Trichothecenes Mycotoxin Studies (2006)
Buchi, G. H., Marletta, M. A., Newberne, P. M., Rogers, A. E., Roush, W. R., Wogan, G. N.
The purposes of the research supported under this contract are to determine the toxicity of two members of the tricothecene mycotoxins, (anguidine, (das) and nivalenol) including their gross and...
Migration of Substances from Flexible Containers for Heat Processed Foods. (1998)
Thirteen different flexible packaging materials, potentially suitable for packaging of heat processed foods, were investigated with respect to the migration of their components during heat...
Modulation of c-myc Gene Expression in Rat Livers by Aflatoxin B1 Exposure and Age (1993)
LARSON, P. S., MCMAHON, G., WOGAN, G. N.
A single dose of aflatoxin B1 (AFB1) caused a rapid and transient induction in c-myc mRNA levels in livers derived from adult male Fischer 344 rats. The inducibility of c-myc expression by AFB1...
Quantitative analysis of aflatoxin-albumin adducts (1993)
Sheabar, F.Z., Groopman, J.D., Wogan, G.N.
Quantitative exposure assessments made using biologically relevant markers will facilitate epidemiological studies of risk from environmental carcinogens. Blood proteins are readily accessible...
Croy, R.G., Nixon, J.E., Sinnhuber, R.O., Wogan, G.N.
The formation of covalent aflatoxin-DNA adducts has been studied in embryo and yearling stages of the rainbow trout (Salmo gairdneri). A linear relationship was found between the amount of aflatoxin...
Gal, A, Tamir, S, Tannenbaum, S R, Wogan, G N
SJL mice spontaneously develop pre-B-cell lymphoma that we hypothesized might stimulate macrophages to produce nitric oxide (NO.). Transplantation of an aggressive lymphoma (RcsX) was used to induce...
Adduct detection by acylation with [35S]methionine: analysis of DNA adducts of 4-aminobiphenyl.
Sheabar, F Z, Morningstar, M L, Wogan, G N
Reaction of synthetic N-(2'-deoxyguanosin-8-yl)-4-aminobiphenyl (dGuo-8-ABP) with t-butoxycarbonyl-L-[35S]methionine, N-hydroxysuccinimidyl ester (35S-labeled TBM-NHS), under optimized conditions...
Sequence alterations in the exon 1 region of the rat c-Ki-ras gene were studied in DNA isolated from aflatoxin B1 (AFB1)-induced rat liver carcinomas and precursor lesions appearing 56 weeks after...
Soman, N R, Correa, P, Ruiz, B A, Wogan, G N
The TPR-MET oncogenic rearrangement was originally observed in an in vitro transformed human osteosarcoma cell line. Recently, we detected the expression of this rearrangement at very low levels in...
Soman, N R, Wogan, G N, Rhim, J S
Activation of the MET protooncogene by a rearrangement involving the fusion of TPR and MET specific gene sequences has been observed in a human osteosarcoma cell line (HOS) treated in vitro with...
Mutations in c-Ki-ras oncogenes in diseased livers of winter flounder from Boston Harbor.
McMahon, G, Huber, L J, Moore, M J, Stegeman, J J, Wogan, G N
Livers of a natural population of winter flounder from a contaminated site in Boston Harbor were examined for the presence of oncogenes by transfection of DNA into NIH 3T3 mouse fibroblasts. Tissues...
Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors.
McMahon, G, Davis, E F, Huber, L J, Kim, Y, Wogan, G N
c-Ki-ras and N-ras oncogenes have been characterized in aflatoxin B1-induced hepatocellular carcinomas. Detection of different protooncogene and oncogene sequences and estimation of their frequency...
Demain, A. L., Hunt, N. A., Malik, V., Kobbe, B., Hawkins, H., Matsuo, K., ...
This report describes an improved procedure for production of cytochalasin E and tremorgens by solid substrate, agitated fermentation of Aspergillus clavatus on pearled barley.
Production and antibacterial activity of malforming C, a toxic metabolite of Aspergillus niger.
Kobbe, B, Cushman, M, Wogan, G N, Demain, A L
The production of the new mycotoxin malformin C by a solid substrate fermentation is described. Malformin C is highly toxic (mean lethal dose = 0.9 mg/kg) and exerts antibacterial activity against a...
Mollicellins: mutagenic and antibacterial mycotoxins.
Stark, A A, Kobbe, B, Matsuo, D, Büchi, G, Wogan, G N, Demain, A L
Eight mollicellins (depsidones) were assayed for mutagenicity and antibacterial activity in Salmonella/microsome tests involving histidine reversion and forward mutation to 8-azaguanine resistance....
McMahon, G, Davis, E, Wogan, G N
Activated c-Ki-ras genes in liver tumors from rats exposed to the potent hepatocarcinogen aflatoxin B1 were analyzed to determine the nature of their activation by characterization of two c-Ki-ras...
Monoclonal antibody to aflatoxin B1-modified DNA detected by enzyme immunoassay.
Haugen, A, Groopman, J D, Hsu, I C, Goodrich, G R, Wogan, G N, Harris, C C
Monoclonal antibodies were obtained after fusion of mouse P3 X 63 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with aflatoxin B1-adducted DNA complexed with...
Quantitation of aflatoxin B1 adduction within the ribosomal RNA gene sequences of rat liver DNA.
The in vivo formation of covalent aflatoxin B1 (AFB1)-DNA adducts within the rRNA gene sequences of nuclear DNA has been studied in AFB1-treated rats. Liver nuclear DNA, enriched in ribosomal DNA...
Pezzuto, J M, Lau, P P, Luh, Y, Moore, P D, Wogan, G N, Hecht, S M
3-Amino-1-methyl-5H-pyrido[4,3-b]indole, previously reported to be a component of tryptophan pyrolysates, is an intensely mutagenic compound requiring microsomal activation for expression of...
In vitro reactions of aflatoxin B1-adducted DNA.
Groopman, J D, Croy, R G, Wogan, G N
The chemical stability of aflatoxin B1 bound to calf thymus DNA was studied over a 48-hour exposure to phosphate buffers at pH 6.8-8.0 (37 degrees C). During this time, aliquots of the aflatoxin...
Essigmann, J M, Barker, L J, Fowler, K W, Francisco, M A, Reinhold, V N, Wogan, G N
Sterigmatocystin (ST), a potent hepatocarcinogen, was covalently bound to calf thymus DNA by incubation in the presence of phenobarbital-induced rat liver microsomes. Acid hydrolysis of ST-modified...
Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.
Stark, A A, Essigmann, J M, Demain, A L, Skopek, T R, Wogan, G N
Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to...
Identification of an activated c-Ki-ras oncogene in rat liver tumors induced by aflatoxin B1.
McMahon, G, Hanson, L, Lee, J J, Wogan, G N
Weanling male Fischer rats were administered 40 intraperitoneal injections of aflatoxin B1 (25 micrograms per animal per day) over a 2-month period. This chronic dosing regimen resulted in the...
Groopman, J D, Donahue, P R, Zhu, J Q, Chen, J S, Wogan, G N
A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and...
Groopman, J D, Trudel, L J, Donahue, P R, Marshak-Rothstein, A, Wogan, G N
Monoclonal antibodies specific for aflatoxin B1, aflatoxin B2, aflatoxin M1, and the major aflatoxin-DNA adducts were obtained following fusion of mouse SP-2 myeloma cells with spleen cells of mice...
Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver.
Croy, R G, Essigmann, J M, Reinhold, V N, Wogan, G N
The products of in vivo covalent binding of activated aflatoxin B1 (AFB1) to DNA have been investigated in rats. The principal covalent product formed in liver DNA of rats treated with AFB1 has been...
Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro.
Essigmann, J M, Croy, R G, Nadzan, A M, Busby, W F, Reinhold, V N, Büchi, G, ...
The covalent binding of the hepatocarcinogen aflatoxin B1 by rat liver microsomes to calf thymus DNA resulted in a binding level equal to one aflatoxin residue per 60 DNA nucleotides. An aflatoxin...
Molecular epidemiology is increasingly being applied in studies of cancer risks derived from exposure to environmental carcinogens of both endogenous and exogenous origins. Analytical methods have...
Groopman, J D, Wild, C P, Hasler, J, Junshi, C, Wogan, G N, Kensler, T W
Human epidemiology and experimental animal data have provided the statistical association and biological information necessary to propose that aflatoxins are risk factors for human liver cancer. As...
Markers of exposure to carcinogens.
Methods have been developed for the detection of exposure to carcinogens and other DNA damaging agents in experimental animals and man through the detection of carcinogens or their metabolic...
Chemical and biochemical dosimetry of exposure to genotoxic chemicals.
Epidemiologic studies designed to evaluate the health significance of environmental chemicals are compromised by the lack of quantitative exposure data for individuals in exposed populations....
Gal, A, Tamir, S, Tannenbaum, S R, Wogan, G N
SJL mice spontaneously develop pre-B-cell lymphoma that we hypothesized might stimulate macrophages to produce nitric oxide (NO.). Transplantation of an aggressive lymphoma (RcsX) was used to induce...
Adduct detection by acylation with [35S]methionine: analysis of DNA adducts of 4-aminobiphenyl.
Sheabar, F Z, Morningstar, M L, Wogan, G N
Reaction of synthetic N-(2'-deoxyguanosin-8-yl)-4-aminobiphenyl (dGuo-8-ABP) with t-butoxycarbonyl-L-[35S]methionine, N-hydroxysuccinimidyl ester (35S-labeled TBM-NHS), under optimized conditions...
Sequence alterations in the exon 1 region of the rat c-Ki-ras gene were studied in DNA isolated from aflatoxin B1 (AFB1)-induced rat liver carcinomas and precursor lesions appearing 56 weeks after...
Soman, N R, Correa, P, Ruiz, B A, Wogan, G N
The TPR-MET oncogenic rearrangement was originally observed in an in vitro transformed human osteosarcoma cell line. Recently, we detected the expression of this rearrangement at very low levels in...
Soman, N R, Wogan, G N, Rhim, J S
Activation of the MET protooncogene by a rearrangement involving the fusion of TPR and MET specific gene sequences has been observed in a human osteosarcoma cell line (HOS) treated in vitro with...
Mutations in c-Ki-ras oncogenes in diseased livers of winter flounder from Boston Harbor.
McMahon, G, Huber, L J, Moore, M J, Stegeman, J J, Wogan, G N
Livers of a natural population of winter flounder from a contaminated site in Boston Harbor were examined for the presence of oncogenes by transfection of DNA into NIH 3T3 mouse fibroblasts. Tissues...
Characterization of c-Ki-ras and N-ras oncogenes in aflatoxin B1-induced rat liver tumors.
McMahon, G, Davis, E F, Huber, L J, Kim, Y, Wogan, G N
c-Ki-ras and N-ras oncogenes have been characterized in aflatoxin B1-induced hepatocellular carcinomas. Detection of different protooncogene and oncogene sequences and estimation of their frequency...
Demain, A. L., Hunt, N. A., Malik, V., Kobbe, B., Hawkins, H., Matsuo, K., ...
This report describes an improved procedure for production of cytochalasin E and tremorgens by solid substrate, agitated fermentation of Aspergillus clavatus on pearled barley.
Production and antibacterial activity of malforming C, a toxic metabolite of Aspergillus niger.
Kobbe, B, Cushman, M, Wogan, G N, Demain, A L
The production of the new mycotoxin malformin C by a solid substrate fermentation is described. Malformin C is highly toxic (mean lethal dose = 0.9 mg/kg) and exerts antibacterial activity against a...
Mollicellins: mutagenic and antibacterial mycotoxins.
Stark, A A, Kobbe, B, Matsuo, D, Büchi, G, Wogan, G N, Demain, A L
Eight mollicellins (depsidones) were assayed for mutagenicity and antibacterial activity in Salmonella/microsome tests involving histidine reversion and forward mutation to 8-azaguanine resistance....
McMahon, G, Davis, E, Wogan, G N
Activated c-Ki-ras genes in liver tumors from rats exposed to the potent hepatocarcinogen aflatoxin B1 were analyzed to determine the nature of their activation by characterization of two c-Ki-ras...
Monoclonal antibody to aflatoxin B1-modified DNA detected by enzyme immunoassay.
Haugen, A, Groopman, J D, Hsu, I C, Goodrich, G R, Wogan, G N, Harris, C C
Monoclonal antibodies were obtained after fusion of mouse P3 X 63 myeloma cells with spleen cells isolated from BALB/c mice that had been immunized with aflatoxin B1-adducted DNA complexed with...
Quantitation of aflatoxin B1 adduction within the ribosomal RNA gene sequences of rat liver DNA.
The in vivo formation of covalent aflatoxin B1 (AFB1)-DNA adducts within the rRNA gene sequences of nuclear DNA has been studied in AFB1-treated rats. Liver nuclear DNA, enriched in ribosomal DNA...
Pezzuto, J M, Lau, P P, Luh, Y, Moore, P D, Wogan, G N, Hecht, S M
3-Amino-1-methyl-5H-pyrido[4,3-b]indole, previously reported to be a component of tryptophan pyrolysates, is an intensely mutagenic compound requiring microsomal activation for expression of...
In vitro reactions of aflatoxin B1-adducted DNA.
Groopman, J D, Croy, R G, Wogan, G N
The chemical stability of aflatoxin B1 bound to calf thymus DNA was studied over a 48-hour exposure to phosphate buffers at pH 6.8-8.0 (37 degrees C). During this time, aliquots of the aflatoxin...
Essigmann, J M, Barker, L J, Fowler, K W, Francisco, M A, Reinhold, V N, Wogan, G N
Sterigmatocystin (ST), a potent hepatocarcinogen, was covalently bound to calf thymus DNA by incubation in the presence of phenobarbital-induced rat liver microsomes. Acid hydrolysis of ST-modified...
Aflatoxin B1 mutagenesis, DNA binding, and adduct formation in Salmonella typhimurium.
Stark, A A, Essigmann, J M, Demain, A L, Skopek, T R, Wogan, G N
Salmonella typhimurium strain TM677 was mutagenized with aflatoxin B1 (AFB1) in liquid suspension culture in the presence of a rat liver postmitochondrial supernatant. Forward mutation to...
Identification of an activated c-Ki-ras oncogene in rat liver tumors induced by aflatoxin B1.
McMahon, G, Hanson, L, Lee, J J, Wogan, G N
Weanling male Fischer rats were administered 40 intraperitoneal injections of aflatoxin B1 (25 micrograms per animal per day) over a 2-month period. This chronic dosing regimen resulted in the...
Groopman, J D, Donahue, P R, Zhu, J Q, Chen, J S, Wogan, G N
A high-affinity IgM monoclonal antibody specific for aflatoxins was covalently bound to Sepharose 4B and used as a preparative column to isolate aflatoxin derivatives from the urine of people and...
Groopman, J D, Trudel, L J, Donahue, P R, Marshak-Rothstein, A, Wogan, G N
Monoclonal antibodies specific for aflatoxin B1, aflatoxin B2, aflatoxin M1, and the major aflatoxin-DNA adducts were obtained following fusion of mouse SP-2 myeloma cells with spleen cells of mice...
Identification of the principal aflatoxin B1-DNA adduct formed in vivo in rat liver.
Croy, R G, Essigmann, J M, Reinhold, V N, Wogan, G N
The products of in vivo covalent binding of activated aflatoxin B1 (AFB1) to DNA have been investigated in rats. The principal covalent product formed in liver DNA of rats treated with AFB1 has been...
Structural identification of the major DNA adduct formed by aflatoxin B1 in vitro.
Essigmann, J M, Croy, R G, Nadzan, A M, Busby, W F, Reinhold, V N, Büchi, G, ...
The covalent binding of the hepatocarcinogen aflatoxin B1 by rat liver microsomes to calf thymus DNA resulted in a binding level equal to one aflatoxin residue per 60 DNA nucleotides. An aflatoxin...
Molecular epidemiology is increasingly being applied in studies of cancer risks derived from exposure to environmental carcinogens of both endogenous and exogenous origins. Analytical methods have...
Groopman, J D, Wild, C P, Hasler, J, Junshi, C, Wogan, G N, Kensler, T W
Human epidemiology and experimental animal data have provided the statistical association and biological information necessary to propose that aflatoxins are risk factors for human liver cancer. As...
Markers of exposure to carcinogens.
Methods have been developed for the detection of exposure to carcinogens and other DNA damaging agents in experimental animals and man through the detection of carcinogens or their metabolic...
Chemical and biochemical dosimetry of exposure to genotoxic chemicals.
Epidemiologic studies designed to evaluate the health significance of environmental chemicals are compromised by the lack of quantitative exposure data for individuals in exposed populations....
Erdman, S. E., Rao, V. P., Poutahidis, T., Rogers, A. B., Taylor, C. L., Jackson, E. A., ...
Recombinase-activating gene-2-deficient (Rag2−/−) mice lacking functional lymphocytes provide a useful model of chronic inflammatory bowel disease-emulating events in human colon cancer....