Efficacy of plant-produced recombinant antibodies against HCG (2002)
Kathuria, S., Sriraman, R., Nath, R., Sack, M., Pal, R., Artsaenko, O., ...
BACKGROUND: Antibody engineering facilitates the construction of different antibody formats [single chain variable fragment (scFv), diabody, full-size chimeric monoclonal antibody] with ease....
Functional recombinant antibodies against human chorionic gonadotropin expressed in plants (2002)
Kathuria, S.R., Nath, R., Pal, R., Singh, O., Fischer, R., Lohiya, N.K., ...
Single-chain variable fragments, diabodies and chimeric antibodies (mouse variable domains and human immunoglobulin constant domains) were engineered by DNA recombinant technique and expressed...
Mukhopadhyay, Asok, Mukhopadhyay, S N, Talwar, G P
Replication of vaccinia virus carrying β-hCG gene at the ‘tk’ locus was studied in microcarrier culture of Vero cell. The yields of recombinant vaccinia virus and its expressed product (β-hCG)...
A vaccine that prevents pregnancy in women.
Talwar, G P, Singh, O, Pal, R, Chatterjee, N, Sahai, P, Dhall, K, ...
We report here results of clinical trials on a birth control vaccine, consisting of a heterospecies dimer of the beta subunit of human chorionic gonadotropin (hCG) associated noncovalently with the...
Srinivasan, J, Singh, O, Chakrabarti, S, Talwar, G P
We carried out experiments designed to study the effect of a protein's localization on its immunogenicity. A novel cell-surface protein was generated from a small, glycosylated secretory protein. The...
In vitro characterization of T cells from Mycobacterium w-vaccinated mice.
Singh, I G, Mukherjee, R, Talwar, G P, Kaufmann, S H
Tuberculosis caused by the intracellular bacterial pathogen Mycobacterium tuberculosis still represents a major health problem, and its effective control would best be accomplished by active...
Qadri, A, Gupta, S K, Talwar, G P
Fifteen monoclonal antibodies (MAbs) directed against Salmonella typhi were produced and characterized. The specificities of the antibodies were determined by their binding patterns in an enzyme...
Talwar, G P, Sharma, N C, Dubey, S K, Salahuddin, M, Das, C, Ramakrishnan, S, ...
The immunogenicity of the conjugate prepared from "processed" beta-subunit of human chorionic gonadotropin (choriogonadotropin, HCG) and tetanus toxoid has been studied in animals and a human...
Talwar, G P, Gupta, S K, Singh, V, Sahal, D, Iyer, K S, Singh, O
A monoclonal antibody generated against the decapeptide gonadotropin-releasing hormone (GnRH) was effective in intercepting the bioactivity of the hormone; it blocked ovulation in rats. The antibody...
A practicable immunological approach to block spermatogenesis without loss of androgens.
Talwar, G P, Naz, R K, Das, C, Das, R P
The intrinsic capacity of the immune system to elicit immune response selectively against late developing sperm proteins has been mobilized to intercept spermatogenesis. Bacillus Calmette-Guérin...
Talwar, G. P., Krishnan, A. D., Gupta, P. D.
Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-3H]thymidine into...
Ramakrishnan, S, Das, C, Talwar, G P
The beta-subunit of human chorionic gonadotropin, purified immunochemically to eliminate undissociated human chorionic gonadotropin, induced testosterone production by mouse Leydig cells at...
Growth hormone stimulates the synthesis of RNA in hypophysectomized rat liver. The question whether the hormonal stimulation of RNA synthesis is due to the activation of repressed cistrons or to...
Nature and characteristics of the binding of oestradiol-17β to a uterine macromolecular fraction
Talwar, G. P., Sopori, M. L., Biswas, D. K., Segal, S. J.
The binding of oestradiol-17β to two proteins, namely serum albumin and a uterus fraction, was studied in vitro. The former protein has a physiological function in the transport of the hormone and...
A vaccine that prevents pregnancy in women.
Talwar, G P, Singh, O, Pal, R, Chatterjee, N, Sahai, P, Dhall, K, ...
We report here results of clinical trials on a birth control vaccine, consisting of a heterospecies dimer of the beta subunit of human chorionic gonadotropin (hCG) associated noncovalently with the...
Srinivasan, J, Singh, O, Chakrabarti, S, Talwar, G P
We carried out experiments designed to study the effect of a protein's localization on its immunogenicity. A novel cell-surface protein was generated from a small, glycosylated secretory protein. The...
In vitro characterization of T cells from Mycobacterium w-vaccinated mice.
Singh, I G, Mukherjee, R, Talwar, G P, Kaufmann, S H
Tuberculosis caused by the intracellular bacterial pathogen Mycobacterium tuberculosis still represents a major health problem, and its effective control would best be accomplished by active...
Qadri, A, Gupta, S K, Talwar, G P
Fifteen monoclonal antibodies (MAbs) directed against Salmonella typhi were produced and characterized. The specificities of the antibodies were determined by their binding patterns in an enzyme...
Talwar, G P, Sharma, N C, Dubey, S K, Salahuddin, M, Das, C, Ramakrishnan, S, ...
The immunogenicity of the conjugate prepared from "processed" beta-subunit of human chorionic gonadotropin (choriogonadotropin, HCG) and tetanus toxoid has been studied in animals and a human...
Talwar, G P, Gupta, S K, Singh, V, Sahal, D, Iyer, K S, Singh, O
A monoclonal antibody generated against the decapeptide gonadotropin-releasing hormone (GnRH) was effective in intercepting the bioactivity of the hormone; it blocked ovulation in rats. The antibody...
A practicable immunological approach to block spermatogenesis without loss of androgens.
Talwar, G P, Naz, R K, Das, C, Das, R P
The intrinsic capacity of the immune system to elicit immune response selectively against late developing sperm proteins has been mobilized to intercept spermatogenesis. Bacillus Calmette-Guérin...
Talwar, G. P., Krishnan, A. D., Gupta, P. D.
Growth of intracellular parasites such as Mycobacterium leprae in macrophages derived from human peripheral blood monocytes can be assessed by selective incorporation of [methyl-3H]thymidine into...
Ramakrishnan, S, Das, C, Talwar, G P
The beta-subunit of human chorionic gonadotropin, purified immunochemically to eliminate undissociated human chorionic gonadotropin, induced testosterone production by mouse Leydig cells at...
Growth hormone stimulates the synthesis of RNA in hypophysectomized rat liver. The question whether the hormonal stimulation of RNA synthesis is due to the activation of repressed cistrons or to...
Nature and characteristics of the binding of oestradiol-17β to a uterine macromolecular fraction
Talwar, G. P., Sopori, M. L., Biswas, D. K., Segal, S. J.
The binding of oestradiol-17β to two proteins, namely serum albumin and a uterus fraction, was studied in vitro. The former protein has a physiological function in the transport of the hormone and...
Carrier-induced suppression of the antibody response to a 'self' hapten.
Sad, S, Gupta, H M, Talwar, G P, Raghupathy, R
Immunization of male rats and monkeys with gonadotropin-releasing hormone (GnRH) conjugated to a carrier results in a dramatic atrophy of the prostate. GnRH, linked to either diphtheria toxoid or...
Vaccine for control of fertility
Talwar, G. P., Singh, O., Jayashankar, R., Shaha, C., Suri, A., Rao, L. V., ...
Birth control vaccines constitute a new category of vaccines. Immunization with the objective of selectively blocking a physiological process differs in many respects from immunizing against...
Bypass of carrier-induced epitope-specific suppression using a T-helper epitope.
Sad, S, Rao, K, Arora, R, Talwar, G P, Raghupathy, R
A gonadotropin-releasing hormone (GnRH)-based vaccine is being developed as a method for non-surgical immunotherapy as immunization with this vaccine results in atrophy of the prostate. This vaccine,...
Depression of T-cell function and normality of B-cell response in protein calorie malnutrition.
Narayanan, R B, Nath, I, Bhuyan, U N, Talwar, G P
Antibody response to a T-cell dependent antigen, sheep erythrocytes and to a B-cell mitogen, purified lipopolysaccharide (LPS), has been studied in mice kept on protein deficient (2 and 4 per cent...
Effect of vitamin A and protein-calorie undernutrition on immune responses
Krishnan, Savithry, Bhuyan, U. N., Talwar, G. P., Ramalingaswami, V.
In rats rendered vitamin A-deficient, there was a marked atrophy of the thymus and the spleen. To a large extent, but not completely, these changes were a result of the concomitant protein-calorie...
Moudgil, K D, Gupta, S K, Naraynan, P R, Srivastava, L M, Mishra, R S, Talwar, G P
Twenty-six inbred BALB/cBy mice were infected with live Mycobacterium leprae by injecting 6 x 10(3) bacilli in the hind footpad. Bleeds were collected at monthly intervals. After 6 months, acid-fast...
Fotedar, A, Mustafa, A S, Narang, B S, Talwar, G P
Two acetoacetylated derivatives of Mycobacterium leprae with variable hapten groups and a conjugate with tetanus toxoid were prepared. These were tested as antigens along with unmodified M. leprae in...
Reduction of a subpopulation of T lymphocytes in lepromatous leprosy
Nath, Indira, Curtis, Jill, Bhutani, L. K., Talwar, G. P.
A reduction in number of a subpopulation of T lymphocytes was noted in lepromatous leprosy cases with high bacillary load. Tuberculoid and treated lepromatous patients, who were bacillary negative...
Nath, I, Curtis, J, Sharma, A K, Talwar, G P
The effect of treatment and mycobacterial load on circulating T-cell numbers and their functional ability was investigated in forty-one patients with leprosy. Both early binding T cells and their...
Evaluation of cell mediated immune responses in untreated cases of leprosy
Talwar, G. P., Krishnan, A. D., Mehra, V. L., Blum, E. A., Pearson, J. M. H.
Twenty-three leprosy patients have been studied in an endemic area before institution of chemotherapy. These were comprised of ten lepromatous leprosy, four borderline lepromatous and nine...
Mehra, V. L., Talwar, G. P., Balakrishnan, K., Bhutani, L. K.
Mitogenic response to phytohaemagglutinin (PHA) of peripheral leucocytes from patients with polar forms of leprosy namely lepromatous leprosy (LL) and tuberculoid leprosy (TT) has been evaluated. No...
The effect of pituitary growth hormone on the biosynthesis of DNA in the thymus and other lymphoid organs, as well as the ability of the rat to respond immunologically to sheep red blood cells, has...