Fabiani, E, Stadler, AM, Madern, D, Koza, MM, Tehei, M, Hirai, M, ...
Stadler, A. M., Digel, I., Embs, J. P., Unruh, T., Tehei, M., Zaccai, G., ...
A transition in hemoglobin (Hb), involving partial unfolding and aggregation, has been shown previously by various biophysical methods. The correlation between the transition temperature and body...
Stadler, A. M., Digel, I., Embs, J. P., Unruh, T., Tehei, M., Zaccai, G., ...
A transition in hemoglobin (Hb), involving partial unfolding and aggregation, has been shown previously by various biophysical methods. The correlation between the transition temperature and body...
Fabiani, E., Stadler, A. M., Madern, D., Koza, M. M., Tehei, M., Hirai, M., ...
Changes of molecular dynamics in the α-to-β transition associated with amyloid fibril formation were explored on apo-myoglobin (ApoMb) as a model system. Circular dichroism, neutron and X-ray...
Down to atomic-scale intracellular water dynamics (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
Water constitutes the intracellular matrix in which biological molecules interact. Understanding its dynamic state is a main scientific challenge, which continues to provoke controversy after more...
In vivo measurement of internal and global macromolecular motions in E. coli (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in E. coli. The experiments were performed on a wide range of time-scales, to cover the large panel...
Solvent isotope effect on macromolecular dynamics in E. coli (2008)
Jasnin, M., Tehei, M., Moulin, M., Haertlein, M., Zaccai, G.
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in vivo. Measurements were performed on living E. coli bacteria containing H2O and...
Frölich, A., Gabel, F., Jasnin, M., Lehnert, U., Oesterhelt, D., Stadler, A., ...
An integrated picture of hydration shell dynamics and of its coupling to functional macromolecular motions is proposed from studies on a soluble protein, on a membrane protein in its natural lipid...
Solvent isotope effect on macromolecular dynamics in E. coli (2008)
Jasnin, M., Tehei, M., Moulin, M., Haertlein, M., Zaccai, G.
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in vivo. Measurements were performed on living E. coli bacteria containing H2O and...
Hydration dependence of active core fluctuations in bacteriorhodopsin (2008)
Wood, K., Lehnert, U., Kessler, B., Zaccai, G., Oesterhelt, D.
Dynamics of hydration water in deuterated purple membranes explored by neutron scattering (2008)
Wood, K., Plazanet, M., Gabel, F., Kessler, B., Oesterhelt, D., Zaccai, G., ...
In vivo measurement of internal and global macromolecular motions in E. coli (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in E. coli. The experiments were performed on a wide range of time-scales, to cover the large panel...
Dynamical heterogeneity of specific amino acids in bacteriorhodopsin (2008)
Wood, K., Grudinin, S., Kessler, B., Weik, M., Johnson, M., Kneller, G. R., ...
Fabiani, E., Stadler, A. M., Madern, D., Koza, M. M., Tehei, M., Hirai, M., ...
Changes of molecular dynamics in the α-to-β transition associated with amyloid fibril formation were explored on apo-myoglobin (ApoMb) as a model system. Circular dichroism, neutron and X-ray...
Down to atomic-scale intracellular water dynamics (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
Water constitutes the intracellular matrix in which biological molecules interact. Understanding its dynamic state is a main scientific challenge, which continues to provoke controversy after more...
Cytoplasmic water and hydration layer dynamics in human red blood cells (2008)
Stadler, AM, Embs, JP, Digel, I, Artmann, GM, Unruh, T, Buldt, G, ...
Frölich, A., Gabel, F., Jasnin, M., Lehnert, U., Oesterhelt, D., Stadler, A., ...
An integrated picture of hydration shell dynamics and of its coupling to functional macromolecular motions is proposed from studies on a soluble protein, on a membrane protein in its natural lipid...
In vivo measurement of internal and global macromolecular motions in E. coli (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in E. coli. The experiments were performed on a wide range of time-scales, to cover the large panel...
Down to atomic-scale intracellular water dynamics (2008)
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
Water constitutes the intracellular matrix in which biological molecules interact. Understanding its dynamic state is a main scientific challenge, which continues to provoke controversy after more...
Solvent isotope effect on macromolecular dynamics in E. coli (2008)
Jasnin, M., Tehei, M., Moulin, M., Haertlein, M., Zaccai, G.
Elastic incoherent neutron scattering was used to explore solvent isotope effects on average macromolecular dynamics in vivo. Measurements were performed on living E. coli bacteria containing H2O and...
Frölich, A., Gabel, F., Jasnin, M., Lehnert, U., Oesterhelt, D., Stadler, A., ...
An integrated picture of hydration shell dynamics and of its coupling to functional macromolecular motions is proposed from studies on a soluble protein, on a membrane protein in its natural lipid...
Fabiani, E., Stadler, A. M., Madern, D., Koza, M. M., Tehei, M., Hirai, M., ...
Changes of molecular dynamics in the α-to-β transition associated with amyloid fibril formation were explored on apo-myoglobin (ApoMb) as a model system. Circular dichroism, neutron and...
Erratum to "New sources and instrumentation for neutrons in biology" (V.345 PP. 133 2008) (2008)
Teixeira, SCM, Zaccai, G, Ankner, J, Bellissent-Funel, MC, Bewley, R, Blakeley, MP, ...
New sources and instrumentation for neutrons in biology (2008)
Teixeira, SCM, Zaccai, G, Ankner, J, Bellissent-Funel, MC, Bewley, R, Blakeley, MP, ...
Adaptation to high temperatures through macromolecular dynamics by neutron scattering (2007)
Work on the relationship between hyperthermophile protein dynamics, stability and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of...
Neutron scattering reveals extremely slow cell water in a Dead Sea organism (2007)
Tehei, M., Franzetti, B., Wood, K., Gabel, F., Fabiani, E., Jasnin, M., ...
Intracellular water dynamics in Haloarcula marismortui, an extremely halophilic organism originally isolated from the Dead Sea, was studied by neutron scattering. The water in centrifuged cell...
Neutron scattering reveals extremely slow cell water in a Dead Sea organism (2007)
Tehei, M., Franzetti, B., Wood, K., Gabel, F., Fabiani, E., Jasnin, M., ...
Coupling of protein and hydration-water dynamics in biological membranes (2007)
Wood, K., Plazanet, M., Gabel, F., Kessler, B., Oesterhelt, D., Tobias, D. J., ...
Adaptation to high temperatures through macromolecular dynamics by neutron scattering (2007)
Work on the relationship between hyperthermophile protein dynamics, stability and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of...
Neutron scattering reveals extremely slow cell water in a Dead Sea organism (2007)
Tehei, M., Franzetti, B., Wood, K., Gabel, F., Fabiani, E., Jasnin, M., ...
Intracellular water dynamics in Haloarcula marismortui, an extremely halophilic organism originally isolated from the Dead Sea, was studied by neutron scattering. The water in centrifuged cell...
Neutron scattering reveals extremely slow cell water in a Dead Sea organism (2007)
Tehei, M., Franzetti, B., Wood, K., Gabel, F., Fabiani, E., Jasnin, M., ...
Intracellular water dynamics in Haloarcula marismortui, an extremely halophilic organism originally isolated from the Dead Sea, was studied by neutron scattering. The water in centrifuged cell...
Adaptation to high temperatures through macromolecular dynamics by neutron scattering (2007)
Work on the relationship between hyperthermophile protein dynamics, stability and activity is reviewed. Neutron spectroscopy has been applied to measure and compare the macromolecular dynamics of...
Tehei, M., Daniel, R., Zaccai, G.
To explore macromolecular dynamics on the picosecond timescale, we used neutron spectroscopy. First, molecular dynamics were analyzed for the hyperthermophile malate dehydrogenase from Methanococcus...
Budyak, I. L., Pipich, V., Mironova, O. S., Schlesinger, R., Zaccai, G., Klein-Seetharaman, J.
This version is available at http://www.pnas.org, http://dx.doi.org/10.1073/pnas.0607201103
Tehei, M., Daniel, R., Zaccai, G.
To explore macromolecular dynamics on the picosecond timescale, we used neutron spectroscopy. First, molecular dynamics were analyzed for the hyperthermophile malate dehydrogenase from Methanococcus...
Tehei, M., Daniel, R., Zaccai, G.
To explore macromolecular dynamics on the picosecond timescale, we used neutron spectroscopy. First, molecular dynamics were analyzed for the hyperthermophile malate dehydrogenase from Methanococcus...
Neutron Scattering Reveals the Dynamic Basis of Protein Adaptation to Extreme Temperature (2005)
Tehei, M., Madern, D., Franzetti, B., Zaccai, G.
To explore protein adaptation to extremely high temperatures, two parameters related to macromolecular dynamics, the mean square atomic fluctuation and structural resilience, expressed as a mean...
Neutron Scattering Reveals the Dynamic Basis of Protein Adaptation to Extreme Temperature (2005)
Tehei, M., Madern, D., Franzetti, B., Zaccai, G.
To explore protein adaptation to extremely high temperatures, two parameters related to macromolecular dynamics, the mean square atomic fluctuation and structural resilience, expressed as a mean...
Neutron Scattering Reveals the Dynamic Basis of Protein Adaptation to Extreme Temperature (2005)
Tehei, M., Madern, D., Franzetti, B., Zaccai, G.
To explore protein adaptation to extremely high temperatures, two parameters related to macromolecular dynamics, the mean square atomic fluctuation and structural resilience, expressed as a mean...
Quantification of thermal motions in the purple membrane (2002)
Lehnert,U., Reat,V., Kessler,B., Oesterhelt,D., Zaccai,G.
Local atomic motions in bacteriorhodopsin (BR), the membrane protein in purple membranes (PMs) of Halobacterium salinarum, were studied by incoherent neutron scattering. The analysed sample consisted...
Protein dynamics studied by neutron scattering (2002)
Gabel,F., Bicout,D., Lehnert,U., Tehei,M., Weik,M., Zaccai,G.
This review of protein dynamics studied by neutron scattering focuses on data collected in the last 10 years. After an introduction to thermal neutron scattering and instrumental aspects, theoretical...
Protein dynamics studied by neutron scattering (2002)
Gabel,F., Bicout,D., Lehnert,U., Tehei,M., Weik,M., Zaccai,G.
Quantification of thermal motions in the purple membrane (2002)
Lehnert, U., Reat, V., Kessler, B., Oesterhelt, D., Zaccai, G.
Local atomic motions in bacteriorhodopsin (BR), the membrane protein in purple membranes (PMs) of Halobacterium salinarum, were studied by incoherent neutron scattering. The analysed sample consisted...
Protein dynamics studied by neutron scattering (2002)
Gabel, F., Bicout, D., Lehnert, U., Tehei, M., Weik, M., Zaccai, G.
This review of protein dynamics studied by neutron scattering focuses on data collected in the last 10 years. After an introduction to thermal neutron scattering and instrumental aspects, theoretical...
Protein dynamics studied by neutron scattering (2002)
Gabel, F., Bicout, D., Lehnert, U., Tehei, M., Weik, M., Zaccai, G.
B. Franzetti, G. Schoehn, D. Garcia, G. Zaccai
A 20S proteasome, comprising two subunits α and β, was purified from the extreme halophilic archaeon Haloarcula marismortui, which grows only in saturated salt conditions. The three-dimensional...
No abstract available
SALT AND WATER BINDING TO HALOPHILIC MALATE DEHYDROGENASE (1984)
Zaccai, G., Wachtel, E., Eisenberg, H.
No abstract available
No abstract available
SALT AND WATER BINDING TO HALOPHILIC MALATE DEHYDROGENASE (1984)
Zaccai, G., Wachtel, E., Eisenberg, H.
No abstract available
No abstract available
SALT AND WATER BINDING TO HALOPHILIC MALATE DEHYDROGENASE (1984)
Zaccai, G., Wachtel, E., Eisenberg, H.
No abstract available
A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs (1977)
Giegé, R., Jacrot, B., Moras, D., Thierry, J. C., Zaccai, G.
A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with...
KTN, STRUCTURAL AND DYNAMICAL STUDIES (1972)
Zaccai, G., Hewat, A., Rouse, K.
Sharp streaks along (100) planes have been observed in the scattering of X-rays and neutrons from KTaxNb1-xO3 crystals. The interpretation is in terms of a highly anisotropic transverse acoustic...
KTN, STRUCTURAL AND DYNAMICAL STUDIES (1972)
Zaccai, G., Hewat, A., Rouse, K.
Sharp streaks along (100) planes have been observed in the scattering of X-rays and neutrons from KTaxNb1-xO3 crystals. The interpretation is in terms of a highly anisotropic transverse acoustic...
KTN, STRUCTURAL AND DYNAMICAL STUDIES (1972)
Zaccai, G., Hewat, A., Rouse, K.
Sharp streaks along (100) planes have been observed in the scattering of X-rays and neutrons from KTaxNb1-xO3 crystals. The interpretation is in terms of a highly anisotropic transverse acoustic...
Protein hydration in solution: Experimental observation by x-ray and neutron scattering
Svergun, D. I., Richard, S., Koch, M. H. J., Sayers, Z., Kuprin, S., Zaccai, G.
The structure of the protein–solvent interface is the subject of controversy in theoretical studies and requires direct experimental characterization. Three proteins with known atomic resolution...
Tetrahedral aminopeptidase: a novel large protease complex from archaea
Franzetti, B., Schoehn, G., Jaquinod, M., Ruigrok, R.W.H., Zaccai, G.
A dodecameric protease complex with a tetrahedral shape (TET) was isolated from Haloarcula marismortui, a salt-loving archaeon. The 42 kDa monomers in the complex are homologous to metal-binding,...
Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.
Franzetti, B, Sohlberg, B, Zaccai, G, Von Gabain, A
Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An...
Structural changes in bacteriorhodopsin during proton translocation revealed by neutron diffraction.
Dencher, N A, Dresselhaus, D, Zaccai, G, Büldt, G
A neutron diffraction study of spectroscopic states for the light-energized proton pump bacteriorhodopsin (BR) is presented. The photocycle states BR-568 and M were generated at temperatures above 4...
Baldacci, G, Guinet, F, Tillit, J, Zaccai, G, De Recondo, A M
The primary structure of the gene for the elongation factor EF-Tu from the halophilic archaebacterium Halobacterium marismortui (hEF-Tu) is described. It is the first gene of a halophilic elongation...
A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs.
Giegé, R, Jacrot, B, Moras, D, Thierry, J C, Zaccai, G
A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with...
Bacteriorhodopsin is an inside-out protein.
Neutron scattering is particulary useful when parts of a structure can be deuterated. From Halobacterium halobium we have obtained, by biosynthetic incorporation, purple membranes in which all of the...
Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes
Zaccai, G., Blasie, J. K., Schoenborn, B. P.
Lamellar neutron diffraction from oriented multilayers of hydrated dipalmitoyl lecithin was phased by isomorphous H2O-D2O exchange and swelling techniques. Bound water sites were located in the polar...
Small angle neutron scattering studies of C8 and C9 and their interactions in solution.
Esser, A F, Thielens, N M, Zaccai, G
Small angle neutron scattering (SANS) results revealed that contrary to most reports C9 is not a globular protein. Its radius of gyration (Rg) at pH 8 and an ionic strength of 0.5 is 32.2 +/- 1.4 A...
Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature.
Cordone, L, Ferrand, M, Vitrano, E, Zaccai, G
Embedding biostructures in saccharide glasses protects them against extreme dehydration and/or exposure to very high temperature. Among the saccharides, trehalose appears to be the most effective...
Protein flexibility from the dynamical transition: a force constant analysis.
A standard analysis of the scattered neutron incoherent elastic intensity measured with very good energy resolution yields elastic scans, i.e., mean-square displacements of atomic motions (in a pico...
Biophysical characterization of the influence of salt on tetrameric SecB.
Dekker, C, Agianian, B, Weik, M, Zaccai, G, Kroon, J, Gros, P, ...
SecB is a tetrameric chaperone, with a monomeric molecular mass of 17 kDa, that is involved in protein translocation in Escherichia coli. It has been hypothesized that SecB undergoes a conformational...
Gabel, F., Weik, M., Doctor, B. P., Saxena, A., Fournier, D., Brochier, L., ...
A major result of incoherent elastic neutron-scattering experiments on protein powders is the strong dependence of the intramolecular dynamics on the sample environment. We performed a series of...
Assignment of segments of the bacteriorhodopsin sequence to positions in the structural map.
Trewhella, J, Anderson, S, Fox, R, Gogol, E, Khan, S, Engelman, D, ...
Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models...
Neutron diffraction analysis of cytochrome b5 reconstituted in deuterated lipid multilayers.
Gogol, E P, Engelman, D M, Zaccai, G
Cytochrome b5 was reconstituted with a highly deuterated phospholipid to form ordered multilayers consisting of repeated centrosymmetric pairs of asymmetric lipid-protein bilayers. Lamellar neutron...
Gabel, F., Weik, M., Masson, P., Renault, F., Fournier, D., Brochier, L., ...
Incoherent elastic neutron scattering experiments on members of the cholinesterase family were carried out to investigate how molecular dynamics is affected by covalent inhibitor binding and by...
Weik, M., Lehnert, U., Zaccai, G.
Confined water is of considerable current interest owing to its biophysical importance and relevance to cryopreservation. It can be studied in its amorphous or supercooled state in the “no-man's...
Protein hydration in solution: Experimental observation by x-ray and neutron scattering
Svergun, D. I., Richard, S., Koch, M. H. J., Sayers, Z., Kuprin, S., Zaccai, G.
The structure of the protein–solvent interface is the subject of controversy in theoretical studies and requires direct experimental characterization. Three proteins with known atomic resolution...
Tetrahedral aminopeptidase: a novel large protease complex from archaea
Franzetti, B., Schoehn, G., Jaquinod, M., Ruigrok, R.W.H., Zaccai, G.
A dodecameric protease complex with a tetrahedral shape (TET) was isolated from Haloarcula marismortui, a salt-loving archaeon. The 42 kDa monomers in the complex are homologous to metal-binding,...
Biochemical and serological evidence for an RNase E-like activity in halophilic Archaea.
Franzetti, B, Sohlberg, B, Zaccai, G, Von Gabain, A
Endoribonuclease RNase E appears to control the rate-limiting step that mediates the degradation of many mRNA species in bacteria. In this work, an RNase E-like activity in Archaea is described. An...
Structural changes in bacteriorhodopsin during proton translocation revealed by neutron diffraction.
Dencher, N A, Dresselhaus, D, Zaccai, G, Büldt, G
A neutron diffraction study of spectroscopic states for the light-energized proton pump bacteriorhodopsin (BR) is presented. The photocycle states BR-568 and M were generated at temperatures above 4...
Baldacci, G, Guinet, F, Tillit, J, Zaccai, G, De Recondo, A M
The primary structure of the gene for the elongation factor EF-Tu from the halophilic archaebacterium Halobacterium marismortui (hEF-Tu) is described. It is the first gene of a halophilic elongation...
A neutron investigation of yeast valyl-tRNA synthetase interaction with tRNAs.
Giegé, R, Jacrot, B, Moras, D, Thierry, J C, Zaccai, G
A new way of studying RNA-protein complexes, using neutron small angle scattering in solution, is described and was applied in the case of the system, yeast valyl-tRNA synthetase, interacting with...
Bacteriorhodopsin is an inside-out protein.
Neutron scattering is particulary useful when parts of a structure can be deuterated. From Halobacterium halobium we have obtained, by biosynthetic incorporation, purple membranes in which all of the...
Neutron Diffraction Studies on the Location of Water in Lecithin Bilayer Model Membranes
Zaccai, G., Blasie, J. K., Schoenborn, B. P.
Lamellar neutron diffraction from oriented multilayers of hydrated dipalmitoyl lecithin was phased by isomorphous H2O-D2O exchange and swelling techniques. Bound water sites were located in the polar...
Small angle neutron scattering studies of C8 and C9 and their interactions in solution.
Esser, A F, Thielens, N M, Zaccai, G
Small angle neutron scattering (SANS) results revealed that contrary to most reports C9 is not a globular protein. Its radius of gyration (Rg) at pH 8 and an ionic strength of 0.5 is 32.2 +/- 1.4 A...
Harmonic behavior of trehalose-coated carbon-monoxy-myoglobin at high temperature.
Cordone, L, Ferrand, M, Vitrano, E, Zaccai, G
Embedding biostructures in saccharide glasses protects them against extreme dehydration and/or exposure to very high temperature. Among the saccharides, trehalose appears to be the most effective...
Protein flexibility from the dynamical transition: a force constant analysis.
A standard analysis of the scattered neutron incoherent elastic intensity measured with very good energy resolution yields elastic scans, i.e., mean-square displacements of atomic motions (in a pico...
Biophysical characterization of the influence of salt on tetrameric SecB.
Dekker, C, Agianian, B, Weik, M, Zaccai, G, Kroon, J, Gros, P, ...
SecB is a tetrameric chaperone, with a monomeric molecular mass of 17 kDa, that is involved in protein translocation in Escherichia coli. It has been hypothesized that SecB undergoes a conformational...
Gabel, F., Weik, M., Doctor, B. P., Saxena, A., Fournier, D., Brochier, L., ...
A major result of incoherent elastic neutron-scattering experiments on protein powders is the strong dependence of the intramolecular dynamics on the sample environment. We performed a series of...
Assignment of segments of the bacteriorhodopsin sequence to positions in the structural map.
Trewhella, J, Anderson, S, Fox, R, Gogol, E, Khan, S, Engelman, D, ...
Specific amino acid sequence segments have been assigned to locations in the structural map of bacteriorhodopsin using two-dimensional neutron diffraction data and a model building analysis. Models...
Neutron diffraction analysis of cytochrome b5 reconstituted in deuterated lipid multilayers.
Gogol, E P, Engelman, D M, Zaccai, G
Cytochrome b5 was reconstituted with a highly deuterated phospholipid to form ordered multilayers consisting of repeated centrosymmetric pairs of asymmetric lipid-protein bilayers. Lamellar neutron...
Gabel, F., Weik, M., Masson, P., Renault, F., Fournier, D., Brochier, L., ...
Incoherent elastic neutron scattering experiments on members of the cholinesterase family were carried out to investigate how molecular dynamics is affected by covalent inhibitor binding and by...
Weik, M., Lehnert, U., Zaccai, G.
Confined water is of considerable current interest owing to its biophysical importance and relevance to cryopreservation. It can be studied in its amorphous or supercooled state in the “no-man's...
The effect of water on protein dynamics.
Neutron diffraction and spectroscopy were applied to describe the hydration and dynamics of a soluble protein and a natural membrane from extreme halophilic Archaea. The quantitative dependence of...
Coupling of protein and hydration-water dynamics in biological membranes
Wood, K., Plazanet, M., Gabel, F., Kessler, B., Oesterhelt, D., Tobias, D. J., ...
The dynamical coupling between proteins and their hydration water is important for the understanding of macromolecular function in a cellular context. In the case of membrane proteins, the...
Characterization of the proteasome from the extremely halophilic archaeon Haloarcula marismortui
Franzetti, B., Schoehn, G., Garcia, D., Ruigrok, R. W.H., Zaccai, G.
A 20S proteasome, comprising two subunits α and β, was purified from the extreme halophilic archaeon Haloarcula marismortui, which grows only in saturated salt conditions. The three-dimensional...
In Vivo Measurement of Internal and Global Macromolecular Motions in Escherichia coli
Jasnin, M., Moulin, M., Haertlein, M., Zaccai, G., Tehei, M.
We present direct quasielastic neutron scattering measurements, in vivo, of macromolecular dynamics in Escherichia coli. The experiments were performed on a wide range of timescales to cover the...
Stadler, A. M., Digel, I., Embs, J. P., Unruh, T., Tehei, M., Zaccai, G., ...
A transition in hemoglobin (Hb), involving partial unfolding and aggregation, has been shown previously by various biophysical methods. The correlation between the transition temperature and body...
Dynamics of hydration water in deuterated purple membranes explored by neutron scattering
Wood, K., Plazanet, M., Gabel, F., Kessler, B., Oesterhelt, D., Zaccai, G., ...
The function and dynamics of proteins depend on their direct environment, and much evidence has pointed to a strong coupling between water and protein motions. Recently however, neutron scattering...