G. K. Abruzzo

Quantitative PCR Assay To Measure Aspergillus fumigatus Burden in a Murine Model of Disseminated Aspergillosis: Demonstration of Efficacy of Caspofungin Acetate

Bowman, J. C., Abruzzo, G. K., Anderson, J. W., Flattery, A. M., Gill, C. J., Pikounis, V. B., ...

Caspofungin acetate (MK-0991) is an antifungal antibiotic that inhibits the synthesis of 1,3-β-d-glucan, an essential component of the cell wall of several pathogenic fungi. Caspofungin acetate was...

In vitro evaluation of the pneumocandin antifungal agent L-733560, a new water-soluble hybrid of L-705589 and L-731373.

Bartizal, K, Scott, T, Abruzzo, G K, Gill, C J, Pacholok, C, Lynch, L, ...

Lipopeptide L-733560 is a hybrid analog of L-731373 and L-705589. All are water-soluble semisynthetic pneumocandin Bo derivatives. In vitro susceptibility testing of L-705589, L-731373, and L-733560...

Evaluation of water-soluble pneumocandin analogs L-733560, L-705589, and L-731373 with mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis.

Abruzzo, G K, Flattery, A M, Gill, C J, Kong, L, Smith, J G, Krupa, D, ...

The activities of the water-soluble pneumocandin derivatives L-733560, L-705589, and L-731373 were evaluated in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis and were...

New model of oropharyngeal and gastrointestinal colonization by Candida albicans in CD4+ T-cell-deficient mice for evaluation of antifungal agents.

Flattery, A M, Abruzzo, G K, Gill, C J, Smith, J G, Bartizal, K

A new model for the evaluation of antifungal compounds against oropharyngeal and gastrointestinal mucosal colonization by Candida albicans was developed. To simulate the immune deficiency observed in...

In vitro preclinical evaluation studies with the echinocandin antifungal MK-0991 (L-743,872).

Bartizal, K, Gill, C J, Abruzzo, G K, Flattery, A M, Kong, L, Scott, P M, ...

The echinocandin MK-0991, formerly L-743,872, is a water-soluble lipopeptide that has been demonstrated in preclinical studies to have potent activity against Candida spp., Aspergillus fumigatus, and...

Evaluation of the echinocandin antifungal MK-0991 (L-743,872): efficacies in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis.

Abruzzo, G K, Flattery, A M, Gill, C J, Kong, L, Smith, J G, Pikounis, V B, ...

The in vivo activity of the Merck antifungal echinocandin drug candidate MK-0991 (L-743,872) was evaluated in mouse models of disseminated candidiasis, aspergillosis, and cryptococcosis. The...

Identification of the FKS1 gene of Candida albicans as the essential target of 1,3-beta-D-glucan synthase inhibitors.

Douglas, C M, D'Ippolito, J A, Shei, G J, Meinz, M, Onishi, J, Marrinan, J A, ...

Pneumocandins and echinocandins are fungicidal antibiotics, currently in clinical development, that inhibit 1,3-beta-D-glucan synthase (GS) in several human fungal pathogens. We have identified a...

PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence.

Smith, J G, Kong, L, Abruzzo, G K, Gill, C J, Flattery, A M, Scott, P M, ...

Many animal models of Helicobacter infection have been described, including infection in rhesus monkeys, ferrets, gnotobiotic piglets, and mice. These animal models utilize a combination of detection...

A sensitive and specific PCR method to detect Helicobacter felis in a conventional mouse model.

Kong, L, Smith, J G, Bramhill, D, Abruzzo, G K, Bonfiglio, C, Cioffe, C, ...

Although many detection methods have been used to determine Helicobacter colonization in small animal models, the sensitivity and specificity of these detection methods are limited. To improve the...

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells.

Abruzzo, G K, Giltinan, D M, Capizzi, T P, Fromtling, R A

The luminol-enhanced chemiluminescence (CL) assay is a measure of the early events of phagocytosis, leukocyte activation, and immune cell interactions. Reduction in the CL response of immune cells...

Design and analysis considerations in evaluating the chemiluminescence response of mouse spleen cells.

Giltinan, D M, Capizzi, T P, Abruzzo, G K, Fromtling, R A

Chemiluminescence is the result of the respiratory burst generated by phagocytic cells after stimulation by antigen. The measurement of chemiluminescence represents a sensitive means for detecting...

Candida tropicalis infection in normal, diabetic, and neutropenic mice.

Fromtling, R A, Abruzzo, G K, Giltinan, D M

Opportunistic infections caused by Candida tropicalis have been noted with increasing frequency in compromised patients. The pathogenicity of three isolates of C. tropicalis was studied in normal...

Quantitative PCR Assay To Measure Aspergillus fumigatus Burden in a Murine Model of Disseminated Aspergillosis: Demonstration of Efficacy of Caspofungin Acetate

Bowman, J. C., Abruzzo, G. K., Anderson, J. W., Flattery, A. M., Gill, C. J., Pikounis, V. B., ...

Caspofungin acetate (MK-0991) is an antifungal antibiotic that inhibits the synthesis of 1,3-β-d-glucan, an essential component of the cell wall of several pathogenic fungi. Caspofungin acetate was...

In vitro evaluation of the pneumocandin antifungal agent L-733560, a new water-soluble hybrid of L-705589 and L-731373.

Bartizal, K, Scott, T, Abruzzo, G K, Gill, C J, Pacholok, C, Lynch, L, ...

Lipopeptide L-733560 is a hybrid analog of L-731373 and L-705589. All are water-soluble semisynthetic pneumocandin Bo derivatives. In vitro susceptibility testing of L-705589, L-731373, and L-733560...

Evaluation of water-soluble pneumocandin analogs L-733560, L-705589, and L-731373 with mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis.

Abruzzo, G K, Flattery, A M, Gill, C J, Kong, L, Smith, J G, Krupa, D, ...

The activities of the water-soluble pneumocandin derivatives L-733560, L-705589, and L-731373 were evaluated in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis and were...

New model of oropharyngeal and gastrointestinal colonization by Candida albicans in CD4+ T-cell-deficient mice for evaluation of antifungal agents.

Flattery, A M, Abruzzo, G K, Gill, C J, Smith, J G, Bartizal, K

A new model for the evaluation of antifungal compounds against oropharyngeal and gastrointestinal mucosal colonization by Candida albicans was developed. To simulate the immune deficiency observed in...

In vitro preclinical evaluation studies with the echinocandin antifungal MK-0991 (L-743,872).

Bartizal, K, Gill, C J, Abruzzo, G K, Flattery, A M, Kong, L, Scott, P M, ...

The echinocandin MK-0991, formerly L-743,872, is a water-soluble lipopeptide that has been demonstrated in preclinical studies to have potent activity against Candida spp., Aspergillus fumigatus, and...

Evaluation of the echinocandin antifungal MK-0991 (L-743,872): efficacies in mouse models of disseminated aspergillosis, candidiasis, and cryptococcosis.

Abruzzo, G K, Flattery, A M, Gill, C J, Kong, L, Smith, J G, Pikounis, V B, ...

The in vivo activity of the Merck antifungal echinocandin drug candidate MK-0991 (L-743,872) was evaluated in mouse models of disseminated candidiasis, aspergillosis, and cryptococcosis. The...

Identification of the FKS1 gene of Candida albicans as the essential target of 1,3-beta-D-glucan synthase inhibitors.

Douglas, C M, D'Ippolito, J A, Shei, G J, Meinz, M, Onishi, J, Marrinan, J A, ...

Pneumocandins and echinocandins are fungicidal antibiotics, currently in clinical development, that inhibit 1,3-beta-D-glucan synthase (GS) in several human fungal pathogens. We have identified a...

PCR detection of colonization by Helicobacter pylori in conventional, euthymic mice based on the 16S ribosomal gene sequence.

Smith, J G, Kong, L, Abruzzo, G K, Gill, C J, Flattery, A M, Scott, P M, ...

Many animal models of Helicobacter infection have been described, including infection in rhesus monkeys, ferrets, gnotobiotic piglets, and mice. These animal models utilize a combination of detection...

A sensitive and specific PCR method to detect Helicobacter felis in a conventional mouse model.

Kong, L, Smith, J G, Bramhill, D, Abruzzo, G K, Bonfiglio, C, Cioffe, C, ...

Although many detection methods have been used to determine Helicobacter colonization in small animal models, the sensitivity and specificity of these detection methods are limited. To improve the...

Influence of six antifungal agents on the chemiluminescence response of mouse spleen cells.

Abruzzo, G K, Giltinan, D M, Capizzi, T P, Fromtling, R A

The luminol-enhanced chemiluminescence (CL) assay is a measure of the early events of phagocytosis, leukocyte activation, and immune cell interactions. Reduction in the CL response of immune cells...

Design and analysis considerations in evaluating the chemiluminescence response of mouse spleen cells.

Giltinan, D M, Capizzi, T P, Abruzzo, G K, Fromtling, R A

Chemiluminescence is the result of the respiratory burst generated by phagocytic cells after stimulation by antigen. The measurement of chemiluminescence represents a sensitive means for detecting...

Candida tropicalis infection in normal, diabetic, and neutropenic mice.

Fromtling, R A, Abruzzo, G K, Giltinan, D M

Opportunistic infections caused by Candida tropicalis have been noted with increasing frequency in compromised patients. The pathogenicity of three isolates of C. tropicalis was studied in normal...

Efficacy of Caspofungin against Aspergillus flavus, Aspergillus terreus, and Aspergillus nidulans▿

Bowman, J. C., Abruzzo, G. K., Flattery, A. M., Gill, C. J., Hickey, E. J., Hsu, M. J., ...

The echinocandin caspofungin is a potent inhibitor of the activity of 1,3-β-d-glucan synthase from Aspergillus flavus, Aspergillus terreus, and Aspergillus nidulans. In murine models of disseminated...