Maley, G F, Maley, F
The 7.8-kilobase HindIII insert in phage lambda NM589thyA [Borck, K., Beggs, J.D., Brammar, W.J., Hopkins, A.S. & Murray, N. (1976) Mol. Gen. Genet. 146, 199] was confirmed as originating from...
Amino acid sequence at the FdUMP binding site of thymidylate synthetase.
Bellisario, R L, Maley, G F, Galivan, J H, Maley, F
Cyanogen bromide treatment of thymidylate synthetase of Lactobacillus casei, which had been converted to a ternary complex with [2-14c] FdUMP and 5,10-methylene-tetrahydrofolate followed by...
Weichsel, A, Montfort, W R, Cieśla, J, Maley, F
A folate analogue, 1843U89 (U89), with potential as a chemotherapeutic agent due to its potent and specific inhibition of thymidylate synthase (TS; EC 2.1.1.45), greatly enhances not only the binding...
Identification of an RNA binding site for human thymidylate synthase.
Chu, E, Voeller, D, Koeller, D M, Drake, J C, Takimoto, C H, Maley, G F, ...
Previous studies from this laboratory have shown that human TS mRNA translation is regulated by its protein product in a negative autoregulatory manner. In this paper, we identify an RNA binding site...
Characterization of the restriction site of a prokaryotic intron-encoded endonuclease.
Chu, F K, Maley, G, Pedersen-Lane, J, Wang, A M, Maley, F
The 1016-base-pair (bp) intron in the T4 bacteriophage thymidylate synthase gene (td) contains a 735-bp open reading frame that encodes a protein product with endonucleolytic activity. The...
Chen, X, Mathews, C K, Wheeler, L J, Maley, G, Maley, F, Coombs, D H
Numerous reports describe the phage T4 enzymes thymidylate synthase and dihydrofolate reductase as structural components of the baseplate. However, Y. Wang and C. K. Mathews (J. Virol. 63:4736-4743,...
Chu, F K, Maley, F, Martinez, J, Maley, G F
Southern hybridization analyses of procaryotic DNA from Escherichia coli, lambda bacteriophage, and T1 to T7 phages were carried out. The hybridization probes used consisted of DNA restriction...
Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.
Chu, E, Takechi, T, Jones, K L, Voeller, D M, Copur, S M, Maley, G F, ...
Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS...
Voeller, D M, Changchien, L M, Maley, G F, Maley, F, Takechi, T, Turner, R E, ...
Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct...
Purification and substrate specificity of a T4 phage intron-encoded endonuclease.
Chu, F K, Maley, F, Wang, A M, Pedersen-Lane, J, Maley, G
The T4 phage td intron-encoded endonuclease (I-Tev I) cleaves the intron-deleted td gene (td delta I) 23 nucleotides upstream of the intron insertion site on the noncoding strand and 25 nucleotides...
Intervening sequence in the thymidylate synthase gene of bacteriophage T4.
Chu, F K, Maley, G F, Maley, F, Belfort, M
The continuous sequence of 2.3 kilobases in a 3-kilobase DNA fragment encoding the structural gene for coliphage T4 thymidylate synthase (5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC...
Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
Chu, E, Voeller, D M, Jones, K L, Takechi, T, Maley, G F, Maley, F, ...
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS...
Primary structure of the Escherichia coli thyA gene and its thymidylate synthase product.
Belfort, M, Maley, G, Pedersen-Lane, J, Maley, F
The nucleotide sequence of a 1,163-base-pair fragment that encodes the entire thyA gene of Escherichia coli K-12 was determined. The strategy involved sequence determination of both DNA strands by...
Processing of phage T4 td-encoded RNA is analogous to the eukaryotic group I splicing pathway.
Ehrenman, K, Pedersen-Lane, J, West, D, Herman, R, Maley, F, Belfort, M
Several features of the split td gene of phage T4 suggest an RNA processing mechanism analogous to that of the self-splicing rRNA of Tetrahymena and other group I eukaryotic introns. Previous work...
Characterization of the Escherichia coli thyA gene and its amplified thymidylate synthetase product.
Belfort, M, Maley, G F, Maley, F
The 7.8-kilobase HindIII insert in phage lambda NM589thyA [Borck, K., Beggs, J.D., Brammar, W.J., Hopkins, A.S. & Murray, N. (1976) Mol. Gen. Genet. 146, 199] was confirmed as originating from...
Amino acid sequence at the FdUMP binding site of thymidylate synthetase.
Bellisario, R L, Maley, G F, Galivan, J H, Maley, F
Cyanogen bromide treatment of thymidylate synthetase of Lactobacillus casei, which had been converted to a ternary complex with [2-14c] FdUMP and 5,10-methylene-tetrahydrofolate followed by...
Crystal structures of a marginally active thymidylate synthase mutant, Arg 126-->Glu.
Strop, P., Changchien, L., Maley, F., Montfort, W. R.
Thymidylate synthase (TS) is a long-standing target for anticancer drugs and is of interest for its rich mechanistic features. The enzyme catalyzes the conversion of dUMP to dTMP using the co-enzyme...
Crystal structure of thymidylate synthase A from Bacillus subtilis.
Fox, K. M., Maley, F., Garibian, A., Changchien, L. M., Van Roey, P.
Thymidylate synthase (TS) converts dUMP to dTMP by reductive methylation, where 5,10-methylenetetrahydrofolate is the source of both the methylene group and reducing equivalents. The mechanism of...