Methicillin-resistant Staphylococcus aureus strain USA300: origin and epidemiology (2009)
Tenover, Fred C., Goering, Richard V.
Methicillin-resistant Staphylococcus aureus (MRSA) PFGE strain type USA300 (multilocus sequence type 8, clonal complex 8, staphylococcal cassette chromosome mec type IV) was first reported in the USA...
Endimiani, Andrea, Hujer, Andrea M., Perez, Federico, Bethel, Christopher R., Hujer, Kristine M., Kroeger, Jennifer, ...
Background The emergence of blaKPC-containing Klebsiella pneumoniae (KPC-Kp) isolates is attracting significant attention. Outbreaks in the Eastern USA have created serious treatment and infection...
Endimiani, Andrea, DePasquale, John M., Forero, Sandra, Perez, Federico, Hujer, Andrea M., Roberts-Pollack, Daneshia, ...
Objectives To characterize isolates of Klebsiella pneumoniae producing KPC carbapenemase (KPC-Kp) associated with an outbreak in a long-term acute care hospital (LTACH) in South Florida. Methods...
Endimiani, Andrea, Carias, Lenore L., Hujer, Andrea M., Bethel, Christopher R., Hujer, Kristine M., Perez, Federico, ...
The presence of plasmid-mediated quinolone resistance genes [i.e., qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA] was evaluated among 42 bla(KPC)-containing Klebsiella pneumoniae isolates collected in...
Hujer, Kristine M., Hamza, Nashaat S., Hujer, Andrea M., Perez, Federico, Helfand, Marion S., Bethel, Christopher R., ...
Acinetobacter spp. are emerging as opportunistic hospital pathogens that demonstrate resistance to many classes of antibiotics. In a metropolitan hospital in Cleveland, a clinical isolate of...
Swenson, Jana M., Tenover, Fred C.
The need for new antimicrobial agents with activity against Gram-positive organisms has become increasingly important because of emerging resistance. We compared the activity of a new b-lactam...
Sewell, David L., Barry, Arthur L., Allen, Stephen D., Fuchs, Peter C., Murray, Patrick R., Tenover, Fred C.
The susceptibility of Neisseria gonorrhoeae to PD 131628 and grepafloxacin (OPC 17116) was evaluated by agar dilution and disc diffusion methods. A tentative susceptibility category for both...
Steward, Christine D., Stocker, Sheila A., Swenson, Jana M., O’Hara, Caroline M., Edwards, Jonathan R., Gaynes, Robert P., ...
Fluoroquinolone resistance appears to be increasing in many species of bacteria, particularly in those causing nosocomial infections. However, the accuracy of some antimicrobial susceptibility...
Hubert, Susannah K., Mohammed, Jasmine M., Fridkin, Scott K., Gaynes, Robert P., McGowan, John E., Tenover, Fred C.
Isolates of Staphylococcus aureus with decreased susceptibilities to glycopeptide antimicrobial agents, such as vancomycin and teicoplanin, have emerged in the United States and elsewhere....
Tenover, Fred C., Jones, Ronald N., Swenson, Jana M., Zimmer, Barbara, McAllister, Sigrid, Jorgensen, James H.
A multilaboratory study was undertaken to determine the accuracy of the current National Committee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoints for broth microdilution and disk...
Tenover, Fred C., Mohammed, M. Jasmine, Gorton, Timothy S., Dembek, Zygmunt F.
Extended-spectrum β-lactamases (ESBLs) are enzymes produced in some gram-negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. They are most common in...
Hill, Bertha C., Baker, Carolyn N., Tenover, Fred C.
Present methods of antimicrobial susceptibility testing of Bordetella pertussis are time consuming and require specialized media that are not commercially available. We tested 52 isolates of B....
Mohammed, M. Jasmine, Tenover, Fred C.
Antimicrobial resistance continues to increase worldwide among isolates of Streptococcus pneumoniae and other species of streptococci. Increasing rates of penicillin resistance, particularly in...
A 5′ Nuclease PCR (TaqMan) High-Throughput Assay for Detection of the mecA Gene in Staphylococci
Killgore, George E., Holloway, Brian, Tenover, Fred C.
In an effort to find a rapid, efficient, and reliable method of screening large numbers of bacterial isolates for specific antimicrobial resistance genes, we compared conventional PCR results to the...
Deplano, Ariane, Schuermans, Annette, Van Eldere, Johan, Witte, Wolfgang, Meugnier, Hèléne, Etienne, Jerome, ...
Rapid and efficient epidemiologic typing systems would be useful to monitor transmission of methicillin-resistant Staphylococcus aureus (MRSA) at both local and interregional levels. To evaluate the...
Tenover, Fred C., Mohammed, M. Jasmine, Stelling, John, O'Brien, Thomas, Williams, Rosamund
The accuracy of antimicrobial susceptibility data submitted by microbiology laboratories to national and international surveillance systems has been debated for a number of years. To assess the...
Lee, Wee Gyo, Jernigan, John A., Rasheed, James K., Anderson, Gregory J., Tenover, Fred C.
A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11...
Daneshvar, Maryam I., Hollis, Dannie G., Steigerwalt, Arnold G., Whitney, Anne M., Spangler, Laura, Douglas, Michael P., ...
CDC weak oxidizer group 2 (WO-2) consists of nine phenotypically similar human clinical isolates received by the Centers for Disease Control and Prevention between 1989 and 1998. Four of the isolates...
Steward, Christine D., Rasheed, J. Kamile, Hubert, Susannah K., Biddle, James W., Raney, Patti M., Anderson, Gregory J., ...
Extended-spectrum β-lactamases (ESBLs) are enzymes found in gram-negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. In 1999, the National Committee for...
Swenson, Jana M., Spargo, Jean, Tenover, Fred C., Ferraro, Mary Jane
To define more precisely the inoculation methods to be used in the oxacillin screen test for Staphylococcus aureus, we tested agar screen plates prepared in house with 6 μg of oxacillin/ml and 4%...
Swenson, Jana M., Williams, Portia P., Killgore, George, O'Hara, Caroline Mohr, Tenover, Fred C.
Using a set of 55 Staphylococcus aureus challenge organisms, we evaluated six routine methods (broth microdilution, disk diffusion, oxacillin agar screen, MicroScan conventional panels, MicroScan...
Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis
Clark, Nancye C., Olsvik, Ørjan, Swenson, Jana M., Spiegel, Carol A., Tenover, Fred C.
Genes encoding streptomycin/spectinomycin adenylyltransferases [ANT(3")(9)] have been reported to exist in gram-negative organisms and Staphylococcus aureus. During a study of high-level...
Rasheed, J. Kamile, Anderson, Gregory J., Yigit, Hesna, Queenan, Anne Marie, Doménech-Sánchez, Antonio, Swenson, Jana M., ...
Klebsiella pneumoniae K6 (ATCC 700603), a clinical isolate, is resistant to ceftazidime and other oxyimino-β-lactams. A consistent reduction in the MICs of oxyimino-β-lactams by at least 3 twofold...
Yigit, Hesna, Queenan, Anne Marie, Anderson, Gregory J., Domenech-Sanchez, Antonio, Biddle, James W., Steward, Christine D., ...
A Klebsiella pneumoniae isolate showing moderate to high-level imipenem and meropenem resistance was investigated. The MICs of both drugs were 16 μg/ml. The β-lactamase activity against imipenem...
Tenover, Fred C., Lancaster, Michael V., Hill, Bertha C., Steward, Christine D., Stocker, Sheila A., Hancock, Gary A., ...
During the last several years a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides have been reported. We selected 12 isolates of...
Tenover, Fred C., Lancaster, Michael V., Hill, Bertha C., Steward, Christine D., Stocker, Sheila A., Hancock, Gary A., ...
Clark, Nancye C., Teixeira, Lucia M., Facklam, Richard R., Tenover, Fred C.
The VanC phenotype, as found in Enterococcus gallinarum, E. casseliflavus, and E. flavescens, is characterized by intrinsic low-level resistance to vancomycin. The nucleotide sequences of the vanC-1...
Rafferty, Mary Ellen, Baltch, Aldona L., Smith, Raymond P., Bopp, Lawrence H., Rheal, Carol, Tenover, Fred C., ...
During an outbreak of diarrhea in a general hospital in 1992, 166 Clostridium difficile isolates from 102 patients were typed by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR),...
Isolation of Enterobacter intermedium from the Gallbladder of a Patient with Cholecystitis
O’Hara, Caroline Mohr, Steward, Christine D., Wright, Joan L., Tenover, Fred C., Miller, J. Michael
We describe the isolation and identification of Enterobacter intermedium from the gallbladder of a patient with cholecystitis. There have been only four documented isolations of this organism from...
McDougal, Linda K., Tenover, Fred C., Lee, Linda N., Rasheed, J. Kamile, Patterson, Jan E., Jorgensen, James H., ...
A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization....
gyrA Mutations Associated with Fluoroquinolone Resistance in Eight Species of Enterobacteriaceae
Weigel, Linda M., Steward, Christine D., Tenover, Fred C.
Fluoroquinolone resistance (FQ-R) in clinical isolates of Enterobacteriaceae species has been reported with increasing frequency in recent years. Two mechanisms of FQ-R have been identified in...
Nicola, Federico G., McDougal, Linda K., Biddle, James W., Tenover, Fred C.
We tested 16 erythromycin-resistant clinical isolates of S. aureus, recovered from patients hospitalized in the United States from 1958 to 1969, for the presence of ermA, ermB, and ermC by using PCR....
Rasheed, J. Kamile, Anderson, Gregory J., Queenan, Anne Marie, Biddle, James W., Oliver, Antonio, Jacoby, George A., ...
TEM-71, a novel extended-spectrum β-lactamase from a Klebsiella pneumoniae clinical isolate, had an isoelectric point of 6.0 and a substrate profile showing preferential hydrolysis of cefotaxime...
Mohammed, M. Jasmine, Marston, Chung K., Popovic, Tanja, Weyant, Robbin S., Tenover, Fred C.
We determined the patterns of antimicrobial susceptibility of 65 isolates of Bacillus anthracis (50 historical and 15 recent U.S. clinical isolates) to nine antimicrobial agents using the National...
Yigit, Hesna, Anderson, Gregory J., Biddle, James W., Steward, Christine D., Rasheed, J. Kamile, Valera, Lourdes L., ...
We investigated the mechanism of imipenem resistance in Enterobacter aerogenes strain 810, a clinical isolate from the United States for which the imipenem MIC was 16 μg/ml and the meropenem MIC was...
Mechanisms of Decreased Susceptibility to Cefpodoxime in Escherichia coli
Oliver, Antonio, Weigel, Linda M., Rasheed, J. Kamile, McGowan Jr., John E., Raney, Patti, Tenover, Fred C.
Cefpodoxime is one of five antimicrobial agents recommended by the National Committee for Clinical Laboratory Standards for screening isolates of Klebsiella spp. and Escherichia coli for...
Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community
Okuma, Keiko, Iwakawa, Kozue, Turnidge, John D., Grubb, Warren B., Bell, Jan M., O'Brien, Frances G., ...
Multiple methicillin-resistant Staphylococcus aureus (MRSA) clones carrying type IV staphylococcal cassette chromosome mec were identified in the community-acquired MRSA strains of both the United...
β-Lactamase Genes of the Penicillin-Susceptible Bacillus anthracis Sterne Strain
Chen, Yahua, Succi, Janice, Tenover, Fred C., Koehler, Theresa M.
Susceptibility to penicillin and other β-lactam-containing compounds is a common trait of Bacillus anthracis. β-lactam agents, particularly penicillin, have been used worldwide to treat anthrax in...
Cell Wall Thickening Is a Common Feature of Vancomycin Resistance in Staphylococcus aureus
Cui, Longzhu, Ma, Xiaoxue, Sato, Katsuhiro, Okuma, Keiko, Tenover, Fred C., Mamizuka, Elsa M., ...
We have previously shown that a thickened cell wall is responsible for the vancomycin resistance of vancomycin-resistant Staphylococcus aureus (VRSA) (equivalent to vancomycin-intermediate S. aureus...
Steward, Christine D., Mohammed, Jasmine M., Swenson, Jana M., Stocker, Sheila A., Williams, Portia P., Gaynes, Robert P., ...
From January 1996 to May 1999, Project ICARE (Intensive Care Antimicrobial Resistance Epidemiology) received 448 nonduplicate clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa that...
Chaitram, Jasmine M., Jevitt, Laura A., Lary, Sara, Tenover, Fred C.
A total of 150 laboratories in 33 countries that followed the NCCLS testing procedures participated in the World Health Organization's External Quality Assurance System for Antimicrobial...
Duck, William M., Steward, Christine D., Banerjee, Shailen N., Tenover, Fred C.
Computer-assisted analysis of pulsed-field gel electrophoresis (PFGE) libraries can facilitate comparisons of fragment patterns present on multiple gels. We evaluated the ability of the Advanced...
Tenover, Fred C., Raney, Patti M., Williams, Portia P., Rasheed, J. Kamile, Biddle, James W., Oliver, Antonio, ...
To determine whether confirmatory tests for extended-spectrum β-lactamase (ESBL) production in Escherichia coli are necessary, we selected 131 E. coli isolates that met the National Committee for...
Comparison of PCR Assay to Culture for Surveillance Detection of Vancomycin-Resistant Enterococci
Paule, Suzanne M., Trick, William E., Tenover, Fred C., Lankford, Mary, Cunningham, Susan, Stosor, Valentina, ...
Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46;...
McDougal, Linda K., Steward, Christine D., Killgore, George E., Chaitram, Jasmine M., McAllister, Sigrid K., Tenover, Fred C.
Oxacillin-resistant Staphylococcus aureus (ORSA) is a virulent pathogen responsible for both health care-associated and community onset disease. We used SmaI-digested genomic DNA separated by...
Carbapenem-Resistant Strain of Klebsiella oxytoca Harboring Carbapenem-Hydrolyzing β-Lactamase KPC-2
Yigit, Hesna, Queenan, Anne Marie, Rasheed, J. Kamile, Biddle, James W., Domenech-Sanchez, Antonio, Alberti, Sebastian, ...
We investigated a Klebsiella oxytoca isolate demonstrating resistance to imipenem, meropenem, extended-spectrum cephalosporins, and aztreonam. The MICs of both imipenem and meropenem were 32 μg/ml....
Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania
Tenover, Fred C., Weigel, Linda M., Appelbaum, Peter C., McDougal, Linda K., Chaitram, Jasmine, McAllister, Sigrid, ...
A vancomycin-resistant Staphylococcus aureus (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and...
Schwaber, Mitchell J., Raney, Patti M., Rasheed, J. Kamile, Biddle, James W., Williams, Portia, McGowan, John E., ...
NCCLS screening and confirmation methods for detecting extended-spectrum β-lactamases (ESBLs) apply only to Escherichia coli and Klebsiella spp., yet ESBLs have been found in other members of the...
Kaye, Keith S., Gold, Howard S., Schwaber, Mitchell J., Venkataraman, Lata, Qi, Youlin, De Girolami, Paola C., ...
This study analyzed the enzymatic basis and molecular epidemiology of amoxicillin-clavulanate-resistant Escherichia coli isolated by the microbiology laboratory of a United States tertiary care...
Diekema, Daniel J., Lee, Kathleen, Raney, Patti, Herwaldt, Loreen A., Doern, Gary V., Tenover, Fred C.
Accurate antimicrobial susceptibility testing (AST) and appropriate reporting of AST results for pathogens isolated from blood cultures are critical functions of the microbiology laboratory. We...
Swenson, Jana M., Killgore, George E., Tenover, Fred C.
Although both broth microdilution (BMD) and disk diffusion (DD) are listed by NCCLS as acceptable methods for testing Acinetobacter spp. for antimicrobial susceptibility, few studies have compared...
β-Lactamase Gene Expression in a Penicillin-Resistant Bacillus anthracis Strain
Chen, Yahua, Tenover, Fred C., Koehler, Theresa M.
Expression of the bla1 and bla2 genes in an archetypal Bacillus anthracis strain is insufficient for penicillin resistance. In a penicillin-resistant clinical isolate, both genes are highly...
Clark, Nancye C., Weigel, Linda M., Patel, Jean B., Tenover, Fred C.
In 2002, the first two clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA) containing vanA were recovered in Michigan and Pennsylvania. Tn1546, a mobile genetic element that...
Welsh, Kerry J., Barlow, Miriam, Tenover, Fred C., Biddle, James W., Rasheed, J. Kamile, Clark, Leigh Ann, ...
We applied in vitro evolution to an Escherichia coli strain containing blaCTX-M-2 and obtained 10 independent mutant blaCTX-M-2 alleles that confer elevated resistance to ceftazidime (MIC ≥ 32...
Steward, Christine D., Raney, Patti M., Morrell, Allison K., Williams, Portia P., McDougal, Linda K., Jevitt, Laura, ...
Disk diffusion and broth microdilution (BMD) were used to perform clindamycin (CLI) induction testing on 128 selected nonduplicate isolates of Staphylococcus aureus. Disk diffusion testing involved...
Zelazny, Adrian M., Ferraro, Mary Jane, Glennen, Anita, Hindler, Janet F., Mann, Linda M., Munro, Susan, ...
A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four...
Swenson, Jana M., Tenover, Fred C.
The cefoxitin disk diffusion (DD) test for predicting mecA-mediated oxacillin resistance in staphylococci was assessed during a three-phase study. In phase 1, one laboratory tested 62 and 53 strains...
Tenover, Fred C., McDougal, Linda K., Goering, Richard V., Killgore, George, Projan, Steven J., Patel, Jean B., ...
A highly stable strain of Staphylococcus aureus with a pulsed-field gel electrophoresis type of USA300 and multilocus sequence type 8 has been isolated from patients residing in diverse geographic...
Hujer, Kristine M., Hamza, Nashaat S., Hujer, Andrea M., Perez, Federico, Helfand, Marion S., Bethel, Christopher R., ...
Acinetobacter spp. are emerging as opportunistic hospital pathogens that demonstrate resistance to many classes of antibiotics. In a metropolitan hospital in Cleveland, a clinical isolate of...
Jorgensen, James H., Crawford, Sharon A., Fulcher, Letitia C., Glennen, Anita, Harrington, Susan M., Swenson, Jana, ...
In 2005, the Clinical and Laboratory Standards Institute published MIC interpretive criteria for 13 antimicrobial agents used for either therapy or prophylaxis of Neisseria meningitidis infections....
Steward, Christine D., Stocker, Sheila A., Swenson, Jana M., O’Hara, Caroline M., Edwards, Jonathan R., Gaynes, Robert P., ...
Fluoroquinolone resistance appears to be increasing in many species of bacteria, particularly in those causing nosocomial infections. However, the accuracy of some antimicrobial susceptibility...
Hubert, Susannah K., Mohammed, Jasmine M., Fridkin, Scott K., Gaynes, Robert P., McGowan, John E., Tenover, Fred C.
Isolates of Staphylococcus aureus with decreased susceptibilities to glycopeptide antimicrobial agents, such as vancomycin and teicoplanin, have emerged in the United States and elsewhere....
Tenover, Fred C., Jones, Ronald N., Swenson, Jana M., Zimmer, Barbara, McAllister, Sigrid, Jorgensen, James H.
A multilaboratory study was undertaken to determine the accuracy of the current National Committee for Clinical Laboratory Standards (NCCLS) oxacillin breakpoints for broth microdilution and disk...
Tenover, Fred C., Mohammed, M. Jasmine, Gorton, Timothy S., Dembek, Zygmunt F.
Extended-spectrum β-lactamases (ESBLs) are enzymes produced in some gram-negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. They are most common in...
Hill, Bertha C., Baker, Carolyn N., Tenover, Fred C.
Present methods of antimicrobial susceptibility testing of Bordetella pertussis are time consuming and require specialized media that are not commercially available. We tested 52 isolates of B....
Mohammed, M. Jasmine, Tenover, Fred C.
Antimicrobial resistance continues to increase worldwide among isolates of Streptococcus pneumoniae and other species of streptococci. Increasing rates of penicillin resistance, particularly in...
A 5′ Nuclease PCR (TaqMan) High-Throughput Assay for Detection of the mecA Gene in Staphylococci
Killgore, George E., Holloway, Brian, Tenover, Fred C.
In an effort to find a rapid, efficient, and reliable method of screening large numbers of bacterial isolates for specific antimicrobial resistance genes, we compared conventional PCR results to the...
Deplano, Ariane, Schuermans, Annette, Van Eldere, Johan, Witte, Wolfgang, Meugnier, Hèléne, Etienne, Jerome, ...
Rapid and efficient epidemiologic typing systems would be useful to monitor transmission of methicillin-resistant Staphylococcus aureus (MRSA) at both local and interregional levels. To evaluate the...
Tenover, Fred C., Mohammed, M. Jasmine, Stelling, John, O'Brien, Thomas, Williams, Rosamund
The accuracy of antimicrobial susceptibility data submitted by microbiology laboratories to national and international surveillance systems has been debated for a number of years. To assess the...
Lee, Wee Gyo, Jernigan, John A., Rasheed, James K., Anderson, Gregory J., Tenover, Fred C.
A total of 25 isolates of vanB-containing Enterococcus faecium were recovered from patients in a single Korean hospital over a 20-month period. There were two distinct vanB2 patterns among the 11...
Daneshvar, Maryam I., Hollis, Dannie G., Steigerwalt, Arnold G., Whitney, Anne M., Spangler, Laura, Douglas, Michael P., ...
CDC weak oxidizer group 2 (WO-2) consists of nine phenotypically similar human clinical isolates received by the Centers for Disease Control and Prevention between 1989 and 1998. Four of the isolates...
Steward, Christine D., Rasheed, J. Kamile, Hubert, Susannah K., Biddle, James W., Raney, Patti M., Anderson, Gregory J., ...
Extended-spectrum β-lactamases (ESBLs) are enzymes found in gram-negative bacilli that mediate resistance to extended-spectrum cephalosporins and aztreonam. In 1999, the National Committee for...
Swenson, Jana M., Spargo, Jean, Tenover, Fred C., Ferraro, Mary Jane
To define more precisely the inoculation methods to be used in the oxacillin screen test for Staphylococcus aureus, we tested agar screen plates prepared in house with 6 μg of oxacillin/ml and 4%...
Swenson, Jana M., Williams, Portia P., Killgore, George, O'Hara, Caroline Mohr, Tenover, Fred C.
Using a set of 55 Staphylococcus aureus challenge organisms, we evaluated six routine methods (broth microdilution, disk diffusion, oxacillin agar screen, MicroScan conventional panels, MicroScan...
Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene (aadA) in Enterococcus faecalis
Clark, Nancye C., Olsvik, Ørjan, Swenson, Jana M., Spiegel, Carol A., Tenover, Fred C.
Genes encoding streptomycin/spectinomycin adenylyltransferases [ANT(3")(9)] have been reported to exist in gram-negative organisms and Staphylococcus aureus. During a study of high-level...
Rasheed, J. Kamile, Anderson, Gregory J., Yigit, Hesna, Queenan, Anne Marie, Doménech-Sánchez, Antonio, Swenson, Jana M., ...
Klebsiella pneumoniae K6 (ATCC 700603), a clinical isolate, is resistant to ceftazidime and other oxyimino-β-lactams. A consistent reduction in the MICs of oxyimino-β-lactams by at least 3 twofold...
Yigit, Hesna, Queenan, Anne Marie, Anderson, Gregory J., Domenech-Sanchez, Antonio, Biddle, James W., Steward, Christine D., ...
A Klebsiella pneumoniae isolate showing moderate to high-level imipenem and meropenem resistance was investigated. The MICs of both drugs were 16 μg/ml. The β-lactamase activity against imipenem...
Tenover, Fred C., Lancaster, Michael V., Hill, Bertha C., Steward, Christine D., Stocker, Sheila A., Hancock, Gary A., ...
During the last several years a series of staphylococcal isolates that demonstrated reduced susceptibility to vancomycin or other glycopeptides have been reported. We selected 12 isolates of...
Tenover, Fred C., Lancaster, Michael V., Hill, Bertha C., Steward, Christine D., Stocker, Sheila A., Hancock, Gary A., ...
Clark, Nancye C., Teixeira, Lucia M., Facklam, Richard R., Tenover, Fred C.
The VanC phenotype, as found in Enterococcus gallinarum, E. casseliflavus, and E. flavescens, is characterized by intrinsic low-level resistance to vancomycin. The nucleotide sequences of the vanC-1...
Rafferty, Mary Ellen, Baltch, Aldona L., Smith, Raymond P., Bopp, Lawrence H., Rheal, Carol, Tenover, Fred C., ...
During an outbreak of diarrhea in a general hospital in 1992, 166 Clostridium difficile isolates from 102 patients were typed by restriction enzyme analysis (REA), arbitrarily primed PCR (AP-PCR),...
Isolation of Enterobacter intermedium from the Gallbladder of a Patient with Cholecystitis
O’Hara, Caroline Mohr, Steward, Christine D., Wright, Joan L., Tenover, Fred C., Miller, J. Michael
We describe the isolation and identification of Enterobacter intermedium from the gallbladder of a patient with cholecystitis. There have been only four documented isolations of this organism from...
McDougal, Linda K., Tenover, Fred C., Lee, Linda N., Rasheed, J. Kamile, Patterson, Jan E., Jorgensen, James H., ...
A series of macrolide-lincosamide-streptogramin B (MLS)-resistant pneumococcal isolates of a variety of serotypes was examined and was found to contain Tn917-like elements by DNA-DNA hybridization....
gyrA Mutations Associated with Fluoroquinolone Resistance in Eight Species of Enterobacteriaceae
Weigel, Linda M., Steward, Christine D., Tenover, Fred C.
Fluoroquinolone resistance (FQ-R) in clinical isolates of Enterobacteriaceae species has been reported with increasing frequency in recent years. Two mechanisms of FQ-R have been identified in...
Nicola, Federico G., McDougal, Linda K., Biddle, James W., Tenover, Fred C.
We tested 16 erythromycin-resistant clinical isolates of S. aureus, recovered from patients hospitalized in the United States from 1958 to 1969, for the presence of ermA, ermB, and ermC by using PCR....
Rasheed, J. Kamile, Anderson, Gregory J., Queenan, Anne Marie, Biddle, James W., Oliver, Antonio, Jacoby, George A., ...
TEM-71, a novel extended-spectrum β-lactamase from a Klebsiella pneumoniae clinical isolate, had an isoelectric point of 6.0 and a substrate profile showing preferential hydrolysis of cefotaxime...
Mohammed, M. Jasmine, Marston, Chung K., Popovic, Tanja, Weyant, Robbin S., Tenover, Fred C.
We determined the patterns of antimicrobial susceptibility of 65 isolates of Bacillus anthracis (50 historical and 15 recent U.S. clinical isolates) to nine antimicrobial agents using the National...
Yigit, Hesna, Anderson, Gregory J., Biddle, James W., Steward, Christine D., Rasheed, J. Kamile, Valera, Lourdes L., ...
We investigated the mechanism of imipenem resistance in Enterobacter aerogenes strain 810, a clinical isolate from the United States for which the imipenem MIC was 16 μg/ml and the meropenem MIC was...
Mechanisms of Decreased Susceptibility to Cefpodoxime in Escherichia coli
Oliver, Antonio, Weigel, Linda M., Rasheed, J. Kamile, McGowan Jr., John E., Raney, Patti, Tenover, Fred C.
Cefpodoxime is one of five antimicrobial agents recommended by the National Committee for Clinical Laboratory Standards for screening isolates of Klebsiella spp. and Escherichia coli for...
Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community
Okuma, Keiko, Iwakawa, Kozue, Turnidge, John D., Grubb, Warren B., Bell, Jan M., O'Brien, Frances G., ...
Multiple methicillin-resistant Staphylococcus aureus (MRSA) clones carrying type IV staphylococcal cassette chromosome mec were identified in the community-acquired MRSA strains of both the United...
β-Lactamase Genes of the Penicillin-Susceptible Bacillus anthracis Sterne Strain
Chen, Yahua, Succi, Janice, Tenover, Fred C., Koehler, Theresa M.
Susceptibility to penicillin and other β-lactam-containing compounds is a common trait of Bacillus anthracis. β-lactam agents, particularly penicillin, have been used worldwide to treat anthrax in...
Cell Wall Thickening Is a Common Feature of Vancomycin Resistance in Staphylococcus aureus
Cui, Longzhu, Ma, Xiaoxue, Sato, Katsuhiro, Okuma, Keiko, Tenover, Fred C., Mamizuka, Elsa M., ...
We have previously shown that a thickened cell wall is responsible for the vancomycin resistance of vancomycin-resistant Staphylococcus aureus (VRSA) (equivalent to vancomycin-intermediate S. aureus...
Steward, Christine D., Mohammed, Jasmine M., Swenson, Jana M., Stocker, Sheila A., Williams, Portia P., Gaynes, Robert P., ...
From January 1996 to May 1999, Project ICARE (Intensive Care Antimicrobial Resistance Epidemiology) received 448 nonduplicate clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa that...
Chaitram, Jasmine M., Jevitt, Laura A., Lary, Sara, Tenover, Fred C.
A total of 150 laboratories in 33 countries that followed the NCCLS testing procedures participated in the World Health Organization's External Quality Assurance System for Antimicrobial...
Duck, William M., Steward, Christine D., Banerjee, Shailen N., Tenover, Fred C.
Computer-assisted analysis of pulsed-field gel electrophoresis (PFGE) libraries can facilitate comparisons of fragment patterns present on multiple gels. We evaluated the ability of the Advanced...
Tenover, Fred C., Raney, Patti M., Williams, Portia P., Rasheed, J. Kamile, Biddle, James W., Oliver, Antonio, ...
To determine whether confirmatory tests for extended-spectrum β-lactamase (ESBL) production in Escherichia coli are necessary, we selected 131 E. coli isolates that met the National Committee for...
Comparison of PCR Assay to Culture for Surveillance Detection of Vancomycin-Resistant Enterococci
Paule, Suzanne M., Trick, William E., Tenover, Fred C., Lankford, Mary, Cunningham, Susan, Stosor, Valentina, ...
Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46;...
McDougal, Linda K., Steward, Christine D., Killgore, George E., Chaitram, Jasmine M., McAllister, Sigrid K., Tenover, Fred C.
Oxacillin-resistant Staphylococcus aureus (ORSA) is a virulent pathogen responsible for both health care-associated and community onset disease. We used SmaI-digested genomic DNA separated by...
Carbapenem-Resistant Strain of Klebsiella oxytoca Harboring Carbapenem-Hydrolyzing β-Lactamase KPC-2
Yigit, Hesna, Queenan, Anne Marie, Rasheed, J. Kamile, Biddle, James W., Domenech-Sanchez, Antonio, Alberti, Sebastian, ...
We investigated a Klebsiella oxytoca isolate demonstrating resistance to imipenem, meropenem, extended-spectrum cephalosporins, and aztreonam. The MICs of both imipenem and meropenem were 32 μg/ml....
Vancomycin-Resistant Staphylococcus aureus Isolate from a Patient in Pennsylvania
Tenover, Fred C., Weigel, Linda M., Appelbaum, Peter C., McDougal, Linda K., Chaitram, Jasmine, McAllister, Sigrid, ...
A vancomycin-resistant Staphylococcus aureus (VRSA) isolate was obtained from a patient in Pennsylvania in September 2002. Species identification was confirmed by standard biochemical tests and...
Schwaber, Mitchell J., Raney, Patti M., Rasheed, J. Kamile, Biddle, James W., Williams, Portia, McGowan, John E., ...
NCCLS screening and confirmation methods for detecting extended-spectrum β-lactamases (ESBLs) apply only to Escherichia coli and Klebsiella spp., yet ESBLs have been found in other members of the...
Kaye, Keith S., Gold, Howard S., Schwaber, Mitchell J., Venkataraman, Lata, Qi, Youlin, De Girolami, Paola C., ...
This study analyzed the enzymatic basis and molecular epidemiology of amoxicillin-clavulanate-resistant Escherichia coli isolated by the microbiology laboratory of a United States tertiary care...
Diekema, Daniel J., Lee, Kathleen, Raney, Patti, Herwaldt, Loreen A., Doern, Gary V., Tenover, Fred C.
Accurate antimicrobial susceptibility testing (AST) and appropriate reporting of AST results for pathogens isolated from blood cultures are critical functions of the microbiology laboratory. We...
Swenson, Jana M., Killgore, George E., Tenover, Fred C.
Although both broth microdilution (BMD) and disk diffusion (DD) are listed by NCCLS as acceptable methods for testing Acinetobacter spp. for antimicrobial susceptibility, few studies have compared...
β-Lactamase Gene Expression in a Penicillin-Resistant Bacillus anthracis Strain
Chen, Yahua, Tenover, Fred C., Koehler, Theresa M.
Expression of the bla1 and bla2 genes in an archetypal Bacillus anthracis strain is insufficient for penicillin resistance. In a penicillin-resistant clinical isolate, both genes are highly...
Clark, Nancye C., Weigel, Linda M., Patel, Jean B., Tenover, Fred C.
In 2002, the first two clinical isolates of vancomycin-resistant Staphylococcus aureus (VRSA) containing vanA were recovered in Michigan and Pennsylvania. Tn1546, a mobile genetic element that...
Welsh, Kerry J., Barlow, Miriam, Tenover, Fred C., Biddle, James W., Rasheed, J. Kamile, Clark, Leigh Ann, ...
We applied in vitro evolution to an Escherichia coli strain containing blaCTX-M-2 and obtained 10 independent mutant blaCTX-M-2 alleles that confer elevated resistance to ceftazidime (MIC ≥ 32...
Steward, Christine D., Raney, Patti M., Morrell, Allison K., Williams, Portia P., McDougal, Linda K., Jevitt, Laura, ...
Disk diffusion and broth microdilution (BMD) were used to perform clindamycin (CLI) induction testing on 128 selected nonduplicate isolates of Staphylococcus aureus. Disk diffusion testing involved...
Zelazny, Adrian M., Ferraro, Mary Jane, Glennen, Anita, Hindler, Janet F., Mann, Linda M., Munro, Susan, ...
A nine-laboratory collaborative study was conducted to select positive and negative quality assessment control strains for the detection of inducible clindamycin resistance in staphylococci. Four...
Hujer, Kristine M., Hamza, Nashaat S., Hujer, Andrea M., Perez, Federico, Helfand, Marion S., Bethel, Christopher R., ...
Acinetobacter spp. are emerging as opportunistic hospital pathogens that demonstrate resistance to many classes of antibiotics. In a metropolitan hospital in Cleveland, a clinical isolate of...
Swenson, Jana M., Tenover, Fred C.
The cefoxitin disk diffusion (DD) test for predicting mecA-mediated oxacillin resistance in staphylococci was assessed during a three-phase study. In phase 1, one laboratory tested 62 and 53 strains...
Tenover, Fred C., McDougal, Linda K., Goering, Richard V., Killgore, George, Projan, Steven J., Patel, Jean B., ...
A highly stable strain of Staphylococcus aureus with a pulsed-field gel electrophoresis type of USA300 and multilocus sequence type 8 has been isolated from patients residing in diverse geographic...
Jorgensen, James H., Crawford, Sharon A., Fulcher, Letitia C., Glennen, Anita, Harrington, Susan M., Swenson, Jana, ...
In 2005, the Clinical and Laboratory Standards Institute published MIC interpretive criteria for 13 antimicrobial agents used for either therapy or prophylaxis of Neisseria meningitidis infections....
Jevitt, Laura A., Thorne, Grace M., Traczewski, Maria M., Jones, Ronald N., McGowan, John E., Tenover, Fred C., ...
Daptomycin is a novel cyclic lipopeptide that is approved by the U.S. Food and Drug Administration for the treatment of complicated skin and skin structure infections associated with Staphylococcus...
Bonnstetter, Kristin K., Wolter, Daniel J., Tenover, Fred C., McDougal, Linda K., Goering, Richard V.
Recent reports have noted a discernible increase in the number of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections in patients without traditional risk factors....
Stone, Nimalie D., O'Hara, Caroline M., Williams, Portia P., McGowan, John E., Tenover, Fred C.
We compared the antimicrobial susceptibility testing results generated by disk diffusion and the VITEK 2 automated system with the results of the Clinical and Laboratory Standards Institute (CLSI)...
Goering, Richard V., McDougal, Linda K., Fosheim, Greg E., Bonnstetter, Kristin K., Wolter, Daniel J., Tenover, Fred C.
We tested 214 Staphylococcus aureus isolates for the arcA locus of the arginine catabolic mobile element (ACME). All USA300 SCCmec IVa isolates, but no isolates containing other SCCmec subtypes, were...
Tenover, Fred C., Vaughn, Rebekah R., McDougal, Linda K., Fosheim, Gregory E., McGowan, John E.
Our objective was to determine if a multiple-locus variable-number tandem-repeat assay (MLVA) for Staphylococcus aureus could predict pulsed-field gel electrophoresis (PFGE) types (i.e., USA types),...
Counts, Jon M., Astles, J. Rex, Tenover, Fred C., Hindler, Janet
Laboratory practice in the preanalytical phase of antimicrobial susceptibility testing (AST) was evaluated in 102 hospital, reference, physician office-clinic, and public health laboratories in...
Tenover, Fred C., Williams, Portia P., Stocker, Sheila, Thompson, Angela, Clark, Leigh Ann, Limbago, Brandi, ...
A challenge panel of enterococci (n = 50) and staphylococci (n = 50), including 17 and 15 isolates that were nonsusceptible to linezolid, respectively, were tested with the Clinical and Laboratory...
Melles, Damian C., Tenover, Fred C., Kuehnert, Matthew J., Witsenboer, Hanneke, Peeters, Justine K., Verbrugh, Henri A., ...
To understand Staphylococcus aureus nasal carriage and its relationship with subsequent disease, insight into the natural (nonclinical) bacterial population structure is essential. This study...
Yigit, Hesna, Queenan, Anne Marie, Anderson, Gregory J., Domenech-Sanchez, Antonio, Biddle, James W., Steward, Christine D., ...
Kennedy, Adam D., Otto, Michael, Braughton, Kevin R., Whitney, Adeline R., Chen, Liang, Mathema, Barun, ...
Emerging and re-emerging infectious diseases, especially those caused by drug-resistant bacteria, are a major problem worldwide. Community-associated methicillin-resistant Staphylococcus aureus...
Endimiani, Andrea, Carias, Lenore L., Hujer, Andrea M., Bethel, Christopher R., Hujer, Kristine M., Perez, Federico, ...
The presence of plasmid-mediated quinolone resistance genes [i.e., qnrA, qnrB, qnrS, aac(6′)-Ib-cr, and qepA] was evaluated among 42 blaKPC-containing Klebsiella pneumoniae isolates collected in...
High Rate of Mobilization for blaCTX-Ms
Barlow, Miriam, Reik, Rebecca A., Jacobs, Stephen D., Medina, Mónica, Meyer, Matthew P., McGowan, John E., ...
The blaCTX-Ms have been mobilized to plasmids more frequently than other class A β-lactamases.
Tenover, Fred C., McAllister, Sigrid, Fosheim, Gregory, McDougal, Linda K., Carey, Roberta B., Limbago, Brandi, ...
This study characterizes methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant S. aureus (MRSA) isolates recovered from nasal cultures of noninstitutionalized individuals in...
Tenover, Fred C., Emery, Shannon L., Spiegel, Carol A., Bradford, Patricia A., Eells, Samantha, Endimiani, Andrea, ...
The goal of this study was to determine if the interpretations of extended-spectrum and advanced-spectrum cephalosporins (ESCs and ASCs, respectively) for isolates of Enterobacteriaceae would be...
Investigation of Bioterrorism-Related Anthrax, United States, 2001: Epidemiologic Findings
Jernigan, Daniel B., Raghunathan, Pratima L., Bell, Beth P., Brechner, Ross, Bresnitz, Eddy A., Butler, Jay C., ...
In October 2001, the first inhalational anthrax case in the United States since 1976 was identified in a media company worker in Florida. A national investigation was initiated to identify additional...