Herzmann, C., Cuthbertson, Z., Fosdick, L., Fisher, M., Nelson, M., Perry, N., ...
Objectives Subcutaneous administration of interleukin-2 (IL-2) has been shown to increase CD4 counts in HIV-infected patients. It remains unclear whether this effect is associated with a clinical...
WILLCOX, N., SCHLUEPT, M., SOMMER, N., CAMPANA, D., JANOSSY, G., BROWN, A. N., ...
The thymus has been studied in myasthenia gravis patients to assess the effects of previous immunosuppresslon on total yields of cell suspension, immunohistology and culture responses. The reduction...
Koene, R. A. P., Capel, P. J. A., Dewitte, T. J. M., Leeuwenberg, J., Willems, R., Trejdosiewicz, L., ...
Goodall, Alison H., Kemble, G., O'Brien, D.P., Rawlings, E., Rotblat, F., Russell, G.C., ...
A murine hybridoma clone is described that grows continuously in culture and produces a monoclonal antibody we have called Royal Free Monoclonal Antibody to factor IX No. 1 (RFF-lX/1 ). This has high...
Polyclonal activation of murine and human T and B lymphocytes /--George Janossy. (1974)
Includes bibliographical references.
Identification of malignant plasma cell precursors in the bone marrow of multiple myeloma.
Caligaris-Cappio, F, Bergui, L, Tesio, L, Pizzolo, G, Malavasi, F, Chilosi, M, ...
Precursors of plasma cells were studied in the bone marrow of 28 patients with multiple myeloma, plasma cell leukemia, and benign monoclonal gammopathy. Pre-B and B cell populations were analyzed...
Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X.
Thomas, J A, Janossy, G, Chilosi, M, Pritchard, J, Pincott, J R
The immunological phenotype of the cells involved in skin and lymph node lesions from two cases of histiocytosis X (H-X) were analysed by immunofluorescence techniques using combinations of...
Enzyme histochemical analysis on cryostat sections of human bone marrow.
Chilosi, M, Pizzolo, G, Janossy, G, Bofill, M, Fiore-Donati, L
A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether...
Selby, W S, Poulter, L W, Hobbs, S, Jewell, D P, Janossy, G
HLA-DR-positive histiocytes in the lamina propria of the human intestine have been characterised using combined histochemical and immunohistological techniques. In the small intestine, 80-90% of the...
Raftery, M J, Poulter, L W, Janossy, G, Sweny, P, Fernando, O N, Moorhead, J F
Biopsies of normal kidneys taken at time of transplantation were studied using a variety of immunofluorescent and cytochemical techniques. A heterogeneous population of HLA-DR+ cells was found,...
Trejdosiewicz, L K, Malizia, G, Oakes, J, Losowsky, M S, Janossy, G
Expression of the gp100 common acute lymphoblastic leukaemia antigen (CALLA) was studied in the mucosa of the gut by means of indirect immunofluorescence on cryostat tissue sections with a panel of...
Enzyme and membrane markers in leukaemia: recent developments.
Terminal deoxynucleotidyl transferase (TdT) assay has proved a valuable test for distinguishing lymphoblastic from myeloblastic leukaemias, particularly in adults whose blast cells are often negative...
Meningeal leukaemia in lymphoid blast crisis of chronic myeloid leukaemia.
Woodruff, R K, Malpas, J S, Wrigley, P F, Lister, T A, Paxton, A M, Janossy, G
Open access clinic providing HIV-I antibody results on day of testing: the first twelve months.
Squire, S B, Elford, J, Bor, R, Tilsed, G, Salt, H, Bagdades, E K, ...
OBJECTIVES--To determine the sociodemographic profile, risk category, and prevalence of HIV-I infection among people attending a clinic providing counselling, medical advice, and results of HIV-I...
Lee, C A, Phillips, A N, Elford, J, Janossy, G, Griffiths, P, Kernoff, P
OBJECTIVE--To describe the progression of HIV disease in a haemophilic cohort and to show the influence of treatment. DESIGN--11 year longitudinal clinical and laboratory study. SETTING--A...
Identification of malignant plasma cell precursors in the bone marrow of multiple myeloma.
Caligaris-Cappio, F, Bergui, L, Tesio, L, Pizzolo, G, Malavasi, F, Chilosi, M, ...
Precursors of plasma cells were studied in the bone marrow of 28 patients with multiple myeloma, plasma cell leukemia, and benign monoclonal gammopathy. Pre-B and B cell populations were analyzed...
Combined immunological and histochemical analysis of skin and lymph node lesions in histiocytosis X.
Thomas, J A, Janossy, G, Chilosi, M, Pritchard, J, Pincott, J R
The immunological phenotype of the cells involved in skin and lymph node lesions from two cases of histiocytosis X (H-X) were analysed by immunofluorescence techniques using combinations of...
Enzyme histochemical analysis on cryostat sections of human bone marrow.
Chilosi, M, Pizzolo, G, Janossy, G, Bofill, M, Fiore-Donati, L
A recently developed procedure, that has been shown to be suitable for detailed immunohistological analysis, has been used to prepare cryostat sections of bone marrow to investigate whether...
Selby, W S, Poulter, L W, Hobbs, S, Jewell, D P, Janossy, G
HLA-DR-positive histiocytes in the lamina propria of the human intestine have been characterised using combined histochemical and immunohistological techniques. In the small intestine, 80-90% of the...
Raftery, M J, Poulter, L W, Janossy, G, Sweny, P, Fernando, O N, Moorhead, J F
Biopsies of normal kidneys taken at time of transplantation were studied using a variety of immunofluorescent and cytochemical techniques. A heterogeneous population of HLA-DR+ cells was found,...
Trejdosiewicz, L K, Malizia, G, Oakes, J, Losowsky, M S, Janossy, G
Expression of the gp100 common acute lymphoblastic leukaemia antigen (CALLA) was studied in the mucosa of the gut by means of indirect immunofluorescence on cryostat tissue sections with a panel of...
Enzyme and membrane markers in leukaemia: recent developments.
Terminal deoxynucleotidyl transferase (TdT) assay has proved a valuable test for distinguishing lymphoblastic from myeloblastic leukaemias, particularly in adults whose blast cells are often negative...
Meningeal leukaemia in lymphoid blast crisis of chronic myeloid leukaemia.
Woodruff, R K, Malpas, J S, Wrigley, P F, Lister, T A, Paxton, A M, Janossy, G
Open access clinic providing HIV-I antibody results on day of testing: the first twelve months.
Squire, S B, Elford, J, Bor, R, Tilsed, G, Salt, H, Bagdades, E K, ...
OBJECTIVES--To determine the sociodemographic profile, risk category, and prevalence of HIV-I infection among people attending a clinic providing counselling, medical advice, and results of HIV-I...
Lee, C A, Phillips, A N, Elford, J, Janossy, G, Griffiths, P, Kernoff, P
OBJECTIVE--To describe the progression of HIV disease in a haemophilic cohort and to show the influence of treatment. DESIGN--11 year longitudinal clinical and laboratory study. SETTING--A...
Akbar, A N, Timms, A, Janossy, G
After T-cell activation, a switch of leucocyte common antigen (LCA) expression occurs which is detected by the disappearance of CD45R and the appearance of UCHL1 positivity. Upon activation of CD45R+...
The tissue distribution of T lymphocytes expressing different CD45 polypeptides.
Janossy, G, Bofill, M, Rowe, D, Muir, J, Beverley, P C
The distribution of T lymphocytes expressing the different polypeptides of the leucocyte common antigen (LCA) family detected by CD45R and UCHL1 antibodies has been studied in normal lymphoid...
Huby, R D, Janossy, G, Lampert, I A
Cyclosporin A (CsA) is known to cause atrophy of the thymic medulla in normal mice. We show that medullary regeneration in lethally irradiated animals reconstituted with syngeneic marrow is also...
Janossy, G., Shohat, Maya, Greaves, M. F., Dourmashkin, R. R.
Thymocytes from cortisone-treated mice (`T' cells), `B' spleen cells (B lymphocytes from thymectomized, irradiated, marrow reconstituted mice) and normal spleen (T + B) cells were examined by...
Patterns of B-lymphocyte gene expression elicited by lipopolysaccharide mitogen.
Janossy, G, Snajdr, J, Simak-Ellis, M
When large proportions of B lymphocytes from the murine spleen are stimulated in vitro by bacterial lipopolysaccharide (LPS) B lymphoblasts with small amounts of intracellular immunoglobulin (Ig) and...
Enumeration of polyclonal mitogen-responsive cells in different lymphoid tissues of the mouse.
Doenhoff, M J, Janossy, G, Kerbel, R S
The relative number of cells capable of responding to Con A, PHA and LPS in the spleen, blood, lymph node and Peyer's patches of CBA mice has been quantified by means of a cytological analysis...
Gombert, W, Borthwick, N J, Wallace, D L, Hyde, H, Bofill, M, Pilling, D, ...
The apoptosis of human cytokine-deprived activated T cells can be prevented by a soluble mediator secreted by fibroblasts, epithelial and endothelial cells, and this rescue occurs with fibroblasts...
Borthwick, N J, Bofill, M, Hassan, I, Panayiotidis, P, Janossy, G, Salmon, M, ...
The expanded CD8+ T-lymphocyte population arising in response to viral infection controls the virus but could also prove damaging to the host unless safely removed at the end of the immune response....
Duke, O, Panayi, G S, Janossy, G, Poulter, L W, Tidman, N
In an attempt to define the immunoregulatory mechanisms operating in rheumatoid arthritis we have enumerated T cell subsets in the peripheral blood and synovial fluid of patients with this disease....
Bofill, M., Gombert, W., Borthwick, N. J., Akbar, A. N., McLaughlin, J. E., Lee, C. A., ...
Infection with human immunodeficiency virus 1 causes profound changes in the lymph nodes of infected patients. In particular, large numbers of CD8+CD45RO+ T cells infiltrate both the paracortex and...
Selby, W S, Janossy, G, Jewell, D P
Intraepithelial lymphocytes (IEL) of the normal human stomach, small intestine, and large intestine have been characterised in tissue sections by a double marker immunofluorescent technique. A panel...
Selby, W S, Janossy, G, Bofill, M, Jewell, D P
Lymphocyte subpopulations in the intestinal mucosa of patients with ulcerative colitis or Crohn's disease have been studied using a double marker immunofluorescence technique. Analysis of tissue...
Microenvironments in the normal thymus and the thymus in myasthenia gravis.
Bofill, M., Janossy, G., Willcox, N., Chilosi, M., Trejdosiewicz, L. K., Newsom-Davis, J.
The disposition of epithelial cells and extracellular matrix, in the thymus of 8 cases of myasthenia gravis (MG) and in controls (over a wide age range) was studied. In the controls, the subcapsular...
Tenner-Racz, Klara, Racz, P., Bofill, Margarita, Schulz-Meyer, Anke, Dietrich, M., Kern, P., ...
The presence of core antigens of retrovirus HTLV-III/LAV, referred to as “AIDS-related virus” (AV), has been sought in lymph node samples of patients with persistent generalized lymphadenopathy...
Akbar, A N, Amlot, P L, Timms, A, Lombardi, G, Lechler, R, Janossy, G
In normal individuals, 80 +/- 5% of circulating CD8+ T cells express CD45RA, and 20 +/- 7% of these cells express CD45R0 antigens. After activation, CD8+ cells expressing CD45RA decrease to 56-67%...
Noble, B, Ren, K, Taverne, J, Dipirro, J, Van Liew, J, Dijkstra, C, ...
Immunohistochemical methods were used to investigate the role of macrophages in the progression of proliferative immune complex glomerulonephritis. The mononuclear cell component of glomerular...
Selby, W S, Janossy, G, Bofill, M, Jewell, D P
Lymphocyte subpopulations in human small intestinal mucosa have been studied using an immunofluorescence technique on tissue sections. In the normal intestine, the majority of intraepithelial...
Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease.
Selby, W S, Janossy, G, Mason, D Y, Jewell, D P
The expression of HLA-DR and HLA-A,B,C antigens by human colonic epithelium has been examined in tissue sections of patients with inflammatory bowel disease using an immunohistological technique....
Tax, W J, Tidman, N, Janossy, G, Trejdosiewicz, L, Willems, R, Leeuwenberg, J, ...
WT 1, an IgG2a subclass monoclonal antibody, recognizes a human T lineage specific antigen (mol. wt 40,000). This antigen is strongly expressed on thymic T blasts, and on peripheral T cells activated...
Pizzolo, G, Semenzato, G, Chilosi, M, Morittu, L, Ambrosetti, A, Warner, N, ...
When studied with double staining techniques HNK-1+ cells include subsets not expressing T cell antigens (A), expressing T8 antigens (B) and expressing T4 antigens (C). Cells with phenotype A are...
Mishra, B B, Poulter, L W, Janossy, G, James, D G
Immunohistological observations of lymphoid and non-lymphoid cell subsets in biopsies of sarcoid skin granulomas have been compared with positive Kveim tests and the sites of PPD injection in sarcoid...
T cell regeneration after allogenic bone marrow transplantation
Favrot, Marie, Janossy, G., Tidman, N., Blacklock, Hilary, Lopez, Elisa, Bofill, Margarita, ...
Various T cell subsets were characterized by double immunofluorescent staining using monoclonal antibodies (MoAb) in blood, bone marrow (BM) and tissues of 29 patients after allogeneic BM...
Duke, O L, Hobbs, S, Panayi, G S, Poulter, L W, Rasker, J J, Janossy, G
The histochemical demonstration of acid phosphatase (ACP) and adenosine triphosphatase (ATP) has been combined with standard immunofluorescence techniques, using a panel of monoclonal and...
Recognition of a human T-lymphocyte differentiation antigen by an IgM monoclonal antibody.
Bastin, J M, Granger, S, Tidman, N, Janossy, G, McMichael, A J
A monoclonal antibody directed at a determinant on human T cells was produced and characterized. This IgM antibody, MBG6, bound to human peripheral blood T lymphocytes and to medullary thymocytes. It...
Poulter, L W, Duke, O, Hobbs, S, Janossy, G, Panayi, G
A combination of immunochemical staining for HLA-DR antigens and the histochemical demonstration of enzyme activity has been used to identify specific cell populations in the normal and arthritic...
Duke, O, Panayi, G S, Janossy, G, Poulter, L W
We have used monoclonal antibodies of the orthoclone (OKT) series to identify T cell subsets in an immunohistological analysis of the synovial membranes obtained from normal individuals and patients...
Immunological analysis of the skin in graft versus host disease.
Lampert, I A, Janossy, G, Suitters, A J, Bofill, M, Palmer, S, Gordon-Smith, E, ...
Immunohistochemical analysis was performed on skin biopsies from patients suffering from graft versus host disease (GVHD) following bone marrow transplantation for aplastic anaemia and leukaemia....
Enzymes of purine metabolism in human peripheral lymphocyte subpopulations.
Massaia, M, Ma, D D, Sylwestrowicz, T A, Tidman, N, Price, G, Janossy, G, ...
Ecto-5'nucleotidase (5'NT), adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and deoxycytidine (CdR), deoxyguanosine (GdR), deoxyadenosine (AdR) and adenosine (AR) kinases have been...
Poulter, L W, Duke, O, Hobbs, S, Janossy, G, Panayi, G, Seymour, G
Macrophage like cells expressing high concentrations of HLA-DR antigen have been identified in situ within the synovium of patients with rheumatoid arthritis. The characteristics of these cells have...
Crawford, D H, Brickell, P, Tidman, N, McConnell, I, Hoffbrand, A V, Janossy, G
Peripheral blood cells from six patients with acute infectious mononucleosis were studied by two-colour immunofluorescence using antibodies to human T cells, inducer and suppressor-cytotoxic T cell...
Selby, W S, Janossy, G, Goldstein, G, Jewell, D P
T lymphocytes in the normal human intestinal tract have been analysed in tissue sections by a double-marker immunofluorescence technique, combining antiserum to T lymphocyte antigen (HuTLA) with a...
Tidman, N, Janossy, G, Bodger, M, Granger, S, Kung, P C, Goldstein, G
Three new techniques have been introduced for the phenotypic analysis of human thymocytes and T cells. Monoclonal antibodies of different subclasses (i.e. IgG1 and IgG2) were used in various...
Seymour, G J, Greaves, M F, Janossy, G
A method has been developed for identifying human lymphocyte populations in tissue sections using hetero-antisera to T cells and to the B cell associated Ia-like, p28,33 polypeptide complex. The...
Large mononuclear (veiled) cells like 'Ia-like' membrane antigens in human afferent lympn.
Spry, C J, Pflug, A J, Janossy, G, Humphrey, J H
Studies were done to see whether large mononuclear cells in human afferent lymph possess cytoplasmic veils or Ia-like antigens on their surface. Small numbers of veiled cells were seen in lymph from...
Janossy, G, Waxdal, M J, Platts-Mills, T
Purified proteins (Pa-1 and Pa-2) from pokeweed have been compared with commercial pokeweed mitogen (PWM-G) and other mitogens in their ability to stimulate human lymphocytes. With cultures of T and...
Differential expression of MHC class II antigens in chronic B-cell disorders.
Drexler, H G, Gignac, S M, Brenner, M K, Coustan-Smith, E, Janossy, G, Hoffbrand, A V
Cells from the peripheral blood of 22 patients with chronic B-cell disorders were examined for the expression of surface MHC class II antigens. We made use of well-characterized monoclonal antibodies...
CD5-positive B cells after T cell depleted bone marrow transplantation.
Drexler, H G, Brenner, M K, Wimperis, J Z, Gignac, S M, Janossy, G, Prentice, H G, ...
CD5 is an antigen normally found on T cells and on a minority subpopulation of B cells in fetal spleen and tonsil and on the majority of cells in B-chronic lymphocytic leukaemia. Recent reports...
Janossy, G., Greaves, M. F., Doenhoff, M. J., Šnajdr, J.
Although mitogens are frequently used for polyclonal lymphocyte stimulation in both experimental and clinical studies, biological tests of this kind encounter several technical difficulties. The...
Asherson, G. L., Ferluga, J., Janossy, G.
The ability of mouse lymphoid cells to show non-specific cytotoxicity to 51Cr-labelled mastocytoma cells in vitro was assessed by the release of 51Cr.
Doenhoff, M. J., Janossy, G., Greaves, M. F., Gomer, K. J., Snajdr, J.
In the culture and assay systems described here the majority of mouse spleen cells stimulated by soluble concanavalin A and phytohaemagglutinin have been shown to be T cells, whereas bacterial...
Laboratory control values for CD4 and CD8 T lymphocytes. Implications for HIV-1 diagnosis.
Bofill, M, Janossy, G, Lee, C A, MacDonald-Burns, D, Phillips, A N, Sabin, C, ...
With the advent of standard flow cytometric methods using two-colour fluorescence on samples of whole blood, it is possible to establish the ranges of CD3, CD4 and CD8 T lymphocyte subsets in the...
Webster, A, Phillips, A N, Lee, C A, Janossy, G, Kernoff, P B, Griffiths, P D
After a maximum of 11 years (median 8.3 years) from the time of HIV seroconversion, 25 out of 59 (42%) of CMV-seropositive haemophiliacs had progressed to AIDS, as opposed to eight out of 50 (16%)...
Malizia, G, Trejdosiewicz, L K, Wood, G M, Howdle, P D, Janossy, G, Losowsky, M S
Peroral jejunal biopsies were studied by double-label immunofluorescence on cryostat tissue sections from control patients of normal histology and patients with coeliac disease. A panel of monoclonal...
An immunohistological approach to persistent lymphadenopathy and its relevance to AIDS.
Janossy, G, Pinching, A J, Bofill, M, Weber, J, McLaughlin, J E, Ornstein, M, ...
Recent evidence has shown that not only AIDS but also the majority of 'unexplained' persistent, generalized lymphadenopathy (PGL) are related to HTLV-III/LAV infections. The early detection how these...
Human macrophages and T-lymphocyte subsets infiltrating nodules of Onchocerca volvulus.
Parkhouse, R M, Bofill, M, Gomez-Priego, A, Janossy, G
The nature of the lymphoid infiltrate in nodules of Onchocerca volvulus was assessed using monoclonal antibodies to lymphoid cell surface markers. Although B cells were generally absent, T cells were...
T lymphocyte regeneration after transplantation of T cell depleted allogeneic bone marrow
Janossy, G., Prentice, H. G., Ivory, Kamal, Tidman, N., Grundy, Jane, ...
The regeneration of T cell subsets was studied with double immunofluorescence marker methods in 37 patients who received HLA matched T lymphocyte depleted bone marrow transplants (BMT) as part of the...
Lymphocyte activation: I. Response of T and B lymphocytes to phytomitogens
The selectivity of phytomitogens for T (thymus derived/dependent) and B (`bursa-equivalent' dependent/derived) lymphocytes from the mouse spleen has been investigated. Responses of normal spleen cell...
The mitogenic selectivity of four phytomitogens, phytohaemagglutinin (PHA), concanavalin (ConA), lentil mitogen (LM) and pokeweed mitogen (PWM), and a series of heterologous antilymphocyte sera (ALS)...
Lymphocyte activation. III. Binding sites for phytomitogens on lymphocyte subpopulations
Greaves, M. F., Bauminger, S., Janossy, G.
The presence of binding sites for concanavalin A, phytohaemagglutinin and lentil mitogen on the surface of mouse B and T lymphocytes has been investigated in relation to the previously described...
Campbell, D A, Poulter, L W, Janossy, G, Du Bois, R M
Immunohistological analysis using monoclonal antibodies in conjunction with histochemical techniques has been applied to lung biopsy material from patients with cryptogenic fibrosing alveolitis....
Use of CD4 lymphocyte count to predict long-term survival free of AIDS after HIV infection.
Phillips, A. N., Sabin, C. A., Elford, J., Bofill, M., Janossy, G., Lee, C. A.
OBJECTIVE--To estimate the probability of remaining free of AIDS for up to 25 years after infection with HIV by extrapolation of changes in CD4 lymphocyte count. DESIGN--Cohort study of subjects...
Immunohistological analysis of Tac antigen expression in tissues involved by Hodgkin's disease.
Pizzolo, G., Chilosi, M., Semenzato, G., Caligaris-Cappio, F., Fiore-Donati, L., Perona, G., ...
Janossy, G., Thomas, J. A., Pizzolo, G., Granger, S. M., McLaughlin, J., Habeshaw, J. A., ...
Tissue sections of frozen biopsy specimens obtained from normal and hyperplastic human lymphoid tissues, 33 cases of non-Hodgkin lymphomas as well as various forms of immunoregulatory disorders...
Nuclear terminal deoxynucleotidyl transferase in leukaemic infiltrates of testicular tissue.
Thomas, J. A., Janossy, G., Eden, O. B., Bollum, F. J.
Early detection of testicular leukaemia and the identification of residual leukaemic cells in treated patients are important aims in the management of males with acute lymphoblastic leukaemia (ALL)....
Chilosi, M., Pizzolo, G., Fiore-Donati, L., Bofill, M., Janossy, G.
Enzyme histochemical and immunohistological (immuno-fluorescence and -peroxidase) techniques have been routinely used for investigating over 70 normal and pathological bone marrow samples. This...