Scientific Commons: G. J. Belsham

Stabilized baculovirus vector expressing a heterologous gene and GP64 from a single bicistronic transcript (2006)

Pijlman, G.P., Roode, E.C., Fan, X.X., Roberts, L.O., Belsham, G.J., Vlak, J.M., ...

The efficient scale-up of recombinant protein production in insect-cell bioreactors using baculovirus expression vectors is hampered by reductions in yield with increasing viral passage, the...

Cleavage of translation initiation factor 4AI (eIF4AI) but not eIF4AII by foot-and-mouth disease virus 3C protease: identification of the eIF4AI cleavage site (2001)

Lin, W., Ross-Smith, N., Proud, C.G., Belsham, G.J.

The translation initiation factor eIF4A is cleaved within mammalian cells infected by foot-and-mouth disease virus (FMDV). The FMDV 3C protease cleaves eIF4AI (between residues E143 and V144), but...

Strong buffering capacity of insect cells. Implications for the baculovirus expression system. (1995)

Medina, M., Lopez-Rivas, A., Zuidema, D., Belsham, G.J., Domingo, E., Vlak, J.M.

Engineering and immunogenicity of foot-and-mouth disease virus procapsids produced in insect cells. (1995)

Belsham, G.J., Vlak, J.M., Domingo, E., Lombard, M.

Expression of foot-and-mouth disease virus empty capsid particles in insect cells. (1995)

Belsham, G.J., Vlak, J.M., Domingo, E., Lombard, M.

A hybrid baculovirus-bacteriophage T7 transient expression system. (1995)

Broer, R., Belsham, G.J., Oudshoorn, P., Vlak, J.M., Goldbach, R.W.

Unprocessed foot-and-mouth disease virus capsid precursor displays discontinuous epitopes involved in viral neutralization. (1994)

Cairo, J., Medina, M., Zuidema, D., Abrams, C., Belsham, G.J., ...

Myristoylation of foot-and-mouth disease virus capsid protein precursors is independent of other viral proteins and occurs in both mammalian and insect cells. (1991)

Belsham, G.J., Abrams, C.C., King, A.M.Q., Roosien, J., Vlak, J.M.

Synthesis of foot-and-mouth disease virus capsid proteins in insect cells using baculovirus expression vectors. (1990)

Roosien, J., Belsham, G.J., King, A.M.Q., Ryan, M.D., Vlak, J.M.

Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. (1990)

Zuidema, D., Schouten, A., Usmany, M., Maule, A.J., Belsham, G.J., Roosien, J., ...

Expression of cauliflower mosaic virus gene I using a baculovirus vector based upon the p10 gene and a novel selection method. (1990)

Vlak, J.M., Schouten, A., Usmany, M., Belsham, G.J., Klinge-Roode, E.C., Maule, A.J., ...

Novel screening of baculovirus recombinants facilitated by constitutive expression of ß-galactosidase. (1990)

Zuidema, D., Roosien, J., Belsham, G.J., Maule, A.J., Vlak, J.M.

Expression of foot-and-mouth disease virus proteins in insect cells. (1990)

Roosien, J., Belsham, G.J., Ryan, M.D., King, A.M.Q., Vlak, J.M.

Activation of the translational suppressor 4E-BP1 following infection with encephalomyocarditis virus and poliovirus.

Gingras, A C, Svitkin, Y, Belsham, G J, Pause, A, Sonenberg, N

Infection of cells with picornaviruses, such as poliovirus and encephalomyocarditis virus (EMCV), causes a shutoff of host protein synthesis. The molecular mechanism of the shutoff has been partly...

Viral RNA modulates the acid sensitivity of foot-and-mouth disease virus capsids.

Curry, S, Abrams, C C, Fry, E, Crowther, J C, Belsham, G J, Stuart, D I, ...

Foot-and-mouth disease virus (FMDV) manifests an extreme sensitivity to acid, which is thought to be important for entry of the RNA genome into the cell. We have compared the low-pH-induced...

The La autoantigen contains a dimerization domain that is essential for enhancing translation.

Craig, A W, Svitkin, Y V, Lee, H S, Belsham, G J, Sonenberg, N

The La autoantigen is an RNA-binding protein that is involved in initiation and termination of RNA polymerase III transcription. It also binds several viral RNAs, including those of poliovirus and...

Unprocessed foot-and-mouth disease virus capsid precursor displays discontinuous epitopes involved in viral neutralization.

Sáiz, J C, Cairó, J, Medina, M, Zuidema, D, Abrams, C, Belsham, G J, ...

A foot-and-mouth disease virus (FMDV) cDNA cassette containing sequences encoding the capsid precursor P1, peptide 2A and a truncated 2B (abbreviated P1-2A) of type C FMDV, has been modified to...

trans complementation by RNA of defective foot-and-mouth disease virus internal ribosome entry site elements.

Drew, J, Belsham, G J

A region of about 435 bases from the 5' noncoding region of foot-and-mouth disease virus RNA directs internal initiation of protein synthesis. This region, termed the internal ribosome entry site...

A single nucleotide substitution in the internal ribosome entry site of foot-and-mouth disease virus leads to enhanced cap-independent translation in vivo.

Martínez-Salas, E, Sáiz, J C, Dávila, M, Belsham, G J, Domingo, E

Mutants of foot-and-mouth disease virus (FMDV) with altered biological properties can be selected during the course of persistent infection of BHK-21 cells with FMDV C-S8c1 (J. C. de la Torre, E....

trans complementation of cap-independent translation directed by poliovirus 5' noncoding region deletion mutants: evidence for RNA-RNA interactions.

Stone, D M, Almond, J W, Brangwyn, J K, Belsham, G J

Poliovirus (PV) RNA is translated by a cap-independent mechanism involving the internal entry of ribosomes onto the 5' noncoding region (NCR). Using the vaccinia virus-T7 RNA polymerase transient...

RNA-protein interactions in regulation of picornavirus RNA translation.

Belsham, G J, Sonenberg, N

The translation of picornavirus RNA occurs by a cap-independent mechanism directed by a region of about 450 nucleotides from the 5' untranslated region, termed an internal ribosome entry site (IRES)....

Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis.

Percy, N, Belsham, G J, Brangwyn, J K, Sullivan, M, Stone, D M, Almond, J W

A series of genetic deletions based partly on two RNA secondary structure models (M. A. Skinner, V. R. Racaniello, G. Dunn, J. Cooper, P. D. Minor, and J. W. Almond, J. Mol. Biol. 207:379-392, 1989;...

Chimeric polioviruses that include sequences derived from two independent antigenic sites of foot-and-mouth disease virus (FMDV) induce neutralizing antibodies against FMDV in guinea pigs.

Kitson, J D, Burke, K L, Pullen, L A, Belsham, G J, Almond, J W

Five poliovirus recombinants containing sequences corresponding to foot-and-mouth disease virus (FMDV) antigenic sites were constructed. Viable virus was recovered from four of these plasmids, in...

A region of the 5' noncoding region of foot-and-mouth disease virus RNA directs efficient internal initiation of protein synthesis within cells: involvement with the role of L protease in translational control.

Belsham, G J, Brangwyn, J K

Plasmids encoding bicistronic mRNAs have been constructed and used to identify a region from the 5' noncoding region of foot-and-mouth disease virus (FMDV) which directs efficient internal initiation...

Biochemical properties of the 145,000-dalton super-T antigen from simian virus 40-transformed BALB/c 3T3 clone 20 cells.

Chaudry, F, Belsham, G J, Smith, A E

SV3T3 C120 cells contain a 145,000-dalton form of simian virus 40 (SV40) super-T antigen but little if any normal-sized large-T. The subcellular location of super-T, its DNA binding properties, and...

Target-specific arrest of mRNA translation by antisense 2'-O-alkyloligoribonucleotides.

Johansson, H E, Belsham, G J, Sproat, B S, Hentze, M W

We describe a novel experimental approach to investigate mRNA translation. Antisense 2'-O-allyl oligoribonucleotides (oligos) efficiently arrest translation of targeted mRNAs in rabbit reticulocyte...

An antibody to a synthetic peptide recognizes polyomavirus middle-T antigen and reveals multiple in vitro tyrosine phosphorylation sites.

Harvey, R, Oostra, B A, Belsham, G J, Gillett, P, Smith, A E

Antibodies were raised against three synthetic peptides corresponding to sequences surrounding tyrosine 315, a putative in vitro phosphorylation site in polyomavirus middle-T antigen. Only one of the...

Translation of encephalomyocarditis virus RNA: parameters influencing the selection of the internal initiation site.

Kaminski, A, Belsham, G J, Jackson, R J

The initiation of encephalomyocarditis virus RNA translation is by internal ribosome entry almost exclusively at the 11th AUG codon from the 5'-end, which is the central of the three AUG codons in...

Dual initiation sites of protein synthesis on foot-and-mouth disease virus RNA are selected following internal entry and scanning of ribosomes in vivo.

Belsham, G J

The initiation of protein synthesis on foot-and-mouth disease virus RNA occurs at two sites separated by 84 nucleotides. Immediately upstream from the first of these sites is the internal ribosome...

Reversibility of the insulin-stimulated phosphorylation of ATP citrate lyase and a cytoplasmic protein of subunit Mr 22000 in adipose tissue.

Belsham, G J, Brownsey, R W, Denton, R M

Exposure of rat epididymal adipose tissue to insulin leads to a 2-fold increase in incorporation of [32P]phosphate into ATP citrate lyase and a cytoplasmic protein of Mr 22000. A smaller increase and...

Use of a novel rapid preparation of fat-cell plasma membranes employing Percoll to investigate the effects of insulin and adrenaline on membrane protein phosphorylation within intact fat-cells.

Belsham, G J, Denton, R M, Tanner, M J

1. A rapid method was developed for the preparation of plasma membranes from either isolated rat fat-cells or intact epididymal fat-pads with the use of density-gradient centrifugation in the...

Recognition of picornavirus internal ribosome entry sites within cells; influence of cellular and viral proteins.

Roberts, L O, Seamons, R A, Belsham, G J

The ability of different picornavirus internal ribosome entry site (IRES) elements to direct initiation of protein synthesis has been assayed in different cell lines in the presence and absence of...

A selection system for functional internal ribosome entry site (IRES) elements: analysis of the requirement for a conserved GNRA tetraloop in the encephalomyocarditis virus IRES.

Robertson, M E, Seamons, R A, Belsham, G J

Picornavirus internal ribosome entry site (IRES) elements direct cap-independent internal initiation of protein synthesis within mammalian cells. These RNA elements (about 450 nt) contain extensive...

A novel protein-RNA binding assay: functional interactions of the foot-and-mouth disease virus internal ribosome entry site with cellular proteins.

Stassinopoulos, I A, Belsham, G J

Translation initiation on foot-and-mouth disease virus (FMDV) RNA occurs by a cap-independent mechanism directed by a highly structured element (approximately 435 nt) termed an internal ribosome...

The requirement for eukaryotic initiation factor 4A (elF4A) in translation is in direct proportion to the degree of mRNA 5' secondary structure.

Svitkin, Y V, Pause, A, Haghighat, A, Pyronnet, S, Witherell, G, Belsham, G J, ...

Eukaryotic initiation factor (elF) 4A functions as a subunit of the initiation factor complex elF4F, which mediates the binding of mRNA to the ribosome. elF4A possesses ATPase and RNA helicase...