Francisco J. Iborra

Can visco-elastic phase separation, macromolecular crowding and colloidal physics explain nuclear organisation? (2007)

Iborra, Francisco J

Abstract Background The cell nucleus is highly compartmentalized with well-defined domains, it is not well understood how this nuclear order is maintained. Many scientists are fascinated by the...

The functional organization of mitochondrial genomes in human cells (2004)

Iborra, Francisco J, Kimura, Hiroshi, Cook, Peter R

Abstract Background We analyzed the organization and function of mitochondrial DNA in a stable human cell line (ECV304, which is also known as T-24) containing mitochondria tagged with the yellow...

Numbers and Organization of RNA Polymerases, Nascent Transcripts, and Transcription Units in HeLa Nuclei

Jackson, Dean A., Iborra, Francisco J., Manders, Erik M.M., Cook, Peter R.

Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases...

Numbers and Organization of RNA Polymerases, Nascent Transcripts, and Transcription Units in HeLa Nuclei

Jackson, Dean A., Iborra, Francisco J., Manders, Erik M.M., Cook, Peter R.

Using HeLa cells, we have developed methods to determine 1) the number of RNA polymerases that are active at any moment, 2) the number of transcription sites, and 3) the number of polymerases...

Coregulated human globin genes are frequently in spatial proximity when active

Brown, Jill M., Leach, Joanne, Reittie, Joyce E., Atzberger, Ann, Lee-Prudhoe, Jane, Wood, William G., ...

The organization of genes within the nucleus may influence transcription. We have analyzed the nuclear positioning of the coordinately regulated α- and β-globin genes and show that the gene-dense...

A novel histone exchange factor, protein phosphatase 2Cγ, mediates the exchange and dephosphorylation of H2A–H2B

Kimura, Hiroshi, Takizawa, Nanako, Allemand, Eric, Hori, Tetsuya, Iborra, Francisco J., Nozaki, Naohito, ...

In eukaryotic nuclei, DNA is wrapped around a protein octamer composed of the core histones H2A, H2B, H3, and H4, forming nucleosomes as the fundamental units of chromatin. The modification and...

Association between active genes occurs at nuclear speckles and is modulated by chromatin environment

Brown, Jill M., Green, Joanne, Das Neves, Ricardo Pires, Wallace, Helen A.C., Smith, Andrew J.H., Hughes, Jim, ...

Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains...