G. K. Andrews

Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation.

Paria, B C, Das, S K, Andrews, G K, Dey, S K

The delayed implantation model was used to study epidermal growth factor receptor(s) (EGF-R) in the mouse blastocyst. Delayed implantation and blastocyst dormancy were induced by ovariectomy on day 4...

Antisense c-myc effects on preimplantation mouse embryo development.

Paria, B C, Dey, S K, Andrews, G K

Antisense DNA inhibition of gene expression was explored as an approach toward elucidating mechanisms regulating development of preimplantation mammalian embryos. Specifically, a role for the c-myc...

Participation of upstream stimulator factor (USF) in cadmium-induction of the mouse metallothionein-I gene.

Li, Q, Hu, N, Daggett, M A, Chu, W A, Bittel, D, Johnson, J A, ...

The roles of the bHLH-Zip protein, upstream stimulatory factor (USF), in mouse metallothionein-I (MT-I) gene expression were examined. The promoter contains a putative USF binding site which overlaps...

Reversible activation of mouse metal response element-binding transcription factor 1 DNA binding involves zinc interaction with the zinc finger domain.

Dalton, T P, Bittel, D, Andrews, G K

The DNA-binding activity of the Zn finger protein metal response element-binding transcription factor 1 (MTF-1) was rapidly induced both in vivo in mouse Hepa cells, canine MDCK, and human HeLa cells...

Butyrate selectively activates the metallothionein gene in teratocarcinoma cells and induces hypersensitivity to metal induction.

Andrews, G K, Adamson, E D

The expression of metallothionein genes (MT-I and MT-II) was shown to be enhanced within 2 h of addition of 2.5-5 mM sodium butyrate to cultures of teratocarcinoma cells. Both undifferentiated stem...

Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA.

Wei, D Y, Andrews, G K

A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I...

The heat shock response in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene.

Andrews, G K, Harding, M A, Calvet, J P, Adamson, E D

Several known inducers of the heat shock response (heat stress, arsenite, and heavy metals) were shown to cause a significant elevation of c-fos mRNA in HeLa cells. Heat stress resulted in a time-...

Transcriptional induction of the mouse metallothionein-I gene in hydrogen peroxide-treated Hepa cells involves a composite major late transcription factor/antioxidant response element and metal response promoter elements.

Dalton, T, Palmiter, R D, Andrews, G K

Synthesis of metallothionein-I (MT-I) and heme oxygenase mRNAs is rapidly and transiently induced by H2O2 in mouse hepatoma cells (Hepa) and this effect is blocked by catalase. Menadione, which...

Tissue specificity of alpha-fetoprotein messenger RNA expression during mouse embryogenesis.

Dziadek, M A, Andrews, G K

Alpha-fetoprotein (AFP) is a synthetic product of only the visceral yolk sac endoderm and fetal liver during mouse embryogenesis. To examine the involvement of transcriptional and...

Induction of hepatic metallothioneins determined at isoprotein and messenger RNA levels in glucocorticoid-treated rats.

Lehman-McKeeman, L D, Andrews, G K, Klaassen, C D

Induction of metallothionein-I (MT-I) and metallothionein-II (MT-II) by glucocorticoids was determined by h.p.l.c. analysis of proteins and Northern-blot analysis of MT mRNAs. Rats were injected with...

Studies on the high-mobility-group non-histone proteins from hen oviduct.

Teng, C S, Andrews, G K, Teng, C T

Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, -2, -3, -14 and -17, which are equivalent to the classification of calf thymus HMG proteins. Hen...

Studies on sex-organ development. Changes in the oestrogenic response of the chick Müllerian duct as measured by chromatin template and ribonucleic acid initiation capacity.

Andrews, G K, Teng, C S

Assays of transcription in vitro, with Escherichia coli RNA polymerase or wheat-germ RNA polymerase II, were used to characterize chromatin templates isolated from the left Müllerian duct of the...

Studies on sex-organ development. Prenatal effect of oestrogenic hormone on tubular-gland cell morphogenesis and ovalbumin-gene expression in the chick Müllerian duct.

Andrews, G K, Teng, C S

The effects of diethylstilboestrol on morphogenesis and cyto-differentiation of the chick-embryo left Müllerian duct were examined. Embryos were treated at different stages of development with...

Identification of a signal transducer and activator of transcription (STAT) binding site in the mouse metallothionein-I promoter involved in interleukin-6-induced gene expression.

Lee, D K, Carrasco, J, Hidalgo, J, Andrews, G K

Mechanisms of regulation of mouse metallothionein (MT)-I gene expression in response to bacterial endotoxin-lipopolysaccharide (LPS) were examined. Northern blot analysis of hepatic MT-I mRNA in...

Analysis of the effects of overexpression of metallothionein-I in transgenic mice on the reproductive toxicology of cadmium.

Dalton, T, Fu, K, Enders, G C, Palmiter, R D, Andrews, G K

Exposure to low levels of cadmium reduces fertility. In male mice spermatogenesis is highly sensitive to cadmium, whereas in females the peri-implantation period of pregnancy is sensitive. To examine...

Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation.

Paria, B C, Das, S K, Andrews, G K, Dey, S K

The delayed implantation model was used to study epidermal growth factor receptor(s) (EGF-R) in the mouse blastocyst. Delayed implantation and blastocyst dormancy were induced by ovariectomy on day 4...

Antisense c-myc effects on preimplantation mouse embryo development.

Paria, B C, Dey, S K, Andrews, G K

Antisense DNA inhibition of gene expression was explored as an approach toward elucidating mechanisms regulating development of preimplantation mammalian embryos. Specifically, a role for the c-myc...

Participation of upstream stimulator factor (USF) in cadmium-induction of the mouse metallothionein-I gene.

Li, Q, Hu, N, Daggett, M A, Chu, W A, Bittel, D, Johnson, J A, ...

The roles of the bHLH-Zip protein, upstream stimulatory factor (USF), in mouse metallothionein-I (MT-I) gene expression were examined. The promoter contains a putative USF binding site which overlaps...

Reversible activation of mouse metal response element-binding transcription factor 1 DNA binding involves zinc interaction with the zinc finger domain.

Dalton, T P, Bittel, D, Andrews, G K

The DNA-binding activity of the Zn finger protein metal response element-binding transcription factor 1 (MTF-1) was rapidly induced both in vivo in mouse Hepa cells, canine MDCK, and human HeLa cells...

Butyrate selectively activates the metallothionein gene in teratocarcinoma cells and induces hypersensitivity to metal induction.

Andrews, G K, Adamson, E D

The expression of metallothionein genes (MT-I and MT-II) was shown to be enhanced within 2 h of addition of 2.5-5 mM sodium butyrate to cultures of teratocarcinoma cells. Both undifferentiated stem...

Molecular cloning of chicken metallothionein. Deduction of the complete amino acid sequence and analysis of expression using cloned cDNA.

Wei, D Y, Andrews, G K

A cDNA library was constructed using RNA isolated from the livers of chickens which had been treated with zinc. This library was screened with a RNA probe complementary to mouse metallothionein-I...

The heat shock response in HeLa cells is accompanied by elevated expression of the c-fos proto-oncogene.

Andrews, G K, Harding, M A, Calvet, J P, Adamson, E D

Several known inducers of the heat shock response (heat stress, arsenite, and heavy metals) were shown to cause a significant elevation of c-fos mRNA in HeLa cells. Heat stress resulted in a time-...

Transcriptional induction of the mouse metallothionein-I gene in hydrogen peroxide-treated Hepa cells involves a composite major late transcription factor/antioxidant response element and metal response promoter elements.

Dalton, T, Palmiter, R D, Andrews, G K

Synthesis of metallothionein-I (MT-I) and heme oxygenase mRNAs is rapidly and transiently induced by H2O2 in mouse hepatoma cells (Hepa) and this effect is blocked by catalase. Menadione, which...

Tissue specificity of alpha-fetoprotein messenger RNA expression during mouse embryogenesis.

Dziadek, M A, Andrews, G K

Alpha-fetoprotein (AFP) is a synthetic product of only the visceral yolk sac endoderm and fetal liver during mouse embryogenesis. To examine the involvement of transcriptional and...

Induction of hepatic metallothioneins determined at isoprotein and messenger RNA levels in glucocorticoid-treated rats.

Lehman-McKeeman, L D, Andrews, G K, Klaassen, C D

Induction of metallothionein-I (MT-I) and metallothionein-II (MT-II) by glucocorticoids was determined by h.p.l.c. analysis of proteins and Northern-blot analysis of MT mRNAs. Rats were injected with...

Studies on the high-mobility-group non-histone proteins from hen oviduct.

Teng, C S, Andrews, G K, Teng, C T

Nuclear high-mobility-group (HMG) proteins were isolated from hen oviduct. These were proteins HMG-1, -2, -3, -14 and -17, which are equivalent to the classification of calf thymus HMG proteins. Hen...

Studies on sex-organ development. Changes in the oestrogenic response of the chick Müllerian duct as measured by chromatin template and ribonucleic acid initiation capacity.

Andrews, G K, Teng, C S

Assays of transcription in vitro, with Escherichia coli RNA polymerase or wheat-germ RNA polymerase II, were used to characterize chromatin templates isolated from the left Müllerian duct of the...

Studies on sex-organ development. Prenatal effect of oestrogenic hormone on tubular-gland cell morphogenesis and ovalbumin-gene expression in the chick Müllerian duct.

Andrews, G K, Teng, C S

The effects of diethylstilboestrol on morphogenesis and cyto-differentiation of the chick-embryo left Müllerian duct were examined. Embryos were treated at different stages of development with...

Identification of a signal transducer and activator of transcription (STAT) binding site in the mouse metallothionein-I promoter involved in interleukin-6-induced gene expression.

Lee, D K, Carrasco, J, Hidalgo, J, Andrews, G K

Mechanisms of regulation of mouse metallothionein (MT)-I gene expression in response to bacterial endotoxin-lipopolysaccharide (LPS) were examined. Northern blot analysis of hepatic MT-I mRNA in...

Analysis of the effects of overexpression of metallothionein-I in transgenic mice on the reproductive toxicology of cadmium.

Dalton, T, Fu, K, Enders, G C, Palmiter, R D, Andrews, G K

Exposure to low levels of cadmium reduces fertility. In male mice spermatogenesis is highly sensitive to cadmium, whereas in females the peri-implantation period of pregnancy is sensitive. To examine...