Krupp, G, Kühne, K, Tamm, S, Klapper, W, Heidorn, K, Rott, A, ...
Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated...
Conrad, F, Hanne, A, Gaur, R K, Krupp, G
For the first time mosaic nucleic acids composed of 50% RNA and 50% DNA can be obtained as transcripts with T7 RNA polymerase. Two NTPs could be replaced simultaneously in a transcription reaction....
A new approach for modification interference studies is presented. It involves the use of phosphorothioates as a handle to analyze any desired base or sugar modification. This method was applied to...
Kleineidam, R G, Pitulle, C, Sproat, B, Krupp, G
RNAse P cleaves pre-tRNAs to liberate 5'-flanks and 5'-matured, 5'-phosphorylated tRNAs. It is not evident if the 2'-hydroxyls of the ribose moieties in the substrate are involved in the reaction. To...
The acceptor stem in pre-tRNAs determines the cleavage specificity of RNase P.
As the result of an unusual RNase P specificity, some special, mature tRNAs have acceptor stems with eight instead of the common seven base pairs. The data from numerous studies suggest that some...
Unusual promoter-independent transcription reactions with bacteriophage RNA polymerases.
Efficient transcription reactions of DNA-dependent RNA polymerases require the presence of a specific promoter sequence. This report shows that in the absence of their cognate promoter, two...
The nucleotide sequence of the 5S rRNA from the archaebacterium Thermoplasma acidophilum.
Luehrsen, K R, Fox, G E, Kilpatrick, M W, Walker, R T, Domdey, H, Krupp, G, ...
The complete nucleotide sequence of the 5S ribosomal RNA isolated from the archaebacterium Thermoplasma acidophilum has been determined. The sequence is: pG...
Feng, Y X, Krupp, G, Gross, H J
The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence...
Using end-labelled RNA, significant changes in base specificity of three nucleases have been detected under defined conditions. Staphylococcus aureus nuclease at pH 3.5 without Ca++ cleaves all Pyr-N...
Kahle, D, Wehmeyer, U, Char, S, Krupp, G
Several modified nucleosides were introduced during in vitro RNA synthesis into a pre-tRNA(Ser). The pre-tRNAs were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP...
Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Krupp, G, Cherayil, B, Frendewey, D, Nishikawa, S, Söll, D
RNase P activity from Schizosaccharomyces pombe co-purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments....
Gaur, R K, Hanne, A, Conrad, F, Kahle, D, Krupp, G
The phosphorothioate footprinting technique was applied to the investigation of phosphate moieties in tRNA substrates involved in interactions with M1 RNA, the catalytic subunit of Escherichia coli...
Singh, K K, Parwaresch, R, Krupp, G
In established methods for analyzing ribozyme kinetics, radiolabeled RNA substrates are primarily used. Each data point requires the cumbersome sampling, gel electrophoretic separation, and...
Kahle, D, Wehmeyer, U, Krupp, G
Modified bases were introduced into pre-tRNAs during in vitro RNA synthesis or by chemical modification. These RNAs were used as substrates for the catalytic M1 RNA and the RNase P holoenzyme from...
Krupp, G, Kühne, K, Tamm, S, Klapper, W, Heidorn, K, Rott, A, ...
Human somatic cells have essentially no telomerase activity. Telomerase is linked to tumor genesis and is a valuable marker for malignant growth. Extreme paucity of the enzyme neccessitated...
Conrad, F, Hanne, A, Gaur, R K, Krupp, G
For the first time mosaic nucleic acids composed of 50% RNA and 50% DNA can be obtained as transcripts with T7 RNA polymerase. Two NTPs could be replaced simultaneously in a transcription reaction....
A new approach for modification interference studies is presented. It involves the use of phosphorothioates as a handle to analyze any desired base or sugar modification. This method was applied to...
Kleineidam, R G, Pitulle, C, Sproat, B, Krupp, G
RNAse P cleaves pre-tRNAs to liberate 5'-flanks and 5'-matured, 5'-phosphorylated tRNAs. It is not evident if the 2'-hydroxyls of the ribose moieties in the substrate are involved in the reaction. To...
The acceptor stem in pre-tRNAs determines the cleavage specificity of RNase P.
As the result of an unusual RNase P specificity, some special, mature tRNAs have acceptor stems with eight instead of the common seven base pairs. The data from numerous studies suggest that some...
Unusual promoter-independent transcription reactions with bacteriophage RNA polymerases.
Efficient transcription reactions of DNA-dependent RNA polymerases require the presence of a specific promoter sequence. This report shows that in the absence of their cognate promoter, two...
The nucleotide sequence of the 5S rRNA from the archaebacterium Thermoplasma acidophilum.
Luehrsen, K R, Fox, G E, Kilpatrick, M W, Walker, R T, Domdey, H, Krupp, G, ...
The complete nucleotide sequence of the 5S ribosomal RNA isolated from the archaebacterium Thermoplasma acidophilum has been determined. The sequence is: pG...
Feng, Y X, Krupp, G, Gross, H J
The nucleotide sequence of 5.8S rRNA from the Chinese silkworm Philosamia cynthia ricini has been determined by gel sequencing and mobility shift methods. The complete primary structure is (sequence...
Using end-labelled RNA, significant changes in base specificity of three nucleases have been detected under defined conditions. Staphylococcus aureus nuclease at pH 3.5 without Ca++ cleaves all Pyr-N...
Kahle, D, Wehmeyer, U, Char, S, Krupp, G
Several modified nucleosides were introduced during in vitro RNA synthesis into a pre-tRNA(Ser). The pre-tRNAs were used as substrates for RNase P enzymes. No effects were observed with biotin-8-ATP...
Kahle, D, Wehmeyer, U, Krupp, G
Modified bases were introduced into pre-tRNAs during in vitro RNA synthesis or by chemical modification. These RNAs were used as substrates for the catalytic M1 RNA and the RNase P holoenzyme from...
Two RNA species co-purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Krupp, G, Cherayil, B, Frendewey, D, Nishikawa, S, Söll, D
RNase P activity from Schizosaccharomyces pombe co-purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments....
Gaur, R K, Hanne, A, Conrad, F, Kahle, D, Krupp, G
The phosphorothioate footprinting technique was applied to the investigation of phosphate moieties in tRNA substrates involved in interactions with M1 RNA, the catalytic subunit of Escherichia coli...
Singh, K K, Parwaresch, R, Krupp, G
In established methods for analyzing ribozyme kinetics, radiolabeled RNA substrates are primarily used. Each data point requires the cumbersome sampling, gel electrophoretic separation, and...