Fernando A. Osorio

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production (2008)

Flores-Mendoza, Lilian, Silva-Campa, Erika, Resendiz, Monica, Osorio, Fernando A., Hernandez, Jesus

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mature dendritic cells (mDCs) derived from porcine monocytes and matured with lipopolysaccharide. The infection of mDCs induced...

Protection against Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Infection through Passive Transfer of PRRSV-Neutralizing Antibodies Is Dose Dependent (2007)

Lopez, O. J., Oliveira, M. F., Alvarez Garcia, E., Kwon, Byung Joon, Doster, Alan R., Osorio, Fernando A.

Previous work in our laboratory demonstrated that passive transfer of porcine reproductive and respiratory syndrome virus (PRRSV)-neutralizing antibodies (NA) protected pregnant sows against...

Influence of N-Linked Glycosylation of Porcine Reproductive and Respiratory Syndrome Virus GP5 on Virus Infectivity, Antigenicity, and Ability To Induce Neutralizing Antibodies (2006)

Ansari, Israrul H., Kwon, Byungjoon, Osorio, Fernando A., Pattnaik, Asit K.

Porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 5 (GP5) is the most abundant envelope glycoprotein and a major inducer of neutralizing antibodies in vivo. Three putative...

Biological responses to porcine respiratory and reproductive syndrome virus in pigs of two genetic populations (2005)

Petry, D. B., Holl, J. W., Weber, J. S., Doster, Alan R., Osorio, Fernando A., Johnson, R. K.

One hundred pigs from the NE Index Line (NEI) and 100 Hampshire-Duroc cross pigs (HD) were inoculated intranasally with porcine respiratory and reproductive syndrome virus (PRRSV 97-7895 strain) at...

Cytokines and synthetic double-stranded RNA augment the T helper 1 immune response of swine to porcine reproductive and respiratory syndrome virus (2004)

Meier, William A., Husmann, Robert J., Schnitzlein, William M., Osorio, Fernando A., Lunney, Joan K., Zuckermann, Federico A.

Immunization of pigs with a modified live porcine reproductive and respiratory syndrome virus (PRRSV) vaccine initially elicits a weak interferon (IFN)-γ response. To improve the immune response, an...

Duration of Infection and Proportion of Pigs Persistently Infected with Porcine Reproductive and Respiratory Syndrome Virus (2003)

Wills, Robert W., Doster, Alan R., Galeota, Judith A., Sur, Jung-Hyang, Osorio, Fernando A.

Understanding the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) persistence in individual pigs is essential to the development of successful control programs. The objectives...

Passive Transfer of Virus-Specific Antibodies Confers Protection against Reproductive Failure Induced by a Virulent Strain of Porcine Reproductive and Respiratory Syndrome Virus and Establishes Sterilizing Immunity (2002)

Osorio, Fernando A., Galeota, J. A., Nelson, E., Brodersen, Bruce W., Doster, Alan R., Wills, R., ...

Immune mechanisms mediating protective immunity against porcine reproductive and respiratory syndrome virus (PRRSV) are not well understood. The PRRSV-specific humoral immune response has been...

Identification of Neutralizing and Nonneutralizing Epitopes in the Porcine Reproductive and Respiratory Syndrome Virus GP5 Ectodomain (2002)

Ostrowski, M., Galeota, J. A., Jar, A. M., Platt, K. B., Osorio, Fernando A., Lopez, O. J.

After infection of swine with porcine reproductive and respiratory syndrome virus (PRRSV), there is a rapid rise of PRRSV-specific nonneutralizing antibodies (NNA), while neutralizing antibodies (NA)...

The Genome of Swinepox Virus (2002)

Afonso, C. L., Tulman, E. R., Lu, Z., Zsak, L., Osorio, Fernando A., Balinsky, C., ...

Swinepox virus (SWPV), the sole member of the Suipoxvirus genus of the Poxviridae, is the etiologic agent of a worldwide disease specific for swine. Here we report the genomic sequence of SWPV. The...

Porcine Reproductive and Respiratory Syndrome Virus: Description of Persistence in Individual Pigs upon Experimental Infection (2000)

Allende, R., Laegreid, W. W., Kutish, G. F., Galeota, J. A., Wills, R. W., Osorio, Fernando A.

We studied the persistence of porcine reproductive and respiratory syndrome virus (PRRSV) in individual experimentally infected pigs, during a period of up to 150 days postinfection (dpi). The...

Duration of PRRS Virus Infections and Proportion of Persistently Infected Pigs (2000)

Wills, R. W., Doster, Alan R., Galeota, J., Osorio, Fernando A.

The objective of this study was to more fully characterize persistent PRRSV infections in swine. Twenty-eight 35-day-old segregated-early-weaned pigs were inoculated intranasally with PRRSV. Serum...

Porcine Reproductive and Respiratory Syndrome Virus Replicates in Testicular Germ Cells, Alters Spermatogenesis, and Induces Germ Cell Death by Apoptosis (1997)

Sur, Jung-Hyang, Doster, Alan R., Christian, James S., Galeota, Judith A., Wills, Robert W., Zimmerman, Jeff J., ...

Like other arteriviruses, porcine reproductive and respiratory syndrome virus (PRRSV) is shed in semen, a feature that is critical for the venereal transmission of this group of viruses. In spite of...

About the Cellular Tropism of the Gammaherpesvirus Bovine Herpesvirus Type 4 (1996)

Osorio, Fernando A., Galeota-Wheeler, J. A., Reed, D. E., Lopez, O.

We read with interest the recent report by Egyed et al. (4) about the distribution in tissue, in the natural host, of bovine herpesvirus type 4 (BHV-4), a member of the gammaherpesvirus group. Using...

In Vivo Detection of Porcine Reproductive and Respiratory Syndrome Virus RNA by In Situ Hybridization at Different Times Post-infection (1996)

Sur, Jung-Hyang, Cooper, Vicki, Galeota, Judith A., Hesse, Richard A., Doster, Alan R., Osorio, Fernando A.

We studied the distribution of porcine reproductive and respiratory syndrome virus (PRRSV) RNA in tissues by in situ hybridization at different times postinfection (p.i.). The probe used for in situ...

Correlation between Precolonization of Trigeminal Ganglia by Attenuated Strains of Pseudorabies Virus and Resistance to Wild-Type Virus Latency (1994)

Schang, L. M., Kutish, G. F., Osorio, Fernando A.

We compared the levels of latent pseudorabies virus (PRV) DNA in trigeminal ganglia (TG) of pigs after intranasal inoculation of different PRV strains by using quantitative DNA PCR. The extent of...

Rapid Detection of Bovine Viral Diarrhea Virus by Polymerase Chain Reaction (1991)

Lopez, O. J., Osorio, Fernando A., Donis, Ruben O.

The polymerase chain reaction was used to detect genomic sequences of the positive-stranded RNA of bovine viral diarrhea virus (BVDV), a member of the family Togaviridae. Using a set of 20-bp primers...

Duration of Infection and Proportion of Pigs Persistently Infected with Porcine Reproductive and Respiratory Syndrome Virus†

Wills, Robert W., Doster, Alan R., Galeota, Judith A., Sur, Jung-Hyang, Osorio, Fernando A.

Understanding the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) persistence in individual pigs is essential to the development of successful control programs. The objectives...

Influence of N-Linked Glycosylation of Porcine Reproductive and Respiratory Syndrome Virus GP5 on Virus Infectivity, Antigenicity, and Ability To Induce Neutralizing Antibodies

Ansari, Israrul H., Kwon, Byungjoon, Osorio, Fernando A., Pattnaik, Asit K.

Porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 5 (GP5) is the most abundant envelope glycoprotein and a major inducer of neutralizing antibodies in vivo. Three putative...

Duration of Infection and Proportion of Pigs Persistently Infected with Porcine Reproductive and Respiratory Syndrome Virus†

Wills, Robert W., Doster, Alan R., Galeota, Judith A., Sur, Jung-Hyang, Osorio, Fernando A.

Understanding the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) persistence in individual pigs is essential to the development of successful control programs. The objectives...

Influence of N-Linked Glycosylation of Porcine Reproductive and Respiratory Syndrome Virus GP5 on Virus Infectivity, Antigenicity, and Ability To Induce Neutralizing Antibodies

Ansari, Israrul H., Kwon, Byungjoon, Osorio, Fernando A., Pattnaik, Asit K.

Porcine reproductive and respiratory syndrome virus (PRRSV) glycoprotein 5 (GP5) is the most abundant envelope glycoprotein and a major inducer of neutralizing antibodies in vivo. Three putative...

Porcine Reproductive and Respiratory Syndrome Virus Infects Mature Porcine Dendritic Cells and Up-Regulates Interleukin-10 Production▿

Flores-Mendoza, Lilian, Silva-Campa, Erika, Reséndiz, Mónica, Osorio, Fernando A., Hernández, Jesús

Porcine reproductive and respiratory syndrome virus (PRRSV) infects mature dendritic cells (mDCs) derived from porcine monocytes and matured with lipopolysaccharide. The infection of mDCs induced...