Kallenbach, S, Doyen, N, Fanton D'Andon, M, Rougeon, F
The somatic diversity immunoglobulin and T-cell receptor diversity is largely provided by the junctional variation created during site-specific rearrangement of separately encoded gene segments....
Rabilloud, T, Pennetier, J L, Hibner, U, Vincens, P, Tarroux, P, Rougeon, F
Total nuclear proteins extracted from cell lines representing various stages of differentiation of mouse B lymphocytes were studied by computer analysis of two-dimensional gels. Of the 1438 spots...
Delarue, M., Boulé, J.B., Lescar, J., Expert-Bezançon, N., Jourdan, N., Sukumar, N., ...
The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 Å resolution reveals a typical DNA polymerase β-like fold locked in a closed form. In...
The presence of direct repeats does not influence coding joint formation during V(D)J recombination.
Nourrit, F, Nguyen, Q T, Rougeon, F, Kallenbach, S
During the recombination process that assembles immunoglobulin and T-cell receptor gene segments, the coding ends to be joined are extensively processed. Contradictory reports have been made in the...
Rosinski-Chupin, I, Tronik, D, Rougeon, F
NaDodSO4/PAGE analysis of in vitro translation products of rat submaxillary gland (SMG) mRNAs has revealed an important sexual dimorphism. Moreover, most of the rat male-specific major translation...
Extra-renal transcription of the renin genes in multiple tissues of mice and rats.
Ekker, M, Tronik, D, Rougeon, F
Expression of the mouse renin genes (Ren-1 and Ren-2) and of the unique rat renin gene was determined in several extra-renal tissues of mice and rats by primer-directed enzymatic amplification of...
Rearrangement and expression of rabbit immunoglobulin kappa light chain gene in transgenic mice.
Goodhardt, M, Cavelier, P, Akimenko, M A, Lutfalla, G, Babinet, C, Rougeon, F
To determine whether a foreign unrearranged immunoglobulin gene can be functionally rearranged and expressed in vivo, a rabbit b9 kappa light chain gene construct containing a single germ-line kappa...
Mariame, B, Akimenko, M A, Rougeon, F
Contrary to the situation in humans or mice, where the constant region (C) of the Immunoglobulin (Ig) kappa (kappa) light chain is encoded by a single gene, the rabbit possesses two C kappa genes: C...
Differential splicing in mouse thymus generates two forms of terminal deoxynucleotidyl transferase.
Doyen, N, D'Andon, M F, Bentolila, L A, Nguyen, Q T, Rougeon, F
A new form of TdT mRNA has been identified by screening a mouse thymus cDNA library. It contains an open reading frame of 1527 base pairs corresponding to a protein containing 509 aminoacids, whereas...
Regulatory elements involved in the bidirectional activity of an immunoglobulin promoter.
Doyen, N, Dreyfus, M, Rougeon, F
We show that the promoter from the mouse VH441 heavy-chain immunoglobulin gene, when present on plasmids transiently introduced into myeloma cells, promotes transcription bidirectionally, due to the...
Akimenko, M A, Ekker, M, Doyen, N, Biben, C, Rougeon, F
Enhancer activity of the rabbit immunoglobulin kappa light chain gene intron conserved region (KICR) was examined in mouse myeloma cells using transient expression experiments. Compared to the...
Complex allotypes of the rabbit immunoglobulin kappa light chains are encoded by structural alleles.
Akimenko, M A, Heidmann, O, Rougeon, F
We have isolated the rabbit immunoglobulin b9 Ck light chain gene and compared its nucleotide sequence with the b4, b4var , b5 and bas Ck sequences. In spite of the high number of substitutions found...
Ollo, R, Auffray, C, Morchamps, C, Rougeon, F
A 23-kilobase EcoRI DNA fragment coding for the BALB/c immunoglobulin gamma 2a chain was cloned from mouse embryo DNA in the cosmid pJC74, and a nucleotide sequence of 1904 bases was determined for...
Molecular cloning of rabbit gamma heavy chain mRNA.
A cDNA library of rabbit spleen mRNA was screened for immunoglobulin heavy chain sequences. In this paper we report the nucleotide sequence of two cDNA clones containing part of the constant region...
Walking along the rabies genome: is the large G-L intergenic region a remnant gene?
Tordo, N, Poch, O, Ermine, A, Keith, G, Rougeon, F
Rabies cDNA clones, obtained by "walking along the genome" using two successive DNA primers, have allowed the sequence determination of the genes encoding the N, M1, M2, G, and the beginning of the L...
Akimenko, M A, Mariamé, B, Rougeon, F
The rabbit kappa light chain gene family is characterized by the presence of two constant region (C kappa) genes; the C kappa 1 gene encodes the constant region of the principal rabbit immunoglobulin...
Auffray, C, Nageotte, R, Chambraud, B, Rougeon, F
A DNA sequence complementary to the entire coding part of a mouse gamma 2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the...
Sikorav, J L, Auffray, C, Rougeon, F
The complete sequence for the constant and 3' untranslated regions of a mouse gamma 2a immunoglobulin heavy chain mRNA is reported. The sequence is 1093 nucleotides long coding for the CH1...
Molecular cloning and nucleotide sequence of a human renin cDNA fragment.
Soubrier, F, Panthier, J J, Corvol, P, Rougeon, F
We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) renin cDNA probe. The human kidney messenger RNA is about 1.6 kilobase (kb) long, similarly to the...
Structural relationships among mouse and human immunoglobulin VH genes in the subgroup III.
Ollo, R, Sikorav, J L, Rougeon, F
The mouse VHIII subgroup is composed of four families which share sequence homology. We isolated a VH germ-line genomic clone, which cross hybridizes with a cDNA probe from one of these families,...
Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
Ollo, R, Auffray, C, Sikorav, J L, Rougeon, F
We have constructed a library of Balb/c mouse embryo DNA in the vector Charon 4A. The library was searched for sequences homologous to the VH region of a cloned cDNA of the UPC10 heavy chain mRNA. In...
Molecular cloning and nucleotide sequence of the human growth hormone structural gene.
An almost complete cDNA copy of human growth hormone has been cloned and sequenced. The nucleotide sequence confirms the known protein sequence and predicts the sequence of a precursor region of 26...
Nguyen, Q T, Doyen, N, D'Andon, M F, Rougeon, F
The mouse heavy chain immunoglobulin promoter VH441 can lead in vitro to bidirectional transcription, due to a symmetrical organization of immunoglobulin heavy chain promoters with two TATA-like...
Goodhardt, M, Babinet, C, Lutfalla, G, Kallenbach, S, Cavelier, P, Rougeon, F
We have produced transgenic mice which synthesize chimeric mouse-rabbit immunoglobulin (Ig) kappa light chains following in vivo recombination of an injected unrearranged kappa gene. The exogenous...
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
Rougeon, F, Kourilsky, P, Mach, B
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired...
Heidmann, O, Auffray, C, Cazenave, P A, Rougeon, F
A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated from the spleen and translated in vitro, and translation products...
Molecular cloning of a mouse submaxillary gland renin cDNA fragment.
Rougeon, F, Chambraud, B, Foote, S, Panthier, J J, Nageotte, R, Corvol, P
The mRNA encoding mouse renin has been partially purified from total poly(A)-containing RNA of submaxillary glands of male Swiss mice. Corresponding cDNAs were cloned in the Pst I site of pBR322....
Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences.
Panthier, J J, Dreyfus, M, Roux, T L, Rougeon, F
The genomic DNAs that specify the renin mRNAs found in the submaxillary gland and in the kidney of Swiss mice have been isolated by molecular cloning in phage lambda. The Ren1 and Ren2 genes,...
Bentolila, L A, Fanton D'Andon, M, Nguyen, Q T, Martinez, O, Rougeon, F, Doyen, N
Two alternatively spliced terminal deoxynucleotidyl transferase transcripts, TdTS and TdTL which code respectively for proteins of 509 and 529 amino acids have been previously identified in the mouse...
Two approaches have been explored for the synthesis of double-stranded DNA from single-stranded DNA template complementary to rabbit 9S globin mRNA (cDNA). (i) cDNA was elongated with dCMP or dTMP...
Panthier, J J, Holm, I, Rougeon, F
Inbred strains of mice have been divided into two distinct phenotypic groups having different levels of renin activity regulated by androgen in the submaxillary gland (SMG). Strains carrying the Rnrs...
Giraudat, J, Devillers-Thiery, A, Auffray, C, Rougeon, F, Changeux, J P
A recombinant DNA plasmid has been constructed that contains sequences of the gene coding for the acetylcholine binding subunit (alpha-subunit, 40 000 daltons) of Torpedo marmorata acetylcholine...
Tronik, D, Dreyfus, M, Babinet, C, Rougeon, F
The Ren-2 gene encoding the mouse submaxillary gland (SMG) renin was microinjected into the pronuclei of fertilized eggs from mice carrying only the Ren-1 gene. In addition to the whole transcription...
Dreyfus, M, Doyen, N, Rougeon, F
A conserved decanucleotide (ATGCAAATNA) is present 45-60 nucleotides upstream from the transcription startpoint in all immunoglobulin heavy chain promoters (VH promoters). We have introduced this...
We have constructed a genomic library of homozygous b4b4 rabbit DNA in the pJB8 cosmid vector. Clones containing Ckappa-like sequences were screened with a b4 cDNA probe and were characterized by...
Kidney and submaxillary gland renins are encoded by two non-allelic genes in Swiss mice.
Two distinct phenotypic groups of inbred strains of mice, with different amounts of submaxillary gland (SMG) renin have been described. We have previously shown that strains with high levels of SMG...
Holm, I, Ollo, R, Panthier, J J, Rougeon, F
Overlapping recombinant clones that appear to encompass the entire renin gene, named Ren 1, have been isolated from a library of BALB/c mouse genomic DNA fragments. Based on restriction endonuclease...
Kallenbach, S, Doyen, N, Fanton D'Andon, M, Rougeon, F
The somatic diversity immunoglobulin and T-cell receptor diversity is largely provided by the junctional variation created during site-specific rearrangement of separately encoded gene segments....
Rabilloud, T, Pennetier, J L, Hibner, U, Vincens, P, Tarroux, P, Rougeon, F
Total nuclear proteins extracted from cell lines representing various stages of differentiation of mouse B lymphocytes were studied by computer analysis of two-dimensional gels. Of the 1438 spots...
Delarue, M., Boulé, J.B., Lescar, J., Expert-Bezançon, N., Jourdan, N., Sukumar, N., ...
The crystal structure of the catalytic core of murine terminal deoxynucleotidyltransferase (TdT) at 2.35 Å resolution reveals a typical DNA polymerase β-like fold locked in a closed form. In...
The presence of direct repeats does not influence coding joint formation during V(D)J recombination.
Nourrit, F, Nguyen, Q T, Rougeon, F, Kallenbach, S
During the recombination process that assembles immunoglobulin and T-cell receptor gene segments, the coding ends to be joined are extensively processed. Contradictory reports have been made in the...
Rosinski-Chupin, I, Tronik, D, Rougeon, F
NaDodSO4/PAGE analysis of in vitro translation products of rat submaxillary gland (SMG) mRNAs has revealed an important sexual dimorphism. Moreover, most of the rat male-specific major translation...
Extra-renal transcription of the renin genes in multiple tissues of mice and rats.
Ekker, M, Tronik, D, Rougeon, F
Expression of the mouse renin genes (Ren-1 and Ren-2) and of the unique rat renin gene was determined in several extra-renal tissues of mice and rats by primer-directed enzymatic amplification of...
Rearrangement and expression of rabbit immunoglobulin kappa light chain gene in transgenic mice.
Goodhardt, M, Cavelier, P, Akimenko, M A, Lutfalla, G, Babinet, C, Rougeon, F
To determine whether a foreign unrearranged immunoglobulin gene can be functionally rearranged and expressed in vivo, a rabbit b9 kappa light chain gene construct containing a single germ-line kappa...
Mariame, B, Akimenko, M A, Rougeon, F
Contrary to the situation in humans or mice, where the constant region (C) of the Immunoglobulin (Ig) kappa (kappa) light chain is encoded by a single gene, the rabbit possesses two C kappa genes: C...
Differential splicing in mouse thymus generates two forms of terminal deoxynucleotidyl transferase.
Doyen, N, D'Andon, M F, Bentolila, L A, Nguyen, Q T, Rougeon, F
A new form of TdT mRNA has been identified by screening a mouse thymus cDNA library. It contains an open reading frame of 1527 base pairs corresponding to a protein containing 509 aminoacids, whereas...
Regulatory elements involved in the bidirectional activity of an immunoglobulin promoter.
Doyen, N, Dreyfus, M, Rougeon, F
We show that the promoter from the mouse VH441 heavy-chain immunoglobulin gene, when present on plasmids transiently introduced into myeloma cells, promotes transcription bidirectionally, due to the...
Akimenko, M A, Ekker, M, Doyen, N, Biben, C, Rougeon, F
Enhancer activity of the rabbit immunoglobulin kappa light chain gene intron conserved region (KICR) was examined in mouse myeloma cells using transient expression experiments. Compared to the...
Complex allotypes of the rabbit immunoglobulin kappa light chains are encoded by structural alleles.
Akimenko, M A, Heidmann, O, Rougeon, F
We have isolated the rabbit immunoglobulin b9 Ck light chain gene and compared its nucleotide sequence with the b4, b4var , b5 and bas Ck sequences. In spite of the high number of substitutions found...
Ollo, R, Auffray, C, Morchamps, C, Rougeon, F
A 23-kilobase EcoRI DNA fragment coding for the BALB/c immunoglobulin gamma 2a chain was cloned from mouse embryo DNA in the cosmid pJC74, and a nucleotide sequence of 1904 bases was determined for...
Molecular cloning of rabbit gamma heavy chain mRNA.
A cDNA library of rabbit spleen mRNA was screened for immunoglobulin heavy chain sequences. In this paper we report the nucleotide sequence of two cDNA clones containing part of the constant region...
Walking along the rabies genome: is the large G-L intergenic region a remnant gene?
Tordo, N, Poch, O, Ermine, A, Keith, G, Rougeon, F
Rabies cDNA clones, obtained by "walking along the genome" using two successive DNA primers, have allowed the sequence determination of the genes encoding the N, M1, M2, G, and the beginning of the L...
Akimenko, M A, Mariamé, B, Rougeon, F
The rabbit kappa light chain gene family is characterized by the presence of two constant region (C kappa) genes; the C kappa 1 gene encodes the constant region of the principal rabbit immunoglobulin...
Auffray, C, Nageotte, R, Chambraud, B, Rougeon, F
A DNA sequence complementary to the entire coding part of a mouse gamma 2a immunoglobulin heavy chain mRNA isolated from a myeloma producing a levan binding protein (UPC 10), has been cloned in the...
Sikorav, J L, Auffray, C, Rougeon, F
The complete sequence for the constant and 3' untranslated regions of a mouse gamma 2a immunoglobulin heavy chain mRNA is reported. The sequence is 1093 nucleotides long coding for the CH1...
Molecular cloning and nucleotide sequence of a human renin cDNA fragment.
Soubrier, F, Panthier, J J, Corvol, P, Rougeon, F
We have studied human renin messenger RNA by hybridization with the mouse submaxillary gland (SMG) renin cDNA probe. The human kidney messenger RNA is about 1.6 kilobase (kb) long, similarly to the...
Structural relationships among mouse and human immunoglobulin VH genes in the subgroup III.
Ollo, R, Sikorav, J L, Rougeon, F
The mouse VHIII subgroup is composed of four families which share sequence homology. We isolated a VH germ-line genomic clone, which cross hybridizes with a cDNA probe from one of these families,...
Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
Ollo, R, Auffray, C, Sikorav, J L, Rougeon, F
We have constructed a library of Balb/c mouse embryo DNA in the vector Charon 4A. The library was searched for sequences homologous to the VH region of a cloned cDNA of the UPC10 heavy chain mRNA. In...
Molecular cloning and nucleotide sequence of the human growth hormone structural gene.
An almost complete cDNA copy of human growth hormone has been cloned and sequenced. The nucleotide sequence confirms the known protein sequence and predicts the sequence of a precursor region of 26...
Nguyen, Q T, Doyen, N, D'Andon, M F, Rougeon, F
The mouse heavy chain immunoglobulin promoter VH441 can lead in vitro to bidirectional transcription, due to a symmetrical organization of immunoglobulin heavy chain promoters with two TATA-like...
Goodhardt, M, Babinet, C, Lutfalla, G, Kallenbach, S, Cavelier, P, Rougeon, F
We have produced transgenic mice which synthesize chimeric mouse-rabbit immunoglobulin (Ig) kappa light chains following in vivo recombination of an injected unrearranged kappa gene. The exogenous...
Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
Rougeon, F, Kourilsky, P, Mach, B
Double stranded DNA has been synthesized in vitro from rabbit globin messenger RNA and elongated with homopolymeric dG tails. An E. coli plasmid was cleaved by EcoRI. The cohesive ends were repaired...
Heidmann, O, Auffray, C, Cazenave, P A, Rougeon, F
A homozygous a2/a2 and b4/b4 rabbit has been hyperimmunized with Micrococcus lysodeikticus. Poly(A)-containing RNA has been isolated from the spleen and translated in vitro, and translation products...
Molecular cloning of a mouse submaxillary gland renin cDNA fragment.
Rougeon, F, Chambraud, B, Foote, S, Panthier, J J, Nageotte, R, Corvol, P
The mRNA encoding mouse renin has been partially purified from total poly(A)-containing RNA of submaxillary glands of male Swiss mice. Corresponding cDNAs were cloned in the Pst I site of pBR322....
Mouse kidney and submaxillary gland renin genes differ in their 5' putative regulatory sequences.
Panthier, J J, Dreyfus, M, Roux, T L, Rougeon, F
The genomic DNAs that specify the renin mRNAs found in the submaxillary gland and in the kidney of Swiss mice have been isolated by molecular cloning in phage lambda. The Ren1 and Ren2 genes,...
Bentolila, L A, Fanton D'Andon, M, Nguyen, Q T, Martinez, O, Rougeon, F, Doyen, N
Two alternatively spliced terminal deoxynucleotidyl transferase transcripts, TdTS and TdTL which code respectively for proteins of 509 and 529 amino acids have been previously identified in the mouse...
Two approaches have been explored for the synthesis of double-stranded DNA from single-stranded DNA template complementary to rabbit 9S globin mRNA (cDNA). (i) cDNA was elongated with dCMP or dTMP...
Panthier, J J, Holm, I, Rougeon, F
Inbred strains of mice have been divided into two distinct phenotypic groups having different levels of renin activity regulated by androgen in the submaxillary gland (SMG). Strains carrying the Rnrs...
Giraudat, J, Devillers-Thiery, A, Auffray, C, Rougeon, F, Changeux, J P
A recombinant DNA plasmid has been constructed that contains sequences of the gene coding for the acetylcholine binding subunit (alpha-subunit, 40 000 daltons) of Torpedo marmorata acetylcholine...
Tronik, D, Dreyfus, M, Babinet, C, Rougeon, F
The Ren-2 gene encoding the mouse submaxillary gland (SMG) renin was microinjected into the pronuclei of fertilized eggs from mice carrying only the Ren-1 gene. In addition to the whole transcription...
Dreyfus, M, Doyen, N, Rougeon, F
A conserved decanucleotide (ATGCAAATNA) is present 45-60 nucleotides upstream from the transcription startpoint in all immunoglobulin heavy chain promoters (VH promoters). We have introduced this...