Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease (2009)
Grizot, Sylvestre, Smith, Julianne, Daboussi, Fayza, Prieto, Jesús, Redondo, Pilar, Merino, Nekane, ...
Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to...
Fajardo-Sanchez, Emmanuel, Stricher, François, Pâques, Frédéric, Isalan, Mark, Serrano, Luis
Meganucleases cut long (>12 bp) unique sequences in genomes and can be used to induce targeted genome engineering by homologous recombination in the vicinity of their cleavage site. However, the use...
Prieto, Jesús, Redondo, Pilar, Padró, Daniel, Arnould, Sylvain, Epinat, Jean-Charles, Pâques, Frédéric, ...
Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel...
Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric
Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...
A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells (2003)
Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...
Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site‐ specific DNA double‐strand breaks...
Sugawara, Neal, Pâques, Frédéric, Colaiácovo, Mónica, Haber, James E.
When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. In Saccharomyces...
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Pâques, Frédéric, Haber, James E.
The budding yeast Saccharomyces cerevisiae has been the principal organism used in experiments to examine genetic recombination in eukaryotes. Studies over the past decade have shown that meiotic...
Expansions and Contractions in a Tandem Repeat Induced by Double-Strand Break Repair
Pâques, Frédéric, Leung, Wai-Ying, Haber, James E.
Repair of a double-strand break (DSB) in yeast can induce very frequent expansions and contractions in a tandem array of 375-bp repeats. These results strongly suggest that DSB repair can be a major...
A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells
Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...
Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...
Mini- and microsatellite expansions: the recombination connection
Richard, Guy-Franck, Pâques, Frédéric
It is widely accepted that the large trinucleotide repeat expansions observed in many neurological diseases occur during replication. However, genetic recombination has emerged as a major source of...
Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric
Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...
Sugawara, Neal, Pâques, Frédéric, Colaiácovo, Mónica, Haber, James E.
When gene conversion is initiated by a double-strand break (DSB), any nonhomologous DNA that may be present at the ends must be removed before new DNA synthesis can be initiated. In Saccharomyces...
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae
Pâques, Frédéric, Haber, James E.
The budding yeast Saccharomyces cerevisiae has been the principal organism used in experiments to examine genetic recombination in eukaryotes. Studies over the past decade have shown that meiotic...
Expansions and Contractions in a Tandem Repeat Induced by Double-Strand Break Repair
Pâques, Frédéric, Leung, Wai-Ying, Haber, James E.
Repair of a double-strand break (DSB) in yeast can induce very frequent expansions and contractions in a tandem array of 375-bp repeats. These results strongly suggest that DSB repair can be a major...
A novel engineered meganuclease induces homologous recombination in yeast and mammalian cells
Epinat, Jean-Charles, Arnould, Sylvain, Chames, Patrick, Rochaix, Pascal, Desfontaines, Dominique, Puzin, Clémence, ...
Homologous gene targeting is the ultimate tool for reverse genetics, but its use is often limited by low efficiency. In a number of recent studies, site- specific DNA double-strand breaks (DSBs) have...
Mini- and microsatellite expansions: the recombination connection
Richard, Guy-Franck, Pâques, Frédéric
It is widely accepted that the large trinucleotide repeat expansions observed in many neurological diseases occur during replication. However, genetic recombination has emerged as a major source of...
Chames, Patrick, Epinat, Jean-Charles, Guillier, Sophie, Patin, Amélie, Lacroix, Emmanuel, Pâques, Frédéric
Homing endonucleases, endonucleases capable of recognizing long DNA sequences, have been shown to be a tool of choice for precise and efficient genome engineering. Consequently, the possibility to...
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences
Smith, Julianne, Grizot, Sylvestre, Arnould, Sylvain, Duclert, Aymeric, Epinat, Jean-Charles, Chames, Patrick, ...
Meganucleases, or homing endonucleases (HEs) are sequence-specific endonucleases with large (>14 bp) cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells...
Prieto, Jesús, Redondo, Pilar, Padró, Daniel, Arnould, Sylvain, Epinat, Jean-Charles, Pâques, Frédéric, ...
Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel...
Fajardo-Sanchez, Emmanuel, Stricher, François, Pâques, Frédéric, Isalan, Mark, Serrano, Luis
Meganucleases cut long (>12 bp) unique sequences in genomes and can be used to induce targeted genome engineering by homologous recombination in the vicinity of their cleavage site. However, the use...
Marcaida, María José, Prieto, Jesús, Redondo, Pilar, Nadra, Alejandro D., Alibés, Andreu, Serrano, Luis, ...
Homing endonucleases, also known as meganucleases, are sequence-specific enzymes with large DNA recognition sites. These enzymes can be used to induce efficient homologous gene targeting in cells and...
Efficient targeting of a SCID gene by an engineered single-chain homing endonuclease
Grizot, Sylvestre, Smith, Julianne, Daboussi, Fayza, Prieto, Jesús, Redondo, Pilar, Merino, Nekane, ...
Sequence-specific endonucleases recognizing long target sequences are emerging as powerful tools for genome engineering. These endonucleases could be used to correct deleterious mutations or to...