Franki Speleman

Aberrant methylation of candidate tumor suppressor genes in neuroblastoma (2009)

Hoebeeck, Jasmien, MICHELS, EVELIEN, Pattyn, Filip, Combaret, Valerie, Vermeulen, Joelle, Yigit, Nurten, ...

CpG island hypermethylation has been recognized as an alternative mechanism for tumor suppressor gene inactivation. In this study, we performed methylation-specific PCR (MSP) to investigate the...

Unusual 8p inverted duplication deletion with telomere capture from 8q (2009)

Buysse, Karen, Antonacci, F, CALLEWAERT, BERT, Loeys, Bart, Fränkel, Ulrike, Siu, Victoria, ...

Inverted 8p duplication deletions are recurrent chromosomal rearrangements that are mediated through non-allelic homologous recombination (NAHR) between olfactory receptor (OR) gene clusters at...

Genome profiling of acute myelomonocytic leukemia: alteration of the MYB locus in MYST3-linked cases (2009)

Murati, A, Gervais, C, Carbuccia, N, Finetti, P, Cervera, N, Adelaide, J, ...

The t(8; 16)(p11; p13) is a rare translocation involved in de novo and therapy-related myelomonocytic and monocytic acute leukemia. It fuses two genes encoding histone acetyltransferases (HATs),...

The 12q14 microdeletion syndrome: additional patients and further evidence that HMGA2 is an important genetic determinant for human height (2009)

Buysse, Karen, Reardon, William, Mehta, Lakshmi, Costa, Teresa, Fagerstrom, Carrie, Kingsbury, Daniel J., ...

Characteristic features of the 12q14 microdeletion syndrome include low birth weight, failure to thrive, short stature, learning disabilities and Buschke-Ollendorff lesions in bone and skin. This...

EVII activation in blast crisis CML due to juxtaposition to the rare 17q22 partner region as part of a 4-way variant translocation t(9;22) (2008)

De Weer, An, Poppe, Bruce, Cauwelier, Barbara, CARLIER, A, DIERICK, J, Verhasselt, Bruno, ...

Background: Variant translocations t( 9; 22) occur in 5 to 10% of newly diagnosed CMLs and additional genetic changes are present in 60 - 80% of patients in blast crisis ( BC). Here, we report on a...

Mapping of 5q35 chromosomal rearrangements within a genomically unstable region (2008)

Buysse, Karen, CREPEL, A, Menten, Björn, Pattyn, Filip, ANTONACCI, F, VELTMAN, J, ...

Background: Recent molecular studies of breakpoints of recurrent chromosome rearrangements revealed the role of genomic architecture in their formation. In particular, segmental duplications...

EVI1 overexpression in t(3;17) positive myeloid malignancies results from juxtaposition of EVI1 to the MSI2 locus at 17q22 (2008)

De Weer, An, Speleman, Franki, Cauwelier, Barbara, Van Roy, Nadine, Yigit, Nurten, Verhasselt, Bruno, ...

Chromosomal translocations involving the EVI1 locus are a recurrent finding in myeloid leukemia and are associated with poor prognosis. In this study, we performed a detailed molecular...

EVII activation in blast crisis CML due to juxtaposition to the rare 17q22 partner region as part of a 4-way variant translocation t(9;22) (2008)

De Weer, An, Poppe, Bruce, Cauwelier, Barbara, Carlier, Andre, Dierick, Jan, Verhasselt, Bruno, ...

Background: Variant translocations t( 9; 22) occur in 5 to 10% of newly diagnosed CMLs and additional genetic changes are present in 60 - 80% of patients in blast crisis ( BC). Here, we report on a...

EVI1 overexpression in t(3;17) positive myeloid malignancies results from juxtaposition of EVI1 to the MSI2 locus at 17q22 (2008)

De Weer, An, Speleman, Franki, Cauwelier, Barbara, Van Roy, Nadine, Yigit, Nurten, Verhasselt, Bruno, ...

Chromosomal translocations involving the EVI1 locus are a recurrent finding in myeloid leukemia and are associated with poor prognosis. In this study, we performed a detailed molecular...

Copy number alterations and copy number variation in cancer: close encounters of the bad kind (2008)

Speleman, Franki, Kumps, Candy, Buysse, Karen, Poppe, Bruce, Menten, Björn, De Preter, Katleen

Recent studies have unveiled copy number variants (CNVs) as an important source of genetic variation. Many of these CNVs contain coding sequences, which have been shown to be dosage sensitive....

Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing (2008)

Van Maerken, Tom, Mestdagh, Pieter, De Clercq, Sarah, Pattyn, Filip, Yigit, Nurten, De Paepe, Anne, ...

This article describes how rt-qPCR can be implemented as a tool to monitor silencing efficiency and functional effects of RNA interference (RNAi)-mediated gene knockdown, using examples from our...

Clinical, cytogenetic and molecular characteristics of 14 T-ALL patients carrying the TCRb-HOXA rearrangement : a study of the Groupe Francophone de Cytogénétique Hématologique (GFCH). (2007)

Cauwelier, B, Cavé, H, Gervais, C, Lessard, M, Barin, C, Perot, C, ...

Recently, we and others described a new chromosomal rearrangement, that is, inv( 7)( p15q34) and t( 7; 7)( p15; q34) involving the T-cell receptor beta ( TCR beta) ( 7q34) and the HOXA gene locus (...

Duplication of the MYB oncogene in T cell acute lymphoblastic leukemia (2007)

Lahortiga, Idoya, De Keersmaecker, Kim, Van Vlierberghe, Pieter, Graux, Carlos, Cauwelier, Barbara, Lambert, Frederic, ...

We identified a duplication of the MYB oncogene in 8.4% of individuals with T cell acute lymphoblastic leukemia (T-ALL) and in five T-ALL cell lines. The duplication is associated with a threefold...

Comprehensive NF1 screening on cultured Schwann cells from neurofibromas: towards an extensive study of the somatic mutation spectrum in neurofibromatosis type 1 (2006)

Maertens, Ophélia, Brems, Hilde, Vandesompele, Jo, De Raedt, Thomas, Heyns, Ine, Rosenbaum, Thorsten, ...

Neurofibromatosis type 1 (NF1) is mainly characterized by the occurrence of benign peripheral nerve sheath tumors or neurofibromas. Thorough investigation of the somatic mutation spectrum has thus...