Scientific Commons: G. J. Tyrrell

Alteration of the carbohydrate binding specificity of verotoxins from Gal alpha 1-4Gal to GalNAc beta 1-3Gal alpha 1-4Gal and vice versa by site-directed mutagenesis of the binding subunit.

Tyrrell, G J, Ramotar, K, Toye, B, Boyd, B, Lingwood, C A, Brunton, J L

Verotoxin 1 (VT-1) and Shiga-like toxin II (SLT-II) bind to the glycosphingolipid (GSL), globotriaosylceramide (Gb3), whereas pig edema disease toxin (VTE) binds to globotetraosylceramide (Gb4) and...

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

MacKinnon, M G, Drebot, M A, Tyrrell, G J

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion...

Species identification of enterococci via intergenic ribosomal PCR.

Tyrrell, G J, Bethune, R N, Willey, B, Low, D E

Accurate species identification of enterococci has become important with the wide prevalence of acquired vancomycin resistance and the presence of less epidemiologically important, inherently...

Lectinlike properties of pertussis toxin.

Tyrrell, G J, Peppler, M S, Bonnah, R A, Clark, C G, Chong, P, Armstrong, G D

We have examined the lectinlike properties of pertussis toxin by binding-inhibition assays and affinity chromatography of goose erythrocyte membranes. Although pertussis toxin and wheat germ...