Lichtman, S N, Sherman, P M, Forstner, G G
The effect of starvation on the mucosal secretory immunoglobulin A (sIgA) response to bacterial antigens was studied in bile-free rat self-filling blind loops constructed at the end of a Roux-en-Y...
Colipase and maximally activated pancreatic lipase in normal subjects and patients with steatorrhea.
Gaskin, K J, Durie, P R, Hill, R E, Lee, L M, Forstner, G G
Human pancreatic lipase in duodenal secretions was studied under conditions of maximal activation by porcine colipase and maximal inhibition by sodium taurodeoxycholate. In almost all samples, total...
Qureshi, R, Forstner, G G, Forstner, J F
We have developed a double-antibody radioimmunoassay for the quantitative measurement of human goblet cell mucin (GCM) in order to study intestinal mucus in human and other species. The assay used...
McCool, D J, Marcon, M A, Forstner, J F, Forstner, G G
The T84 colonic adenocarcinoma cell line, which has been used extensively as a model for studies of epithelial chloride secretion, also produces mucin and secretes it in culture. Electron microscopy...
Tissue-specific expression of a rat intestinal mucin-like peptide.
Xu, G, Wang, D, Huan, L J, Cutz, E, Forstner, G G, Forstner, J F
Expression of the gene for a rat intestinal mucin-like peptide (MLP) was studied by Northern-blot analyses of RNA prepared from a panel of rat tissues. Four probes (A-D) were constructed so as to...
Suggestive evidence for two different mucin genes in rat intestine.
Khatri, I A, Forstner, G G, Forstner, J F
In the present report we describe the isolation and sequence of a partial cDNA (M2-798) for a rat intestinal mucin designated M2. A rat intestinal lambda ZAP II cDNA library was screened using a...
McCool, D J, Forstner, J F, Forstner, G G
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete...
Synthesis and secretion of mucin by the human colonic tumour cell line LS180.
McCool, D J, Forstner, J F, Forstner, G G
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media...
Mantle, M, Forstner, G G, Forstner, J F
Previous studies have shown that human small-intestinal mucin consists of high-Mr glycoproteins and a smaller S-S-bonded protein of 118 kDa. The major antigenic determinants of the mucin were...
Immunoassay of serum conjugates of cholic acid in cystic fibrosis.
Davidson, G P, Corey, M, Morad-Hassel, F, Sondheimer, J M, Crozier, D, Forstner, G G
Pre- and post-prandial serum conjugates of cholic acid (SCCA) were measured by radioimmunoassay (RIA) in 83 patients with cystic fibrosis (CF), 14 of whom did not have steatorrhoea, and in 25...
Characterization and localization of the putative 'link' component in rat small-intestinal mucin.
Fahim, R E, Specian, R D, Forstner, G G, Forstner, J F
Rat intestinal mucin is polymerized by a putative 'link' component of Mr 118,000 that can be released from the native mucin by thiol reduction [Fahim, Forstner & Forstner (1983) Biochem. J. 209,...
Fahim, R E, Forstner, G G, Forstner, J F
An investigation was undertaken to discover whether mucin purified from secretions in the lumen of rat small intestine differed in structure or composition from intracellular mucin purified from rat...
Antigenic and structural features of goblet-cell mucin of human small intestine.
Mantle, M, Forstner, G G, Forstner, J F
With the use of a newly developed solid-phase radioimmunoassay method, the major antigenic determinants of human small-intestinal goblet-cell mucin were investigated and related to the overall...
Heterogeneity of rat goblet-cell mucin before and after reduction.
Fahim, R E, Forstner, G G, Forstner, J F
Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. The mucin was applied in low concentrations (1...
Maltase-glucoamylase, a microvillous membrane ectoenzyme, was solubilized from rat intestinal mucosa by digestion with papain and subsequently purified to homogeneity with an overall yield of...
Enhancement of the viscosity of mucin by serum albumin.
List, S J, Findlay, B P, Forstner, G G, Forstner, J F
The interaction of serum albumin with a model epithelial mucin from pig stomach was explored by rotary viscometry. During 30 min of incubation of human serum albumin(20mg/ml) and pig gastric mucin...
Enzyme activity in partly dissociated fragments of rat intestinal maltase/glucoamylase.
Pure rat intestinal maltase/glucoamylase was partially inactivated in 1% sodium dodecyl sulphage by heating at 40--70 degree C for 5 min at pH 7.5, or by lowering the pH to 5.4--6.6 at 24 degree C....
Lipid composition of the isolated rat intestinal microvillus membrane
Forstner, G. G., Tanaka, K., Isselbacher, K. J.
1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was...
Rat intestinal microvillus membranes. Purification and biochemical characterization
Forstner, G. G., Sabesin, S. M., Isselbacher, K. J.
1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar...
Khatri, I A, Forstner, G G, Forstner, J F
The present study reveals that partial proteolytic degradation of rat Muc 2 mucin can occur rapidly even in the presence of a battery of proteinase inhibitors. During the initial steps of...
McCool, D J, Okada, Y, Forstner, J F, Forstner, G G
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to...
Lichtman, S N, Sherman, P M, Forstner, G G
The effect of starvation on the mucosal secretory immunoglobulin A (sIgA) response to bacterial antigens was studied in bile-free rat self-filling blind loops constructed at the end of a Roux-en-Y...
Colipase and maximally activated pancreatic lipase in normal subjects and patients with steatorrhea.
Gaskin, K J, Durie, P R, Hill, R E, Lee, L M, Forstner, G G
Human pancreatic lipase in duodenal secretions was studied under conditions of maximal activation by porcine colipase and maximal inhibition by sodium taurodeoxycholate. In almost all samples, total...
Qureshi, R, Forstner, G G, Forstner, J F
We have developed a double-antibody radioimmunoassay for the quantitative measurement of human goblet cell mucin (GCM) in order to study intestinal mucus in human and other species. The assay used...
McCool, D J, Marcon, M A, Forstner, J F, Forstner, G G
The T84 colonic adenocarcinoma cell line, which has been used extensively as a model for studies of epithelial chloride secretion, also produces mucin and secretes it in culture. Electron microscopy...
Tissue-specific expression of a rat intestinal mucin-like peptide.
Xu, G, Wang, D, Huan, L J, Cutz, E, Forstner, G G, Forstner, J F
Expression of the gene for a rat intestinal mucin-like peptide (MLP) was studied by Northern-blot analyses of RNA prepared from a panel of rat tissues. Four probes (A-D) were constructed so as to...
Suggestive evidence for two different mucin genes in rat intestine.
Khatri, I A, Forstner, G G, Forstner, J F
In the present report we describe the isolation and sequence of a partial cDNA (M2-798) for a rat intestinal mucin designated M2. A rat intestinal lambda ZAP II cDNA library was screened using a...
McCool, D J, Forstner, J F, Forstner, G G
We have shown previously [McCool, Forstner and Forstner (1994) Biochem. J. 302, 111-118] using pulse-chase labelling of mucin with [3H]threonine that LS180 colonic tumour cells synthesize and secrete...
Synthesis and secretion of mucin by the human colonic tumour cell line LS180.
McCool, D J, Forstner, J F, Forstner, G G
Pulse-chase labelling experiments were performed using the mucin-producing colonic carcinoma cell line LS180. Cells were pulsed with [3H]threonine or [3H]glucosamine and chased in complete media...
Mantle, M, Forstner, G G, Forstner, J F
Previous studies have shown that human small-intestinal mucin consists of high-Mr glycoproteins and a smaller S-S-bonded protein of 118 kDa. The major antigenic determinants of the mucin were...
Immunoassay of serum conjugates of cholic acid in cystic fibrosis.
Davidson, G P, Corey, M, Morad-Hassel, F, Sondheimer, J M, Crozier, D, Forstner, G G
Pre- and post-prandial serum conjugates of cholic acid (SCCA) were measured by radioimmunoassay (RIA) in 83 patients with cystic fibrosis (CF), 14 of whom did not have steatorrhoea, and in 25...
Characterization and localization of the putative 'link' component in rat small-intestinal mucin.
Fahim, R E, Specian, R D, Forstner, G G, Forstner, J F
Rat intestinal mucin is polymerized by a putative 'link' component of Mr 118,000 that can be released from the native mucin by thiol reduction [Fahim, Forstner & Forstner (1983) Biochem. J. 209,...
Fahim, R E, Forstner, G G, Forstner, J F
An investigation was undertaken to discover whether mucin purified from secretions in the lumen of rat small intestine differed in structure or composition from intracellular mucin purified from rat...
Antigenic and structural features of goblet-cell mucin of human small intestine.
Mantle, M, Forstner, G G, Forstner, J F
With the use of a newly developed solid-phase radioimmunoassay method, the major antigenic determinants of human small-intestinal goblet-cell mucin were investigated and related to the overall...
Heterogeneity of rat goblet-cell mucin before and after reduction.
Fahim, R E, Forstner, G G, Forstner, J F
Goblet-cell mucin of rat small intestine was purified from mucosal scrapings by using centrifugation, Sepharose 4B and Sepharose 2B chromatography. The mucin was applied in low concentrations (1...
Maltase-glucoamylase, a microvillous membrane ectoenzyme, was solubilized from rat intestinal mucosa by digestion with papain and subsequently purified to homogeneity with an overall yield of...
Enhancement of the viscosity of mucin by serum albumin.
List, S J, Findlay, B P, Forstner, G G, Forstner, J F
The interaction of serum albumin with a model epithelial mucin from pig stomach was explored by rotary viscometry. During 30 min of incubation of human serum albumin(20mg/ml) and pig gastric mucin...
Enzyme activity in partly dissociated fragments of rat intestinal maltase/glucoamylase.
Pure rat intestinal maltase/glucoamylase was partially inactivated in 1% sodium dodecyl sulphage by heating at 40--70 degree C for 5 min at pH 7.5, or by lowering the pH to 5.4--6.6 at 24 degree C....
Lipid composition of the isolated rat intestinal microvillus membrane
Forstner, G. G., Tanaka, K., Isselbacher, K. J.
1. Rat intestinal microvillus plasma membranes were prepared from previously isolated brush borders and the lipid composition was analysed. 2. The molar ratio of cholesterol to phospholipid was...
Rat intestinal microvillus membranes. Purification and biochemical characterization
Forstner, G. G., Sabesin, S. M., Isselbacher, K. J.
1. A technique is described for the removal of subcellular contaminants from intact rat intestinal brush borders, and for the subsequent separation of a microvillus membrane fraction from a fibrillar...
Khatri, I A, Forstner, G G, Forstner, J F
The present study reveals that partial proteolytic degradation of rat Muc 2 mucin can occur rapidly even in the presence of a battery of proteinase inhibitors. During the initial steps of...
McCool, D J, Okada, Y, Forstner, J F, Forstner, G G
Molecular chaperones are presumed to associate with large secretory mucin glycoproteins during their synthesis in the endoplasmic reticulum (ER), but have not been identified to date. We decided to...
Effect of cimetidine and sodium bicarbonate on pancreatic replacement therapy in cystic fibrosis.
Durie, P R, Bell, L, Linton, W, Corey, M L, Forstner, G G
Fifteen patients with cystic fibrosis and pancreatic insufficiency were studied during four randomised seven day treatment periods in which they received only pancreatic supplement (Pancrelipase, 27...
Bile acid secretion in cystic fibrosis: evidence for a defect unrelated to fat malabsorption.
Weizman, Z, Durie, P R, Kopelman, H R, Vesely, S M, Forstner, G G
In order to define basic biliary defects not related to steatorrhoea in cystic fibrosis, we studied 12 control and 18 cystic fibrosis subjects, with a wide range of pancreatic function. Duodenal...
Moore, D J, Forstner, G G, Largman, C, Cleghorn, G J, Wong, S S, Durie, P R
We evaluated serum cationic trypsinogen as a marker of exocrine pancreatic function in children without cystic fibrosis. The ability of this test to determine steatorrhoea of pancreatic origin, and...
Gastritis of the Herniated Stomach in Patients with Esophageal Hiatus Hernia
Seven illustrative cases of gastritis of the herniated stomach in patients with sliding esophageal hiatus hernia are reported. Five had superficial gastritis (three mild, one moderate and one...