Filip Pattyn

The emerging molecular pathogenesis of neuroblastoma: implications for improved risk assessment and targeted therapy (2009)

Van Roy, Nadine, De Preter, Katleen, Hoebeeck, Jasmien, Van Maerken, Tom, Pattyn, Filip, Mestdagh, Pieter, ...

Abstract Neuroblastoma is one of the most common solid tumors of childhood, arising from immature sympathetic nervous system cells. The clinical course of patients with neuroblastoma is highly...

Aberrant methylation of candidate tumor suppressor genes in neuroblastoma (2009)

Hoebeeck, Jasmien, MICHELS, EVELIEN, Pattyn, Filip, Combaret, Valerie, Vermeulen, Joelle, Yigit, Nurten, ...

CpG island hypermethylation has been recognized as an alternative mechanism for tumor suppressor gene inactivation. In this study, we performed methylation-specific PCR (MSP) to investigate the...

RDML: structured language and reporting guidelines for real-time quantitative PCR data (2009)

Lefever, Steve, Hellemans, Jan, Pattyn, Filip, Przybylski, Daniel R., Taylor, Chris, Geurts, René, ...

The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exchange of qPCR data and related information between...

RTPrimerDB: the portal for real-time PCR primers and probes (2009)

Lefever, Steve, Vandesompele, Jo, Speleman, Frank, Pattyn, Filip

RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked...

Distinct transcriptional MYCN/c-MYC activities are associated with spontaneous regression or malignant progression in neuroblastomas (2008)

Westermann, Frank, Muth, Daniel, Benner, Axel, Bauer, Tobias, Henrich, Kai-Oliver, Oberthuer, André, ...

Abstract Background Amplified MYCN oncogene resulting in deregulated MYCN transcriptional activity is observed in 20% of neuroblastomas and identifies a highly aggressive subtype. In MYCN single-copy...

BioMed Central (2008)

Bmc Bioinformatics, Filip Pattyn, Jasmien Hoebeeck, Piet Robbrecht, Evi Michels, Anne De, ...

Research article methBLAST and methPrimerDB: web-tools for PCR based methylation analysis

Mapping of 5q35 chromosomal rearrangements within a genomically unstable region (2008)

Buysse, Karen, CREPEL, A, Menten, Björn, Pattyn, Filip, ANTONACCI, F, VELTMAN, J, ...

Background: Recent molecular studies of breakpoints of recurrent chromosome rearrangements revealed the role of genomic architecture in their formation. In particular, segmental duplications...

Distinct transcriptional MYCN/c-MYC activities are associated with spontaneous regression or malignant progression in neuroblastomas (2008)

Westermann, F, Muth, D, Benner, A, Bauer, T, Henrich, KO, Oberthuer, A, ...

Background Amplified MYCN oncogene resulting in deregulated MYCN transcriptional activity is observed in 20% of neuroblastomas and identifies a highly aggressive subtype. In MYCN single-copy...

Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing (2008)

Van Maerken, Tom, Mestdagh, Pieter, De Clercq, Sarah, Pattyn, Filip, Yigit, Nurten, De Paepe, Anne, ...

This article describes how rt-qPCR can be implemented as a tool to monitor silencing efficiency and functional effects of RNA interference (RNAi)-mediated gene knockdown, using examples from our...

methBLAST and methPrimerDB: web-tools for PCR based methylation analysis (2006)

Pattyn, Filip, Hoebeeck, Jasmien, Robbrecht, Piet, Michels, Evi, De Paepe, Anne, Bottu, Guy, ...

Abstract Background DNA methylation plays an important role in development and tumorigenesis by epigenetic modification and silencing of critical genes. The development of PCR-based methylation...

RTPrimerDB: the real-time PCR primer and probe database, major update 2006 (2006)

Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo

The RTPrimerDB (http://medgen.ugent.be/rtprimerdb) project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over...

arrayCGHbase: an analysis platform for comparative genomic hybridization microarrays (2005)

Menten, Björn, Pattyn, Filip, De Preter, Katleen, Robbrecht, Piet, Michels, Evi, Buysse, Karen, ...

Abstract Background The availability of the human genome sequence as well as the large number of physically accessible oligonucleotides, cDNA, and BAC clones across the entire genome has triggered...

Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons (2004)

De Preter, Katleen, Pattyn, Filip, Berx, Geert, Strumane, Kristin, Menten, Björn, Van Roy, Frans, ...

Abstract Background Activation of proto-oncogenes by DNA amplification is an important mechanism in the development and maintenance of cancer cells. Until recently, identification of the targeted...

RTPrimerDB: the real-time PCR primer and probe database (2003)

Filip Pattyn, Frank Speleman, Anne De Paepe, Jo V

The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation is...

BioMed Central (2003)

Bmc Genomics, Katleen De Preter, Filip Pattyn, Geert Berx, Kristin Strumane, Björn Menten, ...

Research article Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons

RTPrimerDB: the Real-Time PCR primer and probe database (2003)

Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo

The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation...

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes (2002)

Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...

Abstract Background Gene-expression analysis is increasingly important in biological research, with real-time reverse transcription PCR (RT-PCR) becoming the method of choice for high-throughput and...

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...

RTPrimerDB: the Real-Time PCR primer and probe database

Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo

The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation is...

RTPrimerDB: the real-time PCR primer and probe database, major update 2006

Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo

The RTPrimerDB () project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over the last year the number of user...

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Vandesompele, Jo, De Preter, Katleen, Pattyn, Filip, Poppe, Bruce, Van Roy, Nadine, De Paepe, Anne, ...

Using real-time reverse transcription PCR ten housekeeping genes from different abundance and functional classes in various human tissues were evaluated. The conventional use of a single gene for...

RTPrimerDB: the Real-Time PCR primer and probe database

Pattyn, Filip, Speleman, Frank, De Paepe, Anne, Vandesompele, Jo

The real-time polymerase chain reaction (PCR) methodology has become increasingly popular for nucleic acids detection and/or quantification. As primer/probe design and experimental evaluation is...

RTPrimerDB: the real-time PCR primer and probe database, major update 2006

Pattyn, Filip, Robbrecht, Piet, De Paepe, Anne, Speleman, Frank, Vandesompele, Jo

The RTPrimerDB () project provides a freely accessible data retrieval system and an in silico assay evaluation pipeline for real-time quantitative PCR assays. Over the last year the number of user...

RDML: structured language and reporting guidelines for real-time quantitative PCR data

Lefever, Steve, Hellemans, Jan, Pattyn, Filip, Przybylski, Daniel R., Taylor, Chris, Geurts, René, ...

The XML-based Real-Time PCR Data Markup Language (RDML) has been developed by the RDML consortium (http://www.rdml.org) to enable straightforward exchange of qPCR data and related information between...

RTPrimerDB: the portal for real-time PCR primers and probes

Lefever, Steve, Vandesompele, Jo, Speleman, Frank, Pattyn, Filip

RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked...

Disease-Causing 7.4 kb Cis-Regulatory Deletion Disrupting Conserved Non-Coding Sequences and Their Interaction with the FOXL2 Promotor: Implications for Mutation Screening

D'haene, Barbara, Attanasio, Catia, Beysen, Diane, Dostie, Josée, Lemire, Edmond, Bouchard, Philippe, ...

To date, the contribution of disrupted potentially cis-regulatory conserved non-coding sequences (CNCs) to human disease is most likely underestimated, as no systematic screens for putative...

The emerging molecular pathogenesis of neuroblastoma: implications for improved risk assessment and targeted therapy

Van Roy, Nadine, De Preter, Katleen, Hoebeeck, Jasmien, Van Maerken, Tom, Pattyn, Filip, Mestdagh, Pieter, ...

Neuroblastoma is one of the most common solid tumors of childhood, arising from immature sympathetic nervous system cells. The clinical course of patients with neuroblastoma is highly variable,...