Workshop on DNA repair. (1992)
Lehmann, A.R., Hoeijmakers, J.H.J., Zeeland, A.A. Van, Backendorf, C.M.P., Bridges, B.A., Collins, A., ...
A workshop on DNA repair with emphasis on eukaryotic systems was held, under the auspices of the EC Concerted Action on DNA Repair and Cancer, at Noordwijkerhout (The Netherlands) 14-19 April 1991....
Hang, B., Singer, B., Margison, G. P., Elder, R. H.
It has previously been reported that 1,N6-ethenoadenine (ɛA), deaminated adenine (hypoxanthine, Hx), and 7,8-dihydro-8-oxoguanine (8-oxoG), but not 3,N4-ethenocytosine (ɛC), are released from DNA...
Collis, S. J., Tighe, A., Scott, S. D., Roberts, S. A., Hendry, J. H., Margison, G. P.
The strand transferase RAD51 is a component of the homologous recombination repair pathway. To examine the contribution of RAD51 to the genotoxic effects of ionising radiation, we have used a novel...
Von Hofe, E, Fairbairn, L, Margison, G P
While a great deal of evidence has directly implicated the importance of O6-alkylation of guanine in the mutagenicity of alkylating agents, evidence demonstrating the oncogenic potential of this...
Collis, S. J., Sangar, V. K., Tighe, A., Roberts, S. A., Clarke, N. W., Hendry, J. H., ...
Cellular survival following ionising radiation-mediated damage is primarily a function of the ability to successfully detect and repair DNA double-strand breaks (DSBs). Previous studies have...
Kooistra, R, Zonneveld, J B, Watson, A J, Margison, G P, Lohman, P H, Pastink, A
The protein O 6-alkylguanine-DNA alkyltransferase(alkyltransferase) is involved in the repair of O 6-alkylguanine and O 4-alkylthymine in DNA and plays an important role in most organisms in...
Repair of O-alkylpyrimidines in mammalian cells: a present consensus.
Brent, T P, Dolan, M E, Fraenkel-Conrat, H, Hall, J, Karran, P, Laval, L, ...
Enzymatic repair of the O-alkylpyrimidines (O2- and O4-alkylthymine, O2-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase...
Potter, P M, Wilkinson, M C, Fitton, J, Carr, F J, Brennand, J, Cooper, D P, ...
The plasmid pO61 that was isolated from an E. coli genomic DNA library and codes for O6-alkylguanine (O6AG) DNA alkyltransferase (ATase) activity (1) has been further characterised. Subclones of the...
Brozmanová, J, Vlcková, V, Chovanec, M, Cernáková, L, Skorvaga, M, Margison, G P
The Escherichia coli ada gene protein coding region under the control of the yeast alcohol dehydrogenase promoter in the extrachromosomally replicating yeast expression vectors pADHO6C and pVT103LO6C...
Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.
Baker, S M, Margison, G P, Strike, P
We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes...
Rafferty, J A, Elder, R H, Watson, A J, Cawkwell, L, Potter, P M, Margison, G P
The cDNA encoding Chinese hamster O6-alkylguanine-DNA-alkyltransferase (ATase) has been isolated from a library prepared from RNA isolated from V79 lung fibroblasts which had an upregulated level of...
Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.
Rat and hamster liver extracts were found to contain DNA glycosylases capable of removing 3-methyladenine and 7-methylguanine from methylated DNA. The activity of 7-methylguanine-DNA glycosylase was...
Wilkinson, M C, Cooper, D P, Southan, C, Potter, P M, Margison, G P
O6-alkylguanine-DNA-alkyltransferase (ATase) activity was increased in rat liver from 80 to 320 fmoles/mg total protein 48 h after administration of 2-acetylaminofluorene at 60 mg/kg body weight....
Brozmanova, J, Kleibl, K, Vlckova, V, Skorvaga, M, Cernakova, L, Margison, G P
The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce...
Gonzaga, P E, Harris, L, Margison, G P, Brent, T P
Chloroethylnitrosoureas (CENUs) are thought to induce cytotoxic DNA interstrand cross-links via an initial reaction at O6-position of guanine, yielding a rearranged intermediate, O6,N1-ethanoguanine....
Expression of a human O6-alkylguanine-DNA-alkyltransferase cDNA in human cells and transgenic mice.
Fan, C Y, Potter, P M, Rafferty, J, Watson, A J, Cawkwell, L, Searle, P F, ...
A truncated human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA was ligated into an expression vector under the control of the mouse metallothionein-1 gene promotor and upstream of part of the...
Expression of the ogt gene in wild-type and ada mutants of E. coli.
Potter, P M, Kleibl, K, Cawkwell, L, Margison, G P
O6-alkylguanine (O6-AlkG) DNA alkyltransferase (ATase) and alkylphosphotriester (AlkP) ATase activity have been quantitated individually in extracts of various E. coli strains by means of ATase...
Wilkinson, M C, Potter, P M, Cawkwell, L, Georgiadis, P, Patel, D, Swann, P F, ...
The E. coli gene ogt encodes the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (O6-AlkG ATase). The protein coding region of the gene was cloned into a multicopy expression vector to obtain...
Margison, G P, Cooper, D P, Brennand, J
Alkylating agents react with various nitrogen and oxygen atoms in DNA and many of the products are substrates for repair processes. Oxygen atom derivatives such as O6-methylguanine (O6-meG)...
The toxic, mutagenic, and carcinogenic effects of alkylating agents have been attributed to their ability to damage DNA. Reaction at the O6 position of guanine results in miscoding during DNA...
Klungland, A, Fairbairn, L, Watson, A J, Margison, G P, Seeberg, E
In order to investigate the importance of 3-methyladenine in cellular sensitivity to chemical methylating agents we have constructed retroviral vectors for the integration and expression of the...
C-terminally truncated human O6-alkylguanine-DNA alkyltransferase retains activity.
Elder, R H, Tumelty, J, Douglas, K T, Margison, G P, Rafferty, J A
A cDNA encoding the human O6-alkylguanine-DNA alkyltransferase (ATase; EC 2.1.1.63; methylated-DNA: protein-cysteine methyltransferase) has been manipulated to generate a C-terminally deleted protein...
Elder, R H, Margison, G P, Rafferty, J A
The action of O6-benzylguanine (O6-BzlG) on recombinant mammalian and Escherichia coli O6-alkylguanine-DNA alkyltransferases (ATase; EC 2.1.1.63; methylated-DNA-protein-cysteine methyltransferase)...
Margison, G P, Margison, J M, Montesano, R
1. DNA was extracted from livers, kidneys and lungs of Syrian golden hamsters at various times (up to 96h) after injection of a hepatocarcinogenic dose of [14C]dimethylnitrosamine. Purine bases were...
Margison, G P, Margison, J M, Montesano, R
1. BD-IV rats were given labelled dimethylnitrosamine (2 mg/kg) by stomach tube on weekdays (Monday to Friday) for up to 24 weeks. The rats killed after 2, 4, 8, 16 and 24 weeks of treatment (72 h...
The alkylation of purine bases in DNA of several rat tissues was determined during weekly injections (10 mg/kg) of N-[3H]methyl-N-nitrosourea, a dose schedule known to selectively induce tumours of...
The effect of introducing methyl groups into DNA substrates was studied by using the spleen exonuclease (EC 3.1.4.1), an enzyme which hydrolyses oligonucleotides in a sequential manner by splitting...
Margison, G P, Margison, J M, Montesano, R
1. Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. The chemical alkyation products,...
Hang, B., Singer, B., Margison, G. P., Elder, R. H.
It has previously been reported that 1,N6-ethenoadenine (ɛA), deaminated adenine (hypoxanthine, Hx), and 7,8-dihydro-8-oxoguanine (8-oxoG), but not 3,N4-ethenocytosine (ɛC), are released from DNA...
Collis, S. J., Tighe, A., Scott, S. D., Roberts, S. A., Hendry, J. H., Margison, G. P.
The strand transferase RAD51 is a component of the homologous recombination repair pathway. To examine the contribution of RAD51 to the genotoxic effects of ionising radiation, we have used a novel...
Von Hofe, E, Fairbairn, L, Margison, G P
While a great deal of evidence has directly implicated the importance of O6-alkylation of guanine in the mutagenicity of alkylating agents, evidence demonstrating the oncogenic potential of this...
Collis, S. J., Sangar, V. K., Tighe, A., Roberts, S. A., Clarke, N. W., Hendry, J. H., ...
Cellular survival following ionising radiation-mediated damage is primarily a function of the ability to successfully detect and repair DNA double-strand breaks (DSBs). Previous studies have...
Kooistra, R, Zonneveld, J B, Watson, A J, Margison, G P, Lohman, P H, Pastink, A
The protein O 6-alkylguanine-DNA alkyltransferase(alkyltransferase) is involved in the repair of O 6-alkylguanine and O 4-alkylthymine in DNA and plays an important role in most organisms in...
Repair of O-alkylpyrimidines in mammalian cells: a present consensus.
Brent, T P, Dolan, M E, Fraenkel-Conrat, H, Hall, J, Karran, P, Laval, L, ...
Enzymatic repair of the O-alkylpyrimidines (O2- and O4-alkylthymine, O2-alkylcytosine) and alkyl phosphotriesters has been studied in Escherichia coli, and the two proteins involved, a glycosylase...
Potter, P M, Wilkinson, M C, Fitton, J, Carr, F J, Brennand, J, Cooper, D P, ...
The plasmid pO61 that was isolated from an E. coli genomic DNA library and codes for O6-alkylguanine (O6AG) DNA alkyltransferase (ATase) activity (1) has been further characterised. Subclones of the...
Brozmanová, J, Vlcková, V, Chovanec, M, Cernáková, L, Skorvaga, M, Margison, G P
The Escherichia coli ada gene protein coding region under the control of the yeast alcohol dehydrogenase promoter in the extrachromosomally replicating yeast expression vectors pADHO6C and pVT103LO6C...
Inducible alkyltransferase DNA repair proteins in the filamentous fungus Aspergillus nidulans.
Baker, S M, Margison, G P, Strike, P
We have investigated the response of the filamentous fungus Aspergillus nidulans to low, non-killing, doses of the alkylating agent MNNG (N-methyl-N'-nitro-N-nitrosoguanidine). Such treatment causes...
Rafferty, J A, Elder, R H, Watson, A J, Cawkwell, L, Potter, P M, Margison, G P
The cDNA encoding Chinese hamster O6-alkylguanine-DNA-alkyltransferase (ATase) has been isolated from a library prepared from RNA isolated from V79 lung fibroblasts which had an upregulated level of...
Enzymatic release of 7-methylguanine from methylated DNA by rodent liver extracts.
Rat and hamster liver extracts were found to contain DNA glycosylases capable of removing 3-methyladenine and 7-methylguanine from methylated DNA. The activity of 7-methylguanine-DNA glycosylase was...
Wilkinson, M C, Cooper, D P, Southan, C, Potter, P M, Margison, G P
O6-alkylguanine-DNA-alkyltransferase (ATase) activity was increased in rat liver from 80 to 320 fmoles/mg total protein 48 h after administration of 2-acetylaminofluorene at 60 mg/kg body weight....
Brozmanova, J, Kleibl, K, Vlckova, V, Skorvaga, M, Cernakova, L, Margison, G P
The E.coli ada gene protein coding region has been ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase gene promoter region to produce...
Gonzaga, P E, Harris, L, Margison, G P, Brent, T P
Chloroethylnitrosoureas (CENUs) are thought to induce cytotoxic DNA interstrand cross-links via an initial reaction at O6-position of guanine, yielding a rearranged intermediate, O6,N1-ethanoguanine....
Expression of a human O6-alkylguanine-DNA-alkyltransferase cDNA in human cells and transgenic mice.
Fan, C Y, Potter, P M, Rafferty, J, Watson, A J, Cawkwell, L, Searle, P F, ...
A truncated human O6-alkylguanine-DNA-alkyltransferase (ATase) cDNA was ligated into an expression vector under the control of the mouse metallothionein-1 gene promotor and upstream of part of the...
Expression of the ogt gene in wild-type and ada mutants of E. coli.
Potter, P M, Kleibl, K, Cawkwell, L, Margison, G P
O6-alkylguanine (O6-AlkG) DNA alkyltransferase (ATase) and alkylphosphotriester (AlkP) ATase activity have been quantitated individually in extracts of various E. coli strains by means of ATase...
Wilkinson, M C, Potter, P M, Cawkwell, L, Georgiadis, P, Patel, D, Swann, P F, ...
The E. coli gene ogt encodes the DNA repair protein O6-alkylguanine-DNA-alkyltransferase (O6-AlkG ATase). The protein coding region of the gene was cloned into a multicopy expression vector to obtain...
Margison, G P, Cooper, D P, Brennand, J
Alkylating agents react with various nitrogen and oxygen atoms in DNA and many of the products are substrates for repair processes. Oxygen atom derivatives such as O6-methylguanine (O6-meG)...
The toxic, mutagenic, and carcinogenic effects of alkylating agents have been attributed to their ability to damage DNA. Reaction at the O6 position of guanine results in miscoding during DNA...
Klungland, A, Fairbairn, L, Watson, A J, Margison, G P, Seeberg, E
In order to investigate the importance of 3-methyladenine in cellular sensitivity to chemical methylating agents we have constructed retroviral vectors for the integration and expression of the...
C-terminally truncated human O6-alkylguanine-DNA alkyltransferase retains activity.
Elder, R H, Tumelty, J, Douglas, K T, Margison, G P, Rafferty, J A
A cDNA encoding the human O6-alkylguanine-DNA alkyltransferase (ATase; EC 2.1.1.63; methylated-DNA: protein-cysteine methyltransferase) has been manipulated to generate a C-terminally deleted protein...
Elder, R H, Margison, G P, Rafferty, J A
The action of O6-benzylguanine (O6-BzlG) on recombinant mammalian and Escherichia coli O6-alkylguanine-DNA alkyltransferases (ATase; EC 2.1.1.63; methylated-DNA-protein-cysteine methyltransferase)...
Margison, G P, Margison, J M, Montesano, R
1. DNA was extracted from livers, kidneys and lungs of Syrian golden hamsters at various times (up to 96h) after injection of a hepatocarcinogenic dose of [14C]dimethylnitrosamine. Purine bases were...
Margison, G P, Margison, J M, Montesano, R
1. BD-IV rats were given labelled dimethylnitrosamine (2 mg/kg) by stomach tube on weekdays (Monday to Friday) for up to 24 weeks. The rats killed after 2, 4, 8, 16 and 24 weeks of treatment (72 h...
The alkylation of purine bases in DNA of several rat tissues was determined during weekly injections (10 mg/kg) of N-[3H]methyl-N-nitrosourea, a dose schedule known to selectively induce tumours of...
The effect of introducing methyl groups into DNA substrates was studied by using the spleen exonuclease (EC 3.1.4.1), an enzyme which hydrolyses oligonucleotides in a sequential manner by splitting...
Margison, G P, Margison, J M, Montesano, R
1. Syrian golden hamster liver ribosomal RNA was isolated up to 96 h after administration of [14C]dimethylnitrosamine at 25 mg/kg or 2.5 mg/kg body weight. 2. The chemical alkyation products,...