G. I. Murray

Publication List Details

Period

1997 - 1999

Number

43

Co-Authors

Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide (1997)

Mckay, J.A., Murray, G.I., Keith, W.N., Mcleod, H.L.

Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial...

Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide

Mckay, J.A., Murray, G.I., Keith, W.N., Mcleod, H.L.

Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial...

Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide.

McKay, J A, Murray, G I, Keith, W N, McLeod, H L

Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial...

Detection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification.

King, G, Chambers, G, Murray, G I

A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on...

In situ hybridisation of albumin mRNA in normal liver and hepatocellular carcinoma with a digoxigenin labelled oligonucleotide probe.

Murray, G I, Paterson, P J, Ewen, S W, Melvin, W T

AIMS: To study the localisation and distribution of albumin mRNA in normal liver and hepatocellular carcinoma by in situ hybridisation with an oligonucleotide probe. METHODS: A 51 base...

Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.

Murray, G I, Duncan, M E, Melvin, W T, Fothergill, J E

AIMS--To investigate the application in immunohistochemistry of gamma-subunit specific anti-peptide monoclonal antibodies to human neurone specific enolase (NSE); and to determine their reactivity...

A new approach to enzyme histochemical analysis of biopsy specimens.

Murray, G I, Ewen, S W

A novel technique combining the freeze drying and embedding in glycol methacrylate at low temperature of tissue permitted the histochemical demonstration of a variety of enzymes, showing maintenance...

Fluorescence-microscopic measurement of intracellular cytochrome P-450 enzyme activity (ethoxyresorufin O-de-ethylation) in unfixed liver section.

Burke, M D, Murray, G I, Lees, G M

A fluorescence-microscopic method has been developed for measurement of the intracellular kinetics of the cytochrome P-450 reaction, ethoxyresorufin O-de-ethylation, in individual hepatocytes in...

Laser capture microscopy

Curran, S, McKay, J A, McLeod, H L, Murray, G I

Human tissues are composed of complex admixtures of different cell types and their biologically meaningful analysis necessitates the procurement of pure samples of the cells of interest. Many...

Application of laser capture microdissection and proteomics in colon cancer

Lawrie, L C, Curran, S, McLeod, H L, Fothergill, J E, Murray, G I

Aims—Laser capture microdissection is a recent development that enables the isolation of specific cell types for subsequent molecular analysis. This study describes a method for obtaining proteome...

Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide.

McKay, J A, Murray, G I, Keith, W N, McLeod, H L

Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial...

Detection of immunoglobulin light chain mRNA by in situ hybridisation using biotinylated tyramine signal amplification.

King, G, Chambers, G, Murray, G I

A highly sensitive method for the light microscopic in situ hybridisation of immunoglobulin light chain mRNA in formalin fixed, paraffin wax embedded sections is reported. This method is based on...

In situ hybridisation of albumin mRNA in normal liver and hepatocellular carcinoma with a digoxigenin labelled oligonucleotide probe.

Murray, G I, Paterson, P J, Ewen, S W, Melvin, W T

AIMS: To study the localisation and distribution of albumin mRNA in normal liver and hepatocellular carcinoma by in situ hybridisation with an oligonucleotide probe. METHODS: A 51 base...

Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.

Murray, G I, Duncan, M E, Melvin, W T, Fothergill, J E

AIMS--To investigate the application in immunohistochemistry of gamma-subunit specific anti-peptide monoclonal antibodies to human neurone specific enolase (NSE); and to determine their reactivity...

A new approach to enzyme histochemical analysis of biopsy specimens.

Murray, G I, Ewen, S W

A novel technique combining the freeze drying and embedding in glycol methacrylate at low temperature of tissue permitted the histochemical demonstration of a variety of enzymes, showing maintenance...

Fluorescence-microscopic measurement of intracellular cytochrome P-450 enzyme activity (ethoxyresorufin O-de-ethylation) in unfixed liver section.

Burke, M D, Murray, G I, Lees, G M

A fluorescence-microscopic method has been developed for measurement of the intracellular kinetics of the cytochrome P-450 reaction, ethoxyresorufin O-de-ethylation, in individual hepatocytes in...

Laser capture microscopy

Curran, S, McKay, J A, McLeod, H L, Murray, G I

Human tissues are composed of complex admixtures of different cell types and their biologically meaningful analysis necessitates the procurement of pure samples of the cells of interest. Many...

Application of laser capture microdissection and proteomics in colon cancer

Lawrie, L C, Curran, S, McLeod, H L, Fothergill, J E, Murray, G I

Aims—Laser capture microdissection is a recent development that enables the isolation of specific cell types for subsequent molecular analysis. This study describes a method for obtaining proteome...

Amplification of fluorescent in situ hybridisation signals in formalin fixed paraffin wax embedded sections of colon tumour using biotinylated tyramide

Mckay, J.A., Murray, G.I., Keith, W.N., Mcleod, H.L.

Fluorescent in situ hybridisation (FISH) is a powerful tool for the evaluation of chromosomal alterations in formalin fixed paraffin wax embedded sections of colorectal cancer. However, initial...

Cytochrome P450 expression in oesophageal cancer.

Murray, G I, Shaw, D, Weaver, R J, McKay, J A, Ewen, S W, Melvin, W T, ...

The cytochrome P450 superfamily of enzymes play a central part in the metabolism of carcinogens and anti-cancer drugs. The expression, cellular localisation, and distribution of different forms of...

Xenobiotic metabolising enzyme expression in colonic neoplasia.

McKay, J A, Murray, G I, Weaver, R J, Ewen, S W, Melvin, W T, Burke, M D

The cytochrome P450, epoxide hydrolase, and glutathione S-transferase enzyme families play an important part in the metabolism of many carcinogens and anti-cancer drugs. The expression of two forms...

Matrix metalloproteinase 13 activity is associated with poor prognosis in colorectal cancer

Leeman, M F, McKay, J A, Murray, G I

Aims: The matrix metalloproteinases (MMPs) are a family of proteolytic enzymes collectively capable of degrading all extracellular matrix components, in particular fibrillar collagen. The importance...

The immunocytochemical localisation and distribution of cytochrome P-450 in normal human hepatic and extrahepatic tissues with a monoclonal antibody to human cytochrome P-450.

Murray, G I, Barnes, T S, Sewell, H F, Ewen, S W, Melvin, W T, Burke, M D

1. The localisation and distribution of cytochrome P-450 in human tissues has been studied by immunocytochemistry using a monoclonal antibody to a major form of human hepatic cytochrome P-450,...

Glutathione localisation in benign and malignant human breast lesions.

Murray, G. I., Burke, M. D., Ewen, S. W.

Reduced glutathione (GSH) has been demonstrated in benign and malignant human breast lesions using a newly developed histofluorescence technique. GSH was present in every lesion and in each case was...

Enhanced expression of cytochrome P450 in stomach cancer.

Murray, G. I., Taylor, M. C., Burke, M. D., Melvin, W. T.

The cytochromes P450 have a central role in the oxidative activation and detoxification of a wide range of xenobiotics, including many carcinogens and several anti-cancer drugs. Thus the cytochrome...

Characterization of dihydropyrimidine dehydrogenase in human colorectal tumours.

McLeod, H. L., Sludden, J., Murray, G. I., Keenan, R. A., Davidson, A. I., Park, K., ...

Dihydropyrimidine dehydrogenase (DPD) is the rate-limiting enzyme for degradation of 5-fluorouracil (5-FU). DPD activity is highly variable in liver and peripheral mononuclear cells (PMNCs) and it...

Heterogeneous nuclear ribonucleoprotein K is over expressed, aberrantly localised and is associated with poor prognosis in colorectal cancer

Carpenter, B, McKay, M, Dundas, S R, Lawrie, L C, Telfer, C, Murray, G I

Heterogeneous ribonucleoprotein K (hnRNP K) is a member of the hnRNP family which has several different cellular roles including transcription, mRNA shuttling, RNA editing and translation. Several...

Characterisation and protein expression profiling of annexins in colorectal cancer

Duncan, R, Carpenter, B, Main, L C, Telfer, C, Murray, G I

The annexins are family of calcium-regulated phospholipid-binding proteins with diverse roles in cell biology. Individual annexins have been implicated in tumour development and progression, and in...

Human papillomavirus 16 infection in adenocarcinoma of the cervix

Chew, G K, Cruickshank, M E, Rooney, P H, Miller, I D, Parkin, D E, Murray, G I

The impact of the success of organised cervical screening programme results in a steady decline of the incidence of squamous cell carcinoma of the cervix but a concomitant increase in the incidence...

Cytochrome P450 CYP3A in human renal cell cancer

Murray, G I, McFadyen, M C E, Mitchell, R T, Cheung, Y-L, Kerr, A C, Melvin, W T

Renal cell cancer is the main malignant tumour of the kidney and has an increasing incidence. This type of tumour has a poor prognosis and shows intrinsic resistance to several anti-cancer drugs. The...

Colorectal cancer genomics: evidence for multiple genotypes which influence survival

Rooney, P H, Boonsong, A, McKay, J A, Marsh, S, Stevenson, D A J, Murray, G I, ...

Colorectal cancer (CRC) is a leading cause of cancer death and the mechanism for variable outcome in this disease is not yet fully understood. It is hypothesized that differences in the genetic...

Cytochrome P450 CYP1B1 over-expression in primary and metastatic ovarian cancer

McFadyen, M C E, Cruickshank, M E, Miller, I D, McLeod, H L, Melvin, W T, Haites, N E, ...

Ovarian cancer is the most frequent cause of death from gynaecological malignancies world wide. Little improvement has been made in the long-term outcome of this disease, with the 5-year survival of...

c-erbB-2 is not a major factor in the development of colorectal cancer

McKay, J A, Loane, J F, Ross, V G, Ameyaw, M-M, Murray, G I, Cassidy, J, ...

We have investigated c-erbB-2 protein expression in a large cohort of well-characterized colorectal tumours, and in a subset of lymph node metastases. We have also evaluated a Val655Ile single...