Enhanced Isolation of Shigella Species by Extended Incubation of Primary Isolation Plates (2007)
Alam, Khorshed, Hosain, M. Anowar, Islam, Nazrul, Sen, Sunil Kumar, Sur, Gour Chandra, Kaisar A., Talukder, ...
Ansaruzzaman, M, Chowdhury, A, Bhuiyan, N.A, Safa, Ashrafus, Sultana, Marzia, Lucas, Marcelino, ...
US-Japan Cooperative medical Science Meeting
Research Note - Bengal: El Tor Cholera Vibrio in a New Robe (1994)
Carlos Andre Sailes, Hooman Momen, Ana Maria Coelho, G Balakrish Nair
Extended serotyping scheme for Vibrio cholerae (1994)
Shimada, Toshio, Arakawa, Eiji, Itoh, Kenichiro, Okitsu, Tadayuki, Matsushima, Akiyoshi, Asai, Yoshio, ...
Bag, Prasanta K., Nandi, Suvobroto, Bhadra, Rupak K., Ramamurthy, T., Bhattacharya, S. K., Nishibuchi, M., ...
The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced...
Development and Evaluation of a Phage Typing Scheme for Vibrio cholerae O139
Chakrabarti, A. K., Ghosh, A. N., Nair, G. Balakrish, Niyogi, S. K., Bhattacharya, S. K., Sarkar, B. L.
The scenario of cholera that existed previously changed in 1992 and 1993 with the emergence of toxigenic Vibrio cholerae O139 in India. The genesis of the new serogroup formed the impetus to search...
Nandi, Bisweswar, Nandy, Ranjan K., Mukhopadhyay, Sarmishtha, Nair, G. Balakrish, Shimada, Toshio, Ghose, Asoke C.
The distribution of genes for an outer membrane protein (OmpW) and a regulatory protein (ToxR) in Vibrio cholerae and other organisms was studied using respective primers and probes. PCR...
Garg, Pallavi, Nandy, Ranjan K., Chaudhury, Papiya, Chowdhury, Nandini Roy, De, Keya, Ramamurthy, T., ...
The toxigenic Inaba serotype of Vibrio cholerae O1 biotype El Tor reappeared in India in 1998 and 1999, almost 10 years after its last dominance in Calcutta in 1989. Extensive molecular...
Chakraborty, Subhra, Deokule, J. S., Garg, Pallavi, Bhattacharya, S. K., Nandy, R. K., Nair, G. Balakrish, ...
In Ahmedabad, a major city in the state of Gujarat, India, an outbreak of acute secretory diarrhea caused by Vibrio cholerae O1 Ogawa El Tor, V. cholerae O139, and multiple serotypes of...
Garg, Pallavi, Sinha, Sutapa, Chakraborty, Rupa, Bhattacharya, S. K., Nair, G. Balakrish, Ramamurthy, T., ...
Virulence Genes in Environmental Strains of Vibrio cholerae
Chakraborty, Soumen, Mukhopadhyay, Asish K., Bhadra, Rupak Kumar, Ghosh, Amar Nath, Mitra, Rupak, Shimada, Toshio, ...
The virulence of a pathogen is dependent on a discrete set of genetic determinants and their well-regulated expression. The ctxAB and tcpA genes are known to play a cardinal role in maintaining...
Distinctiveness of Genotypes of Helicobacter pylori in Calcutta, India
Mukhopadhyay, Asish K., Kersulyte, Dangeruta, Jeong, Jin-Yong, Datta, Simanti, Ito, Yoshiyuki, Chowdhury, Abhijit, ...
The genotypes of 78 strains of Helicobacter pylori from Calcutta, India (55 from ulcer patients and 23 from more-benign infections), were studied, with a focus on putative virulence genes and neutral...
Jeong, Jin-Yong, Mukhopadhyay, Asish K., Dailidiene, Daiva, Wang, Yipeng, Velapatiño, Billie, Gilman, Robert H., ...
Helicobacter pylori is a human-pathogenic bacterial species that is subdivided geographically, with different genotypes predominating in different parts of the world. Here we test and extend an...
Cell Vacuolation, a Manifestation of the El Tor Hemolysin of Vibrio cholerae
Mitra, Rupak, Figueroa, Paula, Mukhopadhyay, Asish K., Shimada, Toshio, Takeda, Yoshifumi, Berg, Douglas E., ...
Culture supernatants of nontoxigenic nonepidemic clinical strains of Vibrio cholerae belonging to diverse serogroups were found to induce vacuolation of nonconfluent HeLa cells. The vacuoles became...
Faruque, Shah M., Rahman, M. Mostafizur, Nair, G. Balakrish, Mekalanos, John J., Sack, David A.
Toxigenic Vibrio cholerae strains are lysogens of CTXΦ, a filamentous bacteriophage which encodes cholera toxin (CT). Following infection of recipient V. cholerae cells by CTXΦ, the phage genome...
Mukhopadhyay, Asish K., Chakraborty, Soumen, Takeda, Yoshifumi, Nair, G. Balakrish, Berg, Douglas E.
Environmental isolates of Vibrio cholerae of eight randomly amplified polymorphic DNA (RAPD) fingerprint types from Calcutta, India, that were unusual in containing toxin-coregulated pilus or cholera...
Sharma, Charu, Thungapathra, M., Ghosh, A., Mukhopadhyay, Asish K., Basu, Arnab, Mitra, Rupak, ...
There was an inexplicable upsurge in the incidence of non-O1, non-O139 Vibrio cholerae among hospitalized patients admitted to the Infectious Diseases Hospital, Calcutta, India, between February and...
Sharma, Charu, Ghosh, Amit, Dalsgaard, A., Forslund, Anita, Ghosh, R. K., Bhattacharya, S. K., ...
We present molecular evidence that a distinct genotype of Vibrio cholerae O1 which appeared in Calcutta, India, in September 1993 and which is characterized by a unique ribotype that is not found in...
Molecular Epidemiology of Reemergent Vibrio cholerae O139 Bengal in India
Mukhopadhyay, Asish Kumar, Basu, Arnab, Garg, Pallavi, Bag, Prasanta K., Ghosh, Amit, Bhattacharya, S. K., ...
We report the prevalence of the O139 serogroup in Calcutta, India, after its reemergence in August 1996 and the spread of the reemerged clone to other parts of the country by using previously...
Rapid Diagnosis of Cholera Caused by Vibrio cholerae O139
Chaicumpa, Wanpen, Srimanote, Potjanee, Sakolvaree, Yuwaporn, Kalampaheti, Thareerat, Chongsa-Nguan, Manas, Tapchaisri, Pramuan, ...
Hybridomas secreting specific monoclonal antibodies (MAbs) to Vibrio cholerae serogroup O139 were produced. Six monoclones (hybridomas) secreting MAbs specific only to lipopolysaccharide of V....
Pal, Sangita, Datta, Archana, Nair, G. Balakrish, Guhathakurta, Bhakti
Two hybrid clones producing monoclonal antibodies (MAbs) raised against the purified enterotoxic hemolysin-phospholipase C (HlyPC) bifunctional molecule of a Vibrio cholerae O139 strain were used to...
Bhuiyan, N. A., Ansaruzzaman, M., Kamruzzaman, M., Alam, Khorshed, Chowdhury, N. R., Nishibuchi, M., ...
Sixty-six strains of Vibrio parahaemolyticus belonging to 14 serotypes were isolated from hospitalized patients in Dhaka, Bangladesh, from January 1998 to December 2000. Among these, 48 strains...
Chattopadhyay, Santanu, Datta, Simanti, Chowdhury, Abhijit, Chowdhury, Sujit, Mukhopadhyay, Asish K., Rajendran, K., ...
We compared putative molecular markers of virulence (vacA, cagA, and iceA) of Helicobacter pylori strains isolated from 52 adult duodenal ulcer patients from West Bengal, India, with those of H....
Escalating Association of Vibrio cholerae O139 with Cholera Outbreaks in India
Sinha, Sutapa, Chakraborty, Rupa, De, Keya, Khan, Asis, Datta, Simanti, Ramamurthy, T., ...
Between December 1999 and December 2000, teams from the National Institute of Cholera and Enteric Diseases, Calcutta, India, examined eight outbreaks of cholera, which occurred in different parts of...
Faruque, Shah M., Khan, Rasel, Kamruzzaman, M., Yamasaki, Shinji, Ahmad, Q. Shafi, Azim, Tasnim, ...
Bacillary dysentery caused by Shigella species is a public health problem in developing countries including Bangladesh. Although, shigellae-contaminated food and drinks are often the source of the...
Baranwal, Somesh, Dey, Keya, Ramamurthy, T., Nair, G. Balakrish, Kundu, Manikuntala
Quinolones are among the drugs of choice in the management of cholera caused by Vibrio cholerae. In this study, we demonstrate that, in addition to mutations detected in the target genes gyrA and...
Faruque, Shah M., Kamruzzaman, M., Nandi, Ranjan K., Ghosh, A. N., Nair, G. Balakrish, ...
In toxigenic Vibrio cholerae, cholera toxin is encoded by the CTX prophage, which consists of a core region carrying ctxAB genes and genes required for CTXΦ morphogenesis, and an RS2 region encoding...
Sarkar, Amit, Nandy, Ranjan K., Nair, G. Balakrish, Ghose, Asoke C.
A non-O1 non-O139 Vibrio cholerae strain, 10259, belonging to the serogroup O53 was shown to harbor genes related to the vibrio pathogenicity island (VPI) and a cholera toxin (CT) genetic element...
Nair, G. Balakrish, Faruque, Shah M., Bhuiyan, N. A., Kamruzzaman, M., Siddique, A. K., Sack, David A.
The sixth pandemic of cholera and, presumably, the earlier pandemics were caused by the classical biotype of Vibrio cholerae O1, which was progressively replaced by the El Tor biotype representing...
Khan, Asis, Das, S. C., Ramamurthy, T., Sikdar, A., Khanam, J., Yamasaki, S., ...
Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin-producing Escherichia coli (STEC) non-O157 strains isolated from human stool samples, cow stool samples, and beef samples...
Karasawa, Tadahiro, Ito, Hideaki, Tsukamoto, Teizo, Yamasaki, Shinji, Kurazono, Hisao, Faruque, Shah M., ...
We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter...
Faruque, Shah M., Kamruzzaman, M., Meraj, Ismail M., Chowdhury, Nityananda, Nair, G. Balakrish, Sack, R. Bradley, ...
The major virulence factors of toxigenic Vibrio cholerae are cholera toxin (CT), which is encoded by a lysogenic bacteriophage (CTXΦ), and toxin-coregulated pilus (TCP), an essential colonization...
Rahman, Motiur, Mukhopadhyay, Asish K., Nahar, Shamsun, Datta, Simanti, Mashhud Ahmad, Milan, Sarker, Safique, ...
The complex relation between the genotype of Helicobacter pylori and its association with clinical outcome is not well understood. Studies in the West have showed that strains expressing certain...
Environmental Isolates of Aeromonas spp. Harboring the cagA-Like Gene of Helicobacter pylori
Datta, Simanti, Khan, Asis, Nandy, Ranjan K., Rehman, Motiur, Sinha, Sutapa, Chattopadhyay, Santanu, ...
We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a...
Rahman, Motiur, Mukhopadhyay, Asish K., Nahar, Shamsun, Datta, Simanti, Ahmad, Mian Mashhud, Sarker, Safique, ...
Pandey, Mritunjay, Khan, Asis, Das, Suresh C., Sarkar, Bhaswati, Kahali, Soumen, Chakraborty, Subhra, ...
Among Escherichia coli strains isolated from stool specimens from patients with acute diarrhea, 1.4% were found to harbor cdtB by use of enrichment cytolethal distending toxin (CDT) PCR. These...
CTXΦ-independent production of the RS1 satellite phage by Vibrio cholerae
Faruque, Shah M., Kamruzzaman, M., Sack, David A., Mekalanos, John J., Nair, G. Balakrish
The cholera toxin genes of Vibrio cholerae are encoded by the filamentous phage, CTXΦ. Chromosomal CTXΦ prophage DNA is often found flanked by copies of a related genetic element designated RS1,...
Emergence and evolution of Vibrio cholerae O139
Faruque, Shah M., Sack, David A., Sack, R. Bradley, Colwell, Rita R., Takeda, Yoshifumi, Nair, G. Balakrish
The emergence of Vibrio cholerae O139 Bengal during 1992–1993 was associated with large epidemics of cholera in India and Bangladesh and, initially, with a total displacement of the existing V....
Dutta, Shanta, Ghosh, Ayantika, Ghosh, Kaushik, Dutta, Dharitri, Bhattacharya, Sujit K., Nair, G. Balakrish, ...
Shigella dysenteriae Type 1-Specific Bacteriophage from Environmental Waters in Bangladesh
Faruque, Shah M., Chowdhury, Nityananda, Khan, Rasel, Hasan, M. Rubayet, Nahar, Jebun, Islam, M. Johirul, ...
Shigella dysenteriae type 1 is the causative agent of the most severe form of bacillary dysentery, which occurs as epidemics in many developing countries. We isolated a bacteriophage from surface...
Faruque, Shah M., Chowdhury, Nityananda, Kamruzzaman, M., Dziejman, Michelle, Rahman, M. Hasibur, Sack, David A., ...
To understand the evolutionary events and possible selection mechanisms involved in the emergence of pathogenic Vibrio cholerae, we analyzed diverse strains of V. cholerae isolated from environmental...
Chattopadhyay, Santanu, Patra, Rajashree, Ramamurthy, T., Chowdhury, Abhijit, Santra, Amal, Dhali, G. K., ...
We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy...
Antimicrobial Susceptibility of Helicobacter pylori Strains Isolated in Bangladesh
Nahar, Shamsun, Mukhopadhyay, Asish K., Khan, Rasel, Ahmad, Mian Mashhud, Datta, Simanti, Chattopadhyay, Santanu, ...
Antimicrobial susceptibility of 120 Helicobacter pylori isolates to metronidazole, tetracycline, clarithromycin, and amoxicillin was determined, and 77.5, 15, 10, and 6.6% of the isolates,...
Nusrin, Suraia, Khan, G. Yeahia, Bhuiyan, N. A., Ansaruzzaman, M., Hossain, M. A., Safa, Ashrafus, ...
PCR surveillance of the rstR genes of CTX phages in Vibrio cholerae O1 and O139 showed no relationship between the incidence of disease and changes in the rstR but showed variations in their presence...
Faruque, Shah M., Naser, Iftekhar Bin, Islam, M. Johirul, Faruque, A. S. G., Ghosh, A. N., Nair, G. Balakrish, ...
The relationship among (i) the local incidence of cholera, (ii) the prevalence in the aquatic environment of Vibrio cholerae, and (iii) bacterial viruses that attack potentially virulent O1 and O139...
Safa, Ashrafus, Bhuiyan, Nurul Amin, Alam, Munirul, Sack, David A., Nair, G. Balakrish
The genomes of the recently described Matlab variants of Vibrio cholerae O1 that are hybrids between classical and El Tor biotypes were compared with those of El Tor and classical biotypes by the use...
Self-limiting nature of seasonal cholera epidemics: Role of host-mediated amplification of phage
Faruque, Shah M., Islam, M. Johirul, Ahmad, Qazi Shafi, Faruque, A. S. G., Sack, David A., Nair, G. Balakrish, ...
Phage predation of Vibrio cholerae has recently been reported to be a factor that influences seasonal epidemics of cholera in Bangladesh. To understand more about this phenomenon, we studied the...
Ansaruzzaman, M., Lucas, Marcelino, Deen, Jacqueline L., Bhuiyan, N. A., Wang, Xuan-Yi, Safa, Ashrafus, ...
Forty-two episodes of Vibrio parahaemolyticus infections were detected in Beira, Mozambique, from January to May 2004. The majority of the isolates (81%) belonged to the pandemic serovars (O3:K6 and...
Enterotoxigenic Escherichia coli Isolated from Surface Water in Urban and Rural Areas of Bangladesh
Begum, Yasmin A., Talukder, Kaisar A., Nair, G. Balakrish, Qadri, Firdausi, Sack, R. Bradley, Svennerholm, Ann-Mari
Critical Factors Influencing the Occurrence of Vibrio cholerae in the Environment of Bangladesh
Huq, Anwar, Sack, R. Bradley, Nizam, Azhar, Longini, Ira M., Nair, G. Balakrish, Ali, Afsar, ...
The occurrence of outbreaks of cholera in Africa in 1970 and in Latin America in 1991, mainly in coastal communities, and the appearance of the new serotype Vibrio cholerae O139 in India and...
Qadri, Firdausi, Svennerholm, Ann-Mari, Shamsuzzaman, Sohel, Bhuiyan, Taufiqur Rahman, Harris, Jason B., Ghosh, A. N., ...
Vibrio cholerae O139 emerged in 1992 as a major cause of epidemic cholera. However, the incidence of disease due to this new serogroup subsequently decreased for almost a decade. In April 2002, there...
Alam, Munirul, Sadique, Abdus, Bhuiyan, Nurul A., Nair, G. Balakrish, Siddique, A. K., ...
It has long been assumed that prolonged holding of environmental samples at the ambient air temperature prior to bacteriological analysis is detrimental to isolation and detection of Vibrio cholerae,...
Toxigenic Vibrio cholerae in the Aquatic Environment of Mathbaria, Bangladesh
Alam, Munirul, Sultana, Marzia, Nair, G. Balakrish, Sack, R. Bradley, Sack, David A., Siddique, A. K., ...
Toxigenic Vibrio cholerae, rarely isolated from the aquatic environment between cholera epidemics, can be detected in what is now understood to be a dormant stage, i.e., viable but nonculturable when...
Faruque, Shah M., Biswas, Kuntal, Udden, S. M. Nashir, Ahmad, Qazi Shafi, Sack, David A., Nair, G. Balakrish, ...
The factors that enhance the waterborne spread of bacterial epidemics and sustain the epidemic strain in nature are unclear. Although the epidemic diarrheal disease cholera is known to be transmitted...
Alam, Munirul, Hasan, Nur A., Sadique, Abdus, Bhuiyan, N. A., Ahmed, Kabir U., Nusrin, Suraia, ...
Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not...