François-Loïc Cosset

Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+bone marrow cells, following gamma irradiation in cynomolgus macaques (2008)

Derdouch, Sonia, Gay, Wilfried, Nègre, Didier, Prost, Stéphane, Le Dantec, Mikael, Delache, Benoît, ...

Abstract Background Prolonged, altered hematopoietic reconstitution is commonly observed in patients undergoing myeloablative conditioning and bone marrow and/or mobilized peripheral blood-derived...

Host neutralizing responses and pathogenesis of hepatitis C virus infection. (2008)

Zeisel, Mirjam, Cosset, François-Loïc, Baumert, Thomas

The recent development of novel model systems for the early steps of hepatitis C virus (HCV) infection has rapidly advanced our knowledge of antibody-mediated virus neutralization in patients with...

Host neutralizing responses and pathogenesis of hepatitis C virus infection. (2008)

Zeisel, Mirjam, Cosset, François-Loïc, Baumert, Thomas

The recent development of novel model systems for the early steps of hepatitis C virus (HCV) infection has rapidly advanced our knowledge of antibody-mediated virus neutralization in patients with...

Host neutralizing responses and pathogenesis of hepatitis C virus infection. (2008)

Zeisel, Mirjam, Cosset, François-Loïc, Baumert, Thomas

The recent development of novel model systems for the early steps of hepatitis C virus (HCV) infection has rapidly advanced our knowledge of antibody-mediated virus neutralization in patients with...

Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C. (2007)

Pestka, Jan, Zeisel, Mirjam, Bläser, Edith, Schürmann, Peter, Bartosch, Birke, Cosset, François-Loïc, ...

In contrast to a detailed understanding of antiviral cellular immune responses, the impact of neutralizing antibodies for the resolution of acute hepatitis C is poorly defined. The analysis of...

Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C. (2007)

Pestka, Jan, Zeisel, Mirjam, Bläser, Edith, Schürmann, Peter, Bartosch, Birke, Cosset, François-Loïc, ...

In contrast to a detailed understanding of antiviral cellular immune responses, the impact of neutralizing antibodies for the resolution of acute hepatitis C is poorly defined. The analysis of...

Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C. (2007)

Pestka, Jan, Zeisel, Mirjam, Bläser, Edith, Schürmann, Peter, Bartosch, Birke, Cosset, François-Loïc, ...

In contrast to a detailed understanding of antiviral cellular immune responses, the impact of neutralizing antibodies for the resolution of acute hepatitis C is poorly defined. The analysis of...

Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81. (2007)

Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...

Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81. (2007)

Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...

Scavenger receptor class B type I is a key host factor for hepatitis C virus infection required for an entry step closely linked to CD81. (2007)

Zeisel, Mirjam, Koutsoudakis, George, Schnober, Eva, Haberstroh, Anita, Blum, Hubert, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Scavenger receptor class B type I (SR-BI) has been shown to bind HCV envelope glycoprotein E2, participate in entry of HCV...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses (2006)

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

Summary There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles...

Cell cycle features of primate embryonic stem cells. (2006)

Fluckiger, Anne-Catherine, Marcy, Guillaume, Marchand, Mélanie, Négre, Didier, Cosset, François-Loïc, Mitalipov, Shoukhrat, ...

Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. (2006)

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

SUMMARY: There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. (2006)

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

SUMMARY: There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles...

Cell cycle features of primate embryonic stem cells. (2006)

Fluckiger, Anne-Catherine, Marcy, Guillaume, Marchand, Mélanie, Négre, Didier, Cosset, François-Loïc, Mitalipov, Shoukhrat, ...

Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit...

Cell cycle features of primate embryonic stem cells. (2006)

Fluckiger, Anne-Catherine, Marcy, Guillaume, Marchand, Mélanie, Négre, Didier, Cosset, François-Loïc, Mitalipov, Shoukhrat, ...

Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. (2006)

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

SUMMARY: There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles...

Cell cycle features of primate embryonic stem cells. (2006)

Fluckiger, Anne-Catherine, Marcy, Guillaume, Marchand, Mélanie, Negre, Didier, Cosset, François-Loïc, Mitalipov, Shoukhrat, ...

Using flow cytometry measurements combined with quantitative analysis of cell cycle kinetics, we show that rhesus monkey embryonic stem cells (ESCs) are characterized by an extremely rapid transit...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses. (2006)

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

SUMMARY: There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles...

A universal transgene silencing method based on RNA interference (2004)

Mangeot, Philippe-Emmanuel, Cosset, François-Loïc, Colas, Pierre, Mikaelian, Ivan

Inducible gene expression systems have contributed significantly to the understanding of molecular regulatory networks. Here we describe a simple and powerful RNA interference-based method that can...

Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. (2003)

Bartosch, Birke, Dubuisson, Jean, Cosset, François-Loïc

The study of hepatitis C virus (HCV), a major cause of chronic liver disease, has been hampered by the lack of a cell culture system supporting its replication. Here, we have successfully generated...

IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes. (2003)

Verhoeyen, Els, Dardalhon, Valerie, Ducrey-Rundquist, Odile, Trono, Didier, Taylor, Naomi, Cosset, François-Loïc

Important gene therapy target cells such as resting human T cells are refractory to transduction with lentiviral vectors. Completion of reverse transcription, nuclear import, and subsequent...

Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. (2003)

Bartosch, Birke, Dubuisson, Jean, Cosset, François-Loïc

The study of hepatitis C virus (HCV), a major cause of chronic liver disease, has been hampered by the lack of a cell culture system supporting its replication. Here, we have successfully generated...

Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes. (2003)

Bartosch, Birke, Dubuisson, Jean, Cosset, François-Loïc

The study of hepatitis C virus (HCV), a major cause of chronic liver disease, has been hampered by the lack of a cell culture system supporting its replication. Here, we have successfully generated...

Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates. (2002)

Sandrin, Virginie, Boson, Bertrand, Salmon, Patrick, Gay, Wilfried, Nègre, Didier, Le Grand, Roger, ...

Generating lentiviral vectors pseudotyped with different viral glycoproteins (GPs) may modulate the physicochemical properties of the vectors, their interaction with the host immune system, and their...

Efficient gene transfer into human primary blood lymphocytes by surface-engineered lentiviral vectors that display a T cell-activating polypeptide. (2002)

Maurice, Marielle, Verhoeyen, Els, Salmon, Patrick, Trono, Didier, Russell, Stephen J, Cosset, François-Loïc

In contrast to oncoretroviruses, lentiviruses such as human immunodeficiency virus 1 (HIV-1) are able to integrate their genetic material into the genome of nonproliferating cells that are...

Stable micro-dystrophin gene transfer using an integrating adeno-retroviral hybrid vector ameliorates the dystrophic pathology in mdx mouse muscle (2002)

Roberts, Michael L., Wells, Dominic J., Graham, Ian R., Fabb, Stewart A., Hill, Vanessa J., Duisit, Ghislaine, ...

The ability to transfer the dystrophin gene stably to the skeletal muscle of DMD patients is a major confounding issue in establishing an effective gene therapy for this disease. To overcome this...

A Proline-Rich Motif Downstream of the Receptor Binding Domain Modulates Conformation and Fusogenicity of Murine Retroviral Envelopes

Lavillette, Dimitri, Maurice, Marielle, Roche, Catherine, Russell, Stephen J., Sitbon, Marc, Cosset, François-Loïc

The entry of retroviruses into cells depends on receptor recognition by the viral envelope surface subunit SU followed by membrane fusion, which is thought to be mediated by a fusion peptide located...

Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments

Lavillette, Dimitri, Ruggieri, Alessia, Russell, Stephen J., Cosset, François-Loïc

Mutations that negatively or positively affect the fusion properties of murine leukemia viruses (MLVs) have been found within all subdomains of their SU (surface) and TM (transmembrane) envelope...

An Envelope Glycoprotein of the Human Endogenous Retrovirus HERV-W Is Expressed in the Human Placenta and Fuses Cells Expressing the Type D Mammalian Retrovirus Receptor

Blond, Jean-Luc, Lavillette, Dimitri, Cheynet, Valérie, Bouton, Olivier, Oriol, Guy, Chapel-Fernandes, Sylvie, ...

A new human endogenous retrovirus (HERV) family, termed HERV-W, was recently described (J.-L. Blond, F. Besème, L. Duret, O. Bouton, F. Bedin, H. Perron, B. Mandrand, and F. Mallet, J. Virol....

Retroviral Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus

Miletic, Hrvoje, Bruns, Michael, Tsiakas, Konstantinos, Vogt, Birgit, Rezai, Roya, Baum, Christopher, ...

Pseudotyping can improve retroviral vector stability and transduction efficiency. Here, we describe a novel pseudotype of murine leukemia virus packaged with lymphocytic choriomeningitis virus...

Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface Glycoprotein

Lavillette, Dimitri, Boson, Bertrand, Russell, Stephen J., Cosset, François-Loïc

Cell entry of retroviruses is initiated by the recognition of cellular receptors and the subsequent membrane fusion between viral and cellular membranes. These two steps are mediated by the surface...

Development of Minimal Lentivirus Vectors Derived from Simian Immunodeficiency Virus (SIVmac251) and Their Use for Gene Transfer into Human Dendritic Cells

Mangeot, Philippe-Emmanuel, Nègre, Didier, Dubois, Bertrand, Winter, Arend J., Leissner, Philippe, Mehtali, Majid, ...

Lentivirus-derived vectors are very promising gene delivery systems since they are able to transduce nonproliferating differentiated cells, while murine leukemia virus-based vectors can only...

Incorporation of Fowl Plague Virus Hemagglutinin into Murine Leukemia Virus Particles and Analysis of the Infectivity of the Pseudotyped Retroviruses

Hatziioannou, Theodora, Valsesia-Wittmann, Sandrine, Russell, Stephen J., Cosset, François-Loïc

We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and a lacZ...

A New System for Stringent, High-Titer Vesicular Stomatitis Virus G Protein-Pseudotyped Retrovirus Vector Induction by Introduction of Cre Recombinase into Stable Prepackaging Cell Lines

Arai, Tohru, Matsumoto, Kazuyuki, Saitoh, Kanako, Ui, Motoyasu, Ito, Taiji, Murakami, Masao, ...

We report here on stable prepackaging cell lines which can be converted into packaging cell lines for high-titer vesicular stomatitis virus G protein (VSV-G)-pseudotyped retrovirus vectors by the...

The Envelope Glycoprotein of Human Endogenous Retrovirus Type W Uses a Divergent Family of Amino Acid Transporters/Cell Surface Receptors

Lavillette, Dimitri, Marin, Mariana, Ruggieri, Alessia, Mallet, François, Cosset, François-Loïc, Kabat, David

The human endogenous retrovirus type W (HERV-W) family includes proviruses with intact protein-coding regions that appear to be under selection pressure, suggesting that some HERV-W proviruses may...

Relationship between SU Subdomains That Regulate the Receptor-Mediated Transition from the Native (Fusion-Inhibited) to the Fusion-Active Conformation of the Murine Leukemia Virus Glycoprotein

Lavillette, Dimitri, Ruggieri, Alessia, Boson, Bertrand, Maurice, Marielle, Cosset, François-Loïc

Envelope glycoproteins (Env) of retroviruses are trimers of SU (surface) and TM (transmembrane) heterodimers and are expressed on virions in fusion-competent forms that are likely to be metastable....

In vitro assay for neutralizing antibody to hepatitis C virus: Evidence for broadly conserved neutralization epitopes

Bartosch, Birke, Bukh, Jens, Meunier, Jean-Christophe, Granier, Christelle, Engle, Ronald E., Blackwelder, William C., ...

Our understanding of the humoral immune response to hepatitis C virus (HCV) is limited because the virus can be studied only in humans and chimpanzees and because previously described neutralization...

Characterization of Functional Hepatitis C Virus Envelope Glycoproteins

Op De Beeck, Anne, Voisset, Cécile, Bartosch, Birke, Ciczora, Yann, Cocquerel, Laurence, Keck, Zhenyong, ...

Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that assemble as a noncovalent heterodimer which is mainly retained in the endoplasmic reticulum. Because assembly into...

Neutralizing antibodies to hepatitis C virus (HCV) in immune globulins derived from anti-HCV-positive plasma

Yu, Mei-ying W., Bartosch, Birke, Zhang, Pei, Guo, Zheng-ping, Renzi, Paula M., Shen, Li-ming, ...

The role of humoral immunity in hepatitis C virus (HCV) infections is uncertain. Nevertheless, there is increasing evidence for neutralizing antibodies to HCV in the serum or plasma of chronically...

Intracellular Trafficking of Gag and Env Proteins and Their Interactions Modulate Pseudotyping of Retroviruses

Sandrin, Virginie, Muriaux, Delphine, Darlix, Jean-Luc, Cosset, François-Loïc

Glycoproteins derived from most retroviruses and from several families of enveloped viruses can form infectious pseudotypes with murine leukemia virus (MLV) and lentiviral core particles, like the...

A universal transgene silencing method based on RNA interference

Mangeot, Philippe-Emmanuel, Cosset, François-Loïc, Colas, Pierre, Mikaelian, Ivan

Inducible gene expression systems have contributed significantly to the understanding of molecular regulatory networks. Here we describe a simple and powerful RNA interference-based method that can...

Human Serum Facilitates Hepatitis C Virus Infection, and Neutralizing Responses Inversely Correlate with Viral Replication Kinetics at the Acute Phase of Hepatitis C Virus Infection

Lavillette, Dimitri, Morice, Yoann, Germanidis, Georgios, Donot, Peggy, Soulier, Alexandre, Pagkalos, Emanuil, ...

The factors leading to spontaneous clearance of hepatitis C virus (HCV) or to viral persistence are elusive. Understanding virus-host interactions that enable acute HCV clearance is key to the...

Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins

Goffard, Anne, Callens, Nathalie, Bartosch, Birke, Wychowski, Czeslaw, Cosset, François-Loïc, Montpellier, Claire, ...

Hepatitis C virus (HCV) encodes two viral envelope glycoproteins. E1 contains 4 or 5 N-linked glycosylation sites and E2 contains up to 11, with most of the sites being well conserved, suggesting...

Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein

Owsianka, Ania, Tarr, Alexander W., Juttla, Vicky S., Lavillette, Dimitri, Bartosch, Birke, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) remains a significant threat to the general health of the world's population, and there is a pressing need for the development of new treatments and preventative vaccines....

Analysis of a Highly Flexible Conformational Immunogenic Domain A in Hepatitis C Virus E2

Keck, Zhen-Yong, Li, Ta-Kai, Xia, Jinming, Bartosch, Birke, Cosset, François-Loïc, Dubuisson, Jean, ...

Hepatitis C (HCV) E2 glycoprotein is involved in virus attachment and entry, and its structural organization is largely unknown. Characterization of a panel of human monoclonal antibodies (HMAbs) to...

Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry

Callens, Nathalie, Ciczora, Yann, Bartosch, Birke, Vu-Dac, Ngoc, Cosset, François-Loïc, Pawlotsky, Jean-Michel, ...

The N terminus of hepatitis C virus (HCV) envelope glycoprotein E2 contains a hypervariable region (HVR1) which has been proposed to play a role in viral entry. Despite strong amino acid variability,...

Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry

Broer, Rene, Boson, Bertrand, Spaan, Willy, Cosset, François-Loïc, Corver, Jeroen

The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles (Flu-VLPs)...

A Proline-Rich Motif Downstream of the Receptor Binding Domain Modulates Conformation and Fusogenicity of Murine Retroviral Envelopes

Lavillette, Dimitri, Maurice, Marielle, Roche, Catherine, Russell, Stephen J., Sitbon, Marc, Cosset, François-Loïc

The entry of retroviruses into cells depends on receptor recognition by the viral envelope surface subunit SU followed by membrane fusion, which is thought to be mediated by a fusion peptide located...

Activation of a Cell Entry Pathway Common to Type C Mammalian Retroviruses by Soluble Envelope Fragments

Lavillette, Dimitri, Ruggieri, Alessia, Russell, Stephen J., Cosset, François-Loïc

Mutations that negatively or positively affect the fusion properties of murine leukemia viruses (MLVs) have been found within all subdomains of their SU (surface) and TM (transmembrane) envelope...

An Envelope Glycoprotein of the Human Endogenous Retrovirus HERV-W Is Expressed in the Human Placenta and Fuses Cells Expressing the Type D Mammalian Retrovirus Receptor

Blond, Jean-Luc, Lavillette, Dimitri, Cheynet, Valérie, Bouton, Olivier, Oriol, Guy, Chapel-Fernandes, Sylvie, ...

A new human endogenous retrovirus (HERV) family, termed HERV-W, was recently described (J.-L. Blond, F. Besème, L. Duret, O. Bouton, F. Bedin, H. Perron, B. Mandrand, and F. Mallet, J. Virol....

Retroviral Vectors Pseudotyped with Lymphocytic Choriomeningitis Virus

Miletic, Hrvoje, Bruns, Michael, Tsiakas, Konstantinos, Vogt, Birgit, Rezai, Roya, Baum, Christopher, ...

Pseudotyping can improve retroviral vector stability and transduction efficiency. Here, we describe a novel pseudotype of murine leukemia virus packaged with lymphocytic choriomeningitis virus...

Activation of Membrane Fusion by Murine Leukemia Viruses Is Controlled in cis or in trans by Interactions between the Receptor-Binding Domain and a Conserved Disulfide Loop of the Carboxy Terminus of the Surface Glycoprotein

Lavillette, Dimitri, Boson, Bertrand, Russell, Stephen J., Cosset, François-Loïc

Cell entry of retroviruses is initiated by the recognition of cellular receptors and the subsequent membrane fusion between viral and cellular membranes. These two steps are mediated by the surface...

Development of Minimal Lentivirus Vectors Derived from Simian Immunodeficiency Virus (SIVmac251) and Their Use for Gene Transfer into Human Dendritic Cells

Mangeot, Philippe-Emmanuel, Nègre, Didier, Dubois, Bertrand, Winter, Arend J., Leissner, Philippe, Mehtali, Majid, ...

Lentivirus-derived vectors are very promising gene delivery systems since they are able to transduce nonproliferating differentiated cells, while murine leukemia virus-based vectors can only...

Incorporation of Fowl Plague Virus Hemagglutinin into Murine Leukemia Virus Particles and Analysis of the Infectivity of the Pseudotyped Retroviruses

Hatziioannou, Theodora, Valsesia-Wittmann, Sandrine, Russell, Stephen J., Cosset, François-Loïc

We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and a lacZ...

A New System for Stringent, High-Titer Vesicular Stomatitis Virus G Protein-Pseudotyped Retrovirus Vector Induction by Introduction of Cre Recombinase into Stable Prepackaging Cell Lines

Arai, Tohru, Matsumoto, Kazuyuki, Saitoh, Kanako, Ui, Motoyasu, Ito, Taiji, Murakami, Masao, ...

We report here on stable prepackaging cell lines which can be converted into packaging cell lines for high-titer vesicular stomatitis virus G protein (VSV-G)-pseudotyped retrovirus vectors by the...

The Envelope Glycoprotein of Human Endogenous Retrovirus Type W Uses a Divergent Family of Amino Acid Transporters/Cell Surface Receptors

Lavillette, Dimitri, Marin, Mariana, Ruggieri, Alessia, Mallet, François, Cosset, François-Loïc, Kabat, David

The human endogenous retrovirus type W (HERV-W) family includes proviruses with intact protein-coding regions that appear to be under selection pressure, suggesting that some HERV-W proviruses may...

Relationship between SU Subdomains That Regulate the Receptor-Mediated Transition from the Native (Fusion-Inhibited) to the Fusion-Active Conformation of the Murine Leukemia Virus Glycoprotein

Lavillette, Dimitri, Ruggieri, Alessia, Boson, Bertrand, Maurice, Marielle, Cosset, François-Loïc

Envelope glycoproteins (Env) of retroviruses are trimers of SU (surface) and TM (transmembrane) heterodimers and are expressed on virions in fusion-competent forms that are likely to be metastable....

In vitro assay for neutralizing antibody to hepatitis C virus: Evidence for broadly conserved neutralization epitopes

Bartosch, Birke, Bukh, Jens, Meunier, Jean-Christophe, Granier, Christelle, Engle, Ronald E., Blackwelder, William C., ...

Our understanding of the humoral immune response to hepatitis C virus (HCV) is limited because the virus can be studied only in humans and chimpanzees and because previously described neutralization...

Characterization of Functional Hepatitis C Virus Envelope Glycoproteins

Op De Beeck, Anne, Voisset, Cécile, Bartosch, Birke, Ciczora, Yann, Cocquerel, Laurence, Keck, Zhenyong, ...

Hepatitis C virus (HCV) encodes two envelope glycoproteins, E1 and E2, that assemble as a noncovalent heterodimer which is mainly retained in the endoplasmic reticulum. Because assembly into...

Neutralizing antibodies to hepatitis C virus (HCV) in immune globulins derived from anti-HCV-positive plasma

Yu, Mei-ying W., Bartosch, Birke, Zhang, Pei, Guo, Zheng-ping, Renzi, Paula M., Shen, Li-ming, ...

The role of humoral immunity in hepatitis C virus (HCV) infections is uncertain. Nevertheless, there is increasing evidence for neutralizing antibodies to HCV in the serum or plasma of chronically...

Intracellular Trafficking of Gag and Env Proteins and Their Interactions Modulate Pseudotyping of Retroviruses

Sandrin, Virginie, Muriaux, Delphine, Darlix, Jean-Luc, Cosset, François-Loïc

Glycoproteins derived from most retroviruses and from several families of enveloped viruses can form infectious pseudotypes with murine leukemia virus (MLV) and lentiviral core particles, like the...

A universal transgene silencing method based on RNA interference

Mangeot, Philippe-Emmanuel, Cosset, François-Loïc, Colas, Pierre, Mikaelian, Ivan

Inducible gene expression systems have contributed significantly to the understanding of molecular regulatory networks. Here we describe a simple and powerful RNA interference-based method that can...

Human Serum Facilitates Hepatitis C Virus Infection, and Neutralizing Responses Inversely Correlate with Viral Replication Kinetics at the Acute Phase of Hepatitis C Virus Infection

Lavillette, Dimitri, Morice, Yoann, Germanidis, Georgios, Donot, Peggy, Soulier, Alexandre, Pagkalos, Emanuil, ...

The factors leading to spontaneous clearance of hepatitis C virus (HCV) or to viral persistence are elusive. Understanding virus-host interactions that enable acute HCV clearance is key to the...

Role of N-Linked Glycans in the Functions of Hepatitis C Virus Envelope Glycoproteins

Goffard, Anne, Callens, Nathalie, Bartosch, Birke, Wychowski, Czeslaw, Cosset, François-Loïc, Montpellier, Claire, ...

Hepatitis C virus (HCV) encodes two viral envelope glycoproteins. E1 contains 4 or 5 N-linked glycosylation sites and E2 contains up to 11, with most of the sites being well conserved, suggesting...

Monoclonal Antibody AP33 Defines a Broadly Neutralizing Epitope on the Hepatitis C Virus E2 Envelope Glycoprotein

Owsianka, Ania, Tarr, Alexander W., Juttla, Vicky S., Lavillette, Dimitri, Bartosch, Birke, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) remains a significant threat to the general health of the world's population, and there is a pressing need for the development of new treatments and preventative vaccines....

Analysis of a Highly Flexible Conformational Immunogenic Domain A in Hepatitis C Virus E2

Keck, Zhen-Yong, Li, Ta-Kai, Xia, Jinming, Bartosch, Birke, Cosset, François-Loïc, Dubuisson, Jean, ...

Hepatitis C (HCV) E2 glycoprotein is involved in virus attachment and entry, and its structural organization is largely unknown. Characterization of a panel of human monoclonal antibodies (HMAbs) to...

Basic Residues in Hypervariable Region 1 of Hepatitis C Virus Envelope Glycoprotein E2 Contribute to Virus Entry

Callens, Nathalie, Ciczora, Yann, Bartosch, Birke, Vu-Dac, Ngoc, Cosset, François-Loïc, Pawlotsky, Jean-Michel, ...

The N terminus of hepatitis C virus (HCV) envelope glycoprotein E2 contains a hypervariable region (HVR1) which has been proposed to play a role in viral entry. Despite strong amino acid variability,...

Important Role for the Transmembrane Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Protein during Entry

Broer, Rene, Boson, Bertrand, Spaan, Willy, Cosset, François-Loïc, Corver, Jeroen

The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that...

Induction of neutralising antibodies by virus-like particles harbouring surface proteins from highly pathogenic H5N1 and H7N1 influenza viruses

Szécsi, Judit, Boson, Bertrand, Johnsson, Per, Dupeyrot-Lacas, Pia, Matrosovich, Mikhail, Klenk, Hans-Dieter, ...

There is an urgent need to develop novel approaches to vaccination against the emerging, highly pathogenic avian influenza viruses. Here, we engineered influenza viral-like particles (Flu-VLPs)...

Characterization of Fusion Determinants Points to the Involvement of Three Discrete Regions of Both E1 and E2 Glycoproteins in the Membrane Fusion Process of Hepatitis C Virus▿

Lavillette, Dimitri, Pécheur, Eve-Isabelle, Donot, Peggy, Fresquet, Judith, Molle, Jennifer, Corbau, Romuald, ...

Infection of eukaryotic cells by enveloped viruses requires the merging of viral and cellular membranes. Highly specific viral surface glycoproteins, named fusion proteins, catalyze this reaction by...

Infectious Hepatitis C Virus Pseudo-particles Containing Functional E1–E2 Envelope Protein Complexes

Bartosch, Birke, Dubuisson, Jean, Cosset, François-Loïc

The study of hepatitis C virus (HCV), a major cause of chronic liver disease, has been hampered by the lack of a cell culture system supporting its replication. Here, we have successfully generated...

Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques

Derdouch, Sonia, Gay, Wilfried, Nègre, Didier, Prost, Stéphane, Le Dantec, Mikael, Delache, Benoît, ...

The Tight Junction Proteins Claudin-1, -6, and -9 Are Entry Cofactors for Hepatitis C Virus▿

Meertens, Laurent, Bertaux, Claire, Cukierman, Lisa, Cormier, Emmanuel, Lavillette, Dimitri, Cosset, François-Loïc, ...

Hepatitis C virus (HCV) is a major cause of liver disease in humans. The CD81 tetraspanin is necessary but not sufficient for HCV penetration into hepatocytes, and it was recently reported that the...

Receptor Complementation and Mutagenesis Reveal SR-BI as an Essential HCV Entry Factor and Functionally Imply Its Intra- and Extra-Cellular Domains

Dreux, Marlène, Dao Thi, Viet Loan, Fresquet, Judith, Guérin, Maryse, Julia, Zélie, Verney, Géraldine, ...

HCV entry into cells is a multi-step and slow process. It is believed that the initial capture of HCV particles by glycosaminoglycans and/or lipoprotein receptors is followed by coordinated...

Ciliary Beating Recovery in Deficient Human Airway Epithelial Cells after Lentivirus Ex Vivo Gene Therapy

Chhin, Brigitte, Negre, Didier, Merrot, Olivier, Pham, Jacqueline, Tourneur, Yves, Ressnikoff, Denis, ...

Primary Ciliary Dyskinesia is a heterogeneous genetic disease that is characterized by cilia dysfunction of the epithelial cells lining the respiratory tracts, resulting in recurrent respiratory...