Carpenter, L., Mitchell, C. J., Xu, Z. Z., Poronnik, P., Both, G. W., Biden, T. J.
The role of protein kinase C (PKC) in glucose-stimulated insulin secretion (GSIS) is controversial. Using recombinant adenoviruses for overexpression of PKCalpha and PKCdelta, in both wild-type (WT)...
Carpenter, L., Mitchell, C. J., Xu, Z. Z., Poronnik, P., Both, G. W., Biden, T. J.
The role of protein kinase C (PKC) in glucose-stimulated insulin secretion (GSIS) is controversial. Using recombinant adenoviruses for overexpression of PKCalpha and PKCdelta, in both wild-type (WT)...
Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus (1984)
Both, G.W., Bellamy, A.R., Siegman, L.J.
A full length cDNA copy of dsRNA segment seven of Simian 11 rotavirus has been obtained by standard molecular cloning techniques. Segment seven codes for the non-structural viral protein NCVP4 and is...
Both, G. W., Bellamy, A. R., Street, J. E., Siegman, L. J.
Using Simian-11 rotavirus RNA, a strategy has been developed for the production of full length cloned copies of the genes of double-stranded (dsRNA) viruses. Genomic RNA segments were polyadenylated...
The complete nucleotide sequence has been determined for a cloned double-stranded DNA copy of the haenagglutinin gene from the human influenza strain A/NT/60/68/29C, a laboratory-isolated variant of...
A new method for the size estimation of the RNA genome segments of influenza virus (1979)
Sleigh, M.J., Both, G.W., Brownlee, G.G.
Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem...
Sleigh, M.J., Both, G.W., Brownlee, G.G.
A protocol has been developed for the synthesis of a double-stranded DNA (dsDNA) copy of the influenza virus RNA genome segment which codes for the major surface antigen, haemagglutinin (HA). This...
Sleigh, M J, Both, G W, Underwood, P A, Bender, V J
The nucleotide sequence of the gene coding for the large subunit of influenza virus hemagglutinin (HA1) was determined for strains A/NT/60/68, A/Eng/878/69, and A/Qu/7/70, three early isolates of the...
The nucleotide sequence was determined for the hemagglutinin gene of the Hong Kong subtype influenza strain A/Bangkok/1/79. The amino acid sequence predicted from these data shows a total of 36 amino...
Clarke, M L, Lockett, L J, Both, G W
The sequences responsible for binding rotavirus glycoprotein VP7 to the membrane of the endoplasmic reticulum (ER) have not been identified. Here we show that the sequences which promote membrane...
Andrew, M E, Boyle, D B, Whitfeld, P L, Lockett, L J, Anthony, I D, Bellamy, A R, ...
The glycoprotein VP7, the major serotype antigen of rotaviruses, is localized to the endoplasmic reticulum (ER) of the cell, where it is retained as a membrane-associated protein before assembly into...
Andrew, M E, Boyle, D B, Coupar, B E, Whitfeld, P L, Both, G W, Bellamy, A R
A cDNA copy of the gene coding for the major outer neutralizing protein (VP7) of simian 11 rotavirus was incorporated into the vaccinia virus genome under the control of the vaccinia promoter...
Both, G W, Siegman, L J, Bellamy, A R, Ikegami, N, Shatkin, A J, Furuichi, Y
Cloned DNA copies of rotavirus genomic segment 6 from simian 11 (subgroup 1) and human strain Wa (subgroup 2) rotaviruses have been used to determine the nucleotide sequences of the gene that...
Gunn, P R, Sato, F, Powell, K F, Bellamy, A R, Napier, J R, Harding, D R, ...
The rotavirus neutralizing antigen, VP7, is a 37,000-molecular-weight glycoprotein which is a major component of the outer shell of the virion. The amino acid sequence of VP7 for strain S2 (human...
Both, G W, Sleigh, M J, Cox, N J, Kendal, A P
Surveys of the antigenic properties of a wide range of variants of the H3N2 (Hong Kong) influenza virus subtype have revealed complex patterns of variants cocirculating during each of the main...
Both, G W, Siegman, L J, Bellamy, A R, Atkinson, P H
A cloned DNA copy of simian rotavirus SA11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein NCVP5 to this gene. Determination of the nucleotide sequence for...
Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus.
Both, G W, Bellamy, A R, Siegman, L J
A full length cDNA copy of dsRNA segment seven of Simian 11 rotavirus has been obtained by standard molecular cloning techniques. Segment seven codes for the non-structural viral protein NCVP4 and is...
The complete nucleotide sequence has been determined for a cloned double-stranded DNA copy of the haemagglutinin gene from the human influenza strain A/NT/60/68/29C, a laboratory-isolated variant of...
Both, G W, Bellamy, A R, Street, J E, Siegman, L J
Using Simian-11 rotavirus RNA, a strategy has been developed for the production of full length cloned copies of the genes of double-stranded (dsRNA) viruses. Genomic RNA segments were polyadenylated...
A new method for the size estimation of the RNA genome segments of influenza virus.
Sleigh, M J, Both, G W, Brownlee, G G
Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem...
Influence of the sequence-dependent flexure of DNA on transcription in E. coli.
Collis, C M, Molloy, P L, Both, G W, Drew, H R
In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or...
The influenza virus haemagglutinin gene: cloning and characterisation of a double-stranded DNA copy.
Sleigh, M J, Both, G W, Brownlee, G G
A protocol has been developed for the synthesis of a double-stranded DNA (dsDNA) copy of the influenza virus RNA genome segment which codes for the major surface antigen, haemagglutinin (HA). This...
Both, G W, Moyer, S A, Banerjee, A K
The cytoplasm of vesicular stomatitis virus (VSV)-infected BHK cells has been separated into a fraction containing the membrane-bound polysomes and the remaining supernatant fraction. Total...
Distribution of mutations around rearranged heavy-chain antibody variable-region genes.
Both, G W, Taylor, L, Pollard, J W, Steele, E J
The mechanism of somatic hypermutation in the variable region of immunoglobulin genes expressed in mammalian B cells is a major unexplained phenomenon in the generation of diversity in the immune...
Whitfeld, P L, Tyndall, C, Stirzaker, S C, Bellamy, A R, Both, G W
The Simian 11 rotavirus glycoprotein VP7 is directed to the endoplasmic reticulum (ER) of the cell and retained as an integral membrane protein. The gene coding for VP7 predicts two potential...
Both, G W, Shi, C H, Kilbourne, E D
The complete nucleotide sequence has been obtained of the H1 hemagglutinin (HA) gene of a high-yielding (H) mutant of the A/NJ/11/76(H1N1) strain of swine influenza virus in studies of a viral...
Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.
Both, G W, Mattick, J S, Bellamy, A R
Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus have been used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of...
Both, G W, Moyer, S A, Banerjee, A K
Vesicular stomatitis virus messenger RNA has been transcribed in vitro from the viral genome by the virion-associated RNA polymerase in quantities suitable for translation. Wheat germ cell-free...
Methylation-dependent translation of viral messenger RNAs in vitro.
Both, G W, Banerjee, A K, Shatkin, A J
Methylated reovirus and vesicular stomatitis virus mRNAs, synthesized in vitro in the presence of S-adenosylmethionine by the virion-associated polymerases (RNA nucleotidyltransferases, EC 2.7.7.6),...
Sleigh, M J, Both, G W, Underwood, P A, Bender, V J
The nucleotide sequence of the gene coding for the large subunit of influenza virus hemagglutinin (HA1) was determined for strains A/NT/60/68, A/Eng/878/69, and A/Qu/7/70, three early isolates of the...
The nucleotide sequence was determined for the hemagglutinin gene of the Hong Kong subtype influenza strain A/Bangkok/1/79. The amino acid sequence predicted from these data shows a total of 36 amino...
Clarke, M L, Lockett, L J, Both, G W
The sequences responsible for binding rotavirus glycoprotein VP7 to the membrane of the endoplasmic reticulum (ER) have not been identified. Here we show that the sequences which promote membrane...
Andrew, M E, Boyle, D B, Whitfeld, P L, Lockett, L J, Anthony, I D, Bellamy, A R, ...
The glycoprotein VP7, the major serotype antigen of rotaviruses, is localized to the endoplasmic reticulum (ER) of the cell, where it is retained as a membrane-associated protein before assembly into...
Andrew, M E, Boyle, D B, Coupar, B E, Whitfeld, P L, Both, G W, Bellamy, A R
A cDNA copy of the gene coding for the major outer neutralizing protein (VP7) of simian 11 rotavirus was incorporated into the vaccinia virus genome under the control of the vaccinia promoter...
Both, G W, Siegman, L J, Bellamy, A R, Ikegami, N, Shatkin, A J, Furuichi, Y
Cloned DNA copies of rotavirus genomic segment 6 from simian 11 (subgroup 1) and human strain Wa (subgroup 2) rotaviruses have been used to determine the nucleotide sequences of the gene that...
Gunn, P R, Sato, F, Powell, K F, Bellamy, A R, Napier, J R, Harding, D R, ...
The rotavirus neutralizing antigen, VP7, is a 37,000-molecular-weight glycoprotein which is a major component of the outer shell of the virion. The amino acid sequence of VP7 for strain S2 (human...
Both, G W, Sleigh, M J, Cox, N J, Kendal, A P
Surveys of the antigenic properties of a wide range of variants of the H3N2 (Hong Kong) influenza virus subtype have revealed complex patterns of variants cocirculating during each of the main...
Both, G W, Siegman, L J, Bellamy, A R, Atkinson, P H
A cloned DNA copy of simian rotavirus SA11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein NCVP5 to this gene. Determination of the nucleotide sequence for...
Nucleotide sequence of the dsRNA genomic segment 7 of Simian 11 rotavirus.
Both, G W, Bellamy, A R, Siegman, L J
A full length cDNA copy of dsRNA segment seven of Simian 11 rotavirus has been obtained by standard molecular cloning techniques. Segment seven codes for the non-structural viral protein NCVP4 and is...
The complete nucleotide sequence has been determined for a cloned double-stranded DNA copy of the haemagglutinin gene from the human influenza strain A/NT/60/68/29C, a laboratory-isolated variant of...
Both, G W, Bellamy, A R, Street, J E, Siegman, L J
Using Simian-11 rotavirus RNA, a strategy has been developed for the production of full length cloned copies of the genes of double-stranded (dsRNA) viruses. Genomic RNA segments were polyadenylated...
A new method for the size estimation of the RNA genome segments of influenza virus.
Sleigh, M J, Both, G W, Brownlee, G G
Previous estimates of the size of the RNA genome segments of influenza virus have been unreliable because of a lack of suitable RNA species as size markers. We have attempted to overcome this problem...
Influence of the sequence-dependent flexure of DNA on transcription in E. coli.
Collis, C M, Molloy, P L, Both, G W, Drew, H R
In order to study the effects of DNA structure on cellular processes such as transcription, we have made a series of plasmids that locate several different kinds of DNA structure (stiff, flexible or...
The influenza virus haemagglutinin gene: cloning and characterisation of a double-stranded DNA copy.
Sleigh, M J, Both, G W, Brownlee, G G
A protocol has been developed for the synthesis of a double-stranded DNA (dsDNA) copy of the influenza virus RNA genome segment which codes for the major surface antigen, haemagglutinin (HA). This...
Both, G W, Moyer, S A, Banerjee, A K
The cytoplasm of vesicular stomatitis virus (VSV)-infected BHK cells has been separated into a fraction containing the membrane-bound polysomes and the remaining supernatant fraction. Total...
Distribution of mutations around rearranged heavy-chain antibody variable-region genes.
Both, G W, Taylor, L, Pollard, J W, Steele, E J
The mechanism of somatic hypermutation in the variable region of immunoglobulin genes expressed in mammalian B cells is a major unexplained phenomenon in the generation of diversity in the immune...
Whitfeld, P L, Tyndall, C, Stirzaker, S C, Bellamy, A R, Both, G W
The Simian 11 rotavirus glycoprotein VP7 is directed to the endoplasmic reticulum (ER) of the cell and retained as an integral membrane protein. The gene coding for VP7 predicts two potential...
Both, G W, Shi, C H, Kilbourne, E D
The complete nucleotide sequence has been obtained of the H1 hemagglutinin (HA) gene of a high-yielding (H) mutant of the A/NJ/11/76(H1N1) strain of swine influenza virus in studies of a viral...
Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.
Both, G W, Mattick, J S, Bellamy, A R
Cloned DNA copies of the double-stranded RNA genomic segments of simian 11 rotavirus have been used to determine the coding assignment for VP7, the type-specific antigen of this virus. Translation of...
Both, G W, Moyer, S A, Banerjee, A K
Vesicular stomatitis virus messenger RNA has been transcribed in vitro from the viral genome by the virion-associated RNA polymerase in quantities suitable for translation. Wheat germ cell-free...
Methylation-dependent translation of viral messenger RNAs in vitro.
Both, G W, Banerjee, A K, Shatkin, A J
Methylated reovirus and vesicular stomatitis virus mRNAs, synthesized in vitro in the presence of S-adenosylmethionine by the virion-associated polymerases (RNA nucleotidyltransferases, EC 2.7.7.6),...