G. F. Murphy

Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1.

Walsh, L J, Trinchieri, G, Waldorf, H A, Whitaker, D, Murphy, G F

Tumor necrosis factor alpha (TNF-alpha) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate...

Connective tissue mast cells exhibit time-dependent degranulation heterogeneity.

Kaminer, M S, Murphy, G F, Zweiman, B, Lavker, R M

Previous studies have identified two ultrastructurally distinct forms of mast cell (MC) degranulation following activation. Immunoglobulin E (IgE)-mediated reactions are characterized by a very rapid...

Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin.

Yannas, I V, Lee, E, Orgill, D P, Skrabut, E M, Murphy, G F

Regeneration of the dermis does not occur spontaneously in the adult mammal. The epidermis is regenerated spontaneously provided there is a dermal substrate over which it can migrate. Certain highly...

Human/severe combined immunodeficient mouse chimeras. An experimental in vivo model system to study the regulation of human endothelial cell-leukocyte adhesion molecules.

Yan, H C, Juhasz, I, Pilewski, J, Murphy, G F, Herlyn, M, Albelda, S M

The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an...

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Juhasz, I, Lazarus, G S, Murphy, G F, Shih, I M, Herlyn, M

Pemphigus vulgaris is an autoimmune blistering disease that is induced by binding of antibodies to a 130/85-kD protein complex on epidermal keratinocytes. An in vivo experimental model of this...

Degranulation of human mast cells induces an endothelial antigen central to leukocyte adhesion.

Klein, L M, Lavker, R M, Matis, W L, Murphy, G F

To understand better the role of mast cell secretory products in the genesis of inflammation, a system was developed for in vitro degranulation of human mast cells in skin organ cultures. Within 2 hr...

Gamma-interferon inhibits collagen synthesis in vivo in the mouse.

Granstein, R D, Murphy, G F, Margolis, R J, Byrne, M H, Amento, E P

Subcutaneous implantation of osmotic pumps into CAF1 mice resulted in the formation of thick fibrous capsules around the pumps. When pumps were loaded with recombinant murine gamma-interferon...

Isoform I (mdr3) is the major form of P-glycoprotein expressed in mouse brain capillaries. Evidence for cross-reactivity of antibody C219 with an unrelated protein.

Jetté, L, Pouliot, J F, Murphy, G F, Béliveau, R

P-glycoprotein (P-gp) is expressed in various non-cancerous tissues such as the endothelial cells of the blood-brain barrier. We used several monoclonal antibodies (mAbs) and isoform-specific...

Comparative metabolism of d- and l-3,4-dihydroxyphenylaline in normal and pyridoxine-deficient rats

Sourkes, T. L., Wiseman-Distler, Miriam H., Moran, J. F., Murphy, G. F., Saint Cyr, Simonne

Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1.

Walsh, L J, Trinchieri, G, Waldorf, H A, Whitaker, D, Murphy, G F

Tumor necrosis factor alpha (TNF-alpha) is a proinflammatory cytokine that mediates endothelial leukocyte interactions by inducing expression of adhesion molecules. In this report, we demonstrate...

Connective tissue mast cells exhibit time-dependent degranulation heterogeneity.

Kaminer, M S, Murphy, G F, Zweiman, B, Lavker, R M

Previous studies have identified two ultrastructurally distinct forms of mast cell (MC) degranulation following activation. Immunoglobulin E (IgE)-mediated reactions are characterized by a very rapid...

Synthesis and characterization of a model extracellular matrix that induces partial regeneration of adult mammalian skin.

Yannas, I V, Lee, E, Orgill, D P, Skrabut, E M, Murphy, G F

Regeneration of the dermis does not occur spontaneously in the adult mammal. The epidermis is regenerated spontaneously provided there is a dermal substrate over which it can migrate. Certain highly...

Human/severe combined immunodeficient mouse chimeras. An experimental in vivo model system to study the regulation of human endothelial cell-leukocyte adhesion molecules.

Yan, H C, Juhasz, I, Pilewski, J, Murphy, G F, Herlyn, M, Albelda, S M

The ability of circulating white blood cells to enter inflamed tissues is mediated by specific cell adhesion molecules thought to be expressed in a programmed and sequential manner to form an...

Development of pemphigus vulgaris-like lesions in severe combined immunodeficiency disease mice reconstituted with lymphocytes from patients.

Juhasz, I, Lazarus, G S, Murphy, G F, Shih, I M, Herlyn, M

Pemphigus vulgaris is an autoimmune blistering disease that is induced by binding of antibodies to a 130/85-kD protein complex on epidermal keratinocytes. An in vivo experimental model of this...

Degranulation of human mast cells induces an endothelial antigen central to leukocyte adhesion.

Klein, L M, Lavker, R M, Matis, W L, Murphy, G F

To understand better the role of mast cell secretory products in the genesis of inflammation, a system was developed for in vitro degranulation of human mast cells in skin organ cultures. Within 2 hr...

Gamma-interferon inhibits collagen synthesis in vivo in the mouse.

Granstein, R D, Murphy, G F, Margolis, R J, Byrne, M H, Amento, E P

Subcutaneous implantation of osmotic pumps into CAF1 mice resulted in the formation of thick fibrous capsules around the pumps. When pumps were loaded with recombinant murine gamma-interferon...

Isoform I (mdr3) is the major form of P-glycoprotein expressed in mouse brain capillaries. Evidence for cross-reactivity of antibody C219 with an unrelated protein.

Jetté, L, Pouliot, J F, Murphy, G F, Béliveau, R

P-glycoprotein (P-gp) is expressed in various non-cancerous tissues such as the endothelial cells of the blood-brain barrier. We used several monoclonal antibodies (mAbs) and isoform-specific...

Comparative metabolism of d- and l-3,4-dihydroxyphenylaline in normal and pyridoxine-deficient rats

Sourkes, T. L., Wiseman-Distler, Miriam H., Moran, J. F., Murphy, G. F., Saint Cyr, Simonne

Expression and function of endothelial cell alpha v integrin receptors in wound-induced human angiogenesis in human skin/SCID mice chimeras.

Christofidou-Solomidou, M., Bridges, M., Murphy, G. F., Albelda, S. M., DeLisser, H. M.

Accumulating evidence indicates that endothelial cell integrins that bind to the matrix proteins associated with inflammation and wound healing are involved in the process of angiogenesis. The...

Feline atopic dermatitis. A model for Langerhans cell participation in disease pathogenesis.

Roosje, P. J., Whitaker-Menezes, D., Goldschmidt, M. H., Moore, P. F., Willemse, T., Murphy, G. F.

Atopic dermatitis is a disorder characterized by cutaneous exanthemata as a consequence of exaggerated eczematous reactions to topical and systemic allergens. Langerhans cells, expressing CD1a and...

Experimental production and modulation of human cytotoxic dermatitis in human-murine chimeras.

Christofidou-Solomidou, M., Albelda, S. M., Bennett, F. C., Murphy, G. F.

Human dermatitis-involving cytotoxic interaction between effector lymphocytes and epithelial target cells has thus far been documented in vivo only as naturally occurring disease or as an iatrogenic...

Induction of E-selectin-dependent leukocyte recruitment by mast cell degranulation in human skin grafts transplanted on SCID mice.

Christofidou-Solomidou, M., Murphy, G. F., Albelda, S. M.

Previous in vitro data indicate that degranulation of human mast cells triggers the induction of endothelial molecules important in leukocyte adhesion. In vivo experimental systems have not...

Ultrastructure and dynamics of selective mitochondrial injury in carcinoma cells after doxycycline photosensitization in vitro.

Shea, C. R., Whitaker, D., Murphy, G. F., Hasan, T.

Mechanisms and intracellular sites of photosensitized damage were investigated in cultured MGH-U1 cells treated with doxycycline (DOTC). Cells were examined by phase-contrast, fluorescence, and...

Characterization of target injury of murine acute graft-versus-host disease directed to multiple minor histocompatibility antigens elicited by either CD4+ or CD8+ effector cells.

Murphy, G. F., Whitaker, D., Sprent, J., Korngold, R.

The precise identity of effector mononuclear cells capable of eliciting acute graft-versus-host disease (AGVHD) is controversial. In this study, highly purified subsets of donor T cells were used to...

Early cellular events in evolving cutaneous delayed hypersensitivity in humans.

Waldorf, H. A., Walsh, L. J., Schechter, N. M., Murphy, G. F.

The delayed-type hypersensitivity reaction (DHR) in human skin is prototypic for many inflammatory dermatoses. However the cellular events that precede gross lesion formation are unknown. In this...

Hairless micropig skin. A novel model for studies of cutaneous biology.

Lavker, R. M., Dong, G., Zheng, P. S., Murphy, G. F.

Reported here is the structural and immunohistochemical similarities between the Yucatan hairless micropig (HMP) skin and that of humans. Hairless micropig skin surface was composed of complex...

Growth and invasion of human melanomas in human skin grafted to immunodeficient mice.

Juhasz, I., Albelda, S. M., Elder, D. E., Murphy, G. F., Adachi, K., Herlyn, D., ...

An orthotopic model of human melanoma was developed in which malignant cells were injected into human skin grafted to nude and SCID mice. Melanoma cells proliferated and invaded the human skin grafts...

Regulation of extracellular matrix proteins and integrin cell substratum adhesion receptors on epithelium during cutaneous human wound healing in vivo.

Juhasz, I., Murphy, G. F., Yan, H. C., Herlyn, M., Albelda, S. M.

Although changes in extracellular matrix proteins during wound healing have been well documented, little is known about the regulation of corresponding extracellular matrix adhesion receptors...

Phenotypic transformation of macrophages to Langerhans cells in the skin.

Murphy, G. F., Messadi, D., Fonferko, E., Hancock, W. W.

Langerhans cells, important participants in the cutaneous cellular immune response, are markedly diminished in skin of patients undergoing allogeneic bone marrow transplantation during the first 4...

Immunophenotypic characterization of the cutaneous exanthem of SIV-infected rhesus monkeys. Apposition of degenerative Langerhans cells and cytotoxic lymphocytes during the development of acquired immunodeficiency syndrome.

Ringler, D. J., Hancock, W. W., King, N. W., Letvin, N. L., Daniel, M. D., Desrosiers, R. C., ...

A T-cell tropic retrovirus, simian immunodeficiency virus (SIV), has recently been isolated from immunodeficient rhesus monkeys. This virus has remarkable similarities to human immunodeficiency virus...

The genesis of paired cisternae during the mitotic cycle.

Sato, S., Murphy, G. F., Harrist, T. J.

Paired cisternae, which are conspicuous in mitotically active cells, are two or more flattened, membrane-bound saccules stacked in parallel arrays. The origin, development, and fate of paired...

The effect of tropolone on the formation of 3,4-dihydroxyphenylacetic acid and 4-hydroxy-3-methoxyphenylacetic acid in the brain of the mouse

Murphy, G. F., Robinson, D., Sharman, D. F.

1. The development of a very sensitive and specific fluorimetric assay for 3,4-dihydroxyphenylacetic acid has made it possible to measure how inhibitors of the enzyme catechol-O-methyl transferase...