Integrating field and numerical modeling methods for applied urban karst hydrogeology (2009)
J. Epting, D. Romanov, P. Huggenberger, G. Kaufmann
Infrastructures constructed on unstable geologic formations are prone to subsidence. Data have been collected in the context of an upgrading project for a highway located beside a river dam...
Integrating field and numerical modeling methods for applied urban karst hydrogeology (2009)
J. Epting, D. Romanov, P. Huggenberger, G. Kaufmann
Infrastructures that are constructed on unstable geologic formations are prone to subsidence. Data have been collected in the context of an upgrading project for a highway located beside a river dam...
Meriggiola, MC, Bremner, WJ, Costantino, A, Bertaccini, A, Morselli-Labate, AM, Huebler, D, ...
Androgen-progestin combinations are promising male contraceptive regimens. Optimization of these regimens includes the development of new progestins with more favorable biological properties. In this...
Carboxymethyl cellulose (CMC) powder formulation of apomorphine was prepared by lyophilization and characterized with respect to the in vitro and intranasal in vivo release of apomorphine in rabbits....
Assembly of simian virus 40 Okazaki pieces from DNA primers is reversibly arrested by ATP depletion.
Nethanel, T, Zlotkin, T, Kaufmann, G
We have previously proposed that DNA polymerase alpha-primase provides short RNA-DNA precursors below 40 nucleotides (DNA primers), several of which assemble into an Okazaki piece after intervening...
Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40.
Agents discriminating between DNA polymerase alpha and DNA polymerases of class delta (polymerase delta or epsilon) were used to characterize steps in the synthesis of the lagging DNA strand of...
An Okazaki piece of simian virus 40 may be synthesized by ligation of shorter precursor chains.
Nethanel, T, Reisfeld, S, Dinter-Gottlieb, G, Kaufmann, G
It is generally accepted that an aphidicolin-sensitive DNA polymerase elongates the eucaryotic RNA primer (iRNA) into a mature Okazaki piece reaching ca. 200 nucleotides. Yet, as shown here, nascent...
Shterman, N, Elroy-Stein, O, Morad, I, Amitsur, M, Kaufmann, G
Anticodon nuclease is a bacterial restriction enzyme directed against tRNA(Lys). We report that anticodon nuclease also cleaves mammalian tRNA(Lys) molecules, with preference and site specificity...
An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.
A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5' end. Searching for the putative iRNA polymerase (primase), we detected in extracts from...
Host transfer RNA cleavage and reunion in T4-infected Escherichia coli CTr5x.
T4 mutants lacking polynucleotide kinase (pnk-) or RNA ligase (rli-) do not grow on E. coli CTr5x. During the abortive infections there accumulate host tRNA fragments that match into two species...
Dinter-Gottlieb, G, Kaufmann, G
To synchronize SV40 replicons, simian cells infected with a tsA mutant were restricted at 40 degrees, to complete ongoing replication and returned to 32 degrees, to activate new replicons in the...
Kaufmann, G, Bar-Shavit, R, DePamphilis, M L
Simian virus 40 replicating DNA was pulse labeled with alpha-32P-dATP using an acellular DNA replication system. Nascent DNA chains of less than 200 nucleotides (Okazaki pieces) were then isolated...
David, M, Borasio, G D, Kaufmann, G
The fate of host tRNAs during T4 bacteriophage infection was investigated with Escherichia coli CTr5x, the only known host strain that is restrictive to RNA ligase and polynucleotide kinase mutants....
Cloning of nascent monkey DNA synthesized early in the cell cycle.
Kaufmann, G, Zannis-Hadjopoulos, M, Martin, R G
To study the structure and complexity of animal cell replication origins, we have isolated and cloned nascent DNA from the onset of S phase as follows: African green monkey kidney cells arrested in...
Zannis-Hadjopoulos, M, Kaufmann, G, Wang, S S, Lechner, R L, Karawya, E, Hesse, J, ...
Twelve clones of monkey DNA obtained by a procedure that enriches 10(3)- to 10(4)-fold for nascent sequences activated early in S phase (G. Kaufmann, M. Zannis-Hadjopoulos, and R. G. Martin, Mol....
Amitsur, M, Morad, I, Kaufmann, G
During phage T4 infection of Escherichia coli strains containing the prr locus the host tRNALys undergoes cleavage-ligation in reactions catalyzed by anticodon nuclease, polynucleotide kinase and RNA...
RNA ligase reaction products in plasmolyzed Escherichia coli cells infected by T4 bacteriophage.
David, M, Vekstein, R, Kaufmann, G
Searching for a physiological role of T4 RNA ligase [polyribonucleotide synthetase (ATP); poly(ribonucleotide):poly(ribonucleotide) ligase (AMP-forming), EC 6.5.1.3] activity, we developed an...
DNA polymerase epsilon may be dispensable for SV40- but not cellular-DNA replication.
Zlotkin, T, Kaufmann, G, Jiang, Y, Lee, M Y, Uitto, L, Syväoja, J, ...
The contributions of DNA polymerases alpha, delta, and epsilon to SV40 and nuclear DNA syntheses were evaluated. Proteins were UV-crosslinked to nascent DNA within replicating chromosomes and the...
Amitsur, M, Levitz, R, Kaufmann, G
Host tRNAs cleaved near the anticodon occur specifically in T4-infected Escherichia coli prr strains which restrict polynucleotide kinase (pnk) or RNA ligase (rli) phage mutants. The cleavage...
HSD restriction-modification proteins partake in latent anticodon nuclease.
Amitsur, M, Morad, I, Chapman-Shimshoni, D, Kaufmann, G
Phage T4-induced anticodon nuclease triggers cleavage-ligation of the host tRNA(Lys). The enzyme is encoded in latent form by the optional Escherichia coli locus prr and is activated by the product...
The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease.
Levitz, R, Chapman, D, Amitsur, M, Green, R, Snyder, L, Kaufmann, G
The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon...
Assembly of simian virus 40 Okazaki pieces from DNA primers is reversibly arrested by ATP depletion.
Nethanel, T, Zlotkin, T, Kaufmann, G
We have previously proposed that DNA polymerase alpha-primase provides short RNA-DNA precursors below 40 nucleotides (DNA primers), several of which assemble into an Okazaki piece after intervening...
Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40.
Agents discriminating between DNA polymerase alpha and DNA polymerases of class delta (polymerase delta or epsilon) were used to characterize steps in the synthesis of the lagging DNA strand of...
An Okazaki piece of simian virus 40 may be synthesized by ligation of shorter precursor chains.
Nethanel, T, Reisfeld, S, Dinter-Gottlieb, G, Kaufmann, G
It is generally accepted that an aphidicolin-sensitive DNA polymerase elongates the eucaryotic RNA primer (iRNA) into a mature Okazaki piece reaching ca. 200 nucleotides. Yet, as shown here, nascent...
Shterman, N, Elroy-Stein, O, Morad, I, Amitsur, M, Kaufmann, G
Anticodon nuclease is a bacterial restriction enzyme directed against tRNA(Lys). We report that anticodon nuclease also cleaves mammalian tRNA(Lys) molecules, with preference and site specificity...
An oligoribonucleotide polymerase from SV40-infected cells with properties of a primase.
A transient decaribonucleotide (iRNA) is covalently linked to nascent eukaryotic DNA chains at their 5' end. Searching for the putative iRNA polymerase (primase), we detected in extracts from...
Host transfer RNA cleavage and reunion in T4-infected Escherichia coli CTr5x.
T4 mutants lacking polynucleotide kinase (pnk-) or RNA ligase (rli-) do not grow on E. coli CTr5x. During the abortive infections there accumulate host tRNA fragments that match into two species...
Dinter-Gottlieb, G, Kaufmann, G
To synchronize SV40 replicons, simian cells infected with a tsA mutant were restricted at 40 degrees, to complete ongoing replication and returned to 32 degrees, to activate new replicons in the...
Kaufmann, G, Bar-Shavit, R, DePamphilis, M L
Simian virus 40 replicating DNA was pulse labeled with alpha-32P-dATP using an acellular DNA replication system. Nascent DNA chains of less than 200 nucleotides (Okazaki pieces) were then isolated...
David, M, Borasio, G D, Kaufmann, G
The fate of host tRNAs during T4 bacteriophage infection was investigated with Escherichia coli CTr5x, the only known host strain that is restrictive to RNA ligase and polynucleotide kinase mutants....
Cloning of nascent monkey DNA synthesized early in the cell cycle.
Kaufmann, G, Zannis-Hadjopoulos, M, Martin, R G
To study the structure and complexity of animal cell replication origins, we have isolated and cloned nascent DNA from the onset of S phase as follows: African green monkey kidney cells arrested in...
Zannis-Hadjopoulos, M, Kaufmann, G, Wang, S S, Lechner, R L, Karawya, E, Hesse, J, ...
Twelve clones of monkey DNA obtained by a procedure that enriches 10(3)- to 10(4)-fold for nascent sequences activated early in S phase (G. Kaufmann, M. Zannis-Hadjopoulos, and R. G. Martin, Mol....
Amitsur, M, Morad, I, Kaufmann, G
During phage T4 infection of Escherichia coli strains containing the prr locus the host tRNALys undergoes cleavage-ligation in reactions catalyzed by anticodon nuclease, polynucleotide kinase and RNA...
RNA ligase reaction products in plasmolyzed Escherichia coli cells infected by T4 bacteriophage.
David, M, Vekstein, R, Kaufmann, G
Searching for a physiological role of T4 RNA ligase [polyribonucleotide synthetase (ATP); poly(ribonucleotide):poly(ribonucleotide) ligase (AMP-forming), EC 6.5.1.3] activity, we developed an...
DNA polymerase epsilon may be dispensable for SV40- but not cellular-DNA replication.
Zlotkin, T, Kaufmann, G, Jiang, Y, Lee, M Y, Uitto, L, Syväoja, J, ...
The contributions of DNA polymerases alpha, delta, and epsilon to SV40 and nuclear DNA syntheses were evaluated. Proteins were UV-crosslinked to nascent DNA within replicating chromosomes and the...
The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease.
Levitz, R, Chapman, D, Amitsur, M, Green, R, Snyder, L, Kaufmann, G
The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon...
Amitsur, M, Levitz, R, Kaufmann, G
Host tRNAs cleaved near the anticodon occur specifically in T4-infected Escherichia coli prr strains which restrict polynucleotide kinase (pnk) or RNA ligase (rli) phage mutants. The cleavage...
HSD restriction-modification proteins partake in latent anticodon nuclease.
Amitsur, M, Morad, I, Chapman-Shimshoni, D, Kaufmann, G
Phage T4-induced anticodon nuclease triggers cleavage-ligation of the host tRNA(Lys). The enzyme is encoded in latent form by the optional Escherichia coli locus prr and is activated by the product...