Monteseirín, J., Chacón, P., Vega, A., Camacho, M.J., Bonilla, I., Guardia, P., ...
Aunque desde hace años se ha implicado al eosinófilo en la fisiopatología de los procesos alérgicos mediados por la IgE, todavía no se conocen los mecanismos exactos que atraen a estas células...
Monteseirín, J., Chacón, P., Vega, A., Camacho, M.J., Bonilla, I., Guardia, P., ...
Aunque desde hace años se ha implicado al eosinófilo en la fisiopatología de los procesos alérgicos mediados por la IgE, todavía no se conocen los mecanismos exactos que atraen a estas células...
IRES-driven translation is stimulated separately by the FMDV 3'-NCR and poly(A) sequences. (2002)
Lopez De Quinto, Sonia, Sáiz, Margarita, Martínez-Salas, E., Sobrino, F.
IRES-driven translation is stimulated separately by the FMDV 3'-NCR and poly(A) sequences. (2002)
Lopez De Quinto, Sonia, Sáiz, Margarita, Martínez-Salas, E., Sobrino, F.
Design of primers for PCR ampiification of highly variable genomes (1993)
Dopazo, J., Rodriguez, A., Sáiz, J.C., Sobrino, F.
A program to aid in the search of primers for specific polymerase chain reaction (PCR) amplification of highly variable genomes is presented. It involves the derivation of variability profiles to...
Taboga, O, Tami, C, Carrillo, E, Núñez, J I, Rodríguez, A, Saíz, J C, ...
A large-scale vaccination experiment involving a total of 138 cattle was carried out to evaluate the potential of synthetic peptides as vaccines against foot-and-mouth disease. Four types of peptides...
Kirchweger, R, Ziegler, E, Lamphear, B J, Waters, D, Liebig, H D, Sommergruber, W, ...
Many picornaviruses cause a dramatic decrease in the translation of cellular mRNAs in the infected cell, without affecting the translation of their own RNA. Specific proteolysis of protein synthesis...
Gutiérrez, A, Martínez-Salas, E, Pintado, B, Sobrino, F
RNA molecules containing the 3' terminal region of foot-and-mouth disease virus (FMDV) RNA in both antisense and sense orientations were able to inhibit viral FMDV translation and infective particle...
Saiz, J C, Gonzalez, M J, Borca, M V, Sobrino, F, Moore, D M
Five neutralizing monoclonal antibodies (nMAbs) obtained against type A5 Spain-86 foot-and-mouth disease virus were used to generate a series of neutralization-resistant variants. In vitro and in...
Martínez, M A, Dopazo, J, Hernández, J, Mateu, M G, Sobrino, F, Domingo, E, ...
The genetic diversification of foot-and-mouth disease virus (FMDV) of serotype C over a 6-decade period was studied by comparing nucleotide sequences of the capsid protein-coding regions of viruses...
Martínez, M A, Carrillo, C, González-Candelas, F, Moya, A, Domingo, E, Sobrino, F
We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21...
Dopazo, J, Sobrino, F, Palma, E L, Domingo, E, Moya, A
A phylogenetic tree relating the VP1 gene of 15 isolates of foot-and-mouth disease virus (FMDV) of serotypes A, C, and O has been constructed. The most parsimonious tree shows that FMDV subtypes and...
VPg gene amplification correlates with infective particle formation in foot-and-mouth disease virus.
Falk, M M, Sobrino, F, Beck, E
In order to analyze the function of VPg amplification in aphthoviruses, we have undertaken the first mutational analysis of the repetitive VPg-coding region using an improved foot-and-mouth disease...
Mateu, M G, Martínez, M A, Rocha, E, Andreu, D, Parejo, J, Giralt, E, ...
We provide evidence that the quasispecies nature (extreme genetic heterogeneity) of foot-and-mouth disease virus is relevant to the virus evading an immune response. A monoclonal antibody...
1. Peritoneal macrophages were prepared from control, Escherichia coli-treated and triamcinolone acetonide-treated rats. Control and E. coli-treated rats produced resident and activated macrophages...
Chiara, M D, Bedoya, F, Sobrino, F
Peritoneal resident macrophages from mice are sensitive to inhibition by cyclosporin A (CsA) of phorbol 12-myristate 13-acetate (PMA)-stimulated oxidative burst. Inhibition was assessed in terms of...
Decrease in free-radical production with age in rat peritoneal macrophages.
Alvarez, E, Conde, M, Machado, A, Sobrino, F, Santa Maria, C
The respiratory-burst reaction has been studied in rat peritoneal macrophages of different ages (3, 12 and 24 months) using phorbol 12-myristate 13-acetate (PMA) to stimulate NADPH oxidase....
Incubation of isolated rat hepatocytes from fasted rats with 0-6 mM-glucose caused an increase in [fructose 2,6-bisphosphate] (0.2 to about 5 nmol/g) without net lactate production. A release of 3H2O...
Gualberto, A, Molinero, P, Sobrino, F
Experimental hypothyroidism was induced in rats by the administration of NaClO4. Hearts from normal and hypothyroid rats were homogenized, and the extracts were assayed for phosphofructokinase-1 and...
Sobrino, F, Rider, M H, Gualberto, A, Hue, L
The concentration of fructose 2,6-bisphosphate found in freshly isolated erythrocytes was below the limit of detection (20 pmol/ml of packed cells). However, it increased to about 250 pmol/ml of...
Stimulating role of potassium ions and ouabain on glycogen synthesis in adipose tissue.
Sobrino, F, Ruiz, G, Goberna, R
1. Exposure of fat-pads to increasing concentrations of K+ in the presence of insulin stimulates the incorporation of labelled glucose into glycogen. In the absence of hormone, only a slight...
Carballo, M, Vilaplana, R, Márquez, G, Conde, M, Bedoya, F J, González-Vílchez, F, ...
To determine the nature of the mechanism by which certain derived ruthenium (Ru) complexes induce regression in tumour growth, we have investigated the possibility that this mechanism was associated...
Taboga, O, Tami, C, Carrillo, E, Núñez, J I, Rodríguez, A, Saíz, J C, ...
A large-scale vaccination experiment involving a total of 138 cattle was carried out to evaluate the potential of synthetic peptides as vaccines against foot-and-mouth disease. Four types of peptides...
Kirchweger, R, Ziegler, E, Lamphear, B J, Waters, D, Liebig, H D, Sommergruber, W, ...
Many picornaviruses cause a dramatic decrease in the translation of cellular mRNAs in the infected cell, without affecting the translation of their own RNA. Specific proteolysis of protein synthesis...
Gutiérrez, A, Martínez-Salas, E, Pintado, B, Sobrino, F
RNA molecules containing the 3' terminal region of foot-and-mouth disease virus (FMDV) RNA in both antisense and sense orientations were able to inhibit viral FMDV translation and infective particle...
Saiz, J C, Gonzalez, M J, Borca, M V, Sobrino, F, Moore, D M
Five neutralizing monoclonal antibodies (nMAbs) obtained against type A5 Spain-86 foot-and-mouth disease virus were used to generate a series of neutralization-resistant variants. In vitro and in...
Martínez, M A, Dopazo, J, Hernández, J, Mateu, M G, Sobrino, F, Domingo, E, ...
The genetic diversification of foot-and-mouth disease virus (FMDV) of serotype C over a 6-decade period was studied by comparing nucleotide sequences of the capsid protein-coding regions of viruses...
Martínez, M A, Carrillo, C, González-Candelas, F, Moya, A, Domingo, E, Sobrino, F
We document the rapid alteration of fitness of two foot-and-mouth disease virus (FMDV) mutants resistant to a neutralizing monoclonal antibody. Both mutants showed a selective disadvantage in BHK-21...
Dopazo, J, Sobrino, F, Palma, E L, Domingo, E, Moya, A
A phylogenetic tree relating the VP1 gene of 15 isolates of foot-and-mouth disease virus (FMDV) of serotypes A, C, and O has been constructed. The most parsimonious tree shows that FMDV subtypes and...
VPg gene amplification correlates with infective particle formation in foot-and-mouth disease virus.
Falk, M M, Sobrino, F, Beck, E
In order to analyze the function of VPg amplification in aphthoviruses, we have undertaken the first mutational analysis of the repetitive VPg-coding region using an improved foot-and-mouth disease...
Mateu, M G, Martínez, M A, Rocha, E, Andreu, D, Parejo, J, Giralt, E, ...
We provide evidence that the quasispecies nature (extreme genetic heterogeneity) of foot-and-mouth disease virus is relevant to the virus evading an immune response. A monoclonal antibody...
1. Peritoneal macrophages were prepared from control, Escherichia coli-treated and triamcinolone acetonide-treated rats. Control and E. coli-treated rats produced resident and activated macrophages...
Chiara, M D, Bedoya, F, Sobrino, F
Peritoneal resident macrophages from mice are sensitive to inhibition by cyclosporin A (CsA) of phorbol 12-myristate 13-acetate (PMA)-stimulated oxidative burst. Inhibition was assessed in terms of...
Decrease in free-radical production with age in rat peritoneal macrophages.
Alvarez, E, Conde, M, Machado, A, Sobrino, F, Santa Maria, C
The respiratory-burst reaction has been studied in rat peritoneal macrophages of different ages (3, 12 and 24 months) using phorbol 12-myristate 13-acetate (PMA) to stimulate NADPH oxidase....
Incubation of isolated rat hepatocytes from fasted rats with 0-6 mM-glucose caused an increase in [fructose 2,6-bisphosphate] (0.2 to about 5 nmol/g) without net lactate production. A release of 3H2O...
Gualberto, A, Molinero, P, Sobrino, F
Experimental hypothyroidism was induced in rats by the administration of NaClO4. Hearts from normal and hypothyroid rats were homogenized, and the extracts were assayed for phosphofructokinase-1 and...
Sobrino, F, Rider, M H, Gualberto, A, Hue, L
The concentration of fructose 2,6-bisphosphate found in freshly isolated erythrocytes was below the limit of detection (20 pmol/ml of packed cells). However, it increased to about 250 pmol/ml of...
Stimulating role of potassium ions and ouabain on glycogen synthesis in adipose tissue.
Sobrino, F, Ruiz, G, Goberna, R
1. Exposure of fat-pads to increasing concentrations of K+ in the presence of insulin stimulates the incorporation of labelled glucose into glycogen. In the absence of hormone, only a slight...
Carballo, M, Vilaplana, R, Márquez, G, Conde, M, Bedoya, F J, González-Vílchez, F, ...
To determine the nature of the mechanism by which certain derived ruthenium (Ru) complexes induce regression in tumour growth, we have investigated the possibility that this mechanism was associated...
Cyclosporin A (CsA) is an immunosuppressor without myelotoxicity. It is thought that its effect is mediated by inhibition of Interleukin-2 (IL-2) receptor expression in lymphocytes. We have recently...
Conde, M, Andrade, J, Bedoya, F J, Santa Maria, C, Sobrino, F
Casein-elicited peritoneal macrophages from mice were cultured either alone or with interferon-gamma (IFN-gamma) and bacterial lipopolysaccharide (LPS), and the effect of cyclosporin A (CsA) and...
Alvarez, E, Ruiz-Gutiérrez, V, Sobrino, F, Santa-María, C
The O·2− production has been studied in rat peritoneal neutrophils of different age (3, 12 and 24 months), in order to analyse whether the neutrophil respiratory burst is modified with increasing...