Arstila, T., Jacoby, G. A., Huovinen, P.
The X-PRESS, osmotic shock, chloroform treatment, lysozyme treatment and ultrasonic disruption methods to release five different plasmid-mediated β-lactamases from Escherichia coli and one...
A functional classification scheme for beta-lactamases and its correlation with molecular structure.
Martínez-Martínez, L, Hernández-Allés, S, Albertí, S, Tomás, J M, Benedi, V J, Jacoby, G A
Four Klebsiella pneumoniae isolates (LB1, LB2, LB3, and LB4) with increased antimicrobial resistance were obtained from the same patient. The four isolates were indistinguishable in biotype, plasmid...
Seven extended-spectrum beta-lactamases related to TEM and four enzymes derived from SHV-1 were transferred to a common Escherichia coli host so that the activity of a variety of beta-lactams could...
Rice, L B, Willey, S H, Papanicolaou, G A, Medeiros, A A, Eliopoulos, G M, Moellering, R C, ...
During a 4-month period in late 1988, we isolated ceftazidime-resistant strains of Klebsiella pneumoniae and other members of the family Enterobacteriaceae from 29 patients at a chronic-care facility...
Papanicolaou, G A, Medeiros, A A, Jacoby, G A
Klebsiella pneumoniae isolates from 11 patients at the Miriam Hospital were identified as resistant to cefoxitin and ceftibuten as well as to aztreonam, cefotaxime, and ceftazidime. Resistance could...
Jacoby, G A, Blaser, M J, Santanam, P, Hächler, H, Kayser, F H, Hare, R S, ...
Following the use of amikacin as the principal aminoglycoside at a Denver hospital, amikacin resistance appeared first in Pseudomonas aeruginosa and then in Escherichia coli, Klebsiella pneumoniae,...
Sequence of PSE-2 beta-lactamase.
Huovinen, P, Huovinen, S, Jacoby, G A
The nucleotide sequence of PSE-2 beta-lactamase, an enzyme that readily hydrolyzes both carbenicillin and oxacillin, has been determined. The deduced sequence of 266 amino acids contained 93 residues...
Detection of plasmid-mediated beta-lactamases with DNA probes.
Huovinen, S, Huovinén, P, Jacoby, G A
beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical...
Pseudomonas cepacia susceptibility to sulbactam.
For 25 of 32 Pseudomonas cepacia isolates, predominantly from sputum of adult patients, agar dilution MICs of sulbactam were 2.5 micrograms/ml, and for only one was the MIC more than 80...
beta-Lactamases and beta-lactam resistance in Escherichia coli.
Escherichia coli strains determining 17 different plasmid-determined beta-lactamases were tested for resistance to new broad-spectrum beta-lactam antibiotics. Several beta-lactamases demonstrated...
An animal source for the ROB-1 beta-lactamase of Haemophilus influenzae type b.
Medeiros, A A, Levesque, R, Jacoby, G A
The most common cause of ampicillin resistance in Haemophilus influenzae type b is production of TEM-1 beta-lactamase; however, a novel enzyme with a similar substrate profile but a quite different...
Hedges, R W, Medeiros, A A, Cohenford, M, Jacoby, G A
A novel carbenicillin-hydrolyzing beta-lactamase has been discovered in a blood isolate of Aeromonas hydrophila. The enzyme resembles plasmid-determined carbenicillinases in substrate profile but...
Five novel plasmid-determined beta-lactamases.
Medeiros, A A, Cohenford, M, Jacoby, G A
Five novel plasmid-determined beta-lactamases named TLE-1, OXA-4, OXA-5, OXA-6, and OXA-7 were detected in ampicillin-resistant isolates of Escherichia coli and carbenicillin-resistant strains of...
Philippon, A M, Paul, G C, Thabaut, A P, Jacoby, G A
CARB-4, a novel carbenicillin-hydrolyzing beta-lactamase with an isoelectric point of 4.3, was discovered in a strain of Pseudomonas aeruginosa from France. It was determined by a multiresistant...
Calderwood, S B, Gardella, A, Philippon, A M, Jacoby, G A, Moellering, R C
We investigated the effects of the combination of azlocillin with the beta-lactamase inhibitors clavulanic acid and sulbactam and with N-formimidoyl thienamycin against strains of Pseudomonas...
Cloning the gene for gentamicin resistance from Pseudomonas aeruginosa plasmid pMG35 on the high-copy-number Escherichia coli cloning vehicle pMK20 allowed detection of 6'-N-acetyltransferase...
Properties of IncP-2 plasmids of Pseudomonas spp.
Jacoby, G A, Sutton, L, Knobel, L, Mammen, P
Thirty IncP-2 R plasmids from isolates of Pseudomonas spp. of diverse geographical origins were examined for the production of resistance properties. All the plasmids determined resistance to...
Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa.
Philippon, A M, Paul, G C, Jacoby, G A
The properties of PSE-2 beta-lactamase have been examined by using two new PSE-2-producing plasmids, pMG33 and pMG74, as well as plasmid R151, found in Pseudomonas aeruginosa. PSE-2 beta-lactamase...
Sequences of MGH-1, YOU-1, and YOU-2 extended-spectrum beta-lactamase genes.
Rice, L B, Marshall, S H, Carias, L L, Sutton, L, Jacoby, G A
Genes for MGH-1, YOU-1, and YOU-2 extended-spectrum beta-lactamases have been cloned and sequenced. The gene for MGH-1 has the sequence of blaTEM-10, YOU-2 has that of blaTEM-12, and YOU-1 has that...
Spread of a "Pseudomonas-specific" beta-lactamase to plasmids of enterobacteria.
Medeiros, A A, Hedges, R W, Jacoby, G A
Eleven isolates including Escherichia coli, Salmonella enteritidis, and Shigella sonnei, obtained in Brazil, Hong Kong, Indonesia, Thailand, and the United States, were found to produce...
An explanation for the apparent host specificity of Pseudomonas plasmid R91 expression.
Jacoby, G A, Weiss, R, Korfhagen, T R, Krishnapillai, V, Jacob, A E, Hedges, R W
Pseudomonas aeruginosa strain 9169 has been reported to contain a plasmid that expresses resistance to carbenicillin (Cb), kanamycin (Km), and tetracycline (Tc) in Escherichia coli but resistance...
Forty clinical isolates of Escherichia coli and 141 isolates of Klebsiella pneumoniae that either transferred ceftazidime resistance or showed sulbactam enhancement of oxyimino-beta-lactam...
Recombination between plasmids of incompatibility groups P-1 and P-2.
Jacoby, G A, Jacob, A E, Hedges, R W
R plasmids of incompatibility group P-2 are readily transmissible between Pseudomonas strains, but not to Escherichia coli or other enterobacteria, whereas those of group P-1 have a broad host range....
Plasmid-determined resistance to tellurium compounds.
Transferable plasmids in gram-negative bacteria that confer resistance to potassium tellurite or tellurate were found. This re-istance was distinct from resistance to mercury, silver, or arsenic...
Properties of plasmids responsible for production of extended-spectrum beta-lactamases.
The extended-spectrum beta-lactamases are believed to arise by mutations which alter the configuration around the active site of TEM- and SHV-type enzymes so as to increase their efficiency with...
Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis.
Rice, L B, Eliopoulos, G M, Wennersten, C, Goldmann, D, Jacoby, G A, Moellering, R C
We have analyzed four distinct strains of multiply resistant, beta-lactamase-producing enterococci isolated during an outbreak of colonization with these strains on an infant-toddler surgical ward at...
Katsanis, G P, Spargo, J, Ferraro, M J, Sutton, L, Jacoby, G A
Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for...
Epidemiology of Conjugative Plasmid-Mediated AmpC β-Lactamases in the United States
Alvarez, M., Tran, J. H., Chow, N., Jacoby, G. A.
A sample of 752 resistant Klebsiella pneumoniae, Klebsiella oxytoca, and Escherichia coli strains from 70 sites in 25 U.S. states and the District of Columbia was examined for transmissibility of...
Falkiner, F R, Keane, C T, Dalton, M, Clancy, M T, Jacoby, G A
An outbreak of gentamicin- and tobramycin-resistant Pseudomonas aeruginosa infection occurred in a surgical ward over a three-month period. Resistant Ps. aeruginosa strains with the same serological,...
1. The first five enzymes involved in the degradation of mandelate in Pseudomonas fluorescens have been examined. 2. Induction is not significantly affected by glucose. 3. The first three enzymes...
Broader Distribution of Plasmid-Mediated Quinolone Resistance in the United States
Robicsek, A., Sahm, D. F., Strahilevitz, J., Jacoby, G. A., Hooper, D. C.
The plasmid-encoded quinolone resistance gene qnrA confers low-level quinolone resistance, facilitating selection of higher-level resistance. Epidemiologic surveys for qnrA were extended to isolates...
qnr Prevalence in Ceftazidime-Resistant Enterobacteriaceae Isolates from the United States
Robicsek, A., Strahilevitz, J., Sahm, D. F., Jacoby, G. A., Hooper, D. C.
We screened 313 ceftazidime-resistant Enterobacteriaceae isolates obtained in the United States from 1999 to 2004 for all three known qnr genes. A qnr gene was present in 20% of Klebsiella pneumoniae...
A functional classification scheme for beta-lactamases and its correlation with molecular structure.
Martínez-Martínez, L, Hernández-Allés, S, Albertí, S, Tomás, J M, Benedi, V J, Jacoby, G A
Four Klebsiella pneumoniae isolates (LB1, LB2, LB3, and LB4) with increased antimicrobial resistance were obtained from the same patient. The four isolates were indistinguishable in biotype, plasmid...
Seven extended-spectrum beta-lactamases related to TEM and four enzymes derived from SHV-1 were transferred to a common Escherichia coli host so that the activity of a variety of beta-lactams could...
Rice, L B, Willey, S H, Papanicolaou, G A, Medeiros, A A, Eliopoulos, G M, Moellering, R C, ...
During a 4-month period in late 1988, we isolated ceftazidime-resistant strains of Klebsiella pneumoniae and other members of the family Enterobacteriaceae from 29 patients at a chronic-care facility...
Papanicolaou, G A, Medeiros, A A, Jacoby, G A
Klebsiella pneumoniae isolates from 11 patients at the Miriam Hospital were identified as resistant to cefoxitin and ceftibuten as well as to aztreonam, cefotaxime, and ceftazidime. Resistance could...
Jacoby, G A, Blaser, M J, Santanam, P, Hächler, H, Kayser, F H, Hare, R S, ...
Following the use of amikacin as the principal aminoglycoside at a Denver hospital, amikacin resistance appeared first in Pseudomonas aeruginosa and then in Escherichia coli, Klebsiella pneumoniae,...
Sequence of PSE-2 beta-lactamase.
Huovinen, P, Huovinen, S, Jacoby, G A
The nucleotide sequence of PSE-2 beta-lactamase, an enzyme that readily hydrolyzes both carbenicillin and oxacillin, has been determined. The deduced sequence of 266 amino acids contained 93 residues...
Detection of plasmid-mediated beta-lactamases with DNA probes.
Huovinen, S, Huovinén, P, Jacoby, G A
beta-Lactamase identification by colony hybridization with 32P-labeled DNA probes for TEM-1, SHV-1, OXA-1, OXA-2, PSE-1, PSE-2, and PSE-4 was compared with isoelectric focusing in 122 clinical...
Pseudomonas cepacia susceptibility to sulbactam.
For 25 of 32 Pseudomonas cepacia isolates, predominantly from sputum of adult patients, agar dilution MICs of sulbactam were 2.5 micrograms/ml, and for only one was the MIC more than 80...
beta-Lactamases and beta-lactam resistance in Escherichia coli.
Escherichia coli strains determining 17 different plasmid-determined beta-lactamases were tested for resistance to new broad-spectrum beta-lactam antibiotics. Several beta-lactamases demonstrated...
An animal source for the ROB-1 beta-lactamase of Haemophilus influenzae type b.
Medeiros, A A, Levesque, R, Jacoby, G A
The most common cause of ampicillin resistance in Haemophilus influenzae type b is production of TEM-1 beta-lactamase; however, a novel enzyme with a similar substrate profile but a quite different...
Hedges, R W, Medeiros, A A, Cohenford, M, Jacoby, G A
A novel carbenicillin-hydrolyzing beta-lactamase has been discovered in a blood isolate of Aeromonas hydrophila. The enzyme resembles plasmid-determined carbenicillinases in substrate profile but...
Five novel plasmid-determined beta-lactamases.
Medeiros, A A, Cohenford, M, Jacoby, G A
Five novel plasmid-determined beta-lactamases named TLE-1, OXA-4, OXA-5, OXA-6, and OXA-7 were detected in ampicillin-resistant isolates of Escherichia coli and carbenicillin-resistant strains of...
Philippon, A M, Paul, G C, Thabaut, A P, Jacoby, G A
CARB-4, a novel carbenicillin-hydrolyzing beta-lactamase with an isoelectric point of 4.3, was discovered in a strain of Pseudomonas aeruginosa from France. It was determined by a multiresistant...
Calderwood, S B, Gardella, A, Philippon, A M, Jacoby, G A, Moellering, R C
We investigated the effects of the combination of azlocillin with the beta-lactamase inhibitors clavulanic acid and sulbactam and with N-formimidoyl thienamycin against strains of Pseudomonas...
Cloning the gene for gentamicin resistance from Pseudomonas aeruginosa plasmid pMG35 on the high-copy-number Escherichia coli cloning vehicle pMK20 allowed detection of 6'-N-acetyltransferase...
Properties of IncP-2 plasmids of Pseudomonas spp.
Jacoby, G A, Sutton, L, Knobel, L, Mammen, P
Thirty IncP-2 R plasmids from isolates of Pseudomonas spp. of diverse geographical origins were examined for the production of resistance properties. All the plasmids determined resistance to...
Properties of PSE-2 beta-lactamase and genetic basis for its production in Pseudomonas aeruginosa.
Philippon, A M, Paul, G C, Jacoby, G A
The properties of PSE-2 beta-lactamase have been examined by using two new PSE-2-producing plasmids, pMG33 and pMG74, as well as plasmid R151, found in Pseudomonas aeruginosa. PSE-2 beta-lactamase...
Sequences of MGH-1, YOU-1, and YOU-2 extended-spectrum beta-lactamase genes.
Rice, L B, Marshall, S H, Carias, L L, Sutton, L, Jacoby, G A
Genes for MGH-1, YOU-1, and YOU-2 extended-spectrum beta-lactamases have been cloned and sequenced. The gene for MGH-1 has the sequence of blaTEM-10, YOU-2 has that of blaTEM-12, and YOU-1 has that...
Spread of a "Pseudomonas-specific" beta-lactamase to plasmids of enterobacteria.
Medeiros, A A, Hedges, R W, Jacoby, G A
Eleven isolates including Escherichia coli, Salmonella enteritidis, and Shigella sonnei, obtained in Brazil, Hong Kong, Indonesia, Thailand, and the United States, were found to produce...
An explanation for the apparent host specificity of Pseudomonas plasmid R91 expression.
Jacoby, G A, Weiss, R, Korfhagen, T R, Krishnapillai, V, Jacob, A E, Hedges, R W
Pseudomonas aeruginosa strain 9169 has been reported to contain a plasmid that expresses resistance to carbenicillin (Cb), kanamycin (Km), and tetracycline (Tc) in Escherichia coli but resistance...
Forty clinical isolates of Escherichia coli and 141 isolates of Klebsiella pneumoniae that either transferred ceftazidime resistance or showed sulbactam enhancement of oxyimino-beta-lactam...
Recombination between plasmids of incompatibility groups P-1 and P-2.
Jacoby, G A, Jacob, A E, Hedges, R W
R plasmids of incompatibility group P-2 are readily transmissible between Pseudomonas strains, but not to Escherichia coli or other enterobacteria, whereas those of group P-1 have a broad host range....
Plasmid-determined resistance to tellurium compounds.
Transferable plasmids in gram-negative bacteria that confer resistance to potassium tellurite or tellurate were found. This re-istance was distinct from resistance to mercury, silver, or arsenic...
Properties of plasmids responsible for production of extended-spectrum beta-lactamases.
The extended-spectrum beta-lactamases are believed to arise by mutations which alter the configuration around the active site of TEM- and SHV-type enzymes so as to increase their efficiency with...
Chromosomally mediated beta-lactamase production and gentamicin resistance in Enterococcus faecalis.
Rice, L B, Eliopoulos, G M, Wennersten, C, Goldmann, D, Jacoby, G A, Moellering, R C
We have analyzed four distinct strains of multiply resistant, beta-lactamase-producing enterococci isolated during an outbreak of colonization with these strains on an infant-toddler surgical ward at...
Katsanis, G P, Spargo, J, Ferraro, M J, Sutton, L, Jacoby, G A
Plasmids encoding extended-spectrum beta-lactamases of the TEM, SHV, and AmpC families were introduced into common Escherichia coli and Klebsiella pneumoniae hosts to create a homogeneous panel for...
Epidemiology of Conjugative Plasmid-Mediated AmpC β-Lactamases in the United States
Alvarez, M., Tran, J. H., Chow, N., Jacoby, G. A.
A sample of 752 resistant Klebsiella pneumoniae, Klebsiella oxytoca, and Escherichia coli strains from 70 sites in 25 U.S. states and the District of Columbia was examined for transmissibility of...
Falkiner, F R, Keane, C T, Dalton, M, Clancy, M T, Jacoby, G A
An outbreak of gentamicin- and tobramycin-resistant Pseudomonas aeruginosa infection occurred in a surgical ward over a three-month period. Resistant Ps. aeruginosa strains with the same serological,...
Broader Distribution of Plasmid-Mediated Quinolone Resistance in the United States
Robicsek, A., Sahm, D. F., Strahilevitz, J., Jacoby, G. A., Hooper, D. C.
The plasmid-encoded quinolone resistance gene qnrA confers low-level quinolone resistance, facilitating selection of higher-level resistance. Epidemiologic surveys for qnrA were extended to isolates...
1. The first five enzymes involved in the degradation of mandelate in Pseudomonas fluorescens have been examined. 2. Induction is not significantly affected by glucose. 3. The first three enzymes...
qnr Prevalence in Ceftazidime-Resistant Enterobacteriaceae Isolates from the United States
Robicsek, A., Strahilevitz, J., Sahm, D. F., Jacoby, G. A., Hooper, D. C.
We screened 313 ceftazidime-resistant Enterobacteriaceae isolates obtained in the United States from 1999 to 2004 for all three known qnr genes. A qnr gene was present in 20% of Klebsiella pneumoniae...