Gustafson, JP, Taylor, J., Stacey, G.
The title will develop from the 24th Stadler symposium. It will explore the general theme 'GENOME EXPLOITATION: Data Mining the Genomes'. The idea behind the theme is to discuss and illustrate how...
Far-Infrared Spectroscopy Of Normal Galaxies With Iso-Lws (2007)
S. Malhotra, Malhotra Helou, D. Hollenbach, M. Kaufman, S. Lord, J. Brauher, ...
The deficiency of [CII] (158 ¯m) line emission in many normal and ultraluminous galaxies is one of the major surprises from ISO-LWS observations. We show that this is not an isolated phenomenon:...
Chandra and Spitzer unveil heavily obscured quasars in the SWIRE/Chandra Survey (2006)
Polletta, M., Wilkes, B. J., Siana, B., Lonsdale, C. J., Kilgard, R., Smith, H. E., ...
Using the large multi-wavelength data set in the chandra/SWIRE Survey (0.6 square degrees in the Lockman Hole), we show evidence for the existence of highly obscured (Compton-thick) AGN, estimate a...
Jarrett, T. H., Polletta, M., Fournon, I. P., Stacey, G., Xu, K., Siana, B., ...
We present ground-based optical and Spitzer infrared imaging observations of the interacting galaxy UGC 10214, the "Tadpole Galaxy" (z = 0.0310), focusing on the star formation activity in the...
Morphological Studies of the SWIRE Galaxy Population in the UGC 10214 HST/ACS field (2005)
Hatziminaoglou, E., Cassata, P., Rodighiero, G., Perez-Fournon, I., Franceschini, A., Hernan-Caballero, A., ...
We present results of a morphological analysis of a small subset of the Spitzer Wide-area InfraRed Extragalactic survey (SWIRE) galaxy population. The analysis is based on public ACS data taken...
Rowan-Robinson, M., Babbedge, T., Surace, J., Shupe, D., Fang, F., Lonsdale, C., ...
We discuss optical associations, spectral energy distributions and photometric redshifts for SWIRE sources in the ELAIS-N1 area and the Lockman Validation Field. The band-merged IRAC (3.6, 4.5, 5.8...
FIR-bright Galaxies with an Extreme [CII] Deficiency: Where are the PDRs? (2004)
Malhotra, S., Beichman, C. A., Dinerstein, H. L., Helou, G., Hollenbach, D. J., Hunter, D. A., ...
Fir Spectroscopy of the Nucleus and IR-Ring of the AGN/Starburst NGC 4945 (2004)
Lord, S., Unger, S., Brauher, J., Clegg, P., Cox, P., Fischer, J., ...
Physical Conditions in the ISM in Elliptical Galaxies (2004)
Malhotra, S., Valjavec, E., Helou, G., Hunter, D., Hollenbach, D., Lord, S., ...
The [CII] and [OI] Emission Lines in NGC1313 and NGC6946 (2004)
Contursi, A., Helou, G., Brauher, J., Dale, D., Dinerstein, H., Hollenbach, D., ...
ISOCAM Observations of Normal Star-Forming Galaxies: The Key Project Sample (2004)
Dale, D., Silbermann, N., Helou, G., Valjavec, E., Malhotra, S., Beichman, C., ...
We present mid-infrared maps and preliminary analysis for 61 galaxies observed with the Infrared Space Observatory.
Observations of gas and dust at the Galactic Centre (2003)
White, Glenn J., Smith, H. A., Stacey, G., Bradford, C. Matt, Leeks, S. J., Sweet, O., ...
The Infrared Space Observatory Long wavelength Spectrometerhas been used to map distribution of the emission from a sample of 22 atomic, molecular and ionised lines toward the Circumnuclear Disk at...
Far Infrared Spectroscopy of Normal Galaxies: Physical Conditions in the Interstellar Medium (2001)
Malhotra, S., Kaufman, M. J., Hollenbach, D., Helou, G., Rubin, R. H., Brauher, J., ...
The most important cooling lines of the neutral interstellar medium (ISM) lie in the far-infrared (FIR). We present measurements by the Infrared Space Observatory Long Wavelength Spectrometer of...
ISO LWS Spectroscopy of M82: A Unified Evolutionary Model (1998)
Colbert, J. W., Malkan, M. A., Clegg, P. E., Cox, P., Fischer, J., Lord, S. D., ...
We present the first complete far-infrared spectrum (43 to 197 um) of M82, the brightest infrared galaxy in the sky, taken with the Long Wavelength Spectrometer of the Infrared Space Observatory...
ISO measurements of [CII] line variations in galaxies (1997)
Malhotra, S., Helou, G., Stacey, G., Hollenbach, D., Lord, S., Beichman, C. A., ...
We report measurements of the [CII] fine structure line at 157.714 micron in 30 normal star-forming galaxies with the Long Wavelength Spectrometer (LWS) on the Infrared Space Observatory (ISO). The...
ISOCAM Observations of NGC 6946: Mid-IR Structure (1996)
Malhotra, S., Helou, G., Van Buren, D., Kong, M., Beichman, C. A., Dinerstein, H., ...
The nearby spiral galaxy NGC 6946 was observed with ISO-CAM in the mid-infrared, achieving 7 arcsec resolution and sub-MJy/steradian sensitivity. Images taken with CAM filters LW2 (7 microns) and LW3...
The Mid-Infrared Color of NGC 6946 (1996)
Helou, G., Malhotra, S., Beichman, C. A., Dinerstein, H., Hollenbach, D. J., Hunter, D. A., ...
We analyze the new mid-infrared maps of NGC 6946 for variations in the color ratio of the 7-to-15 micron emission. Our preliminary findings are that this mid-infrared color is remarkably constant...
Risk assessment of animal cell culture procedures. (1995)
The generation of continuous cell lines from animal and human tissues has yielded valuable tools for biological studies. Some of these cell lines have also proved important hosts for expression of...
Risk assessment of animal cell culture procedures. (1995)
The generation of continuous cell lines from animal and human tissues has yielded valuable tools for biological studies. Some of these cell lines have also proved important hosts for expression of...
Risk assessment of animal cell culture procedures. (1995)
The generation of continuous cell lines from animal and human tissues has yielded valuable tools for biological studies. Some of these cell lines have also proved important hosts for expression of...
Roberts, D M, Besl, L, Oh, S H, Masterson, R V, Schell, J, Stacey, G
Transgenic plants were constructed that express two foreign calmodulins (VU-1 and VU-3 calmodulins) derived from a cloned synthetic calmodulin gene. VU-1 calmodulin, similar to endogenous plant...
Sanjuan, J, Carlson, R W, Spaink, H P, Bhat, U R, Barbour, W M, Glushka, J, ...
Bradyrhizobium japonicum is a soil bacterium that forms nitrogen-fixing nodules on the roots of the agronomically important legume soybean. Microscopic observation of plant roots showed that butanol...
Sadowsky, M J, Cregan, P B, Gottfert, M, Sharma, A, Gerhold, D, Rodriguez-Quinones, F, ...
Several soybean genotypes have been identified which specifically exclude nodulation by members of Bradyrhizobium japonicum serocluster 123. We have identified and sequenced a DNA region from B....
Stokkermans, T J, Ikeshita, S, Cohn, J, Carlson, R W, Stacey, G, Ogawa, T, ...
Rhizobia synthesize a class of lipo-chitin oligosaccharides that induce root hair deformation and induce the initiation of nodule structures on legume roots. These lipo-chitin oligosaccharides are...
Cloning of a second Arabidopsis peptide transport gene.
Song, W, Steiner, H Y, Zhang, L, Naider, F, Stacey, G, Becker, J M
Previously, we reported the isolation of a peptide transport gene designated AtPTR2 from Arabidopsis thaliana by functional complementation of a yeast peptide transport mutant. We now report the...
Song, W, Koh, S, Czako, M, Marton, L, Drenkard, E, Becker, J M, ...
Previously, we identified a peptide transport gene, AtPTR2-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant...
An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins.
Steiner, H Y, Song, W, Zhang, L, Naider, F, Becker, J M, Stacey, G
An Arabidopsis peptide transport gene was cloned from an Arabidopsis cDNA library by functionally complementing a yeast peptide transport mutant. The Arabidopsis plant peptide transporter (AtPTR2)...
In Bradyrhizobium japonicum, members of two global regulatory families, a LysR-type regulator, NodD1, and a two-component regulatory system, NodVW, positively regulate nod gene expression in response...
Chemical control of interstrain competition for soybean nodulation by Bradyrhizobium japonicum.
Cunningham, S, Kollmeyer, W D, Stacey, G
Previous research has shown that a significant limitation to the agricultural use of improved rhizobial inoculant strains is competition from the indigenous soil population. In this work, we sought...
Chemotaxis of Bradyrhizobium japonicum to soybean exudates.
Barbour, W M, Hattermann, D R, Stacey, G
The chemotactic response of Bradyrhizobium japonicum toward soybean seed and root exudates was examined. Assays using various isoflavones and fractionated exudate indicated that isoflavones are not...
Efficient DNA transformation of Bradyrhizobium japonicum by electroporation.
Intact cells of Bradyrhizobium japonicum USDA 110 were transformed with a 30-kilobase plasmid to efficiencies of 10(6) to 10(7) transformants per microgram by high-voltage electroporation. The...
Chun, J Y, Sexton, G L, Roth, L E, Stacey, G
To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic...
Localizations of aluminum in soybean bacteroids and seeds.
Roth, L E, Dunlap, J R, Stacey, G
Aluminum, long known to be detrimental to soybean productivity, was localized in the polyphosphate granules (PPG) of bacteroids in root nodules of soybean plants. By using energy-dispersive X-ray...
Diversity of retron elements in a population of rhizobia and other gram-negative bacteria.
Rice, S A, Bieber, J, Chun, J Y, Stacey, G, Lampson, B C
Genetic elements called retrons reside on the chromosome of Escherichia coli and the myxobacteria and represent the first reverse transcriptase-encoding element to be found in a prokaryotic cell. All...
Stacey, G, Luka, S, Sanjuan, J, Banfalvi, Z, Nieuwkoop, A J, Chun, J Y, ...
The nodulation genes of rhizobia are regulated by the nodD gene product in response to host-produced flavonoids and appear to encode enzymes involved in the production of a lipo-chitose signal...
Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box.
Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the...
Identification of Bradyrhizobium nod genes involved in host-specific nodulation.
Three loci important for soybean nodulation by Bradyrhizobium japonicum were delimited by Tn5 mutagenesis on a 5.3-kilobase EcoRI fragment adjacent to the nodABC genes. Results of hybridization...
Immunogold localization of the NodC and NodA proteins of Rhizobium meliloti.
Johnson, D, Roth, L E, Stacey, G
Monospecific, polyclonal antibodies to the nodC and nodA gene products of Rhizobium meliloti were used in combination with immunogold labeling and transmission electron microscopy to localize the...
Cell surface polysaccharides from Bradyrhizobium japonicum and a nonnodulating mutant.
Puvanesarajah, V, Schell, F M, Gerhold, D, Stacey, G
The cell surface polysaccharides of wild-type Bradyrhizobium japonicum USDA 110 and a nonnodulating mutant, strain HS123, were analyzed. The capsular polysaccharide (CPS) and exopolysaccharide (EPS)...
Cangelosi, G A, Hung, L, Puvanesarajah, V, Stacey, G, Ozga, D A, Leigh, J A, ...
Mutants of Rhizobium meliloti have been isolated which are deficient in exopolysaccharide (EPS) production and effective nodulation of alfalfa (J. A. Leigh, E. R. Signer, and G. C. Walker, Proc....
A locus encoding host range is linked to the common nodulation genes of Bradyrhizobium japonicum.
Nieuwkoop, A J, Banfalvi, Z, Deshmane, N, Gerhold, D, Schell, M G, Sirotkin, K M, ...
By using cloned Rhizobium meliloti, Rhizobium leguminosarum, and Rhizobium sp. strain MPIK3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing...
Role for 2-linked-beta-D-glucan in the virulence of Agrobacterium tumefaciens.
Puvanesarajah, V, Schell, F M, Stacey, G, Douglas, C J, Nester, E W
Phenol-water cell extracts of virulent Agrobacterium tumefaciens A348 and several avirulent mutants with a reduced ability to attach to plant surfaces were examined. A low-molecular-weight...
Control of heterocyst and nitrogenase synthesis in cyanobacteria.
Stacey, G, Bottomley, P J, Van Baalen, C, Tabita, F R
The development of the heterocyst by filamentous nitrogen-fixing cyanobacteria provides an attractive model system for studying cellular differentiation. Heterocyst synthesis is repressed by the...
Russell, P, Schell, M G, Nelson, K K, Halverson, L J, Sirotkin, K M, Stacey, G
The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a...
Intragenic complementation by the nifJ-coded protein of Klebsiella pneumoniae.
Stacey, G, Zhu, J, Shah, V K, Shen, S C, Brill, W J
A single mutation, nifC1005 (Jin et al. Sci. Sin. 23:108-118, 1980), located between nifH and nifJ in the nif cluster of Klebsiella pneumoniae, genetically complemented mutations in each of the 17...
Rhizobium japonicum mutants defective in symbiotic nitrogen fixation.
Noel, K D, Stacey, G, Tandon, S R, Silver, L E, Brill, W J
Rhizobium japonicum strains 3I1b110 and 61A76 were mutagenized to obtain 25 independently derived mutants that produced soybean nodules defective in nitrogen fixation, as assayed by acetylene...
The nodulation characteristics of wild-type Bradyrhizobium japonicum USDA 110 and mutant strain HS111 were examined. Mutant strain HS111 exhibits a delayed-nodulation phenotype, a result of its...
Stacey, G, Pocratsky, L A, Puvanesarajah, V
A bacteriophage (phage TN1) that lyses Rhizobium japonicum 3I1b110 was isolated from Tennessee soil. Structurally, this phage resembles the Escherichia coli phage T4, having an icosahedral head (47...
Roberts, D M, Besl, L, Oh, S H, Masterson, R V, Schell, J, Stacey, G
Transgenic plants were constructed that express two foreign calmodulins (VU-1 and VU-3 calmodulins) derived from a cloned synthetic calmodulin gene. VU-1 calmodulin, similar to endogenous plant...
Sanjuan, J, Carlson, R W, Spaink, H P, Bhat, U R, Barbour, W M, Glushka, J, ...
Bradyrhizobium japonicum is a soil bacterium that forms nitrogen-fixing nodules on the roots of the agronomically important legume soybean. Microscopic observation of plant roots showed that butanol...
Sadowsky, M J, Cregan, P B, Gottfert, M, Sharma, A, Gerhold, D, Rodriguez-Quinones, F, ...
Several soybean genotypes have been identified which specifically exclude nodulation by members of Bradyrhizobium japonicum serocluster 123. We have identified and sequenced a DNA region from B....
Stokkermans, T J, Ikeshita, S, Cohn, J, Carlson, R W, Stacey, G, Ogawa, T, ...
Rhizobia synthesize a class of lipo-chitin oligosaccharides that induce root hair deformation and induce the initiation of nodule structures on legume roots. These lipo-chitin oligosaccharides are...
Cloning of a second Arabidopsis peptide transport gene.
Song, W, Steiner, H Y, Zhang, L, Naider, F, Stacey, G, Becker, J M
Previously, we reported the isolation of a peptide transport gene designated AtPTR2 from Arabidopsis thaliana by functional complementation of a yeast peptide transport mutant. We now report the...
Song, W, Koh, S, Czako, M, Marton, L, Drenkard, E, Becker, J M, ...
Previously, we identified a peptide transport gene, AtPTR2-B, from Arabidopsis thaliana that was constitutively expressed in all plant organs, suggesting an important physiological role in plant...
An Arabidopsis peptide transporter is a member of a new class of membrane transport proteins.
Steiner, H Y, Song, W, Zhang, L, Naider, F, Becker, J M, Stacey, G
An Arabidopsis peptide transport gene was cloned from an Arabidopsis cDNA library by functionally complementing a yeast peptide transport mutant. The Arabidopsis plant peptide transporter (AtPTR2)...
In Bradyrhizobium japonicum, members of two global regulatory families, a LysR-type regulator, NodD1, and a two-component regulatory system, NodVW, positively regulate nod gene expression in response...
Chemical control of interstrain competition for soybean nodulation by Bradyrhizobium japonicum.
Cunningham, S, Kollmeyer, W D, Stacey, G
Previous research has shown that a significant limitation to the agricultural use of improved rhizobial inoculant strains is competition from the indigenous soil population. In this work, we sought...
Chemotaxis of Bradyrhizobium japonicum to soybean exudates.
Barbour, W M, Hattermann, D R, Stacey, G
The chemotactic response of Bradyrhizobium japonicum toward soybean seed and root exudates was examined. Assays using various isoflavones and fractionated exudate indicated that isoflavones are not...
Efficient DNA transformation of Bradyrhizobium japonicum by electroporation.
Intact cells of Bradyrhizobium japonicum USDA 110 were transformed with a 30-kilobase plasmid to efficiencies of 10(6) to 10(7) transformants per microgram by high-voltage electroporation. The...
Chun, J Y, Sexton, G L, Roth, L E, Stacey, G
To understand the genetic mechanism of host specificity in the interaction between rhizobia and their hosts, it is important to identify genes that influence both early and late steps in symbiotic...
Localizations of aluminum in soybean bacteroids and seeds.
Roth, L E, Dunlap, J R, Stacey, G
Aluminum, long known to be detrimental to soybean productivity, was localized in the polyphosphate granules (PPG) of bacteroids in root nodules of soybean plants. By using energy-dispersive X-ray...
Diversity of retron elements in a population of rhizobia and other gram-negative bacteria.
Rice, S A, Bieber, J, Chun, J Y, Stacey, G, Lampson, B C
Genetic elements called retrons reside on the chromosome of Escherichia coli and the myxobacteria and represent the first reverse transcriptase-encoding element to be found in a prokaryotic cell. All...
Stacey, G, Luka, S, Sanjuan, J, Banfalvi, Z, Nieuwkoop, A J, Chun, J Y, ...
The nodulation genes of rhizobia are regulated by the nodD gene product in response to host-produced flavonoids and appear to encode enzymes involved in the production of a lipo-chitose signal...
Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box.
Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the...
Identification of Bradyrhizobium nod genes involved in host-specific nodulation.
Three loci important for soybean nodulation by Bradyrhizobium japonicum were delimited by Tn5 mutagenesis on a 5.3-kilobase EcoRI fragment adjacent to the nodABC genes. Results of hybridization...
Immunogold localization of the NodC and NodA proteins of Rhizobium meliloti.
Johnson, D, Roth, L E, Stacey, G
Monospecific, polyclonal antibodies to the nodC and nodA gene products of Rhizobium meliloti were used in combination with immunogold labeling and transmission electron microscopy to localize the...
Cell surface polysaccharides from Bradyrhizobium japonicum and a nonnodulating mutant.
Puvanesarajah, V, Schell, F M, Gerhold, D, Stacey, G
The cell surface polysaccharides of wild-type Bradyrhizobium japonicum USDA 110 and a nonnodulating mutant, strain HS123, were analyzed. The capsular polysaccharide (CPS) and exopolysaccharide (EPS)...
Cangelosi, G A, Hung, L, Puvanesarajah, V, Stacey, G, Ozga, D A, Leigh, J A, ...
Mutants of Rhizobium meliloti have been isolated which are deficient in exopolysaccharide (EPS) production and effective nodulation of alfalfa (J. A. Leigh, E. R. Signer, and G. C. Walker, Proc....
A locus encoding host range is linked to the common nodulation genes of Bradyrhizobium japonicum.
Nieuwkoop, A J, Banfalvi, Z, Deshmane, N, Gerhold, D, Schell, M G, Sirotkin, K M, ...
By using cloned Rhizobium meliloti, Rhizobium leguminosarum, and Rhizobium sp. strain MPIK3030 nodulation (nod) genes as hybridization probes, homologous regions were detected in the slow-growing...
Role for 2-linked-beta-D-glucan in the virulence of Agrobacterium tumefaciens.
Puvanesarajah, V, Schell, F M, Stacey, G, Douglas, C J, Nester, E W
Phenol-water cell extracts of virulent Agrobacterium tumefaciens A348 and several avirulent mutants with a reduced ability to attach to plant surfaces were examined. A low-molecular-weight...
Control of heterocyst and nitrogenase synthesis in cyanobacteria.
Stacey, G, Bottomley, P J, Van Baalen, C, Tabita, F R
The development of the heterocyst by filamentous nitrogen-fixing cyanobacteria provides an attractive model system for studying cellular differentiation. Heterocyst synthesis is repressed by the...
Russell, P, Schell, M G, Nelson, K K, Halverson, L J, Sirotkin, K M, Stacey, G
The DNA region encoding early nodulation functions of Bradyrhizobium japonicum 3I1b110 (I110) was isolated by its homology to the functionally similar region from Rhizobium meliloti. Isolation of a...
Intragenic complementation by the nifJ-coded protein of Klebsiella pneumoniae.
Stacey, G, Zhu, J, Shah, V K, Shen, S C, Brill, W J
A single mutation, nifC1005 (Jin et al. Sci. Sin. 23:108-118, 1980), located between nifH and nifJ in the nif cluster of Klebsiella pneumoniae, genetically complemented mutations in each of the 17...
Rhizobium japonicum mutants defective in symbiotic nitrogen fixation.
Noel, K D, Stacey, G, Tandon, S R, Silver, L E, Brill, W J
Rhizobium japonicum strains 3I1b110 and 61A76 were mutagenized to obtain 25 independently derived mutants that produced soybean nodules defective in nitrogen fixation, as assayed by acetylene...
The nodulation characteristics of wild-type Bradyrhizobium japonicum USDA 110 and mutant strain HS111 were examined. Mutant strain HS111 exhibits a delayed-nodulation phenotype, a result of its...
Stacey, G, Pocratsky, L A, Puvanesarajah, V
A bacteriophage (phage TN1) that lyses Rhizobium japonicum 3I1b110 was isolated from Tennessee soil. Structurally, this phage resembles the Escherichia coli phage T4, having an icosahedral head (47...