Dieckmann, M E, Murphy, G, Meli, A, Drury, L O C
Plasma processes close to SNR shocks result in the amplification of magnetic fields and in the acceleration of electrons, injecting them into the diffusive acceleration mechanism. The acceleration of...
Visser, K, Katchamart, W, Loza, E, Martinez-Lopez, JA, Salliot, C, Trudeau, J, ...
Objectives: To develop evidence-based recommendations for the use of methotrexate in daily clinical practice in rheumatic disorders. Methods: 751 rheumatologists from 17 countries participated in the...
Visser, K, Katchamart, W, Loza, E, Martinez-Lopez, JA, Salliot, C, Trudeau, J, ...
Objectives: To develop evidence-based recommendationsfor the use of methotrexate in daily clinical practicein rheumatic disorders.Methods: 751 rheumatologists from 17 countriesparticipated in the 3E...
Holtgrewe, H. L., et. al. “Benign prostatic hyperplasia: diagnosis and treatment. ” In: (2008)
C. Chatelain, G. Murphy, O. Yoshida, United Kingdom Health
Mebust, W. K. Measuring disease-specific health status in men with benign prostatic hyperplasia. Measurement Committee of the American Urological Association. Med
Visser, K, Katchamart, W, Loza, E, Martinez-Lopez, JA, Salliot, C, Trudeau, J, ...
Objectives: To develop evidence-based recommendations for the use of methotrexate (MTX) in daily clinical practice in rheumatic disorders. Methods: A total of 751 rheumatologists from 17 countries...
A New State Model for Router Management (2008)
S. P. Maj, G. Murphy, G. Kohli
The following papers are part of the ongoing research on state models. The
Visser, K, Katchamart, W, Loza, E, Martinez-Lopez, JA, Salliot, C, Trudeau, J, ...
Objectives: To develop evidence-based recommendations for the use of methotrexate (MTX) in daily clinical practice in rheumatic disorders. Methods: A total of 751 rheumatologists from 17 countries...
Visser, K, Katchamart, W, Loza, E, Martinez-Lopez, JA, Salliot, C, Trudeau, J, ...
Objectives: To develop evidence-based recommendations for the use of methotrexate (MTX) in daily clinical practice in rheumatic disorders. Methods: A total of 751 rheumatologists from 17 countries...
Fractalkine in rheumatoid arthritis: a review to date (2008)
Murphy, G., Caplice, N., Molloy, M.
Rheumatoid arthritis (RA) is characterized by the expansion of the synovium, with infiltration of pro-inflammatory cells, neovascularization and an abundance of pro-inflammatory cytokines resulting...
Thompson, J, Papp, K, Austad, J, Cambazard, F, ...
Robust optimisation of forest transportation networks: a case study (2007)
Forest transportation costs are the major cost component for many forest product supply chains. In order to minimise these costs, many organisations have turned to optimisation models to guide...
Membrane-type 4 matrix metalloproteinase promotes breast cancer growth and metastases (2006)
Chabottaux, Vincent, Sounni, Nor Eddine, Pennington, CJ, English, WR, Blacher, Silvia, ...
Membrane-type matrix metalloproteinases (MT-MMP) constitute a subfamily of six distinct membrane-associated MMPs. Although the contribution of MT1-MMP during different steps of cancer progression has...
Limb, G.A., Matter, K., Murphy, G., Cambrey, A.D., Bishop, P.N., Morris, G.E., ...
Since the first description of matrix metalloproteinase (MMP)-1 as an interstitial collagenase, great importance has been ascribed to this enzyme in extracellular matrix remodeling during tumoral,...
Optimal bucking of Douglas fir taking into consideration external properties and wood density (2005)
Modeling Ion Thruster Plumes Under Flight and Ground Test Conditions (2004)
Wang, J., Murphy, G., Liewer, P., Brophy, J.
Ion thrusters are valued as a high-specific impulse class of space propulsion system.
(abstract) Modeling the Critical Velocity Ionization Experiment Interaction (2004)
Wang, J., Murphy, G., Biasca, R.
Proper interpretation of critical velocity ionization experiments depends upon understanding the expected results from in-situ or remote sensors. In particular, the 1991 shuttle based CIV experiment...
Space-Hardware Design for Long Life With High Reliability (2004)
Gindorf, T., Miles, R. Jr., Murphy, G.
In 1991, the Cassini Project, NASA's planetary project to place a spacecraft in orbit about Saturn, funded a study at the Jet Propulsion Laboratory (JPL) to identify rules for design and test of...
Single-Event KeV Proton Detection Using a Delta-Doped Charge-Coupled Device (2004)
Nikzad, S., Elliott, S., Croley, D., Cunningham, T., Proniewicz, W., Murphy, G., ...
Using a delta-doped charge-coupled device (CCD), we have demonstrated an order-of-magnitude improvement in the low-energy cutoff for particle detection compared to conventional solid-state detectors.
Sounni, Nor Eddine, Roghi, C, Chabottaux, Vincent, Janssen, M, Munaut, Carine, Maquoi, Erik, ...
Membrane-type 1 matrix metalloproteinase (MT1-MMP) and vascular endothelial growth factor ( VEGF) are two key molecules involved in pericellular proteolysis and cell proliferation during tumor growth...
Murphy, G., Whelan, P.F., McNally, P.J., Tuomi, T., Danilewsky, A.N., Simon, R.
ANKA - Annual Report 2004 S.135-36
Murphy, G., Whelan, P.F., McNally, P.J., Tuomi, T., Simon, R.
10. Int. Conf. on Defects: Recognition, Imaging and Physics in Semiconductors (DRIP X), Blatz-sur-Mer, F, September 29-October 2, 2003
The impact of abuse on men and women with servere learning disabilities and their families (2003)
O'Callaghan, A.C., Murphy, G., Clare, I.C.H.
So far, little systematic attention has been paid to the impact of abuse on men and women with severe learning disabilities. Using the criteria for post-traumatic stress disorder (American...
Holgate, S.T., Davies, D.E., Murphy, G., Powell, R.M., Holloway, J.W.
ADAM 33, the latest of the ADAM proteins to be described, has been identified as a major susceptibility gene in asthma linked to bronchial hyperresponsiveness. It provides an important breakthrough...
THE EUROPEAN PHYSICAL JOURNAL APPLIED PHYSICS (2003)
G. Murphy, P. F. Whelan, P. J. Mcnally, T. Tuomi, R. Simon
The use of neighbourhood intensity comparisons, morphological gradients and Fourier analysis for automated precipitate counting & Pendellösung fringe analysis in X-ray topography
Lee,M. H., Maskos,K., Knäuper,V., Dodds,P., Murphy,G.
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the release of TNF-u, a potent proinflammatory cytokine associated with many chronic debilitating diseases...
The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3 (2002)
Lee,M. H., Verma,V., Maskos,K., Becherer,J. D., Knäuper,V., Dodds,P., ...
Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinases) that comprises an active catalytic domain and several C-terminal domains. We compare the binding...
Lee,M. H., Verma,V., Maskos,K., Nath,D., Knäuper,V., Dodds,P., ...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-terminal domain form (N-TIMP-3) displayed equal binding affinity for tissue necrosis factor-alpha...
Chemical depletion of Arctic ozone in winter 1999/2000 (2002)
Rex, M., Salawitch, R.J., Harris, N.R.P., Braathen, G.O., Schulz, A., Deckelmann, H., ...
The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3 (2002)
Lee, M. H., Verma, V., Maskos, K., Becherer, J. D., Knäuper, V., Dodds, P., ...
Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinases) that comprises an active catalytic domain and several C-terminal domains. We compare the binding...
Lee, M. H., Verma, V., Maskos, K., Nath, D., Knäuper, V., Dodds, P., ...
We previously reported that full-length tissue inhibitor of metallo-proteinase-3 (TIMP-3) and its N-terminal domain form (N-TIMP-3) displayed equal binding affinity for tissue necrosis factor-alpha...
Lee, M. H., Maskos, K., Knäuper, V., Dodds, P., Murphy, G.
Tumor necrosis factor-alpha (TNF-alpha) converting enzyme (TACE/ADAM-17) is responsible for the release of TNF-u, a potent proinflammatory cytokine associated with many chronic debilitating diseases...
Murphy, G, Tarchini, R, Wambutt, R, Pohl, T, ...
The nucleotide sequence was determined for a 340-kb segment of rice chromosome 2, revealing 56 putative protein-coding genes. This represents a density of one gene per 6.1 kb, which is higher than...
Arctic ozone loss in threshold conditions: Match observations in 1997/1998 and 1998/1999 (2001)
Schulz, A., Rex, M., Harris, N. R. P., Braathen, G. O., Reimer, E., Alfier, R., ...
MAYER, K, MURPHY, G, TARCHINI, R, WAMBUTT, R, VOLCKAERT, G, POHL, T, ...
Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana (2000)
Kaneko, T, Nakamura, Y, Kotani, H, Kato, T, Asamizu, E, ...
The genome of the model plant Arabidopsis thaliana has been sequenced by an international collaboration, The Arabidopsis Genome Initiative. Here we report the complete sequence of chromosome 5. This...
Maquoi, Erik, Frankenne, F, Baramova, E, Munaut, Carine, Sounni, Nor Eddine, Remacle, A, ...
Tissue inhibitor of metalloproteinase 2 (TIMP-2) is required for the membrane type 1 matrix metalloproteinase (MT1-MMP)-dependent activation of pro-MMP-2 on the cell surface. MT1-MMP-bound TIMP-2 has...
Progress in Arabidopsis genome sequencing and functional genomics (2000)
Wambutt, R, Murphy, G, Pohl, T, Dusterhoft, A, Stiekema, W, ...
Arabidopsis thaliana has a relatively small genome of approximately 130 Mb containing about 10% repetitive DNA. Genome sequencing studies reveal a gene-rich genome, predicted to contain approximately...
Ozone loss rates determined with Match: Arctic winters 1997/98 and 1998/99 (2000)
Schulz, A., Rex, M., Harris, N. R. P., Braathen, G. O., Kyrö, E., Reimer, E., ...
Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. (2000)
TABATA, S, KANEKO, T, NAKAMURA, Y, KOTANI, H, KATO, T, ASAMIZU, E, ...
Progress in Arabidopsis genome sequencing and functional genomics. (2000)
WAMBUTT, R, MURPHY, G, VOLCKAERT, G, POHL, T, DUSTERHOFT, A, STIEKEMA, W, ...
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana (1999)
Schüller, C, Wambutt, R, Murphy, G, Pohl, T, ...
The higher plant Arabidopsis thaliana (Arabidopsis) is an important model for identifying plant genes and determining their function. To assist biological investigations and to define chromosome...
Chemical ozone loss in the Arctic winter 1994/95 as determined by the Match technique (1999)
Rex, M., Braathen, G. O., Harris, N. R. P., Reimer, E., Beck, A., ...
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana (1999)
Mayer, K., Schuller, C., Wambutt, R., Murphy, G., Volckaert, G., Pohl, T., ...
Kamgarpour, R., Daniel, R. C. W., Fenwick, D. C., McGuigan, K., Murphy, G.
Episodes of subclinical hypocalcaemia occurred in the first 6 weeks after calving in 6 out of 12 multiparous Friesian cows calving in the winter period and 7 out of 23 calving in the summer in a...
Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana. (1999)
MAYER, K, SCHULLER, C, WAMBUTT, R, MURPHY, G, VOLCKAERT, G, POHL, T, ...
Kamgarpour, R., Daniel, R. C. W., Fenwick, D. C., McGuigan, K., Murphy, G.
Episodes of subclinical hypocalcaemia occurred in the first 6 weeks after calving in 6 out of 12 multiparous Friesian cows calving in the winter period and 7 out of 23 calving in the summer in a...
Two-Phase Impure Steam Flow. (1998)
A device has been developed to remove the prominent impurity carbon dioxide gas from steam. The technique consists of a heat exchanger that condenses some of the impure steam to produce a pure...
Shore-to-Ship Steam Purification Inverse Flash Steam Purifier (IFSTEP) Field Unit Tests. (1998)
Murphy, G., Maga, S., Silbernagel, M.
The Inverse Flash Steam Purifier (IFSTEP), a device to remove noncondensable gases from steam, was developed, tested, and evaluated. IFSTEP provides an alternative to methods that generate pure...
Maquoi, Erik, Noël, Agnès, Frankenne, F, Angliker, H, Murphy, G, Foidart, Jean-Michel
The close correlation observed between matrix metalloproteinase 2 (MMP-2) activation and metastatic progression in various tumors suggests that MMP-2 is a 'master switch' triggering tumor spread....
Thesis (Ph.D.)
Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. (1998)
BEVAN, M, BANCROFT, I, BENT, E, LOVE, K, GOODMAN, H, DEAN, C, ...
Reid, S., Rex, M., Fløisand, I., Stordal, F., Carver, G.D., ...
Jones, T. T. C., Adams, J. M., Ageladarakis, P., AliArshad, S., Alper, B., Altmann, H., ...
Planned experiments in the Joint European Torus [Plasma Physics and Controlled Fusion Research, Proceedings, 13th International Conference, Washington, D.C., 1990 (International Atomic Energy Agency,...
Latest JET results in deuterium and deuterium-tritium plasmas (1997)
Adams, J. M., Ageladarakis, P., Arshad, S. A., Alper, B., Altmann, H., Andrew, P., ...
All major JET systems have been fully commissioned for D-T and the DTE1 series of experiments has started with the D-T fuel mixture and operating conditions foreseen for ITER. In the area of ITER...
Chemical ozone loss in the Arctic winters 1991/92 and 1994/95 (Match) (1996)
Rex, M., Harris, N. R. P., Reimer, E., Beck, A., Alfier, R., ...
The evolution of ozone laminae using the Match approach (1996)
Reid, S.J., Rex, M., Reimer, E., Krüger-Carstensen, R., De Haan, L.L., ...
JET results with the new pumped divertor and implications for ITER (1995)
Adams, J. M., Ageladarakis, P., Alper, B., Altmann, H., Andrew, P., AliArshad, S., ...
This paper presents an overview of results of the 1994/95 experimental campaign on JET with the new pumped divertor and draws implications for ITER in the areas of detached and radiative divertor...
MMP-1 expression in normal tissue and in fos-induced bone tumors. (1995)
Vallon, R., Gack, S., Stanton, H., Tuckermann, J., Schorpp, M., Schmidt, J., ...
Keystone Symp.on Molecular and Cellular Biology, Cancer Cell Invasion and Motility, Tamarron, Colo., February 5-11, 1995
Murphy, G, De Lepeleire, I, Gould, R J, Panebianco, D, ...
MK-383 (L-tyrosine, N-(n-butylsulfonyl)-O-[4-butyl(4-piperidinyl)], monohydrochloride monohydrate) is a potent and specific platelet fibrinogen receptor antagonist that may be useful in preventing...
First Results with the Modified Jet - the Jet Team (1994)
Adams, J. M., Agarici, Y., Ageladarakis, P., Alper, B., Altmann, H., Andrew, P., ...
JET was extensively modified in the 1992/93 shutdown. The new pumped divertor and many new systems were brought into operation early in 1994. Operations have progressed to 4MA plasma current and,...
Comparison of human stromelysin and collagenase by cloning and sequence analysis. (1987)
Whitham, S.E., Murphy, G., Angel, P., Rahmsdorf, H.J., Smith, B.J., Lyons, A., ...
Biochemical Journal, 240(1986) S.913-16
Edwards, D.R., Murphy, G., Reynolds, J.J., Whitham, S.E., Docherty, A.J.P., Angel, P., ...
The EMBO Journal, 6(1987) S.1899-1904
AIRBORNE SPREAD OF MEASLES IN A SUBURBAN ELEMENTARY SCHOOL (1978)
RILEY, E. C., MURPHY, G., RILEY, R. L.
A measles epidemic in a modern suburban elementary school In upstate New York In spring, 1974, is analyzed in terms of a model which provides a basis for apportioning the chance of infection from...
Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
Henney, A M, Wakeley, P R, Davies, M J, Foster, K, Hembry, R, Murphy, G, ...
The stromelysins are members of a family of extracellular matrix metalloproteinases. These enzymes may erode the connective tissue in atherosclerotic plaques, leading to fissuring and acute...
Effects of a decision support system on the diagnostic accuracy of users: a preliminary report.
Elstein, A S, Friedman, C P, Wolf, F M, Murphy, G, Miller, J, Fine, P, ...
OBJECTIVES: To assess the effects of incomplete data upon the output of a computerized diagnostic decision support system (DSS), to assess the effects of using the system upon the diagnostic opinions...
Tissier, A F, Marillonnet, S, Klimyuk, V, Patel, K, Torres, M A, Murphy, G, ...
A new system for insertional mutagenesis based on the maize Enhancer/Suppressor-mutator (En/Spm) element was introduced into Arabidopsis. A single T-DNA construct carried a nonautonomous defective...
Cubo, M T, Economou, A, Murphy, G, Johnston, A W, Downie, J A
A group of four rhi (rhizosphere-expressed) genes from the symbiotic plasmid of Rhizobium leguminosarum biovar viciae has been characterized. Although mutation of the rhi genes does not normally...
Relationship between x-ray exposure and malignant transformation in C3H 10T1/2 cells.
Kennedy, A R, Fox, M, Murphy, G, Little, J B
The appearance of transformed foci after x-irradiation of the C3H 10T1/2 line of murine cells requires extensive proliferation followed by prolonged incubation under conditions of confluence. When...
Iredale, J P, Murphy, G, Hembry, R M, Friedman, S L, Arthur, M J
Hepatic lipocytes play a central role in the pathogenesis of liver fibrosis, both via production of extracellular matrix proteins and through secretion of matrix metalloproteinases. In this study, we...
Edwards, D R, Murphy, G, Reynolds, J J, Whitham, S E, Docherty, A J, Angel, P, ...
Exposure of quiescent MRC-5 human fibroblasts to growth factors such as epidermal growth factor, basic fibroblast growth factor or embryonal carcinoma-derived growth factor resulted in the induction...
Murphy, G, Willenbrock, F, Ward, R V, Cockett, M I, Eaton, D, Docherty, A J
Recombinant 72 kDa gelatinase A and a truncated form lacking the C-terminal domain were shown to be activated by organomercurials and to possess similar activities towards a number of substrates. The...
Atkinson, S J, Ward, R V, Reynolds, J J, Murphy, G
The ability of normal rabbit dermal fibroblasts to degrade films of type IV collagen and gelatin when stimulated by phorbol ester was shown to be dependent on the induction, secretion and activation...
Fosang, A J, Last, K, Knäuper, V, Neame, P J, Murphy, G, Hardingham, T E, ...
The actions of recombinant human fibroblast collagenase (MMP1), purified polymorphonuclear leucocyte collagenase (MMP8) and their N-terminal catalytic domain fragments against cartilage aggrecan and...
Matrix metalloproteinases cleave at two distinct sites on human cartilage link protein.
Nguyen, Q, Murphy, G, Hughes, C E, Mort, J S, Roughley, P J
The actions of human recombinant stromelysins-1 and -2, collagenase, gelatinases A and B and matrilysin on neonatal human proteoglycan aggregates were examined. With the exception of gelatinase B,...
Degradation of glomerular basement membrane by purified mammalian metalloproteinases.
Baricos, W H, Murphy, G, Zhou, Y W, Nguyen, H H, Shah, S V
Neutral metalloproteinases degrade components of the extracellular matrix, including collagen types I-V, fibronectin, laminin and proteoglycan. However, their ability to degrade intact glomerular...
Binding of gelatinases A and B to type-I collagen and other matrix components.
Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G
Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...
Ward, R V, Atkinson, S J, Reynolds, J J, Murphy, G
We report that the isolated C-terminal domain of progelatinase A is inhibitory to the activation of this proenzyme by primary skin fibroblast plasma membranes but is unable to inhibit...
Fosang, A J, Last, K, Neame, P J, Murphy, G, Knäuper, V, Tschesche, H, ...
Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E373-A374 'aggrecanase' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed...
Murphy, G, Ward, R, Hembry, R M, Reynolds, J J, Kühn, K, Tryggvason, K
The metalloproteinase 'gelatinase' stored in the granules of pig polymorphonuclear leucocytes has been purified in the latent form. The enzyme is secreted as an Mr 97,000 proenzyme that can be...
Nguyen, Q, Murphy, G, Roughley, P J, Mort, J S
Cartilage proteoglycan aggregates were subjected to degradation by a metalloproteinase, capable of degrading proteoglycan, released from cartilage in culture. This proteinase was demonstrated to be...
Murphy, G, Koklitis, P, Carne, A F
Recombinant human tissue inhibitor of metalloproteinases (TIMP) forms complexes with high-Mr active recombinant stromelysin that are stable over long periods under physiological conditions....
Cawston, T E, Noble, D N, Murphy, G, Smith, A J, Woodley, C, Hazleman, B
A rapid method is described for the purification of human tissue inhibitor of metalloproteinases (TIMP) from plasma which involves immuno-affinity chromatography and gel filtration. The purified...
Comparison of human stromelysin and collagenase by cloning and sequence analysis.
Whitham, S E, Murphy, G, Angel, P, Rahmsdorf, H J, Smith, B J, Lyons, A, ...
A comparison of the cDNA-derived amino acid sequences of human stromelysin and collagenase with the N-terminal sequences of purified enzymes reveals that these metalloproteinases are highly conserved...
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes.
Murphy, G, Cockett, M I, Stephens, P E, Smith, B J, Docherty, A J
The latent forms of stromelysin and collagenase from human gingival fibroblasts were purified to homogeneity. These latent proenzymes underwent serial small reductions in Mr upon activation by...
Koklitis, P A, Murphy, G, Sutton, C, Angal, S
Recombinant human prostromelysin was purified in a single step using Procion Red-Sepharose chromatography. The purified prostromelysin was self-activated to high-Mr (45,000) and low-Mr (28,000) forms...
Murphy, G, Cockett, M I, Ward, R V, Docherty, A J
The abilities of the matrix metalloproteinases 95 kDa and 72 kDa gelatinases (type IV collagenases), stromelysins-1 and -2 and punctuated metalloproteinase (PUMP) to degrade insoluble elastin, type...
Galloway, W A, Murphy, G, Sandy, J D, Gavrilovic, J, Cawston, T E, Reynolds, J J
A metalloproteinase, 'proteoglycanase', that degrades proteoglycan and insoluble type IV collagen as well as casein was purified to homogeneity from rabbit bone culture medium. The major form of this...
Cawston, T E, Murphy, G, Mercer, E, Galloway, W A, Hazleman, B L, Reynolds, J J
1. Pure rabbit bone metalloproteinase inhibitor (TIMP) bound tightly to pure rabbit bone collagenase with an apparent Kd of 1.4 X 10(-10) M. 2. The molecular weight of the enzyme-inhibitor complex...
Murphy, G, Reynolds, J J, Bretz, U, Baggiolini, M
The separation and further purification of human polymorphonuclear-leucocyte collagenase and gelatinase, using modifications of the method of Cawston & Tyler [(1979) Biochem J. 183, 647-656], are...
The latent collagenase and gelatinase of human polymorphonuclear neutrophil leucocytes.
Murphy, G, Bretz, U, Baggiolini, M, Reynolds, J J
Two metallo-proteinases of human neutrophil leucocytes, collagenase and gelatinase, were studied. Collagenase specifically cleaved native collagen into the TCA and TCB fragments, whereas gelatinase...
Purification of rabbit bone inhibitor of collagenase.
Cawston, T E, Galloway, W A, Mercer, E, Murphy, G, Reynolds, J J
1. Rabbit bones in tissue culture synthesize an inhibitor of collagenase during the first 4 days of culture. 2. The inhibitor was purified by a combination of gel filtration, concanavalin...
Murphy, G, Cawston, T E, Reynolds, J J
1. An inhibitor of collagenase of apparent mol.wt. 28000 was isolated from term human amniotic fluid. 2. It is active against mammalian collagenases from a number of species and tissues as well as...
Murphy, G, Cawston, T E, Galloway, W A, Barnes, M J, Bunning, R A, Mercer, E, ...
Gel-filtration chromatography of culture medium from rabbit bone explants separates three latent metalloproteinases with activities against collagen, proteoglycan and gelatin respectively. The...
Collagenase is a component of the specific granules of human neutrophil leucocytes.
Murphy, G, Reynolds, J J, Bretz, U, Baggiolini, M
Azurophil and specific granules were isolated from human polymorphonuclear neutrophil leucocytes. Collagenase was almost exclusively a component of the specific granules. This finding is in contrast...
Evidence that latent collagenases are enzyme-inhibitor complexes.
Sellers, A, Cartwright, E, Murphy, G, Reynolds, J J
Specific collagenase from the culture media of various rabbit tissues and cells exists in active and latent forms. Latent collagenase is most effectively activated with 4-aminophenylmercuric acetate,...
Proteinase inhibitors of bovine nasal cartilage.
Roughley, P J, Murphy, G, Barrett, A J
Extracts from bovine nasal cartilage with 1 M-guanidinium chloride were fractionated by ultrafiltration. Gel chromatography of the low-molecular-weight material resolved three distinct fractions with...
Buckley, D A, Loughran, G, Murphy, G, Fennelly, C, O'Connor, R
Aims: To study the regulation of type 1 insulin like growth factor receptor (IGF-1R) tyrosine kinase activity using the fission yeast Schizosaccharomyces pombe and a green fluorescent protein (GFP)...
Cowell, S, Knäuper, V, Stewart, M L, D'Ortho, M P, Stanton, H, Hembry, R M, ...
SW1353 chondrosarcoma cells cultured in the presence of interleukin-1, concanavalin A or PMA secreted procollagenase 3 (matrix metalloproteinase-13). The enzyme was detected in the culture medium by...
Fibrillin degradation by matrix metalloproteinases: implications for connective tissue remodelling.
Ashworth, J L, Murphy, G, Rock, M J, Sherratt, M J, Shapiro, S D, Shuttleworth, C A, ...
Fibrillin is the principal structural component of the 10-12 nm diameter elastic microfibrils of the extracellular matrix. We have previously shown that both fibrillin molecules and assembled...
Lafleur, M A, Hollenberg, M D, Atkinson, S J, Knäuper, V, Murphy, G, Edwards, D R
Thrombin, a critical enzyme in the coagulation cascade, has also been associated with angiogenesis and activation of the zymogen form of matrix metalloproteinase-2 (MMP-2 or gelatinase-A). We show...
Ward, R V, Hembry, R M, Reynolds, J J, Murphy, G
Human gingival fibroblasts in culture were shown to secrete a 72 kDa progelatinase, of which a proportion in the medium was found to be complexed with tissue inhibitor of metalloproteinases-2...
Hughes, C, Murphy, G, Hardingham, T E
The action of purified rabbit bone stromelysin was investigated on proteoglycan aggregates from pig laryngeal cartilage. The enzyme caused a rapid fall in viscosity of proteoglycan aggregate solution...
Sise, M J, Shackford, S R, Cruickshank, J C, Murphy, G, Fridlund, P H
Cricothyroidotomy for long-term tracheal access was prospectively studied in 76 critically ill patients. Thirty patients (39%) survived and 46 (61%) died. Mean duration of follow-up computed in all...
Matrix metalloproteinases in the formation of human synovial joint cavities.
Edwards, J C, Wilkinson, L S, Soothill, P, Hembry, R M, Murphy, G, Reynolds, J J
Matrix metalloproteinases (MMPs) have been implicated in tissue remodelling in growth and development. A histochemical study of human fetal limbs was undertaken to assess the presence, and...
Localization of stromelysin gene expression in atherosclerotic plaques by in situ hybridization.
Henney, A M, Wakeley, P R, Davies, M J, Foster, K, Hembry, R, Murphy, G, ...
The stromelysins are members of a family of extracellular matrix metalloproteinases. These enzymes may erode the connective tissue in atherosclerotic plaques, leading to fissuring and acute...
Effects of a decision support system on the diagnostic accuracy of users: a preliminary report.
Elstein, A S, Friedman, C P, Wolf, F M, Murphy, G, Miller, J, Fine, P, ...
OBJECTIVES: To assess the effects of incomplete data upon the output of a computerized diagnostic decision support system (DSS), to assess the effects of using the system upon the diagnostic opinions...
Tissier, A F, Marillonnet, S, Klimyuk, V, Patel, K, Torres, M A, Murphy, G, ...
A new system for insertional mutagenesis based on the maize Enhancer/Suppressor-mutator (En/Spm) element was introduced into Arabidopsis. A single T-DNA construct carried a nonautonomous defective...
Cubo, M T, Economou, A, Murphy, G, Johnston, A W, Downie, J A
A group of four rhi (rhizosphere-expressed) genes from the symbiotic plasmid of Rhizobium leguminosarum biovar viciae has been characterized. Although mutation of the rhi genes does not normally...
Relationship between x-ray exposure and malignant transformation in C3H 10T1/2 cells.
Kennedy, A R, Fox, M, Murphy, G, Little, J B
The appearance of transformed foci after x-irradiation of the C3H 10T1/2 line of murine cells requires extensive proliferation followed by prolonged incubation under conditions of confluence. When...
Iredale, J P, Murphy, G, Hembry, R M, Friedman, S L, Arthur, M J
Hepatic lipocytes play a central role in the pathogenesis of liver fibrosis, both via production of extracellular matrix proteins and through secretion of matrix metalloproteinases. In this study, we...
Edwards, D R, Murphy, G, Reynolds, J J, Whitham, S E, Docherty, A J, Angel, P, ...
Exposure of quiescent MRC-5 human fibroblasts to growth factors such as epidermal growth factor, basic fibroblast growth factor or embryonal carcinoma-derived growth factor resulted in the induction...
Murphy, G, Willenbrock, F, Ward, R V, Cockett, M I, Eaton, D, Docherty, A J
Recombinant 72 kDa gelatinase A and a truncated form lacking the C-terminal domain were shown to be activated by organomercurials and to possess similar activities towards a number of substrates. The...
Atkinson, S J, Ward, R V, Reynolds, J J, Murphy, G
The ability of normal rabbit dermal fibroblasts to degrade films of type IV collagen and gelatin when stimulated by phorbol ester was shown to be dependent on the induction, secretion and activation...
Fosang, A J, Last, K, Knäuper, V, Neame, P J, Murphy, G, Hardingham, T E, ...
The actions of recombinant human fibroblast collagenase (MMP1), purified polymorphonuclear leucocyte collagenase (MMP8) and their N-terminal catalytic domain fragments against cartilage aggrecan and...
Matrix metalloproteinases cleave at two distinct sites on human cartilage link protein.
Nguyen, Q, Murphy, G, Hughes, C E, Mort, J S, Roughley, P J
The actions of human recombinant stromelysins-1 and -2, collagenase, gelatinases A and B and matrilysin on neonatal human proteoglycan aggregates were examined. With the exception of gelatinase B,...
Degradation of glomerular basement membrane by purified mammalian metalloproteinases.
Baricos, W H, Murphy, G, Zhou, Y W, Nguyen, H H, Shah, S V
Neutral metalloproteinases degrade components of the extracellular matrix, including collagen types I-V, fibronectin, laminin and proteoglycan. However, their ability to degrade intact glomerular...
Binding of gelatinases A and B to type-I collagen and other matrix components.
Allan, J A, Docherty, A J, Barker, P J, Huskisson, N S, Reynolds, J J, Murphy, G
Matrix sequestration of matrix metalloproteinases may be important for the facilitation of remodelling events and the migration of cells through the extracellular matrix. Using an ELISA technique we...
Ward, R V, Atkinson, S J, Reynolds, J J, Murphy, G
We report that the isolated C-terminal domain of progelatinase A is inhibitory to the activation of this proenzyme by primary skin fibroblast plasma membranes but is unable to inhibit...
Fosang, A J, Last, K, Neame, P J, Murphy, G, Knäuper, V, Tschesche, H, ...
Native and recombinant neutrophil collagenase (MMP-8) was shown to cleave at the E373-A374 'aggrecanase' site in the interglobular domain of aggrecan. The time course of digestion in vitro showed...
Murphy, G, Ward, R, Hembry, R M, Reynolds, J J, Kühn, K, Tryggvason, K
The metalloproteinase 'gelatinase' stored in the granules of pig polymorphonuclear leucocytes has been purified in the latent form. The enzyme is secreted as an Mr 97,000 proenzyme that can be...
Nguyen, Q, Murphy, G, Roughley, P J, Mort, J S
Cartilage proteoglycan aggregates were subjected to degradation by a metalloproteinase, capable of degrading proteoglycan, released from cartilage in culture. This proteinase was demonstrated to be...
Murphy, G, Koklitis, P, Carne, A F
Recombinant human tissue inhibitor of metalloproteinases (TIMP) forms complexes with high-Mr active recombinant stromelysin that are stable over long periods under physiological conditions....
Cawston, T E, Noble, D N, Murphy, G, Smith, A J, Woodley, C, Hazleman, B
A rapid method is described for the purification of human tissue inhibitor of metalloproteinases (TIMP) from plasma which involves immuno-affinity chromatography and gel filtration. The purified...
Comparison of human stromelysin and collagenase by cloning and sequence analysis.
Whitham, S E, Murphy, G, Angel, P, Rahmsdorf, H J, Smith, B J, Lyons, A, ...
A comparison of the cDNA-derived amino acid sequences of human stromelysin and collagenase with the N-terminal sequences of purified enzymes reveals that these metalloproteinases are highly conserved...
Stromelysin is an activator of procollagenase. A study with natural and recombinant enzymes.
Murphy, G, Cockett, M I, Stephens, P E, Smith, B J, Docherty, A J
The latent forms of stromelysin and collagenase from human gingival fibroblasts were purified to homogeneity. These latent proenzymes underwent serial small reductions in Mr upon activation by...
Koklitis, P A, Murphy, G, Sutton, C, Angal, S
Recombinant human prostromelysin was purified in a single step using Procion Red-Sepharose chromatography. The purified prostromelysin was self-activated to high-Mr (45,000) and low-Mr (28,000) forms...
Murphy, G, Cockett, M I, Ward, R V, Docherty, A J
The abilities of the matrix metalloproteinases 95 kDa and 72 kDa gelatinases (type IV collagenases), stromelysins-1 and -2 and punctuated metalloproteinase (PUMP) to degrade insoluble elastin, type...
Ward, R V, Hembry, R M, Reynolds, J J, Murphy, G
Human gingival fibroblasts in culture were shown to secrete a 72 kDa progelatinase, of which a proportion in the medium was found to be complexed with tissue inhibitor of metalloproteinases-2...
Hughes, C, Murphy, G, Hardingham, T E
The action of purified rabbit bone stromelysin was investigated on proteoglycan aggregates from pig laryngeal cartilage. The enzyme caused a rapid fall in viscosity of proteoglycan aggregate solution...
Galloway, W A, Murphy, G, Sandy, J D, Gavrilovic, J, Cawston, T E, Reynolds, J J
A metalloproteinase, 'proteoglycanase', that degrades proteoglycan and insoluble type IV collagen as well as casein was purified to homogeneity from rabbit bone culture medium. The major form of this...
Cawston, T E, Murphy, G, Mercer, E, Galloway, W A, Hazleman, B L, Reynolds, J J
1. Pure rabbit bone metalloproteinase inhibitor (TIMP) bound tightly to pure rabbit bone collagenase with an apparent Kd of 1.4 X 10(-10) M. 2. The molecular weight of the enzyme-inhibitor complex...
Murphy, G, Reynolds, J J, Bretz, U, Baggiolini, M
The separation and further purification of human polymorphonuclear-leucocyte collagenase and gelatinase, using modifications of the method of Cawston & Tyler [(1979) Biochem J. 183, 647-656], are...
The latent collagenase and gelatinase of human polymorphonuclear neutrophil leucocytes.
Murphy, G, Bretz, U, Baggiolini, M, Reynolds, J J
Two metallo-proteinases of human neutrophil leucocytes, collagenase and gelatinase, were studied. Collagenase specifically cleaved native collagen into the TCA and TCB fragments, whereas gelatinase...
Purification of rabbit bone inhibitor of collagenase.
Cawston, T E, Galloway, W A, Mercer, E, Murphy, G, Reynolds, J J
1. Rabbit bones in tissue culture synthesize an inhibitor of collagenase during the first 4 days of culture. 2. The inhibitor was purified by a combination of gel filtration, concanavalin...
Murphy, G, Cawston, T E, Reynolds, J J
1. An inhibitor of collagenase of apparent mol.wt. 28000 was isolated from term human amniotic fluid. 2. It is active against mammalian collagenases from a number of species and tissues as well as...
Murphy, G, Cawston, T E, Galloway, W A, Barnes, M J, Bunning, R A, Mercer, E, ...
Gel-filtration chromatography of culture medium from rabbit bone explants separates three latent metalloproteinases with activities against collagen, proteoglycan and gelatin respectively. The...
Collagenase is a component of the specific granules of human neutrophil leucocytes.
Murphy, G, Reynolds, J J, Bretz, U, Baggiolini, M
Azurophil and specific granules were isolated from human polymorphonuclear neutrophil leucocytes. Collagenase was almost exclusively a component of the specific granules. This finding is in contrast...
Evidence that latent collagenases are enzyme-inhibitor complexes.
Sellers, A, Cartwright, E, Murphy, G, Reynolds, J J
Specific collagenase from the culture media of various rabbit tissues and cells exists in active and latent forms. Latent collagenase is most effectively activated with 4-aminophenylmercuric acetate,...
Matrix metalloproteinases in the formation of human synovial joint cavities.
Edwards, J C, Wilkinson, L S, Soothill, P, Hembry, R M, Murphy, G, Reynolds, J J
Matrix metalloproteinases (MMPs) have been implicated in tissue remodelling in growth and development. A histochemical study of human fetal limbs was undertaken to assess the presence, and...
Proteinase inhibitors of bovine nasal cartilage.
Roughley, P J, Murphy, G, Barrett, A J
Extracts from bovine nasal cartilage with 1 M-guanidinium chloride were fractionated by ultrafiltration. Gel chromatography of the low-molecular-weight material resolved three distinct fractions with...
Buckley, D A, Loughran, G, Murphy, G, Fennelly, C, O'Connor, R
Aims: To study the regulation of type 1 insulin like growth factor receptor (IGF-1R) tyrosine kinase activity using the fission yeast Schizosaccharomyces pombe and a green fluorescent protein (GFP)...
Cowell, S, Knäuper, V, Stewart, M L, D'Ortho, M P, Stanton, H, Hembry, R M, ...
SW1353 chondrosarcoma cells cultured in the presence of interleukin-1, concanavalin A or PMA secreted procollagenase 3 (matrix metalloproteinase-13). The enzyme was detected in the culture medium by...
Fibrillin degradation by matrix metalloproteinases: implications for connective tissue remodelling.
Ashworth, J L, Murphy, G, Rock, M J, Sherratt, M J, Shapiro, S D, Shuttleworth, C A, ...
Fibrillin is the principal structural component of the 10-12 nm diameter elastic microfibrils of the extracellular matrix. We have previously shown that both fibrillin molecules and assembled...
Lafleur, M A, Hollenberg, M D, Atkinson, S J, Knäuper, V, Murphy, G, Edwards, D R
Thrombin, a critical enzyme in the coagulation cascade, has also been associated with angiogenesis and activation of the zymogen form of matrix metalloproteinase-2 (MMP-2 or gelatinase-A). We show...
Sise, M J, Shackford, S R, Cruickshank, J C, Murphy, G, Fridlund, P H
Cricothyroidotomy for long-term tracheal access was prospectively studied in 76 critically ill patients. Thirty patients (39%) survived and 46 (61%) died. Mean duration of follow-up computed in all...
Distinct T-cell subtypes induced with whole cell and acellular pertussis vaccines in children.
Ryan, M, Murphy, G, Ryan, E, Nilsson, L, Shackley, F, Gothefors, L, ...
Recent clinical trials have demonstrated that new generation acellular pertussis vaccines can confer protection against whooping cough. However, the mechanism of protective immunity against...
Young-Min, S, Beeton, C, Laughton, R, Plumpton, T, Bartram, S, Murphy, G, ...
BACKGROUND—Excess tissue matrix accumulates in systemic sclerosis (SSc), accounting for both visceral and dermal fibrosis. It is suggested that decreased serum levels of matrix metalloproteinases...
Chawla, A., Murphy, G., Donnelly, C., Booth, C. L., Johnson, M., Parry, J. V., ...
A guanidine-based antibody avidity assay for the identification of recently acquired human immunodeficiency virus type 1 (HIV-1) infection was evaluated. The kinetics of maturation of antibody...
Hall, P A, Lemoine, N R, Murphy, G, Dowling, R H
A series of molecular changes are now known to be seen in human pancreatic neoplasia, including the very frequent mutational activation of Kirsten ras oncogene at codon 12, overexpression of the...
Inhibition of human gall bladder mucus synthesis in patients undergoing cholecystectomy.
Rhodes, M, Allen, A, Dowling, R H, Murphy, G, Lennard, T W
Hypersection of gall bladder mucus is associated with gall stone formation in animal models. Aspirin inhibits both mucus synthesis and secretion, prevents gall stone formation in animals and reduces...
Effect of ioglycamide (Biligram) on bile flow and biliary lipid secretion in man.
Bell, G D, Doran, J, Fayadh, M, Murphy, G, Dowling, R H
Twenty-one anicteric patients with a t-tube in situ were studied between the ninth and 11th postoperative days. Eleven patients were given an intravenous infusion of the biliary contrast agent...
Veysey, M, Thomas, L, Mallet, A, Jenkins, P, Besser, G, Wass, J, ...
a Gastroenterology Unit, 5th floor, Thomas Guy House, Guy's Hospital, London SE1 9RT, UK, b Mass Spectrometry Laboratory, St Thomas's Hospital Campus, UMDS, London, UK, c Department of...
Bile acid metabolism by fresh human colonic contents: a comparison of caecal versus faecal samples
Thomas, L, Veysey, M, French, G, Hylemon, P, Murphy, G, Dowling, R
BACKGROUND—Deoxycholic acid (DCA), implicated in the pathogenesis of gall stones and colorectal cancer, is mainly formed by bacterial deconjugation (cholylglycine hydrolase (CGH)) and...
Veysey, M, Malcolm, P, Mallet, A, Jenkins, P, Besser, G, Murphy, G, ...
BACKGROUND—Octreotide inhibits gall bladder emptying and prolongs intestinal transit. This leads to increases in the proportion of deoxycholic acid in, and cholesterol saturation of, gall bladder...
Hembry, R. M., Bagga, M. R., Murphy, G., Henderson, B., Reynolds, J. J.
The distribution of the matrix metalloproteinases, collagenase, stromelysin, gelatinases A and B, and the tissue inhibitor of metalloproteinases in cartilage and synovium removed from rabbits up to...
p53 codon 72 polymorphism and human papillomavirus associated skin cancer
O'Connor, D, Kay, E, Leader, M, Atkins, G, Murphy, G, Mabruk, M
Background/Aims—Non-melanoma skin cancers frequently harbour multiple human papillomavirus (HPV) types. A recent report suggests that a polymorphism of the p53 tumour suppressor gene that results...
Recent trends in HIV and other STIs in the United Kingdom: data to the end of 2002
Brown, A, Sadler, K, Tomkins, S, McGarrigle, C, LaMontagne, D, Goldberg, D, ...
Early treated hypothyroidism: development at 3 years.
Murphy, G, Hulse, J A, Jackson, D, Tyrer, P, Glossop, J, Smith, I, ...
Eighty children from the North West and North East Thames Regional Health Authorities who had been identified as having congenital hypothyroidism either by neonatal screening (76 cases) or by...
Folic acid prescription in pregnancy.
Cupples, M. E., Bradley, T., Murphy, G., Lundy, G.
We resolved to prescribe folic acid supplements for all women who attended this practice during the first twelve weeks of pregnancy. Six months after this decision a prescription was recorded in only...
Murphy, G, Kipgen, D, Dennis, M, Sayers, R
Background: The success of infragenicular revascularisation for lower limb ischaemia is limited by the high proportion of patients without ipsilateral long saphenous vein (LSV) of adequate length or...
Elbow fistulas using autogeneous vein: patency rates and results of revision
Murphy, G, Saunders, R, Metcalfe, M, Nicholson, M
Background: The provision and maintenance of vascular access remains a major cost to end stage renal failure programmes. There are few reports regarding the surgical revision of the failing native...
What are the roles of metalloproteinases in cartilage and bone damage?
A role for metalloproteinases in the pathological destruction in diseases such as rheumatoid arthritis and osteoarthritis, and the irreversible nature of the ensuing cartilage and bone damage, have...
MT1-MMP regulates urothelial cell invasion via transcriptional regulation of Dickkopf-3
Saeb-Parsy, K, Veerakumarasivam, A, Wallard, M J, Thorne, N, Kawano, Y, Murphy, G, ...
Membrane type-1 matrix metalloproteinase (MT1-MMP) is a zinc-binding endopeptidase, which plays a crucial role in tumour growth, invasion and metastasis. We have shown previously that MT1-MMP has...
Comprehensive profiling and localisation of the matrix metalloproteinases in urothelial carcinoma
Wallard, M J, Pennington, C J, Veerakumarasivam, A, Burtt, G, Mills, I G, Warren, A, ...
The matrix metalloproteinases (MMPs) are endopeptidases which break down the extracellular matrix and regulate cytokine and growth factor activity. Several MMPs have been implicated in the promotion...
Krubasik, D, Iyer, N G, English, W R, Ahmed, A A, Vias, M, Roskelley, C, ...
p300 is a transcriptional cofactor and prototype histone acetyltransferase involved in regulating multiple cellular processes. We generated p300 deficient (p300−) cells from the colon carcinoma...
Quantitation of TIMP-1 in plasma of healthy blood donors and patients with advanced cancer
Holten-Andersen, M N, Murphy, G, Nielsen, H J, Pedersen, A N, Christensen, I J, Høyer-Hansen, G, ...
Baker, A H, George, S J, Zaltsman, A B, Murphy, G, Newby, A C
Dysregulation of matrix degrading metalloproteinase enzymes (MMPs) leads to increased extracellular matrix turnover, a key event in the local invasion and metastasis of many tumours. The tissue...