G. J. G. Ruijter

Regulation of pentose catabolic pathway genes of Aspergillus niger (2007)

Wosten, H.A.B., Levisson, M., VanKuyk, P.A., Ruijter, G.J.G., ...

The aim of this study was to obtain a better understanding of the pentose catabolism in Aspergillus niger and the regulatory systems that affect it. To this end, we have cloned and characterised the...

Glucose uptake and growth of glucose-limited chemostat cultures of Aspergillus niger and a disruptant lacking MstA, a high-affinity glucose transporter (2007)

Jorgensen, T.R., VanKuyk, P.A., Poulsen, B.R., Ruijter, G.J.G., Visser, J., Iversen, J.J.L.

Selection and characterisation of a xylitol-derepressed Aspergillus niger mutant that is apparently impaired in xylitol transport (2006)

Ruijter, G.J.G., Visser, J.

Aspergillus niger is known for its biotechnological applications, such as the use of xylanase enzyme for the degradation of hemicellulose. Depending on culture conditions, several polyols may also be...

Metabolic control analysis of Aspergillus niger L-arabinose catabolism (2005)

Prathumpai, W., Visser, J., Ruijter, G.J.G.

A mathematical model of the L-arabinose/D-xylose catabolic pathway of Aspergillus niger was constructed based on the kinetic properties of the enzymes. For this purpose L-arabinose reductase,...

Increased NADPH concentration obtained by metabolic engineering of the pentose phosphate pathway in Aspergillus niger (2005)

Poulsen, B.R., Nohr, J., Douthwaite, S., Hansen, L.V., Iversen, J.J.L., Visser, J., ...

Many biosynthetic reactions and bioconversions are limited by low availability of NADPH. With the purpose of increasing the NADPH concentration and/or the flux through the pentose phosphate pathway...

Polyol accumulation by Aspergillus oryzae at low water activity in solid-state fermentation (2004)

Ruijter, G.J.G., Visser, J., Rinzema, A.

Polyol accumulation and metabolism were examined in Aspergillus oryzae cultured on whole wheat grains or on wheat dough as a model for solid-state culture. In solid-state fermentation (SSF), water...

Aspergillus niger mstA encodes a high affinity sugar/H+ symporter which is regulated in persponse to extracellular pH (2004)

Diderich, J.A., MacCabe, A.P., Hererro, O., Ruijter, G.J.G., Visser, J.

A sugar-transporter-encoding gene, mstA, which is a member of the major facilitator superfamily, has been cloned from a genomic DNA library of the filamentous fungus Aspergillus niger. To enable the...

Isolation of a fluffy mutant of Aspergillus niger from chemostat culture and its potential use as a morphologically stable host for protein production (2004)

Poulsen, B.R., Ruijter, G.J.G., Schuleit, T., Visser, J., Iversen, J.J.L.

Chemostat cultivation of Aspergillus niger and other filamentous fungi is often hindered by the spontaneous appearance of morphologic mutants. Using the Variomixing bioreactor and applying different...

Fast response filter module with plug flow of filtrate for on-line sampling from submerged cultures of filamentous fungi (2004)

Poulsen, B.R., Ruijter, G.J.G., Panneman, H., Iversen, J.J.L., Visser, J.

Automatic and accurate sampling is both convenient and sometimes necessary to obtain detailed information about cell cultures. We developed an autoclavable sampling system in which culture broth was...

Characterization of nerolidol biotransformation based on indirect on-line estimation of biomass concentration and physiological state in batch cultures of Aspergillus niger (2004)

Ruijter, G.J.G., Visser, J., Iversen, J.J.L.

Biotransformation of the sesquiterpenoid trans-nerolidol by Aspergillus niger has previously been investigated as a method for the formation of 12-hydroxy- trans-nerolidol, a precursor in the...

Glycerol dehydrogenase, encoded by gldB is essential to osmotolerance in Aspergillus nidulans (2003)

Flitter, S.J., Chaveroche, M.K., Fontaine, T., Fillinger, S., ...

Mannitol is required for stress tolerance in Aspergillus niger conidiospores (2003)

Ruijter, G.J.G., Bax, M., Patel, H., Flitter, S.J., ...

D-Mannitol is the predominant carbon compound in conidiospores of the filamentous fungus Aspergillus niger and makes up 10 to 15␘f the dry weight. A number of physiological functions have been...

Onset of carbon catabolite repression in Aspergillus nidulans (2003)

Flipphi, M., Ruijter, G.J.G., Visser, J., Arst Jr., H.N., Felenbok, B.

Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism (2003)

Ruijter, G.J.G., Visser, J.

Production of Organic Acids by Fungi (2002)

Ruijter, G.J.G, Kubicek, C.P., Visser, J.

Intracellular pH homeostasis in the filamentous fungus Aspergillys niger (2002)

Hesse, S.J.A., Ruijter, G.J.G., Dijkema, C., Visser, J.

Intracellular pH homeostasis in the filamentous fungus Aspergillus niger was measured in real time by 31P NMR during perfusion in the NMR tube of fungal biomass immobilized in Ca2 -alginate beads....

Cyclic AMP-dependent protein kinase is involved in morphogenesis of Aspergillus niger (2002)

Staudohar, M., Bencina, M., Panneman, H., Legisa, M., Visser, J., ...

The Aspergillus niger D-xylulose kinase gene is co-expressed with genes encoding arabinan degrading enzymes, and is essential for growth on D-xylose and L-arabinose (2001)

Ruijter, G.J.G., Visser, J.

The Aspergillus nigerd-xylulose kinase encoding gene has been cloned by complementation of a strain deficient in d-xylulose kinase activity. Expression of xkiA was observed in the presence of...

Measurement of Intracellular (compartmental) pH by 31P NMR in Aspergillus niger (2000)

Hesse, S.J.A., Ruijter, G.J.G., Dijkema, C., Visser, J.

Characterisation of Aspergillus niger phosphoglucose isomerase. Use for quantitative determination of erythrose 4-phosphate (1999)

Ruijter, G.J.G., Visser, J.

Phosphoglucose isomerase (PGI) was purified from Aspergillus niger and the in vitro kinetic properties of the enzyme were related to its functioning in vivo. A new assay method was developed to study...

Oxalic acid production by Aspergillus niger: an oxalate non-producing mutant produces citric acid at pH 5 and in the presence of manganese (1999)

Ruijter, G.J.G., Visser, J.

Cloning and biochemical characterisation of Aspergillus niger hexokinase: the enzyme is strongly inhibited by physiological concentrations of the trehalose-6-phosphate. (1998)

Panneman, H., Ruijter, G.J.G., Visser, J.

Metabolic engineering of the glycolytic pathway in Aspergillus niger. (1998)

Ruijter, G.J.G., Panneman, H., Visser, J.

Strain improvement. (1998)

Ruijter, G.J.G., Visser, J.

Characterization and overexpression of the Aspergillus niger gene encoding the cAMP-dependent protein kinase catalytic subunit. (1997)

Bencina, M., Panneman, H., Ruijter, G.J.G., Legisa, M., Visser, J.

Overexpression of phosphofructokinase and pyruvate kinase in citric acid producing Aspergillus niger. (1997)

Ruijter, G.J.G., Panneman, H., Visser, J.

Carbon repression in Aspergilli. (1997)

Ruijter, G.J.G., Visser, J.

Many microorganisms prefer easily metabolizable carbon sources over alternative, less readily metabolized carbon sources. One of the mechanisms to achieve this is repression of the synthesis of...

Isolation of Aspergillus niger creA mutants. Effects on expression of arabinases and L-arabinose catabolic enzymes. (1997)

Ruijter, G.J.G., Vanhanen, S.A., Gielkens, M.M.C., Visser, J.

Cloning and biochemical characterisation of an Aspergillus niger glucokinase. Evidence for the presence of a separate glucokinase and hexokinase enzymes. (1996)

Panneman, H., Ruijter, G.J.G., Driever, E.T.M., Visser, J.

Characterisation of the Aspergillus nidulans frA1 mutant: hexose phosphorylation and apparent lack of involvement of hexokinase in glucose repression. (1996)

Ruijter, G.J.G., Panneman, H., Bennett, J.M., Visser, J.

Hexose phosphorylation was studied in Aspergillus nidulans wild-type and in a fructose non-utilising mutant (frA). The data indicate the presence of at least one hexokinase and one glucokinase in...

Determination of intermediary metabolites in Aspergillus niger. (1996)

Ruijter, G.J.G., Visser, J.

Modelling of carbohydrate metabolism in citric acid producing Aspergillus niger. (1996)

Ruijter, G.J.G., Snoep, J.L., Visser, J.

Physiological consequences of an extreme Aspergillus nidulans creA mutation on D-glucose utilization. (1995)

Ruijter, G.J.G., Visser, J.

An automated monitoring system using on-line ultrafiltration and column liquid chromatography for Aspergillus niger fermentations. (1994)

Ruijter, G.J.G., Lingeman, H., Brinkman, U.A.Th., Visser, J.

Enzymes II of the phosphotransferase system: transport and regulation. (1994)

Postma, P.W., Vlag, J. Van Der, Waard, J.H. De, Dam, K. Van, Ruijter, G.J.G.